##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933799.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	465626
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.57724010257159	30.0	16.0	33.0	16.0	34.0
2	27.822462663167435	31.0	25.0	33.0	16.0	34.0
3	28.786311331411905	31.0	28.0	34.0	16.0	34.0
4	32.35607332923849	35.0	30.0	35.0	25.0	37.0
5	33.74363115461765	35.0	33.0	37.0	28.0	37.0
6	32.82891634058235	35.0	32.0	37.0	25.0	37.0
7	33.55866081361436	35.0	32.0	35.0	29.0	37.0
8	33.307764171244735	35.0	33.0	37.0	28.0	37.0
9	34.65389175003114	37.0	32.0	39.0	28.0	39.0
10	33.78323590177524	35.0	32.0	39.0	27.0	39.0
11	34.338956587475785	37.0	32.0	39.0	27.0	39.0
12	34.33571149377397	37.0	32.0	39.0	27.0	39.0
13	34.71669107824735	37.0	34.0	39.0	27.0	39.0
14	34.500584159819255	37.0	33.0	39.0	25.0	40.0
15	34.881954615936394	37.0	32.0	39.0	27.0	40.0
16	34.25478173469695	36.0	32.0	39.0	27.0	40.0
17	34.24968107451044	36.0	32.0	38.0	27.0	40.0
18	34.28705226941794	36.0	32.0	39.0	27.0	40.0
19	33.88748910069455	36.0	32.0	39.0	25.0	40.0
20	34.95889190036639	37.0	33.0	39.0	27.0	40.0
21	34.352160317508044	37.0	32.0	39.0	25.0	40.0
22	35.43151799942443	37.0	34.0	39.0	29.0	40.0
23	36.1892763720239	38.0	34.0	39.0	30.0	40.0
24	36.308268868147394	38.0	35.0	40.0	31.0	40.0
25	34.3323761989236	37.0	32.0	39.0	25.0	40.0
26	35.137614738008615	37.0	33.0	39.0	27.0	40.0
27	35.56645247473294	37.0	34.0	39.0	30.0	40.0
28	35.79118434108061	38.0	34.0	40.0	30.0	40.0
29	36.122295576277956	38.0	35.0	40.0	30.0	41.0
30	34.189693015424396	37.0	32.0	39.0	24.0	40.0
31	34.71008921323122	37.0	33.0	39.0	26.0	40.0
32	35.13705849759249	37.0	33.0	39.0	29.0	40.0
33	35.91704501037313	38.0	35.0	39.0	30.0	40.0
34	36.14309123631412	38.0	35.0	40.0	30.0	40.0
35	36.41773225721931	38.0	35.0	40.0	31.0	41.0
36	36.35443252739323	38.0	35.0	40.0	30.0	41.0
37	36.42985357346883	38.0	35.0	40.0	31.0	41.0
38	36.03142865733443	38.0	35.0	40.0	30.0	41.0
39	35.73497184435577	38.0	34.0	40.0	30.0	41.0
40	35.53895615794651	37.0	34.0	40.0	30.0	41.0
41	34.87818764416076	37.0	33.0	39.0	27.0	40.0
42	35.89386546283927	38.0	34.0	40.0	30.0	41.0
43	36.0651445580788	38.0	35.0	40.0	31.0	40.0
44	36.48690794758025	38.0	35.0	40.0	31.0	41.0
45	36.70610962446255	39.0	35.0	40.0	32.0	41.0
46	36.45046668356149	38.0	35.0	40.0	31.0	41.0
47	36.20988089153097	38.0	35.0	40.0	31.0	41.0
48	36.319563340535105	38.0	35.0	40.0	31.0	41.0
49	36.516968554161494	38.0	35.0	40.0	31.0	41.0
50	36.32099796832651	38.0	35.0	40.0	31.0	41.0
51	35.63800560965238	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	10.0
11	10.0
12	9.0
13	17.0
14	10.0
15	38.0
16	53.0
17	118.0
18	211.0
19	422.0
20	539.0
21	831.0
22	1060.0
23	1586.0
24	2140.0
25	3066.0
26	4301.0
27	5915.0
28	7971.0
29	11061.0
30	15725.0
31	22292.0
32	30509.0
33	40410.0
34	51001.0
35	65912.0
36	80080.0
37	77588.0
38	39244.0
39	3493.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.72851601929445	3.8842332687607652	45.1946411927169	16.192609519227876
2	17.453063188052216	17.677921765537146	44.40666973064219	20.46234531576845
3	18.124846980194405	17.40710355521384	46.29745761619841	18.170591848393347
4	18.47147710823708	3.812287114551163	55.173680163908365	22.54255561330338
5	26.674197746689405	4.399668403396718	44.657729594137784	24.268404255776094
6	19.168173598553345	15.677389149231358	46.558611417747294	18.59582583446801
7	67.4116136126419	1.5639161043412526	24.54652446384008	6.477945819176764
8	64.48995545781379	11.695008440250328	12.962764106815342	10.852271995120548
9	60.055495182829134	4.289708908007714	15.828153926112373	19.826641983050774
10	35.83562773556459	22.424220296976543	23.491385790312396	18.248766177146468
11	27.756396764785475	22.322421857885942	27.844020737673585	22.077160639655002
12	21.72730904202085	18.390510839171352	33.80567236365667	26.07650775515113
13	21.57074561987518	22.363441904017385	38.31207879285091	17.75373368325652
14	19.913836426660023	25.65642812042283	28.97432703500234	25.45540841791481
15	13.970010265749766	24.3824442793143	39.76818304819748	21.879362406738455
16	15.400772293643398	25.076993123236246	27.91897359683523	31.603260986285132
17	15.955294592655909	26.5431913166361	32.342051345929995	25.159462744778
18	15.605872524300619	23.37090282759124	34.848139923457886	26.175084724650254
19	16.514541713735916	26.119675447676894	29.966110139897683	27.39967269868951
20	18.947825078496475	25.98415896019552	37.20625566441736	17.861760296890637
21	21.847577240102574	29.16718568121196	28.354086756323742	20.631150322361723
22	17.433519605863935	24.070391258220116	31.075369502562143	27.42071963335381
23	18.083397404784098	28.15693281732549	28.456744253972072	25.30292552391834
24	22.896272974447303	22.256274348940998	25.988883782263017	28.858568894348686
25	16.448823734069833	29.777332021837267	23.577076881445624	30.196767362647275
26	17.62637825207355	21.937778388663865	28.401764506277573	32.03407885298502
27	23.64859350637636	24.882631124550606	25.700454871506317	25.768320497566716
28	18.610859359228222	23.257507097971335	30.865329685197988	27.266303857602452
29	22.261428700287354	21.974503142006675	25.343730805410353	30.42033735229562
30	27.49674631571261	23.932512359705	28.233389028963156	20.337352295619233
31	26.034199121183093	23.586311760941182	25.992749545772785	24.386739572102933
32	23.02341364099084	31.162134416892528	25.15774462766254	20.65670731445409
33	24.009827629900393	24.116565655697922	24.583893511101184	27.289713203300504
34	22.258636759974742	21.923603922461375	30.929114783109192	24.88864453445469
35	20.131822535683146	23.294231851314144	28.071886020110558	28.50205959289215
36	25.566871265779834	24.7685911010124	24.747114637069238	24.917422996138534
37	23.234527281552147	25.40601255084553	32.38156803958542	18.977892128016908
38	27.47763226280319	26.405741947399846	24.20547821642262	21.91114757337434
39	22.54491802433713	27.574491115186866	30.691799856537223	19.188791003938782
40	25.491059348060464	19.949272592166245	32.68825194469381	21.871416115079484
41	19.341703427214117	25.478817763612856	29.904687453020234	25.274791356152793
42	20.506157302212504	20.942559049537614	33.97641025200483	24.574873396245057
43	24.68354430379747	24.346793349168646	24.410793211719277	26.558869135314612
44	22.765051779754568	19.467770270560493	30.099264216345308	27.66791373333963
45	22.5440589657794	17.47110341776447	27.764772585723307	32.22006503073282
46	26.715862086739143	22.510985211306927	29.641386005077035	21.13176669687689
47	17.651935244165916	19.505783611739894	39.69752548182447	23.14475566226972
48	20.49520430560149	22.05439558787525	29.83381512200779	27.61658498451547
49	21.295632116763237	17.214244909004222	38.9132050186201	22.576917955612444
50	24.305343773758338	17.389707619419877	31.09448355547156	27.210465051350223
51	19.528548663519647	16.479535077508558	28.801441500259866	35.19047475871193
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	63.0
1	188.5
2	314.0
3	1031.0
4	1748.0
5	1203.5
6	659.0
7	659.0
8	659.0
9	826.5
10	994.0
11	1159.0
12	1324.0
13	1291.0
14	1258.0
15	1170.0
16	1082.0
17	1041.5
18	1001.0
19	1028.0
20	1055.0
21	1134.0
22	1213.0
23	1325.0
24	1437.0
25	1510.0
26	2194.5
27	2806.0
28	3116.5
29	3427.0
30	3866.0
31	4305.0
32	4947.5
33	5590.0
34	6281.0
35	6972.0
36	7290.0
37	7608.0
38	9022.0
39	10436.0
40	11828.0
41	13220.0
42	15779.5
43	18339.0
44	22461.0
45	26583.0
46	61130.0
47	95677.0
48	84200.0
49	72723.0
50	69308.0
51	65893.0
52	50856.0
53	35819.0
54	30077.5
55	24336.0
56	20872.0
57	17408.0
58	15060.5
59	12713.0
60	10947.5
61	9182.0
62	8117.0
63	7052.0
64	5733.5
65	4415.0
66	3503.5
67	2592.0
68	2082.5
69	1573.0
70	1282.0
71	991.0
72	782.0
73	573.0
74	514.0
75	364.5
76	274.0
77	182.0
78	90.0
79	83.0
80	76.0
81	78.5
82	81.0
83	53.5
84	26.0
85	13.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	465626.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.793705521200792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.78576005769122	22.18747904847411
2	12.86685041503319	8.18169706161811
3	5.295287158605257	5.050704017126749
4	2.587818333252895	3.2910533611922888
5	1.5984547697149922	2.5410400118638647
6	1.0719327049219276	2.0448427655299195
7	0.8171976142048785	1.8187218209060436
8	0.6264454779075337	1.593361843974402
9	0.5490414973611856	1.571045731741861
>10	4.593269203928444	25.477860048464642
>50	0.11249362825392938	2.469797476789936
>100	0.0784046499951629	4.548554524570829
>500	0.006817795651753295	1.616401595964767
>1k	0.006817795651753295	6.314739344729194
>5k	0.0013635591303506592	3.029913035858323
>10k+	0.0020453386955259886	8.262788311194978
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGC	14777	3.173577076881445	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCG	12317	2.6452560638795943	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	11025	2.367780149733907	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	8301	1.7827612719221004	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	5677	1.2192188580534593	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGAGTTAT	4787	1.0280783289592934	No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	4659	1.0005884551120428	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	3903	0.8382263877017176	TruSeq Adapter, Index 22 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	3679	0.7901191084690288	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTA	3156	0.6777972020462775	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTC	2782	0.5974752268988416	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT	2585	0.5551665929308072	No Hit
TGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTT	1490	0.3199993127531538	TruSeq Adapter, Index 22 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGT	1054	0.22636192996095578	No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	1037	0.22271093109061782	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	988	0.2121874637584671	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATG	978	0.21003981736415064	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	832	0.17868418000713018	No Hit
TGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	827	0.17761035680997198	No Hit
TCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	789	0.16944930051156937	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTAT	743	0.1595701270977136	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT	646	0.13873795707284386	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCGGAGTTA	572	0.12284537375490201	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCC	551	0.11833531632683741	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	531	0.11404002353820447	No Hit
TTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	500	0.10738231971582342	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.131809649804779	0.0
2	0.0	0.0	0.0	5.260230313599327	0.0
3	0.0	0.0	0.0	6.483744464441418	0.0
4	0.0	0.0	0.0	8.86655813893554	0.0
5	0.0	0.0	0.0	13.592668794268361	0.0
6	0.0	0.0	0.0	14.86772645857405	0.0
7	0.0	0.0	0.0	16.622783092009467	0.0
8	0.0	0.0	0.0	18.90916744339878	0.0
9	0.0	0.0	0.0	19.61080352042197	0.0
10	0.0	0.0	0.0	23.84918367960552	0.0
11	0.0	0.0	0.0	26.62286899786524	0.0
12	0.0	0.0	0.0	32.36374257451259	0.0
13	0.0	0.0	0.0	33.41737789556425	0.0
14	0.0	0.0	0.0	33.85807493567799	0.0
15	0.0	0.0	0.0	35.02489122171013	0.0
16	0.0	0.0	0.0	36.17044580843853	0.0
17	0.0	0.0	0.0	37.62075141852044	0.0
18	0.0	0.0	0.0	38.99975516831105	0.0
19	0.0	0.0	0.0	41.148045856545814	0.0
20	0.0	0.0	0.0	42.32323796351578	0.0
21	0.0	0.0	0.0	43.76967781008793	0.0
22	0.0	0.0	0.0	45.28290945952331	0.0
23	0.0	0.0	0.0	46.637215275779276	0.0
24	0.0	0.0	0.0	47.70008547632649	0.0
25	0.0	0.0	0.0	48.56601650251489	0.0
26	0.0	0.0	0.0	49.38319595555231	0.0
27	0.0	0.0	0.0	50.1486171304867	0.0
28	0.0	0.0	0.0	50.948615412369584	0.0
29	0.0	0.0	0.0	51.753768045598825	0.0
30	0.0	0.0	0.0	52.63795406613892	0.0
31	0.0	0.0	0.0	53.37760348434151	0.0
32	0.0	0.0	0.0	54.1043670241782	0.0
33	2.1476463943164687E-4	0.0	0.0	54.80965410007173	0.0
34	2.1476463943164687E-4	0.0	0.0	55.53727669846615	0.0
35	2.1476463943164687E-4	0.0	0.0	56.28014758626022	0.0
36	2.1476463943164687E-4	0.0	0.0	56.97555548873989	0.0
37	2.1476463943164687E-4	0.0	0.0	57.64433257593004	0.0
38	2.1476463943164687E-4	0.0	0.0	58.3668008229781	0.0
39	2.1476463943164687E-4	0.0	0.0	59.08046371980946	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCCTC	25	3.887218E-5	45.000004	9
GCACCTA	65	0.0	45.000004	9
GCCGATG	25	3.887218E-5	45.000004	9
TCGCTCG	25	3.887218E-5	45.000004	1
GATTGTA	25	3.887218E-5	45.000004	20
TACCGGG	25	3.887218E-5	45.000004	3
GGCTAAT	25	3.887218E-5	45.000004	8
CTATGCG	20	7.028958E-4	45.0	1
TCGTTAG	20	7.028958E-4	45.0	1
CCGGGTC	20	7.028958E-4	45.0	5
TGCCCCG	20	7.028958E-4	45.0	1
GGTAATC	40	6.7993824E-9	45.0	8
CCGGCAG	40	6.7993824E-9	45.0	11
TATGGCG	20	7.028958E-4	45.0	1
CGGTTCG	20	7.028958E-4	45.0	33
CGCATCC	20	7.028958E-4	45.0	45
CACGAGG	20	7.028958E-4	45.0	15
CGATTAT	20	7.028958E-4	45.0	10
CGTTAGG	20	7.028958E-4	45.0	2
CTTTGCG	85	0.0	45.0	1
>>END_MODULE