SRR2933798.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933798.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	376477
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGC	12623	3.352927270457425	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCG	10848	2.8814509252889287	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC	9467	2.5146290477240307	No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	5592	1.485349702637877	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGGTACAT	4205	1.1169341022160717	No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	3829	1.0170608031831958	No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	2759	0.7328468937013417	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT	2726	0.7240814179883499	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGGTACATCGTA	2693	0.7153159422753581	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTC	2234	0.5933961437219273	No Hit
TCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	2144	0.5694903008683133	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCT	2138	0.5678965780114058	No Hit
TGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCTT	1234	0.3277756675706617	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGGTACATCGT	923	0.24516769948761807	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATG	809	0.21488696520637385	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG	797	0.21169951949255866	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	712	0.18912177901970106	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTAT	609	0.16176286997612072	No Hit
TCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG	606	0.16096600854766693	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTTGGTACA	541	0.14370067759783467	No Hit
TGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG	529	0.14051323188401948	No Hit
TTCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT	390	0.10359198569899356	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGC	385	0.10226388331823724	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTTA	25	3.8857906E-5	45.0	20
CAGGTAA	25	3.8857906E-5	45.0	24
GGTACTC	20	7.0272374E-4	45.0	9
CTCGTCG	20	7.0272374E-4	45.0	1
TTTACGA	35	1.2090095E-7	45.0	15
CGTGCGC	20	7.0272374E-4	45.0	17
CTCCCAT	20	7.0272374E-4	45.0	26
CGACCGG	20	7.0272374E-4	45.0	2
AATGGTG	20	7.0272374E-4	45.0	15
CGATTCA	20	7.0272374E-4	45.0	10
TGCGGAT	20	7.0272374E-4	45.0	4
GCTCTCA	20	7.0272374E-4	45.0	28
GCACACG	25	3.8857906E-5	45.0	12
TCGCTTC	20	7.0272374E-4	45.0	14
CGAGGGA	20	7.0272374E-4	45.0	4
TCGCTAG	35	1.2090095E-7	45.0	32
CAAGCGT	25	3.8857906E-5	45.0	31
ATGCGAC	20	7.0272374E-4	45.0	13
TACTAGA	20	7.0272374E-4	45.0	15
TTACGAT	25	3.8857906E-5	45.0	16

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content