##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933798.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	376477
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.680862310313778	31.0	16.0	33.0	16.0	34.0
2	27.886240062473934	31.0	25.0	33.0	16.0	34.0
3	28.77093155757191	31.0	28.0	34.0	16.0	34.0
4	32.32968547879419	35.0	28.0	37.0	25.0	37.0
5	33.74463513043293	35.0	33.0	37.0	28.0	37.0
6	32.88471274473606	35.0	32.0	37.0	26.0	37.0
7	33.54999375791881	35.0	32.0	35.0	29.0	37.0
8	33.331728100255795	35.0	33.0	37.0	28.0	37.0
9	34.779864374184875	37.0	32.0	39.0	29.0	39.0
10	33.69525894012118	35.0	32.0	39.0	27.0	39.0
11	34.06794306159473	35.0	32.0	39.0	27.0	39.0
12	34.13989699237935	37.0	32.0	39.0	26.0	39.0
13	34.57091668282524	37.0	33.0	39.0	27.0	39.0
14	34.428520201765316	37.0	32.0	39.0	25.0	40.0
15	34.887477853892804	37.0	32.0	39.0	27.0	40.0
16	34.39489796189409	36.0	32.0	39.0	27.0	40.0
17	34.34894296331515	36.0	32.0	38.0	27.0	40.0
18	34.34317368657315	36.0	32.0	39.0	27.0	40.0
19	34.043150046350775	36.0	32.0	39.0	25.0	40.0
20	34.94064179219448	37.0	33.0	39.0	27.0	40.0
21	34.346557691439315	37.0	32.0	39.0	25.0	40.0
22	35.39654480884622	37.0	34.0	39.0	28.0	40.0
23	36.17690058091198	38.0	34.0	39.0	30.0	40.0
24	36.326253662242316	38.0	35.0	40.0	31.0	40.0
25	34.365801894936475	37.0	32.0	39.0	25.0	40.0
26	34.94785073191722	37.0	33.0	39.0	27.0	40.0
27	35.64161954116719	37.0	34.0	39.0	30.0	40.0
28	35.80991136244711	38.0	34.0	40.0	30.0	40.0
29	36.29691853685617	38.0	35.0	40.0	30.0	41.0
30	34.382984883538704	37.0	33.0	39.0	25.0	40.0
31	35.155310948610406	37.0	33.0	39.0	28.0	40.0
32	35.38997335826624	37.0	34.0	39.0	30.0	40.0
33	35.99186670102025	38.0	35.0	40.0	30.0	40.0
34	36.33494210801722	38.0	35.0	40.0	30.0	41.0
35	36.51649104726185	38.0	35.0	40.0	31.0	41.0
36	36.42020893706654	38.0	35.0	40.0	30.0	41.0
37	36.77372854118578	38.0	35.0	40.0	32.0	41.0
38	36.17607450123115	38.0	35.0	40.0	30.0	41.0
39	36.17733885469763	38.0	35.0	40.0	30.0	41.0
40	35.61149021055735	37.0	34.0	40.0	30.0	41.0
41	35.2023178042749	37.0	33.0	39.0	29.0	41.0
42	36.23373805039883	38.0	35.0	40.0	31.0	41.0
43	36.31307888662521	38.0	35.0	40.0	31.0	41.0
44	36.79585207064442	38.0	35.0	40.0	32.0	41.0
45	37.014022104936025	39.0	35.0	40.0	33.0	41.0
46	36.819327608326674	39.0	35.0	40.0	32.0	41.0
47	36.57945372492875	38.0	35.0	40.0	31.0	41.0
48	36.70703655203372	38.0	35.0	40.0	32.0	41.0
49	36.94007070817075	39.0	35.0	40.0	33.0	41.0
50	36.79576972829682	39.0	35.0	40.0	32.0	41.0
51	36.07195658698938	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	9.0
11	8.0
12	12.0
13	8.0
14	17.0
15	20.0
16	46.0
17	97.0
18	176.0
19	281.0
20	423.0
21	634.0
22	912.0
23	1221.0
24	1727.0
25	2265.0
26	3180.0
27	4371.0
28	6069.0
29	8555.0
30	12254.0
31	17478.0
32	24426.0
33	32468.0
34	39436.0
35	50837.0
36	62933.0
37	64715.0
38	38264.0
39	3630.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.45788188919908	3.6012824156588583	45.276072641887815	16.66476305325425
2	17.662964802630704	18.14081603922683	44.27574592870215	19.92047322944031
3	18.094066835424208	18.35039059491018	45.550458593752076	18.005083975913536
4	17.91849170068822	3.68973403421723	55.89531365793926	22.49646060715528
5	27.092757326476786	4.635608549791886	44.15834167824329	24.11329244548804
6	19.258015761919054	16.07721056000765	46.85093644498867	17.813837233084623
7	69.25097681930104	1.6566749097554432	23.235948012760407	5.856400258183102
8	66.59370957588379	12.148683717730432	12.037919979175355	9.219686727210426
9	62.21734661081554	3.9248081556110996	14.127025024105059	19.73082020946831
10	38.78058951808477	22.226058962433296	21.422557022075715	17.57079449740622
11	31.666476305325425	21.412197823505817	26.543985422748268	20.37734044842049
12	23.892827450282486	17.925132212592004	32.28404391237712	25.897996424748392
13	22.157794500062423	21.794691309163643	37.770700467757656	18.27681372301628
14	20.09020471370097	26.208772381845375	29.01292774857428	24.688095155879378
15	15.199866127280021	23.203542314669953	40.06141145408617	21.535180103963857
16	16.840338187990238	23.44844439368142	28.83283706574372	30.87838035258462
17	17.85341468403116	25.050135864873553	31.936081088618963	25.160368362476326
18	17.116849103663704	23.136605954679833	33.93540641260954	25.81113852904693
19	18.421045641566415	25.69824982668264	29.430483137084074	26.450221394666872
20	20.99278309166297	24.79832765348215	36.01733970468315	18.19154955017172
21	22.199231294342017	29.46182635326992	28.476374386748727	19.862567965639336
22	17.9240697306874	22.746940716165927	31.742443761504685	27.586545791641985
23	19.309280513816248	27.683497265437197	28.813181150508534	24.194041070238022
24	23.08985675087721	21.453368997309262	27.097272874571352	28.359501377242168
25	16.603404723263306	30.03237913604284	25.384552044348	27.979664096345857
26	17.49137397503699	22.1426541329218	32.9730634275135	27.392908464527714
27	20.800739487405608	25.56783017289237	29.215064930925394	24.416365408776635
28	17.72060444595553	23.07020083564202	35.05738730387248	24.151807414529973
29	23.38522672035742	18.5966207763024	32.74462981802341	25.273522685316767
30	19.701070716139366	24.217946913091637	36.1424469489504	19.9385354218186
31	27.21759895026788	24.14304193881698	24.779202979199262	23.860156131715883
32	25.53967440242033	26.588344042265533	25.987244904735217	21.88473665057892
33	19.361873368094198	24.34491350069194	27.957883217301454	28.335329913912403
34	23.54672396985739	24.52526980399865	29.5829492903949	22.345056935749064
35	17.489249011227777	21.845690440584683	31.051299282559093	29.613761265628447
36	21.765207436310853	26.118727040430095	32.12599972906711	19.990065794191942
37	22.257667799095294	22.433774174783586	35.514785763804966	19.793772262316157
38	24.916528765369463	24.74121925110963	26.68051434749002	23.661737636030885
39	25.948995556169436	26.224709610414447	28.977068984293865	18.849225849122256
40	19.34460803714437	21.322683723042843	33.620380527894135	25.712327711918658
41	18.278673066349338	31.258748874433234	24.823827219192673	25.638750840024755
42	19.92684812086794	20.692100712659737	30.215922885063364	29.165128281408958
43	23.355211606552327	24.116479891201852	27.02502410505821	25.50328439718761
44	21.157467786876754	19.902676657538176	32.93189225371005	26.007963301875016
45	21.245388164482822	18.64602618486654	29.293422971390022	30.81516267926062
46	27.28028538263958	21.990984841039428	29.67804142085705	21.050688355463944
47	15.708529339109692	19.742773130895113	41.33453039627919	23.214167133716003
48	20.198843488446837	23.57594222223403	29.67591645704784	26.549297832271296
49	20.442948706029853	17.807727962133143	37.472939913992086	24.276383417844915
50	23.499709145578613	18.89225636625876	30.684743025470347	26.923291462692276
51	19.075003253850834	18.397405419188956	28.184723103934633	34.342868223025576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	44.0
1	166.5
2	289.0
3	821.0
4	1353.0
5	965.0
6	577.0
7	565.5
8	554.0
9	606.5
10	659.0
11	701.0
12	743.0
13	769.0
14	795.0
15	741.0
16	687.0
17	645.0
18	603.0
19	659.0
20	715.0
21	868.0
22	1021.0
23	1042.5
24	1064.0
25	1275.0
26	1802.5
27	2119.0
28	2406.0
29	2693.0
30	3418.0
31	4143.0
32	4674.0
33	5205.0
34	5757.0
35	6309.0
36	6875.5
37	7442.0
38	8851.5
39	10261.0
40	12074.0
41	13887.0
42	17044.5
43	20202.0
44	47635.5
45	75069.0
46	63879.5
47	52690.0
48	49994.5
49	47299.0
50	38031.5
51	28764.0
52	24903.0
53	21042.0
54	18447.5
55	15853.0
56	14467.5
57	13082.0
58	11977.5
59	10873.0
60	9808.0
61	8743.0
62	7520.5
63	6298.0
64	5595.0
65	4892.0
66	3979.0
67	3066.0
68	2505.5
69	1945.0
70	1705.0
71	1465.0
72	1226.5
73	988.0
74	838.5
75	553.0
76	417.0
77	318.0
78	219.0
79	173.5
80	128.0
81	104.5
82	81.0
83	51.0
84	21.0
85	11.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	376477.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.771733164476984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.94434611313876	18.31388783960005
2	14.52036145095062	8.06511207335117
3	5.838246027327157	4.864146324584929
4	2.898965144751876	3.22037145812674
5	1.655275316138648	2.298493219677391
6	1.0934502861869353	1.8220205745962736
7	0.7618179204635789	1.4809902804921886
8	0.6194111187076315	1.3761696246286819
9	0.5178354401960605	1.294306890141103
>10	5.911290229750441	32.31259007686135
>50	0.14539224603621656	2.7224692221839755
>100	0.07269612301810828	3.4182973980286406
>500	0.007966698412943373	1.5282776230992128
>1k	0.008962535714561294	6.626961347213777
>5k	0.0019916746032358434	4.164736287776157
>10k+	0.0019916746032358434	6.491169759638368
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGC	12623	3.352927270457425	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCG	10848	2.8814509252889287	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC	9467	2.5146290477240307	No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	5592	1.485349702637877	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGGTACAT	4205	1.1169341022160717	No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	3829	1.0170608031831958	No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	2759	0.7328468937013417	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT	2726	0.7240814179883499	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGGTACATCGTA	2693	0.7153159422753581	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTC	2234	0.5933961437219273	No Hit
TCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	2144	0.5694903008683133	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCT	2138	0.5678965780114058	No Hit
TGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCTT	1234	0.3277756675706617	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGGTACATCGT	923	0.24516769948761807	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATG	809	0.21488696520637385	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG	797	0.21169951949255866	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	712	0.18912177901970106	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTAT	609	0.16176286997612072	No Hit
TCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG	606	0.16096600854766693	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTTGGTACA	541	0.14370067759783467	No Hit
TGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG	529	0.14051323188401948	No Hit
TTCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT	390	0.10359198569899356	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGC	385	0.10226388331823724	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8308608494011587	0.0
2	0.0	0.0	0.0	4.125351615105306	0.0
3	0.0	0.0	0.0	5.107616135912685	0.0
4	0.0	0.0	0.0	7.018755461821041	0.0
5	0.0	0.0	0.0	11.409196312125308	0.0
6	0.0	0.0	0.0	12.368617471983681	0.0
7	0.0	0.0	0.0	13.87123250557139	0.0
8	0.0	0.0	0.0	15.897119877177092	0.0
9	0.0	0.0	0.0	16.496359671374346	0.0
10	0.0	0.0	0.0	20.707772320752664	0.0
11	0.0	0.0	0.0	22.948015416612435	0.0
12	0.0	0.0	0.0	28.543045126262694	0.0
13	0.0	0.0	0.0	29.49131022612271	0.0
14	0.0	0.0	0.0	29.89638145225339	0.0
15	0.0	0.0	0.0	31.05448672827291	0.0
16	0.0	0.0	0.0	32.151764915253786	0.0
17	0.0	0.0	0.0	33.370697280311944	0.0
18	0.0	0.0	0.0	34.66028469202634	0.0
19	0.0	0.0	0.0	36.74620229124223	0.0
20	0.0	0.0	0.0	37.849324128698434	0.0
21	0.0	0.0	0.0	39.028679042810055	0.0
22	0.0	0.0	0.0	40.34164105642576	0.0
23	0.0	0.0	0.0	41.50877742863442	0.0
24	0.0	0.0	0.0	42.444292745639174	0.0
25	0.0	0.0	0.0	43.232919939332284	0.0
26	0.0	0.0	0.0	43.94000164684695	0.0
27	0.0	0.0	0.0	44.61334955389041	0.0
28	0.0	0.0	0.0	45.35735250759011	0.0
29	0.0	0.0	0.0	46.118089551287326	0.0
30	0.0	0.0	0.0	46.96595011116217	0.0
31	0.0	0.0	0.0	47.70012510724427	0.0
32	0.0	0.0	0.0	48.37772294190614	0.0
33	0.0	0.0	0.0	49.05638325847263	0.0
34	0.0	0.0	0.0	49.70051291313945	0.0
35	0.0	0.0	0.0	50.38926680779968	0.0
36	0.0	0.0	0.0	51.06898960627077	0.0
37	0.0	0.0	0.0	51.71365050188989	0.0
38	0.0	0.0	0.0	52.41063863131081	0.0
39	0.0	0.0	0.0	53.07415858073667	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTTA	25	3.8857906E-5	45.0	20
CAGGTAA	25	3.8857906E-5	45.0	24
GGTACTC	20	7.0272374E-4	45.0	9
CTCGTCG	20	7.0272374E-4	45.0	1
TTTACGA	35	1.2090095E-7	45.0	15
CGTGCGC	20	7.0272374E-4	45.0	17
CTCCCAT	20	7.0272374E-4	45.0	26
CGACCGG	20	7.0272374E-4	45.0	2
AATGGTG	20	7.0272374E-4	45.0	15
CGATTCA	20	7.0272374E-4	45.0	10
TGCGGAT	20	7.0272374E-4	45.0	4
GCTCTCA	20	7.0272374E-4	45.0	28
GCACACG	25	3.8857906E-5	45.0	12
TCGCTTC	20	7.0272374E-4	45.0	14
CGAGGGA	20	7.0272374E-4	45.0	4
TCGCTAG	35	1.2090095E-7	45.0	32
CAAGCGT	25	3.8857906E-5	45.0	31
ATGCGAC	20	7.0272374E-4	45.0	13
TACTAGA	20	7.0272374E-4	45.0	15
TTACGAT	25	3.8857906E-5	45.0	16
>>END_MODULE