SRR2933797.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933797.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	768501
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGC	12336	1.6052028559494391	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCG	11018	1.4337001513335703	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	10074	1.3108636163127958	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	9753	1.2690939894678082	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	8703	1.13246436894682	No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	8103	1.0543903000776838	No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	6004	0.7812611824838225	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTCAAAGT	4398	0.572282924810768	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	3410	0.44372095807292383	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTA	2728	0.35497676645833903	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTC	2601	0.3384510885477052	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT	2550	0.33181479269382863	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	2299	0.2991538072169067	No Hit
TGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTT	2063	0.2684446734617131	Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	1736	0.22589430592803392	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1612	0.20975899836174577	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	1495	0.19453455493226424	No Hit
TGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	1390	0.1808715928801654	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC	1287	0.16746887772429705	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCCAAGTCGTATGCCGTCTTCTGC	1267	0.1648664087619925	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCCAAGTCGTATGCCGTCTTCTGC	1186	0.15432640946465911	No Hit
CTGTCTCTTATACACATCTGACGCTGTCACAGTCGTATGCCGTCTTCTGCT	1170	0.1522444342948155	No Hit
TTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG	1124	0.14625875568151506	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC	1055	0.1372802377615644	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGT	971	0.12634986811988536	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT	890	0.11580986882255195	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT	884	0.1150291281338606	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATG	774	0.10071554884118564	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	40	6.8102963E-9	45.000004	6
ACGCATT	20	7.031825E-4	45.000004	19
CGCATTG	20	7.031825E-4	45.000004	20
ACTTAGC	20	7.031825E-4	45.000004	11
CTACGCC	20	7.031825E-4	45.000004	15
AACGCAT	20	7.031825E-4	45.000004	18
AAACACG	20	7.031825E-4	45.000004	40
AACGAAC	20	7.031825E-4	45.000004	20
TTCGGTG	40	6.8102963E-9	45.000004	1
CGATGTA	20	7.031825E-4	45.000004	10
AGGCGTA	25	3.8895952E-5	45.0	27
TACGGGT	25	3.8895952E-5	45.0	4
CCGACTC	35	1.2112287E-7	45.0	27
CTTTACG	30	2.1645592E-6	44.999996	1
GATAGTC	30	2.1645592E-6	44.999996	9
TTTCGCG	255	0.0	41.47059	1
TACGGCT	1590	0.0	40.61321	7
GGCATAT	50	1.0804797E-9	40.5	8
CCGTTTG	50	1.0804797E-9	40.5	1
ATACGGC	3445	0.0	40.23222	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content