##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933796.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1208119
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.016055537575355	31.0	16.0	33.0	16.0	34.0
2	28.395146504607577	31.0	25.0	33.0	16.0	34.0
3	29.369855949620856	31.0	28.0	34.0	16.0	34.0
4	32.7199787438158	35.0	32.0	37.0	28.0	37.0
5	33.839898221946676	35.0	33.0	37.0	28.0	37.0
6	33.127179524533595	35.0	33.0	37.0	28.0	37.0
7	33.49726558393668	35.0	32.0	35.0	29.0	37.0
8	33.615292036628844	35.0	35.0	37.0	28.0	37.0
9	34.72752601357979	37.0	32.0	39.0	28.0	39.0
10	33.652303291314844	35.0	32.0	39.0	27.0	39.0
11	34.11799665430309	35.0	32.0	39.0	27.0	39.0
12	34.35368535715438	37.0	32.0	39.0	27.0	39.0
13	34.77582754678968	37.0	34.0	39.0	29.0	39.0
14	34.602049963621134	37.0	33.0	39.0	26.0	40.0
15	35.032232751906065	37.0	33.0	39.0	27.0	40.0
16	34.614795396811076	36.0	32.0	39.0	27.0	40.0
17	34.465419383355446	36.0	32.0	39.0	27.0	40.0
18	34.45210612530719	37.0	32.0	39.0	27.0	40.0
19	34.27262877249675	37.0	32.0	39.0	25.0	40.0
20	35.28651813273361	37.0	34.0	39.0	29.0	40.0
21	34.548892948459546	37.0	32.0	39.0	25.0	40.0
22	35.516102304491525	37.0	34.0	39.0	29.0	40.0
23	36.33853535951342	38.0	35.0	40.0	31.0	40.0
24	36.457454108411504	38.0	35.0	40.0	31.0	41.0
25	34.32382488811119	37.0	32.0	39.0	25.0	40.0
26	35.06985487356792	37.0	33.0	39.0	27.0	40.0
27	35.70257234593612	37.0	34.0	39.0	30.0	40.0
28	35.90460873473557	38.0	34.0	40.0	30.0	41.0
29	36.38113050121718	38.0	35.0	40.0	30.0	41.0
30	34.07889537371732	37.0	32.0	39.0	24.0	40.0
31	34.93214244623253	37.0	33.0	39.0	27.0	40.0
32	35.397481539484104	37.0	34.0	39.0	30.0	40.0
33	36.23939198042577	38.0	35.0	40.0	30.0	40.0
34	36.517215605416354	38.0	35.0	40.0	31.0	41.0
35	36.62884285405659	38.0	35.0	40.0	31.0	41.0
36	36.62255291076458	38.0	35.0	40.0	31.0	41.0
37	36.724960041188	39.0	35.0	40.0	31.0	41.0
38	36.31723447772943	38.0	35.0	40.0	30.0	41.0
39	36.273555833489915	38.0	35.0	40.0	31.0	41.0
40	35.86774067786369	38.0	34.0	40.0	30.0	41.0
41	35.394753331418514	37.0	34.0	40.0	28.0	41.0
42	36.18792354064459	38.0	35.0	40.0	31.0	41.0
43	36.30850272200007	38.0	35.0	40.0	31.0	41.0
44	36.71578048188962	39.0	35.0	40.0	32.0	41.0
45	36.924840185445305	39.0	35.0	40.0	32.0	41.0
46	36.65718774392258	38.0	35.0	40.0	32.0	41.0
47	36.471917915370916	38.0	35.0	40.0	31.0	41.0
48	36.51514875604142	38.0	35.0	40.0	31.0	41.0
49	36.70992261523906	39.0	35.0	40.0	32.0	41.0
50	36.537366766022224	38.0	35.0	40.0	31.0	41.0
51	35.79547544571354	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	11.0
10	18.0
11	20.0
12	39.0
13	46.0
14	59.0
15	83.0
16	143.0
17	247.0
18	516.0
19	1016.0
20	1591.0
21	2094.0
22	3049.0
23	3917.0
24	5429.0
25	7566.0
26	10388.0
27	14047.0
28	18749.0
29	26799.0
30	37210.0
31	52394.0
32	72878.0
33	96949.0
34	124592.0
35	164469.0
36	204776.0
37	215648.0
38	129346.0
39	14027.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.80092358451444	3.9767605674606554	46.49376427322143	17.728551574803475
2	18.09432680058835	13.625147853812415	46.90258161654605	21.377943729053182
3	18.44271963275141	13.492958888983619	48.25261418784077	19.811707290424206
4	18.288595742637938	4.157620234430548	54.670690552834614	22.883093470096902
5	24.670996814055567	5.156031814746727	45.60717942520563	24.565791945992075
6	20.667583243041456	13.301835332446554	48.416091461188834	17.614489963323148
7	74.67103820070705	1.963134426327208	18.466806663913076	4.899020709052667
8	73.54797002613154	9.408344707764716	9.808719174187312	7.2349660919164425
9	68.24782989092962	4.463881455386431	12.050634084887333	15.237654568796616
10	36.85704802258718	24.05905378526453	21.54613908067003	17.53775911147826
11	29.179161986526164	23.805270838385955	26.397482367217133	20.618084807870748
12	24.124030828088955	19.69946669160902	31.599205045198364	24.577297435103663
13	23.297787717931758	21.741897942172915	35.73712523352418	19.223189106371144
14	20.940404049601074	25.221273732140624	28.96287534588894	24.875446872369363
15	16.67186758920272	24.792259702893507	37.15602519288249	21.379847515021286
16	18.8363066883312	23.694106292509264	29.13835474816636	28.331232270993173
17	18.616046929151846	25.344771500158508	32.27438687745165	23.764794693238002
18	18.42939313097468	23.54486602727049	32.678568915810445	25.347171925944384
19	19.73323819921713	25.733806024075445	30.08834394625033	24.4446118304571
20	21.978215722126713	25.183363559384464	34.533104768652755	18.305315949836068
21	23.53907189606322	27.9359069760512	28.55836221431829	19.96665891356729
22	20.11349875302019	23.389748857521486	30.441703176591044	26.055049212867274
23	19.48856031566427	27.804545744252014	28.767447577597906	23.939446362485814
24	22.70620692166914	22.664654723582693	28.14192972712125	26.487208627626913
25	19.56438066117659	28.529226011676002	25.545331213233137	26.361062113914276
26	21.026322738074644	24.23544369387453	28.72423991345223	26.013993654598593
27	20.641095786093917	28.49768938324784	26.62891652229623	24.232298308362008
28	17.21461213671832	25.331279451775863	31.246176908069483	26.20793150343633
29	22.484953882854256	24.68440608913526	26.78651689113407	26.04412313687642
30	19.120798530608326	28.209886608852276	27.80189699855726	24.867417861982137
31	21.256432520306358	28.64303930324745	23.655782253238296	26.444745923207897
32	20.921200643314112	33.89707470870005	22.935240650962363	22.24648399702347
33	18.19042660532613	28.124464560196472	23.517302517384465	30.16780631709294
34	22.51781488412979	27.955027609035206	25.582744746171528	23.94441276066348
35	18.646590277944476	30.258774177047126	24.2869286883163	26.8077068566921
36	22.325366954745352	28.853035172859627	23.52880800649605	25.292789865898975
37	17.077125680500018	32.95734940018326	28.035897126028146	21.929627793288574
38	18.05310569571375	29.13736146853083	23.590225797293147	29.219307038462272
39	20.303049616800994	31.15496072820641	25.956962848858435	22.585026806134163
40	19.966576140264326	27.410958688672228	26.68180866288834	25.940656508175103
41	17.986390413527147	32.791968340867086	24.241734464899565	24.979906780706205
42	18.67961682582593	29.09018068584303	27.928209058875826	24.30199342945521
43	21.974325376887542	27.57062839008409	25.12467728758508	25.330368945443286
44	20.550707339260455	24.59062393688039	28.21435636721217	26.64431235664699
45	20.124507602314008	22.10427946253639	26.580494140064015	31.19071879508558
46	23.539402989275064	27.393576295050405	25.86392565633021	23.20309505934432
47	16.515508819909297	25.551704757561133	33.7648029705683	24.167983451961273
48	18.763052315210672	27.68270344229335	26.641415291043348	26.912828951452628
49	18.753119518855343	24.1307354656288	32.319001687747644	24.797143327768207
50	21.241285005864487	23.63475783428619	27.542568240380294	27.581388919469024
51	18.699565191839547	22.98771892503967	25.79125069633041	32.52146518679037
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	233.0
1	743.0
2	1253.0
3	2769.0
4	4285.0
5	3108.0
6	1931.0
7	1907.0
8	1883.0
9	1853.5
10	1824.0
11	1916.5
12	2009.0
13	1933.5
14	1858.0
15	1998.5
16	2139.0
17	2135.5
18	2132.0
19	2298.5
20	2465.0
21	2762.5
22	3060.0
23	3625.0
24	4190.0
25	5311.5
26	7825.5
27	9218.0
28	11016.5
29	12815.0
30	14845.0
31	16875.0
32	19329.0
33	21783.0
34	24717.0
35	27651.0
36	30548.0
37	33445.0
38	37081.5
39	40718.0
40	46482.5
41	52247.0
42	60095.0
43	67943.0
44	127927.5
45	187912.0
46	167759.0
47	147606.0
48	144026.0
49	140446.0
50	116725.0
51	93004.0
52	81238.5
53	69473.0
54	61745.0
55	54017.0
56	49712.0
57	45407.0
58	42457.5
59	39508.0
60	35940.5
61	32373.0
62	29195.0
63	26017.0
64	22674.5
65	19332.0
66	15700.5
67	12069.0
68	9942.0
69	7815.0
70	6882.5
71	5950.0
72	4912.0
73	3874.0
74	3053.5
75	1817.5
76	1402.0
77	996.5
78	591.0
79	502.0
80	413.0
81	298.0
82	183.0
83	121.5
84	60.0
85	48.0
86	36.0
87	19.0
88	2.0
89	1.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1208119.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.079390199177148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.17320197321165	21.10767123677901
2	12.489351115690468	7.513441310867641
3	4.842245506117818	4.369553760561897
4	2.4463349963039267	2.943370596469136
5	1.4547904112207626	2.187960421856535
6	0.9474225189474873	1.7098734978545267
7	0.6894252139603632	1.451624301670547
8	0.5225989782359658	1.257556686724072
9	0.4377511258948601	1.1850558233328556
>10	5.78524077696892	36.77855900260752
>50	0.13682826759908104	2.7125954204103433
>100	0.0650755877670744	3.7782658685752604
>500	0.0036153104303351963	0.7408971326845863
>1k	0.0036153104303351963	2.334198215019837
>5k	0.0011124032093339066	2.1523408856242976
>10k+	0.0013905040116673833	7.77703583896195
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGC	29161	2.4137522876471604	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG	23315	1.929859558536866	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC	18385	1.5217871749388927	No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	11920	0.9866577712957085	No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	10189	0.8433771838701319	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACACAAAT	8774	0.7262529601802472	No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	6253	0.5175814634154418	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACACAAATCGTA	5549	0.4593090581308629	No Hit
GAACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT	5154	0.42661360346124844	No Hit
TCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	4531	0.3750458357165147	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTC	4370	0.3617193339397857	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCT	4199	0.34756509913344624	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2118	0.17531385567150257	No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG	2055	0.17009913758495646	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGACACAAATCGT	1903	0.15751759553487696	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGACACAAATCGTATG	1701	0.140797388336745	No Hit
TGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCTT	1487	0.12308390150308042	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGACACAAATCGTAT	1252	0.1036321753072338	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6265939034151438	0.0
2	0.0	0.0	0.0	3.004339804274248	0.0
3	0.0	0.0	0.0	3.72860620518343	0.0
4	0.0	0.0	0.0	5.00728818932572	0.0
5	0.0	0.0	0.0	7.943008925445258	0.0
6	0.0	0.0	0.0	8.731755729361097	0.0
7	0.0	0.0	0.0	9.838434789950329	0.0
8	0.0	0.0	0.0	11.30666763787342	0.0
9	0.0	0.0	0.0	11.783607409534987	0.0
10	0.0	0.0	0.0	14.854248629481036	0.0
11	0.0	0.0	0.0	16.81862465535266	0.0
12	8.277330296104937E-5	0.0	0.0	21.045443371058646	0.0
13	8.277330296104937E-5	0.0	0.0	21.800998080487105	0.0
14	8.277330296104937E-5	0.0	0.0	22.090539094244853	0.0
15	8.277330296104937E-5	0.0	0.0	22.93755830344527	0.0
16	8.277330296104937E-5	0.0	0.0	23.804029238841537	0.0
17	8.277330296104937E-5	0.0	0.0	24.886704041572063	0.0
18	8.277330296104937E-5	0.0	0.0	25.919632089222997	0.0
19	8.277330296104937E-5	0.0	0.0	27.475687411587767	0.0
20	8.277330296104937E-5	0.0	0.0	28.397864779876816	0.0
21	8.277330296104937E-5	0.0	0.0	29.5214295942701	0.0
22	8.277330296104937E-5	0.0	0.0	30.788688862603767	0.0
23	8.277330296104937E-5	0.0	0.0	31.956785713990094	0.0
24	8.277330296104937E-5	0.0	0.0	32.89957363471645	0.0
25	8.277330296104937E-5	0.0	0.0	33.73434239507863	0.0
26	8.277330296104937E-5	0.0	0.0	34.46622393986023	0.0
27	8.277330296104937E-5	0.0	0.0	35.18403402313845	0.0
28	8.277330296104937E-5	0.0	0.0	35.89025584400212	0.0
29	8.277330296104937E-5	0.0	0.0	36.65408788372669	0.0
30	8.277330296104937E-5	0.0	0.0	37.46228641383837	0.0
31	1.6554660592209874E-4	0.0	0.0	38.22976047889322	0.0
32	1.6554660592209874E-4	0.0	0.0	38.941528111055284	0.0
33	1.6554660592209874E-4	0.0	0.0	39.65313019661143	0.0
34	1.6554660592209874E-4	0.0	0.0	40.36324236271427	0.0
35	1.6554660592209874E-4	0.0	0.0	41.13617946576455	0.0
36	1.6554660592209874E-4	0.0	0.0	41.856721068040486	0.0
37	1.6554660592209874E-4	0.0	0.0	42.56658491423444	0.0
38	1.6554660592209874E-4	0.0	0.0	43.25989409983619	0.0
39	1.6554660592209874E-4	0.0	0.0	43.95692808407119	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	30	2.1655942E-6	45.000004	22
AGGCACG	70	0.0	45.000004	10
GTCGACG	20	7.0334296E-4	45.0	43
TTCACGA	20	7.0334296E-4	45.0	28
TTAACGC	65	0.0	44.999996	12
TACGGCT	3445	0.0	43.236576	7
ACGGCTG	3525	0.0	42.255318	8
ATACGGC	6750	0.0	41.93333	6
TGATACG	6765	0.0	41.840355	4
GATACGG	6800	0.0	41.625	5
ATGATAC	9520	0.0	41.265755	3
GAATGAT	9845	0.0	41.069073	1
TACGGGA	110	0.0	40.909092	4
AATGATA	9505	0.0	40.83377	2
TTTAGCG	105	0.0	40.714287	1
TATCCGG	50	1.0822987E-9	40.500004	2
TTTGTCG	185	0.0	40.135136	1
TGATACC	2835	0.0	40.07937	4
TACGGCG	3345	0.0	39.41704	7
GATACCT	2895	0.0	39.404144	5
>>END_MODULE