SRR2933794.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933794.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421958
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGC	13838	3.2794733125097757	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG	12049	2.8554974665725026	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	11236	2.6628242621303544	No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	8162	1.9343157375852575	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	6305	1.4942245436749628	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGTGCTTT	4826	1.1437157252617558	No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	4801	1.137790964977557	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	4463	1.0576882059351878	TruSeq Adapter, Index 19 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	3756	0.8901359850980429	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTA	3351	0.7941548684940206	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTC	2891	0.6851392792647609	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT	2805	0.6647581038871168	No Hit
TGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTT	2246	0.5322804639324292	TruSeq Adapter, Index 19 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	1273	0.30168879367140805	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGT	1075	0.25476469222055276	No Hit
TGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	1059	0.25097284563866545	No Hit
TTCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	1022	0.24220420041805107	No Hit
TCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	978	0.23177662231786103	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATG	909	0.21542428393347204	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	902	0.21376535105389638	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTAT	796	0.188644367448893	No Hit
TTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	667	0.15807260438242668	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCATGTGCTT	634	0.15025192080728414	No Hit
TTGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	616	0.14598609340266092	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	597	0.14148327558666976	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC	537	0.1272638509045924	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT	522	0.12370899473407307	No Hit
CGTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	468	0.11091151252020344	No Hit
TGCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	467	0.11067452210883548	No Hit
TTTCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	443	0.10498675223600454	No Hit
TTCCTCGGGCCTTTAAATAGATAAAAGTATCTATCAGATAAAAATCAGGTT	436	0.10332781935642885	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCAGG	30	2.1622236E-6	45.000004	41
CAGGTCG	20	7.028205E-4	45.000004	24
CGTATGG	20	7.028205E-4	45.000004	2
GCGCGCG	20	7.028205E-4	45.000004	1
GAACTCG	20	7.028205E-4	45.000004	9
CGCATAG	20	7.028205E-4	45.000004	18
GTCTCGG	30	2.1622236E-6	45.000004	2
ATTCGTT	20	7.028205E-4	45.000004	15
ATCACAG	30	2.1622236E-6	45.000004	10
TAGTTTG	30	2.1622236E-6	45.000004	1
ACCTCGT	20	7.028205E-4	45.000004	15
CGTTAGG	20	7.028205E-4	45.000004	2
CGTAGAG	20	7.028205E-4	45.000004	38
TATTCTA	35	1.2094824E-7	45.000004	30
GCAATAC	20	7.028205E-4	45.000004	31
GCCACGC	20	7.028205E-4	45.000004	39
TTACACG	80	0.0	45.000004	34
GTCGGTT	20	7.028205E-4	45.000004	27
TCACGGG	25	3.8865954E-5	45.0	3
TCGTTCA	25	3.8865954E-5	45.0	16

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content