##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933794.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421958
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.69504310855583	31.0	16.0	33.0	16.0	34.0
2	27.75661795723745	31.0	25.0	33.0	16.0	34.0
3	28.59112992288332	31.0	28.0	34.0	16.0	34.0
4	32.27163129979761	35.0	28.0	35.0	22.0	37.0
5	33.75837168628157	35.0	33.0	37.0	28.0	37.0
6	32.78889368136165	35.0	32.0	37.0	25.0	37.0
7	33.63521487920599	35.0	32.0	35.0	30.0	37.0
8	33.415584015470735	35.0	33.0	37.0	28.0	37.0
9	34.76003061916115	37.0	32.0	39.0	28.0	39.0
10	34.09850269458098	35.0	32.0	39.0	27.0	39.0
11	34.74270662009015	37.0	34.0	39.0	27.0	39.0
12	34.60160963887401	37.0	33.0	39.0	27.0	39.0
13	35.08027102223444	37.0	34.0	39.0	30.0	39.0
14	34.77607250010664	37.0	33.0	39.0	27.0	40.0
15	35.199590954549976	37.0	33.0	39.0	27.0	40.0
16	34.5192128126496	36.0	32.0	39.0	27.0	40.0
17	34.38096919598633	36.0	32.0	38.0	27.0	40.0
18	34.47583408775281	37.0	32.0	39.0	27.0	40.0
19	34.11329326615445	36.0	32.0	39.0	27.0	40.0
20	35.18758502031008	37.0	33.0	39.0	27.0	40.0
21	34.546104114627525	37.0	32.0	39.0	27.0	40.0
22	35.6373477929083	37.0	34.0	39.0	30.0	40.0
23	36.37193512150498	38.0	35.0	39.0	31.0	40.0
24	36.45949359888899	38.0	35.0	40.0	31.0	40.0
25	34.380146839258884	37.0	32.0	39.0	25.0	40.0
26	35.21262779707933	37.0	34.0	39.0	28.0	40.0
27	35.97941027306035	38.0	34.0	39.0	30.0	40.0
28	36.11288801255101	38.0	35.0	40.0	30.0	40.0
29	36.309293815972204	38.0	35.0	40.0	30.0	41.0
30	34.537243043146475	37.0	33.0	39.0	25.0	40.0
31	34.86308352964039	37.0	33.0	39.0	25.0	40.0
32	35.71021760459572	37.0	34.0	39.0	30.0	40.0
33	36.43927357699108	38.0	35.0	40.0	31.0	40.0
34	36.568691670735	38.0	35.0	40.0	31.0	41.0
35	36.88088861924646	39.0	35.0	40.0	32.0	41.0
36	36.75108423113201	39.0	35.0	40.0	31.0	41.0
37	36.98333957408083	39.0	35.0	40.0	32.0	41.0
38	36.57683229136549	38.0	35.0	40.0	31.0	41.0
39	36.4577967475436	38.0	35.0	40.0	31.0	41.0
40	36.04858066442632	38.0	35.0	40.0	30.0	41.0
41	35.5018532650169	37.0	34.0	40.0	30.0	41.0
42	36.55861720834775	38.0	35.0	40.0	31.0	41.0
43	36.61795960735429	38.0	35.0	40.0	31.0	41.0
44	37.08269780404685	39.0	36.0	40.0	33.0	41.0
45	37.3521013939776	39.0	36.0	40.0	33.0	41.0
46	37.16171751690926	39.0	36.0	40.0	33.0	41.0
47	37.05003578555212	39.0	35.0	40.0	32.0	41.0
48	37.12973329099105	39.0	35.0	40.0	33.0	41.0
49	37.39875769626361	39.0	36.0	40.0	33.0	41.0
50	37.2203655340105	39.0	36.0	40.0	33.0	41.0
51	36.6189668166026	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	11.0
11	1.0
12	5.0
13	6.0
14	13.0
15	13.0
16	25.0
17	59.0
18	127.0
19	216.0
20	400.0
21	546.0
22	759.0
23	1051.0
24	1504.0
25	2192.0
26	3063.0
27	4261.0
28	5835.0
29	8229.0
30	12010.0
31	17011.0
32	24439.0
33	33921.0
34	44747.0
35	60494.0
36	76279.0
37	77034.0
38	43501.0
39	4203.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.816161798093646	3.672877395380583	46.71365396556055	15.797306840965215
2	16.996952303309808	18.780305148853678	44.44565572876921	19.7770868190673
3	17.222330184520736	18.808507007806462	45.24905322330658	18.720109584366217
4	17.929509572042715	3.7987193038169678	56.01339469805051	22.2583764260898
5	27.037762052147368	4.280046829305286	44.94452054469876	23.737670573848582
6	18.991463605382528	16.331009247365852	45.201655141032994	19.475872006218626
7	65.64326307357604	1.406775081880187	26.175590935590748	6.774370908953024
8	61.816341910806294	11.734106238061608	14.36351485218908	12.086036998943023
9	57.379881409998156	4.500447911877485	16.923959256608477	21.195711421515888
10	33.03124955564298	25.50561904265353	24.212599358229966	17.25053204347352
11	24.541779039620057	21.243109503789476	32.17642514183876	22.038686314751704
12	19.152617085112738	17.842534091070675	36.92452803359576	26.080320790220828
13	19.48392968020514	23.733878727266696	39.73973713023571	17.042454462292454
14	18.1411420093943	26.360443456457748	29.796567430881748	25.7018471032662
15	11.927016432915124	26.792476976381536	40.31917868603037	20.961327904672977
16	13.243972148886856	27.124263552296675	28.29120433787249	31.34055996094398
17	13.807535347119856	28.566113215059318	32.6368975111267	24.989453926694125
18	13.493760042468681	24.052156849733862	36.35812095042635	26.095962157371112
19	14.973765161461566	25.844278340498345	31.500528488617352	27.68142800942274
20	16.77536626868077	26.697206831011616	39.05246493726864	17.474961963038975
21	20.007204508505584	29.272344640935827	30.14067750818802	20.579773342370565
22	15.981211400186748	24.10524270188028	31.949388327748256	27.96415757018471
23	16.510410988771397	28.549760876674927	29.34960351504178	25.590224619511893
24	21.20211016262282	22.60485640750975	26.98894202740557	29.204091402461856
25	13.783599315571692	31.500054507794616	25.282848055967655	29.433498120666034
26	15.417174221131013	27.56293280373876	29.680679119722814	27.33921385540741
27	19.179397001597316	25.983154721559963	31.04740282208182	23.7900454547609
28	16.759961891941852	24.18510847051128	34.85654970399897	24.19837993354789
29	18.996440404021254	22.953706293043382	32.94332611302547	25.106527189909894
30	22.4972627607487	23.92702591253158	33.55950118258215	20.01621014413757
31	23.54665630228601	22.770512705055953	26.249057963114815	27.433773029543225
32	19.77921973276961	26.624213784310285	33.667568810165946	19.92899767275416
33	20.552045464240518	18.54236677584025	31.797714464472765	29.107873295446467
34	20.81012802222022	22.56433104716583	31.18177638532745	25.443764545286495
35	14.650984221178412	20.506780295669238	37.60871935121505	27.233516131937304
36	22.530441418340214	23.55566193791799	33.11798804620365	20.795908597538144
37	16.816128619436057	18.70683812132961	44.18709918996677	20.289934069267556
38	25.53761274818821	21.78534356499936	30.79216414903853	21.8848795377739
39	21.62964086473061	19.375388071798618	37.265083254731515	21.72988780873926
40	23.916598334431388	19.028434109555928	33.93133913801847	23.123628417994208
41	17.660762445551452	22.04887690244053	30.71774915986899	29.57261149213903
42	18.175268628631287	17.897278876096674	40.12389858706317	23.803553908208876
43	23.61135468458946	18.914915702510676	32.92886969793202	24.54485991496784
44	19.704567753188705	17.85035477464582	34.93925935756639	27.505818114599084
45	21.02223444039454	16.215831907441025	30.41463842372937	32.34729522843506
46	25.653738049758505	19.59389323107987	33.64742462519966	21.104944093961958
47	15.38494352518497	17.343669275141128	44.36105015191085	22.910337047763047
48	18.942880571052097	19.46615539935254	32.51034463145621	29.080619398139152
49	19.30026211139497	13.921053754165106	43.95200470188976	22.826679432550158
50	23.379104081448865	13.443281084847309	34.93167566440262	28.24593916930121
51	18.486437038757412	14.174870484740188	31.025125723413232	36.31356675308917
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	149.0
1	221.5
2	294.0
3	925.0
4	1556.0
5	1105.5
6	655.0
7	725.5
8	796.0
9	845.5
10	895.0
11	1002.0
12	1109.0
13	1028.5
14	948.0
15	929.0
16	910.0
17	887.5
18	865.0
19	822.0
20	779.0
21	1084.5
22	1390.0
23	1441.5
24	1493.0
25	1714.5
26	2353.0
27	2770.0
28	3231.0
29	3692.0
30	4510.5
31	5329.0
32	5643.5
33	5958.0
34	6988.5
35	8019.0
36	9153.5
37	10288.0
38	11336.0
39	12384.0
40	14556.0
41	16728.0
42	21457.0
43	26186.0
44	60742.0
45	95298.0
46	83170.5
47	71043.0
48	66100.5
49	61158.0
50	45362.0
51	29566.0
52	24188.5
53	18811.0
54	15441.5
55	12072.0
56	10393.5
57	8715.0
58	7535.0
59	6355.0
60	5417.5
61	4480.0
62	3942.5
63	3405.0
64	2856.0
65	2307.0
66	1826.0
67	1345.0
68	1152.0
69	959.0
70	757.5
71	556.0
72	471.5
73	387.0
74	284.5
75	144.5
76	107.0
77	72.5
78	38.0
79	35.5
80	33.0
81	20.5
82	8.0
83	5.0
84	2.0
85	1.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	421958.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.89143817203379
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.33098380318495	21.108423613903305
2	13.41843170476712	8.29029306746943
3	5.697554392354972	5.280169477296994
4	2.960233062223818	3.6578342646598934
5	1.7794894282968488	2.7485493826009932
6	1.1720597304952445	2.172396641911466
7	0.8742394383397106	1.8904559489917303
8	0.722858746481667	1.7864117019241812
9	0.6441296175663411	1.7908281230243759
>10	4.176316718540239	21.715252571267474
>50	0.12187976042882587	2.6855704438863635
>100	0.0809960433229539	4.875489909664153
>500	0.007713908887900372	1.7057065871640278
>1k	0.009256690665480447	7.999044246706073
>5k	0.0015427817775800743	3.447395528988822
>10k+	0.0023141726663701116	8.84617849054068
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGC	13838	3.2794733125097757	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG	12049	2.8554974665725026	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	11236	2.6628242621303544	No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	8162	1.9343157375852575	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	6305	1.4942245436749628	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGTGCTTT	4826	1.1437157252617558	No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	4801	1.137790964977557	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	4463	1.0576882059351878	TruSeq Adapter, Index 19 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	3756	0.8901359850980429	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTA	3351	0.7941548684940206	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTC	2891	0.6851392792647609	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT	2805	0.6647581038871168	No Hit
TGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTT	2246	0.5322804639324292	TruSeq Adapter, Index 19 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	1273	0.30168879367140805	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGT	1075	0.25476469222055276	No Hit
TGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	1059	0.25097284563866545	No Hit
TTCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	1022	0.24220420041805107	No Hit
TCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	978	0.23177662231786103	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATG	909	0.21542428393347204	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	902	0.21376535105389638	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTAT	796	0.188644367448893	No Hit
TTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	667	0.15807260438242668	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCATGTGCTT	634	0.15025192080728414	No Hit
TTGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	616	0.14598609340266092	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	597	0.14148327558666976	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC	537	0.1272638509045924	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT	522	0.12370899473407307	No Hit
CGTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	468	0.11091151252020344	No Hit
TGCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	467	0.11067452210883548	No Hit
TTTCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	443	0.10498675223600454	No Hit
TTCCTCGGGCCTTTAAATAGATAAAAGTATCTATCAGATAAAAATCAGGTT	436	0.10332781935642885	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2908867707212566	0.0
2	0.0	0.0	0.0	6.116722517406946	0.0
3	0.0	0.0	0.0	7.659056114589604	0.0
4	0.0	0.0	0.0	10.513368629105267	0.0
5	0.0	0.0	0.0	15.862479204091402	0.0
6	0.0	0.0	0.0	17.308120713435933	0.0
7	0.0	0.0	0.0	19.42349712530631	0.0
8	0.0	0.0	0.0	22.038686314751704	0.0
9	0.0	0.0	0.0	22.924793462856492	0.0
10	0.0	0.0	0.0	27.51648268311064	0.0
11	0.0	0.0	0.0	30.54853800615227	0.0
12	0.0	0.0	0.0	36.665497513970585	0.0
13	0.0	0.0	0.0	37.79214992961385	0.0
14	0.0	0.0	0.0	38.27561036880448	0.0
15	0.0	0.0	0.0	39.604415605344606	0.0
16	0.0	0.0	0.0	40.84671934173544	0.0
17	0.0	0.0	0.0	42.507074163779336	0.0
18	0.0	0.0	0.0	44.09585788159011	0.0
19	0.0	0.0	0.0	46.561269131050956	0.0
20	0.0	0.0	0.0	47.92775584299859	0.0
21	0.0	0.0	0.0	49.356808023547366	0.0
22	0.0	0.0	0.0	51.00246944008646	0.0
23	0.0	0.0	0.0	52.29454116286455	0.0
24	0.0	0.0	0.0	53.34677858933828	0.0
25	0.0	0.0	0.0	54.21866631276099	0.0
26	0.0	0.0	0.0	55.065196062167324	0.0
27	0.0	0.0	0.0	55.845368496390634	0.0
28	0.0	0.0	0.0	56.653268808743995	0.0
29	0.0	0.0	0.0	57.522312647230294	0.0
30	0.0	0.0	0.0	58.460794676247396	0.0
31	0.0	0.0	0.0	59.255897506386894	0.0
32	0.0	0.0	0.0	60.04246868171714	0.0
33	0.0	0.0	0.0	60.781641774773796	0.0
34	0.0	0.0	0.0	61.49427194175723	0.0
35	0.0	2.3699041136795605E-4	0.0	62.199318415576904	0.0
36	0.0	2.3699041136795605E-4	0.0	62.92403509354011	0.0
37	0.0	2.3699041136795605E-4	0.0	63.618890979670965	0.0
38	0.0	2.3699041136795605E-4	0.0	64.29952744111974	0.0
39	0.0	2.3699041136795605E-4	0.0	65.0038629437053	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCAGG	30	2.1622236E-6	45.000004	41
CAGGTCG	20	7.028205E-4	45.000004	24
CGTATGG	20	7.028205E-4	45.000004	2
GCGCGCG	20	7.028205E-4	45.000004	1
GAACTCG	20	7.028205E-4	45.000004	9
CGCATAG	20	7.028205E-4	45.000004	18
GTCTCGG	30	2.1622236E-6	45.000004	2
ATTCGTT	20	7.028205E-4	45.000004	15
ATCACAG	30	2.1622236E-6	45.000004	10
TAGTTTG	30	2.1622236E-6	45.000004	1
ACCTCGT	20	7.028205E-4	45.000004	15
CGTTAGG	20	7.028205E-4	45.000004	2
CGTAGAG	20	7.028205E-4	45.000004	38
TATTCTA	35	1.2094824E-7	45.000004	30
GCAATAC	20	7.028205E-4	45.000004	31
GCCACGC	20	7.028205E-4	45.000004	39
TTACACG	80	0.0	45.000004	34
GTCGGTT	20	7.028205E-4	45.000004	27
TCACGGG	25	3.8865954E-5	45.0	3
TCGTTCA	25	3.8865954E-5	45.0	16
>>END_MODULE