FastQCFastQC Report
Sat 14 Jan 2017
SRR2933793.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933793.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528580
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGC150452.846305195050891No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCG115402.183207839872867No Hit
GAATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC103161.9516440273941504No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC56341.0658746074387981TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGCTGTGT46910.8874720950471074No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC39110.7399069204283174TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT34660.6557190964470846No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTA32040.6061523326648757No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT31410.5942336070225888TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC25670.48564077339286393TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTC24090.45574936622649365No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCT22830.4319119149419199No Hit
TGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCTT13670.25861742782549474TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGT11160.21113171137765335No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10120.1914563547618147No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATG9850.18634832948654886No Hit
CGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG7720.14605168564834084No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTAT6920.13091679594384956No Hit
TCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG6610.1250520261833592No Hit
TGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG5740.10859283362972492No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCATGCTGTG5300.10026864429225472No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGC207.029826E-445.018
GAGTACA351.2102646E-745.025
TTCCGAT253.8879363E-545.013
TCTAGTC207.029826E-445.013
ATTCAAG253.8879363E-545.041
GCGATTA207.029826E-445.09
TGTAGCG207.029826E-445.01
TTACGAG207.029826E-445.012
CCGCGAA207.029826E-445.015
CACAACG207.029826E-445.012
TTACCGG207.029826E-445.02
TCGTTCA1100.042.95454416
CGTTCAT1100.042.95454417
TACGGCT17700.042.2033927
ACGGCTG17850.041.848748
TTTCGCG1250.041.41
TTACGGG1250.041.43
ATACGGC35350.041.3719946
TCGTTTG1200.041.2500041
CTACGGC603.6379788E-1241.2500046
Produced by FastQC (version 0.11.5)