##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933793.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	528580
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.92164100041621	31.0	16.0	33.0	16.0	34.0
2	28.161616027848197	31.0	25.0	33.0	16.0	34.0
3	29.10015513261947	31.0	28.0	34.0	16.0	34.0
4	32.537657497445984	35.0	32.0	37.0	26.0	37.0
5	33.79311362518445	35.0	33.0	37.0	28.0	37.0
6	32.945094403874535	35.0	33.0	37.0	26.0	37.0
7	33.47462068182678	35.0	32.0	35.0	29.0	37.0
8	33.5087044534413	35.0	33.0	37.0	28.0	37.0
9	34.72067804305876	37.0	32.0	39.0	28.0	39.0
10	33.6376745241969	35.0	32.0	39.0	27.0	39.0
11	34.02511824132581	35.0	32.0	39.0	27.0	39.0
12	34.2311267925385	37.0	32.0	39.0	27.0	39.0
13	34.61462976276061	37.0	33.0	39.0	27.0	39.0
14	34.44756517461879	37.0	32.0	39.0	25.0	40.0
15	34.80119565628665	37.0	32.0	39.0	27.0	40.0
16	34.51157629876272	36.0	32.0	39.0	27.0	40.0
17	34.41021983427296	36.0	32.0	38.0	27.0	40.0
18	34.27455257482311	36.0	32.0	39.0	27.0	40.0
19	33.99272390177457	36.0	32.0	39.0	25.0	40.0
20	34.99221120738583	37.0	33.0	39.0	27.0	40.0
21	34.415112187369935	37.0	32.0	39.0	25.0	40.0
22	35.38351242952817	37.0	34.0	39.0	28.0	40.0
23	36.16034469711302	38.0	34.0	39.0	30.0	40.0
24	36.278996556812594	38.0	35.0	40.0	31.0	40.0
25	34.172681524083394	37.0	32.0	39.0	24.0	40.0
26	34.932401906996105	37.0	33.0	39.0	27.0	40.0
27	35.6286352113209	37.0	34.0	39.0	30.0	40.0
28	35.75222293692535	38.0	34.0	40.0	30.0	40.0
29	36.2690037458852	38.0	35.0	40.0	30.0	41.0
30	34.34838245866283	37.0	33.0	39.0	24.0	40.0
31	34.97836278330622	37.0	33.0	39.0	27.0	40.0
32	35.465950281887324	37.0	34.0	39.0	30.0	40.0
33	36.23310946308979	38.0	35.0	40.0	30.0	40.0
34	36.45968822127209	38.0	35.0	40.0	31.0	41.0
35	36.63546861402248	38.0	35.0	40.0	31.0	41.0
36	36.51046577623065	38.0	35.0	40.0	31.0	41.0
37	36.81483975935525	39.0	35.0	40.0	32.0	41.0
38	36.28006735025919	38.0	35.0	40.0	30.0	41.0
39	36.27402663740588	38.0	35.0	40.0	31.0	41.0
40	35.897007075560936	38.0	34.0	40.0	30.0	41.0
41	35.50114835975633	37.0	34.0	40.0	29.0	41.0
42	36.30424533656211	38.0	35.0	40.0	31.0	41.0
43	36.32062885466722	38.0	35.0	40.0	31.0	41.0
44	36.76260736312384	39.0	35.0	40.0	32.0	41.0
45	37.0429603844262	39.0	35.0	40.0	33.0	41.0
46	36.81572704226418	39.0	35.0	40.0	32.0	41.0
47	36.63312081425707	39.0	35.0	40.0	31.0	41.0
48	36.70381210034432	38.0	35.0	40.0	32.0	41.0
49	36.87603957773658	39.0	35.0	40.0	32.0	41.0
50	36.72054372091264	39.0	35.0	40.0	32.0	41.0
51	35.99464792462825	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	6.0
11	12.0
12	11.0
13	16.0
14	23.0
15	32.0
16	60.0
17	134.0
18	282.0
19	490.0
20	721.0
21	1083.0
22	1365.0
23	1793.0
24	2320.0
25	3354.0
26	4466.0
27	6112.0
28	8475.0
29	11852.0
30	16740.0
31	23446.0
32	32903.0
33	43437.0
34	54367.0
35	71228.0
36	89454.0
37	92901.0
38	55767.0
39	5726.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.52941844186311	3.8845586287789926	46.84872677740361	16.737296151954293
2	18.27329827083885	15.743879828975746	46.280979227363886	19.70184267282152
3	18.397025994173067	15.586666162170342	46.7743766317303	19.241931211926293
4	17.971168035112946	3.9628816829997353	55.03745885201862	23.028491429868705
5	25.425290400696205	4.959703356161792	44.93794695221159	24.677059290930416
6	19.45136024821219	14.83862423852586	47.54341821483976	18.16659729842219
7	72.96151954292634	1.8394566574596087	20.113511672783684	5.085512126830376
8	71.21646675999848	10.61334140527451	10.437209126338491	7.732982708388512
9	66.79348442998221	4.282417041885807	12.06685837526959	16.857240152862385
10	37.21366680540316	26.12319800219456	19.588709372280448	17.074425820121835
11	30.841689053691024	22.997843278217108	25.931552461311437	20.22891520678043
12	25.020621287222372	18.763290325021757	31.236331302735632	24.979757085020243
13	23.26762268719967	21.80861931968671	36.11336032388664	18.810397669226987
14	20.697718415377047	25.62601687540202	29.099474062582768	24.576790646638162
15	16.546028983313786	23.34008097165992	37.50917552688335	22.60471451814295
16	19.044231715161374	23.598698399485414	28.985773203677777	28.371296681675435
17	19.271255060728745	24.115554882893793	31.96640054485603	24.646789511521437
18	18.822127208747965	22.86408869045367	32.173937719928865	26.139846380869496
19	19.757085020242915	25.06091793106058	29.293011464678955	25.888985584017554
20	21.187142911196037	25.375534450792692	35.50304589655303	17.93427674145825
21	23.749479738166407	27.603390215293803	28.098679480873283	20.548450565666503
22	20.24915812176019	22.569147527337392	30.994362253585077	26.187332097317338
23	20.20772636119414	27.611525218509968	28.778992773090167	23.40175564720572
24	23.03322108290136	23.06273411782512	26.682242990654203	27.22180180861932
25	18.748155435317265	29.730031404896135	24.723031518407808	26.79878164137879
26	18.575050134322147	25.604260471451813	29.022853683453782	26.797835710772254
27	21.09122554769382	26.020091566082716	28.426539029096826	24.46214385712664
28	17.918574293389835	25.70074539331795	31.83510537666957	24.54557493662265
29	20.557531499489198	23.738696129251956	28.316811078739264	27.38696129251958
30	21.057739604222633	23.92920655340724	33.49483521888834	21.51821862348178
31	27.05853418593212	23.521132089749898	24.667032426501194	24.753301297816794
32	22.079155473154486	27.339097203829127	27.55268833478376	23.029058988232624
33	23.172083695940067	20.68769155094782	28.622157478527377	27.518067274584734
34	25.970146430057888	23.984638086949943	27.883196488705586	22.162018994286576
35	19.683113246812216	21.585001324302848	32.06364221120739	26.66824321767755
36	25.816716485678608	26.523326648757045	27.152559688221274	20.50739717734307
37	20.94082258125544	22.01445381966779	34.19331037875061	22.851413220326155
38	24.421658027167126	22.547201937265882	28.41802565363805	24.61311438192894
39	25.584017556472055	21.16425139051799	28.59850921336411	24.653221839645845
40	21.511218736993452	20.58742290665557	36.1120360210375	21.789322335313482
41	26.2168451322411	23.293541185818608	27.21877483067842	23.27083885126187
42	22.007264747058155	20.54031556245034	33.68231866510273	23.770101025388776
43	27.1996670324265	20.800446479246283	26.80710583071626	25.192780657610957
44	22.52676983616482	19.719815354345606	31.672216126224978	26.081198683264596
45	22.04472359907677	18.921260736312384	28.64202202126452	30.391993643346325
46	26.389572060993604	21.75016080820311	28.823829883839718	23.036437246963562
47	17.593174164743274	20.67634038366945	39.07469068069167	22.655794770895607
48	22.225017972681524	22.06042604714518	29.494116311627376	26.220439668545914
49	21.26130387074804	17.90268265920012	36.94275227969276	23.893261190359073
50	24.638654508305272	17.700442695523858	30.915471641000416	26.745431155170458
51	20.850959173635022	18.19289416928374	28.774641492300123	32.18150516478111
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	83.0
1	316.0
2	549.0
3	1291.0
4	2033.0
5	1455.0
6	877.0
7	882.0
8	887.0
9	968.0
10	1049.0
11	1001.0
12	953.0
13	1001.0
14	1049.0
15	1028.5
16	1008.0
17	998.5
18	989.0
19	964.0
20	939.0
21	1059.0
22	1179.0
23	1273.5
24	1368.0
25	1738.0
26	2658.0
27	3208.0
28	3626.0
29	4044.0
30	5086.0
31	6128.0
32	6839.0
33	7550.0
34	8895.5
35	10241.0
36	11353.0
37	12465.0
38	13937.0
39	15409.0
40	16682.0
41	17955.0
42	21309.0
43	24663.0
44	28080.0
45	31497.0
46	63256.0
47	95015.0
48	82516.5
49	70018.0
50	65876.5
51	61735.0
52	49782.0
53	37829.0
54	33058.0
55	28287.0
56	24579.5
57	20872.0
58	19185.0
59	17498.0
60	16566.5
61	15635.0
62	13356.0
63	11077.0
64	9214.0
65	7351.0
66	6138.5
67	4926.0
68	4196.0
69	3466.0
70	2936.0
71	2406.0
72	2076.5
73	1747.0
74	1519.0
75	908.0
76	525.0
77	451.5
78	378.0
79	281.5
80	185.0
81	137.5
82	90.0
83	51.5
84	13.0
85	7.5
86	2.0
87	2.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	528580.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.013705129500536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.40119589032668	21.83401930106796
2	12.439745989799862	7.716052280270794
3	4.865426967251625	4.526847518743859
4	2.413877398461806	2.9945312741864125
5	1.4450817081516554	2.2408668992325196
6	0.9399790009188003	1.749133893745091
7	0.6936947625496562	1.505983137091575
8	0.5546033514025684	1.376024384338562
9	0.4555051644425641	1.2714212569487704
>10	5.605418342830423	34.67932809469277
>50	0.1152264327661943	2.409981393437374
>100	0.057305124316877384	3.188146823147705
>500	0.00369710479463725	0.8053018469546502
>1k	0.006778025456834959	5.573858322289103
>5k	6.161841324395417E-4	1.0766660193978403
>10k+	0.001848552397318625	7.051837554455041
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGC	15045	2.846305195050891	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCG	11540	2.183207839872867	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC	10316	1.9516440273941504	No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	5634	1.0658746074387981	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGCTGTGT	4691	0.8874720950471074	No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	3911	0.7399069204283174	TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT	3466	0.6557190964470846	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTA	3204	0.6061523326648757	No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT	3141	0.5942336070225888	TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	2567	0.48564077339286393	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTC	2409	0.45574936622649365	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCT	2283	0.4319119149419199	No Hit
TGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCTT	1367	0.25861742782549474	TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGT	1116	0.21113171137765335	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1012	0.1914563547618147	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATG	985	0.18634832948654886	No Hit
CGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG	772	0.14605168564834084	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTAT	692	0.13091679594384956	No Hit
TCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG	661	0.1250520261833592	No Hit
TGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG	574	0.10859283362972492	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCATGCTGTG	530	0.10026864429225472	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6725566612433312	0.0
2	0.0	0.0	0.0	3.120057512580877	0.0
3	0.0	0.0	0.0	3.908585266184873	0.0
4	0.0	0.0	0.0	5.602746982481365	0.0
5	0.0	0.0	0.0	9.102690226644974	0.0
6	0.0	0.0	0.0	9.973324756895835	0.0
7	0.0	0.0	0.0	11.204737220477506	0.0
8	0.0	0.0	0.0	12.840062053047788	0.0
9	0.0	0.0	0.0	13.35616179197094	0.0
10	0.0	0.0	0.0	16.753187786144007	0.0
11	0.0	0.0	0.0	18.77293881720837	0.0
12	0.0	0.0	0.0	23.66358923909342	0.0
13	0.0	0.0	0.0	24.51454841272844	0.0
14	0.0	0.0	0.0	24.858677967384313	0.0
15	0.0	0.0	0.0	25.867607552309963	0.0
16	0.0	0.0	0.0	26.802943736047524	0.0
17	0.0	0.0	0.0	27.895493586590487	0.0
18	0.0	0.0	0.0	29.00885391047713	0.0
19	0.0	0.0	0.0	30.781906239358282	0.0
20	0.0	0.0	0.0	31.76340383669454	0.0
21	0.0	0.0	0.0	32.92103371296682	0.0
22	0.0	0.0	0.0	34.14904082636498	0.0
23	1.8918612130614098E-4	0.0	0.0	35.241969049150555	0.0
24	1.8918612130614098E-4	0.0	0.0	36.12679253849937	0.0
25	1.8918612130614098E-4	0.0	0.0	36.89129365469749	0.0
26	1.8918612130614098E-4	0.0	0.0	37.60906579893299	0.0
27	1.8918612130614098E-4	0.0	0.0	38.281054901812404	0.0
28	1.8918612130614098E-4	0.0	0.0	38.96817889439631	0.0
29	1.8918612130614098E-4	0.0	0.0	39.67724847705173	0.0
30	1.8918612130614098E-4	0.0	0.0	40.479208445268455	0.0
31	1.8918612130614098E-4	0.0	0.0	41.17427825494722	0.0
32	1.8918612130614098E-4	0.0	0.0	41.86405085322941	0.0
33	1.8918612130614098E-4	0.0	0.0	42.521661810889555	0.0
34	1.8918612130614098E-4	0.0	0.0	43.22638011275493	0.0
35	1.8918612130614098E-4	0.0	0.0	43.911990616368385	0.0
36	1.8918612130614098E-4	0.0	0.0	44.583033788641266	0.0
37	1.8918612130614098E-4	0.0	0.0	45.23610427939006	0.0
38	1.8918612130614098E-4	0.0	0.0	45.89522872602066	0.0
39	1.8918612130614098E-4	0.0	0.0	46.619054826137955	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCGC	20	7.029826E-4	45.0	18
GAGTACA	35	1.2102646E-7	45.0	25
TTCCGAT	25	3.8879363E-5	45.0	13
TCTAGTC	20	7.029826E-4	45.0	13
ATTCAAG	25	3.8879363E-5	45.0	41
GCGATTA	20	7.029826E-4	45.0	9
TGTAGCG	20	7.029826E-4	45.0	1
TTACGAG	20	7.029826E-4	45.0	12
CCGCGAA	20	7.029826E-4	45.0	15
CACAACG	20	7.029826E-4	45.0	12
TTACCGG	20	7.029826E-4	45.0	2
TCGTTCA	110	0.0	42.954544	16
CGTTCAT	110	0.0	42.954544	17
TACGGCT	1770	0.0	42.203392	7
ACGGCTG	1785	0.0	41.84874	8
TTTCGCG	125	0.0	41.4	1
TTACGGG	125	0.0	41.4	3
ATACGGC	3535	0.0	41.371994	6
TCGTTTG	120	0.0	41.250004	1
CTACGGC	60	3.6379788E-12	41.250004	6
>>END_MODULE