##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933793.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 528580 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.92164100041621 31.0 16.0 33.0 16.0 34.0 2 28.161616027848197 31.0 25.0 33.0 16.0 34.0 3 29.10015513261947 31.0 28.0 34.0 16.0 34.0 4 32.537657497445984 35.0 32.0 37.0 26.0 37.0 5 33.79311362518445 35.0 33.0 37.0 28.0 37.0 6 32.945094403874535 35.0 33.0 37.0 26.0 37.0 7 33.47462068182678 35.0 32.0 35.0 29.0 37.0 8 33.5087044534413 35.0 33.0 37.0 28.0 37.0 9 34.72067804305876 37.0 32.0 39.0 28.0 39.0 10 33.6376745241969 35.0 32.0 39.0 27.0 39.0 11 34.02511824132581 35.0 32.0 39.0 27.0 39.0 12 34.2311267925385 37.0 32.0 39.0 27.0 39.0 13 34.61462976276061 37.0 33.0 39.0 27.0 39.0 14 34.44756517461879 37.0 32.0 39.0 25.0 40.0 15 34.80119565628665 37.0 32.0 39.0 27.0 40.0 16 34.51157629876272 36.0 32.0 39.0 27.0 40.0 17 34.41021983427296 36.0 32.0 38.0 27.0 40.0 18 34.27455257482311 36.0 32.0 39.0 27.0 40.0 19 33.99272390177457 36.0 32.0 39.0 25.0 40.0 20 34.99221120738583 37.0 33.0 39.0 27.0 40.0 21 34.415112187369935 37.0 32.0 39.0 25.0 40.0 22 35.38351242952817 37.0 34.0 39.0 28.0 40.0 23 36.16034469711302 38.0 34.0 39.0 30.0 40.0 24 36.278996556812594 38.0 35.0 40.0 31.0 40.0 25 34.172681524083394 37.0 32.0 39.0 24.0 40.0 26 34.932401906996105 37.0 33.0 39.0 27.0 40.0 27 35.6286352113209 37.0 34.0 39.0 30.0 40.0 28 35.75222293692535 38.0 34.0 40.0 30.0 40.0 29 36.2690037458852 38.0 35.0 40.0 30.0 41.0 30 34.34838245866283 37.0 33.0 39.0 24.0 40.0 31 34.97836278330622 37.0 33.0 39.0 27.0 40.0 32 35.465950281887324 37.0 34.0 39.0 30.0 40.0 33 36.23310946308979 38.0 35.0 40.0 30.0 40.0 34 36.45968822127209 38.0 35.0 40.0 31.0 41.0 35 36.63546861402248 38.0 35.0 40.0 31.0 41.0 36 36.51046577623065 38.0 35.0 40.0 31.0 41.0 37 36.81483975935525 39.0 35.0 40.0 32.0 41.0 38 36.28006735025919 38.0 35.0 40.0 30.0 41.0 39 36.27402663740588 38.0 35.0 40.0 31.0 41.0 40 35.897007075560936 38.0 34.0 40.0 30.0 41.0 41 35.50114835975633 37.0 34.0 40.0 29.0 41.0 42 36.30424533656211 38.0 35.0 40.0 31.0 41.0 43 36.32062885466722 38.0 35.0 40.0 31.0 41.0 44 36.76260736312384 39.0 35.0 40.0 32.0 41.0 45 37.0429603844262 39.0 35.0 40.0 33.0 41.0 46 36.81572704226418 39.0 35.0 40.0 32.0 41.0 47 36.63312081425707 39.0 35.0 40.0 31.0 41.0 48 36.70381210034432 38.0 35.0 40.0 32.0 41.0 49 36.87603957773658 39.0 35.0 40.0 32.0 41.0 50 36.72054372091264 39.0 35.0 40.0 32.0 41.0 51 35.99464792462825 38.0 34.0 40.0 30.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 3.0 10 6.0 11 12.0 12 11.0 13 16.0 14 23.0 15 32.0 16 60.0 17 134.0 18 282.0 19 490.0 20 721.0 21 1083.0 22 1365.0 23 1793.0 24 2320.0 25 3354.0 26 4466.0 27 6112.0 28 8475.0 29 11852.0 30 16740.0 31 23446.0 32 32903.0 33 43437.0 34 54367.0 35 71228.0 36 89454.0 37 92901.0 38 55767.0 39 5726.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.52941844186311 3.8845586287789926 46.84872677740361 16.737296151954293 2 18.27329827083885 15.743879828975746 46.280979227363886 19.70184267282152 3 18.397025994173067 15.586666162170342 46.7743766317303 19.241931211926293 4 17.971168035112946 3.9628816829997353 55.03745885201862 23.028491429868705 5 25.425290400696205 4.959703356161792 44.93794695221159 24.677059290930416 6 19.45136024821219 14.83862423852586 47.54341821483976 18.16659729842219 7 72.96151954292634 1.8394566574596087 20.113511672783684 5.085512126830376 8 71.21646675999848 10.61334140527451 10.437209126338491 7.732982708388512 9 66.79348442998221 4.282417041885807 12.06685837526959 16.857240152862385 10 37.21366680540316 26.12319800219456 19.588709372280448 17.074425820121835 11 30.841689053691024 22.997843278217108 25.931552461311437 20.22891520678043 12 25.020621287222372 18.763290325021757 31.236331302735632 24.979757085020243 13 23.26762268719967 21.80861931968671 36.11336032388664 18.810397669226987 14 20.697718415377047 25.62601687540202 29.099474062582768 24.576790646638162 15 16.546028983313786 23.34008097165992 37.50917552688335 22.60471451814295 16 19.044231715161374 23.598698399485414 28.985773203677777 28.371296681675435 17 19.271255060728745 24.115554882893793 31.96640054485603 24.646789511521437 18 18.822127208747965 22.86408869045367 32.173937719928865 26.139846380869496 19 19.757085020242915 25.06091793106058 29.293011464678955 25.888985584017554 20 21.187142911196037 25.375534450792692 35.50304589655303 17.93427674145825 21 23.749479738166407 27.603390215293803 28.098679480873283 20.548450565666503 22 20.24915812176019 22.569147527337392 30.994362253585077 26.187332097317338 23 20.20772636119414 27.611525218509968 28.778992773090167 23.40175564720572 24 23.03322108290136 23.06273411782512 26.682242990654203 27.22180180861932 25 18.748155435317265 29.730031404896135 24.723031518407808 26.79878164137879 26 18.575050134322147 25.604260471451813 29.022853683453782 26.797835710772254 27 21.09122554769382 26.020091566082716 28.426539029096826 24.46214385712664 28 17.918574293389835 25.70074539331795 31.83510537666957 24.54557493662265 29 20.557531499489198 23.738696129251956 28.316811078739264 27.38696129251958 30 21.057739604222633 23.92920655340724 33.49483521888834 21.51821862348178 31 27.05853418593212 23.521132089749898 24.667032426501194 24.753301297816794 32 22.079155473154486 27.339097203829127 27.55268833478376 23.029058988232624 33 23.172083695940067 20.68769155094782 28.622157478527377 27.518067274584734 34 25.970146430057888 23.984638086949943 27.883196488705586 22.162018994286576 35 19.683113246812216 21.585001324302848 32.06364221120739 26.66824321767755 36 25.816716485678608 26.523326648757045 27.152559688221274 20.50739717734307 37 20.94082258125544 22.01445381966779 34.19331037875061 22.851413220326155 38 24.421658027167126 22.547201937265882 28.41802565363805 24.61311438192894 39 25.584017556472055 21.16425139051799 28.59850921336411 24.653221839645845 40 21.511218736993452 20.58742290665557 36.1120360210375 21.789322335313482 41 26.2168451322411 23.293541185818608 27.21877483067842 23.27083885126187 42 22.007264747058155 20.54031556245034 33.68231866510273 23.770101025388776 43 27.1996670324265 20.800446479246283 26.80710583071626 25.192780657610957 44 22.52676983616482 19.719815354345606 31.672216126224978 26.081198683264596 45 22.04472359907677 18.921260736312384 28.64202202126452 30.391993643346325 46 26.389572060993604 21.75016080820311 28.823829883839718 23.036437246963562 47 17.593174164743274 20.67634038366945 39.07469068069167 22.655794770895607 48 22.225017972681524 22.06042604714518 29.494116311627376 26.220439668545914 49 21.26130387074804 17.90268265920012 36.94275227969276 23.893261190359073 50 24.638654508305272 17.700442695523858 30.915471641000416 26.745431155170458 51 20.850959173635022 18.19289416928374 28.774641492300123 32.18150516478111 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 83.0 1 316.0 2 549.0 3 1291.0 4 2033.0 5 1455.0 6 877.0 7 882.0 8 887.0 9 968.0 10 1049.0 11 1001.0 12 953.0 13 1001.0 14 1049.0 15 1028.5 16 1008.0 17 998.5 18 989.0 19 964.0 20 939.0 21 1059.0 22 1179.0 23 1273.5 24 1368.0 25 1738.0 26 2658.0 27 3208.0 28 3626.0 29 4044.0 30 5086.0 31 6128.0 32 6839.0 33 7550.0 34 8895.5 35 10241.0 36 11353.0 37 12465.0 38 13937.0 39 15409.0 40 16682.0 41 17955.0 42 21309.0 43 24663.0 44 28080.0 45 31497.0 46 63256.0 47 95015.0 48 82516.5 49 70018.0 50 65876.5 51 61735.0 52 49782.0 53 37829.0 54 33058.0 55 28287.0 56 24579.5 57 20872.0 58 19185.0 59 17498.0 60 16566.5 61 15635.0 62 13356.0 63 11077.0 64 9214.0 65 7351.0 66 6138.5 67 4926.0 68 4196.0 69 3466.0 70 2936.0 71 2406.0 72 2076.5 73 1747.0 74 1519.0 75 908.0 76 525.0 77 451.5 78 378.0 79 281.5 80 185.0 81 137.5 82 90.0 83 51.5 84 13.0 85 7.5 86 2.0 87 2.0 88 2.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 528580.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.013705129500536 #Duplication Level Percentage of deduplicated Percentage of total 1 70.40119589032668 21.83401930106796 2 12.439745989799862 7.716052280270794 3 4.865426967251625 4.526847518743859 4 2.413877398461806 2.9945312741864125 5 1.4450817081516554 2.2408668992325196 6 0.9399790009188003 1.749133893745091 7 0.6936947625496562 1.505983137091575 8 0.5546033514025684 1.376024384338562 9 0.4555051644425641 1.2714212569487704 >10 5.605418342830423 34.67932809469277 >50 0.1152264327661943 2.409981393437374 >100 0.057305124316877384 3.188146823147705 >500 0.00369710479463725 0.8053018469546502 >1k 0.006778025456834959 5.573858322289103 >5k 6.161841324395417E-4 1.0766660193978403 >10k+ 0.001848552397318625 7.051837554455041 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGC 15045 2.846305195050891 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCG 11540 2.183207839872867 No Hit GAATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC 10316 1.9516440273941504 No Hit GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC 5634 1.0658746074387981 TruSeq Adapter, Index 14 (95% over 21bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGCTGTGT 4691 0.8874720950471074 No Hit CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC 3911 0.7399069204283174 TruSeq Adapter, Index 14 (95% over 21bp) GAACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT 3466 0.6557190964470846 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTA 3204 0.6061523326648757 No Hit CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT 3141 0.5942336070225888 TruSeq Adapter, Index 15 (95% over 22bp) TCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC 2567 0.48564077339286393 TruSeq Adapter, Index 14 (95% over 21bp) GAATGATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTC 2409 0.45574936622649365 No Hit GAATGACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCT 2283 0.4319119149419199 No Hit TGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCTT 1367 0.25861742782549474 TruSeq Adapter, Index 14 (95% over 23bp) GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGT 1116 0.21113171137765335 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1012 0.1914563547618147 No Hit GAATGATACGGCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATG 985 0.18634832948654886 No Hit CGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG 772 0.14605168564834084 No Hit GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTAT 692 0.13091679594384956 No Hit TCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG 661 0.1250520261833592 No Hit TGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG 574 0.10859283362972492 No Hit GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCATGCTGTG 530 0.10026864429225472 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.6725566612433312 0.0 2 0.0 0.0 0.0 3.120057512580877 0.0 3 0.0 0.0 0.0 3.908585266184873 0.0 4 0.0 0.0 0.0 5.602746982481365 0.0 5 0.0 0.0 0.0 9.102690226644974 0.0 6 0.0 0.0 0.0 9.973324756895835 0.0 7 0.0 0.0 0.0 11.204737220477506 0.0 8 0.0 0.0 0.0 12.840062053047788 0.0 9 0.0 0.0 0.0 13.35616179197094 0.0 10 0.0 0.0 0.0 16.753187786144007 0.0 11 0.0 0.0 0.0 18.77293881720837 0.0 12 0.0 0.0 0.0 23.66358923909342 0.0 13 0.0 0.0 0.0 24.51454841272844 0.0 14 0.0 0.0 0.0 24.858677967384313 0.0 15 0.0 0.0 0.0 25.867607552309963 0.0 16 0.0 0.0 0.0 26.802943736047524 0.0 17 0.0 0.0 0.0 27.895493586590487 0.0 18 0.0 0.0 0.0 29.00885391047713 0.0 19 0.0 0.0 0.0 30.781906239358282 0.0 20 0.0 0.0 0.0 31.76340383669454 0.0 21 0.0 0.0 0.0 32.92103371296682 0.0 22 0.0 0.0 0.0 34.14904082636498 0.0 23 1.8918612130614098E-4 0.0 0.0 35.241969049150555 0.0 24 1.8918612130614098E-4 0.0 0.0 36.12679253849937 0.0 25 1.8918612130614098E-4 0.0 0.0 36.89129365469749 0.0 26 1.8918612130614098E-4 0.0 0.0 37.60906579893299 0.0 27 1.8918612130614098E-4 0.0 0.0 38.281054901812404 0.0 28 1.8918612130614098E-4 0.0 0.0 38.96817889439631 0.0 29 1.8918612130614098E-4 0.0 0.0 39.67724847705173 0.0 30 1.8918612130614098E-4 0.0 0.0 40.479208445268455 0.0 31 1.8918612130614098E-4 0.0 0.0 41.17427825494722 0.0 32 1.8918612130614098E-4 0.0 0.0 41.86405085322941 0.0 33 1.8918612130614098E-4 0.0 0.0 42.521661810889555 0.0 34 1.8918612130614098E-4 0.0 0.0 43.22638011275493 0.0 35 1.8918612130614098E-4 0.0 0.0 43.911990616368385 0.0 36 1.8918612130614098E-4 0.0 0.0 44.583033788641266 0.0 37 1.8918612130614098E-4 0.0 0.0 45.23610427939006 0.0 38 1.8918612130614098E-4 0.0 0.0 45.89522872602066 0.0 39 1.8918612130614098E-4 0.0 0.0 46.619054826137955 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGCGC 20 7.029826E-4 45.0 18 GAGTACA 35 1.2102646E-7 45.0 25 TTCCGAT 25 3.8879363E-5 45.0 13 TCTAGTC 20 7.029826E-4 45.0 13 ATTCAAG 25 3.8879363E-5 45.0 41 GCGATTA 20 7.029826E-4 45.0 9 TGTAGCG 20 7.029826E-4 45.0 1 TTACGAG 20 7.029826E-4 45.0 12 CCGCGAA 20 7.029826E-4 45.0 15 CACAACG 20 7.029826E-4 45.0 12 TTACCGG 20 7.029826E-4 45.0 2 TCGTTCA 110 0.0 42.954544 16 CGTTCAT 110 0.0 42.954544 17 TACGGCT 1770 0.0 42.203392 7 ACGGCTG 1785 0.0 41.84874 8 TTTCGCG 125 0.0 41.4 1 TTACGGG 125 0.0 41.4 3 ATACGGC 3535 0.0 41.371994 6 TCGTTTG 120 0.0 41.250004 1 CTACGGC 60 3.6379788E-12 41.250004 6 >>END_MODULE