SRR2933792.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933792.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1082850
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC	39698	3.666066398854874	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	34014	3.141155284665466	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	26896	2.483815856305121	No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	13135	1.2130027242923767	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTATGCT	12655	1.1686752551138202	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTA	8397	0.7754536639423742	No Hit
GAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	8055	0.7438703421526527	No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	6721	0.6206769173939142	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTC	6681	0.6169829616290345	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT	6376	0.5888165489218267	No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	6251	0.5772729371565776	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	4996	0.46137507503347647	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGATTATGCTCGT	2949	0.272336888765757	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATG	2448	0.2260700928106386	No Hit
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	2228	0.20575333610380014	No Hit
TGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT	1958	0.18081913469086208	Illumina Single End Adapter 2 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTAT	1946	0.17971094796139817	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1908	0.17620168998476243	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGATTATGC	1572	0.14517246155977281	No Hit
TCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	1321	0.12199288913515262	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT	1265	0.116821351064321	No Hit
TTCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	1235	0.11405088424066123	No Hit
TGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	1199	0.11072632405226947	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	30	2.165385E-6	45.000004	2
CGCATGG	55	1.8189894E-12	45.000004	2
CATTCGG	55	1.8189894E-12	45.000004	3
GTCCGCG	30	2.165385E-6	45.000004	1
GGTACGA	25	3.890656E-5	45.0	8
ACCGGTG	80	0.0	45.0	17
GACCGGT	20	7.0331065E-4	45.0	14
TCGGCGT	25	3.890656E-5	45.0	4
CCGTACG	25	3.890656E-5	45.0	20
TCTACCG	25	3.890656E-5	45.0	1
TCGCTAG	20	7.0331065E-4	45.0	1
AGTTCGG	25	3.890656E-5	45.0	2
GCTAGCG	20	7.0331065E-4	45.0	1
CGTCCGC	20	7.0331065E-4	45.0	45
TCGGTTC	20	7.0331065E-4	45.0	35
CTAGCGG	40	6.8139343E-9	45.0	2
ACGGTAC	50	2.1827873E-11	45.0	15
TACGGCT	4395	0.0	43.873722	7
ACGGCTG	4435	0.0	43.478016	8
GCGACTG	955	0.0	43.350784	11

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content