##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933792.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1082850
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.547239229810224	30.0	16.0	33.0	16.0	34.0
2	27.720352772775545	31.0	25.0	33.0	16.0	34.0
3	28.68634436902618	31.0	28.0	34.0	16.0	34.0
4	32.35607424851087	35.0	28.0	37.0	26.0	37.0
5	33.77106247402688	35.0	33.0	37.0	28.0	37.0
6	32.767535669760356	35.0	32.0	37.0	25.0	37.0
7	33.552228840559636	35.0	32.0	35.0	29.0	37.0
8	33.35179757122408	35.0	33.0	37.0	28.0	37.0
9	34.70347047144111	37.0	32.0	39.0	29.0	39.0
10	33.60398670175925	35.0	32.0	37.0	27.0	39.0
11	34.28327838574133	35.0	32.0	39.0	27.0	39.0
12	34.46068892275015	37.0	33.0	39.0	27.0	39.0
13	34.91247633559588	37.0	34.0	39.0	30.0	39.0
14	34.692484646996355	37.0	33.0	39.0	27.0	40.0
15	35.18067691739392	37.0	33.0	39.0	27.0	40.0
16	34.67966292653645	36.0	32.0	39.0	27.0	40.0
17	34.42604608209817	36.0	32.0	38.0	27.0	40.0
18	34.61798125317449	37.0	32.0	39.0	27.0	40.0
19	34.44507641870989	37.0	32.0	39.0	27.0	40.0
20	35.00708685413492	37.0	33.0	39.0	27.0	40.0
21	34.48413076603408	37.0	32.0	39.0	26.0	40.0
22	35.548705730248884	37.0	34.0	39.0	30.0	40.0
23	36.19304428129473	38.0	34.0	39.0	30.0	40.0
24	36.33365101352911	38.0	35.0	40.0	31.0	40.0
25	34.207733296393776	37.0	32.0	39.0	24.0	40.0
26	35.013155099967676	37.0	33.0	39.0	27.0	40.0
27	35.585218636006836	37.0	34.0	39.0	30.0	40.0
28	35.78980375859999	38.0	34.0	40.0	30.0	40.0
29	36.29547121023226	38.0	35.0	40.0	30.0	41.0
30	33.67416354989149	37.0	31.0	39.0	23.0	40.0
31	34.963310707854276	37.0	33.0	39.0	27.0	40.0
32	35.530896246017456	37.0	34.0	39.0	30.0	40.0
33	36.14761231934248	38.0	35.0	40.0	30.0	40.0
34	36.50052269474073	38.0	35.0	40.0	31.0	41.0
35	36.58544581428637	38.0	35.0	40.0	31.0	41.0
36	36.49009096366071	38.0	35.0	40.0	30.0	41.0
37	36.65839312924228	39.0	35.0	40.0	31.0	41.0
38	36.31258900124671	38.0	35.0	40.0	30.0	41.0
39	36.31500761878377	38.0	35.0	40.0	30.0	41.0
40	35.90401071247172	38.0	35.0	40.0	30.0	41.0
41	35.575950500992754	38.0	34.0	40.0	30.0	41.0
42	36.48382139723877	38.0	35.0	40.0	31.0	41.0
43	36.4963346723923	38.0	35.0	40.0	31.0	41.0
44	36.952843884194486	39.0	36.0	40.0	32.0	41.0
45	37.18043311631343	39.0	36.0	40.0	33.0	41.0
46	36.91342937618322	39.0	36.0	40.0	32.0	41.0
47	36.68983331024611	39.0	35.0	40.0	31.0	41.0
48	36.83244401348294	39.0	35.0	40.0	32.0	41.0
49	37.05617029136076	39.0	36.0	40.0	32.0	41.0
50	36.83250773421988	39.0	35.0	40.0	31.0	41.0
51	36.18482430622893	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	13.0
11	7.0
12	24.0
13	20.0
14	28.0
15	62.0
16	115.0
17	276.0
18	586.0
19	1042.0
20	1686.0
21	2417.0
22	2996.0
23	3771.0
24	4912.0
25	7016.0
26	9073.0
27	12224.0
28	16099.0
29	22995.0
30	32704.0
31	45959.0
32	64472.0
33	86574.0
34	113976.0
35	150799.0
36	184869.0
37	192886.0
38	114111.0
39	11130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.56577549983839	3.451724615597728	49.21272567761001	11.76977420695387
2	17.29814840467285	19.17892598236136	44.328854411968415	19.194071200997367
3	16.1297501962414	19.17597081774946	45.986609410352315	18.707669575656833
4	15.393729510089116	3.16729002170199	60.20289052038602	21.236089947822876
5	27.244493697187977	4.1677055917255394	46.836403934062886	21.751396777023597
6	17.15888627233689	17.101352911298886	49.65803204506626	16.081728771297964
7	70.16484277600775	1.1106801496052083	23.80865309137923	4.915823983007804
8	69.7043911899155	12.61485893706423	10.271136353142172	7.4096135198781
9	66.111280417417	3.1746779332317496	11.407674193101537	19.306367456249713
10	36.95424112296255	17.942743685644363	25.472133721198688	19.630881470194396
11	28.778131781871913	24.37068846100568	24.97317264625756	21.878007110864846
12	20.00193932677656	18.913422911760634	33.632082005817985	27.452555755644823
13	21.68906127349125	22.646349909959827	39.14281756475966	16.52177125178926
14	21.549706792261166	26.657708823936833	28.628157177817798	23.164427205984207
15	15.567899524403195	25.211432793092303	38.650413261301196	20.57025442120331
16	16.19365563097382	24.427021286420096	27.910513921595786	31.4688091610103
17	16.996721614258668	26.938264764279445	33.010758646165215	23.05425497529667
18	16.977051299810686	24.23253451539918	33.36306967724062	25.42734450754952
19	18.350556402087083	27.659417278478088	28.735835988364038	25.254190331070784
20	18.772221452648104	25.9370180542088	37.606870757722675	17.68388973542042
21	21.600498684028256	29.96897077157501	29.085284203721663	19.34524634067507
22	17.661079558572286	23.277185205707163	31.87994643764141	27.181788798079143
23	18.041372304566654	30.10416955256961	28.43330101122039	23.42115713164335
24	21.638915823983005	23.856859214110912	26.848593988086993	27.65563097381909
25	17.13856951563005	31.847809022486956	24.75033476474119	26.2632866971418
26	19.231380154222652	24.531837281248556	30.77240615043635	25.464376414092442
27	20.840097889827767	28.55695618044974	26.58669252435702	24.01625340536547
28	16.269935817518586	24.84942512813409	35.73089532252851	23.149743731818813
29	23.07420233642702	21.790829754813686	30.108602299487465	25.026365609271828
30	17.98420833910514	31.882439857782703	30.31195456434409	19.821397238768064
31	20.365424574040723	23.928244909267214	31.497806713764604	24.20852380292746
32	22.15708546890151	30.1582860045251	28.035923719813454	19.64870480675994
33	16.76040079420049	26.200120053562358	26.597127949392807	30.442351202844346
34	24.737036523987623	22.977420695387174	31.185667451632266	21.099875328992933
35	21.135060257653414	26.03093687953087	25.98189961675209	26.85210324606363
36	22.661402779701714	24.71828969848086	28.266980652906682	24.353326868910745
37	16.42859121762017	27.153437687583693	36.749595973588214	19.668375121207923
38	18.53894814609595	27.805513228979084	30.423789075125825	23.23174954979914
39	19.22897908297548	22.969478690492682	36.837235074109984	20.96430715242185
40	22.34159855935725	26.59361869141617	29.512674885718244	21.552107863508336
41	19.607240153299163	24.081267026827355	29.83321789721568	26.478274922657803
42	23.797017130719862	20.459251050468673	31.63198965692386	24.111742161887612
43	22.357851964722723	21.59929814840467	26.45592649028028	29.586923396592326
44	19.657108556125042	22.46931707992797	31.947822874821075	25.92575148912592
45	20.017638638777303	19.66163365193702	30.581428637392065	29.739299071893615
46	25.68102691970264	24.134644687629866	29.24421665050561	20.94011174216189
47	15.976543380893013	22.67146880916101	39.59107909682781	21.76090871311816
48	19.822782472179895	24.4060580874544	29.939326776561852	25.83183266380385
49	20.117652491111418	18.92958396823198	37.51258253682413	23.440181003832482
50	22.22893290852842	18.539594588354806	31.257145495682686	27.974327007434084
51	19.74705637899986	18.763817703283003	28.493789536870295	32.99533638084684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	66.0
1	249.5
2	433.0
3	1912.5
4	3392.0
5	2485.0
6	1578.0
7	1588.0
8	1598.0
9	1714.0
10	1830.0
11	1823.5
12	1817.0
13	1892.0
14	1967.0
15	1910.0
16	1853.0
17	1821.0
18	1789.0
19	1846.0
20	1903.0
21	2141.5
22	2380.0
23	2577.5
24	2775.0
25	3479.0
26	5021.5
27	5860.0
28	7793.5
29	9727.0
30	10938.5
31	12150.0
32	15062.5
33	17975.0
34	20566.0
35	23157.0
36	26574.0
37	29991.0
38	35023.5
39	40056.0
40	46923.5
41	53791.0
42	63470.0
43	73149.0
44	154407.0
45	235665.0
46	195597.5
47	155530.0
48	143082.0
49	130634.0
50	105003.5
51	79373.0
52	67824.0
53	56275.0
54	47792.5
55	39310.0
56	34261.0
57	29212.0
58	25512.0
59	21812.0
60	18916.5
61	16021.0
62	13366.0
63	10711.0
64	8403.5
65	6096.0
66	4784.0
67	3472.0
68	2853.0
69	2234.0
70	1756.0
71	1278.0
72	1033.5
73	789.0
74	697.0
75	420.0
76	235.0
77	153.5
78	72.0
79	74.5
80	77.0
81	52.0
82	27.0
83	14.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1082850.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.171740606701576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.05083451138078	22.85829017789823
2	12.81154727737947	8.243395515566922
3	4.962440796822955	4.789510742745047
4	2.5405396235849165	3.269343270840847
5	1.4948040449751385	2.4045223996394216
6	0.9663948900773864	1.8654363436326933
7	0.7378296660793006	1.6616085240322747
8	0.5635676162221305	1.4504760930748422
9	0.4672050810019663	1.352772060851543
>10	4.203203475904926	25.855840268706622
>50	0.11588126698519896	2.5666094136680617
>100	0.07387397346120385	4.4863777235147815
>500	0.004924931563865889	1.024571013657357
>1k	0.0037661241370739156	2.431363961910258
>5k	0.001738211140187961	3.9593196789937393
>10k+	0.0014485092834899673	11.780562811267405
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC	39698	3.666066398854874	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	34014	3.141155284665466	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	26896	2.483815856305121	No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	13135	1.2130027242923767	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTATGCT	12655	1.1686752551138202	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTA	8397	0.7754536639423742	No Hit
GAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	8055	0.7438703421526527	No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	6721	0.6206769173939142	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTC	6681	0.6169829616290345	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT	6376	0.5888165489218267	No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	6251	0.5772729371565776	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	4996	0.46137507503347647	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGATTATGCTCGT	2949	0.272336888765757	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATG	2448	0.2260700928106386	No Hit
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	2228	0.20575333610380014	No Hit
TGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT	1958	0.18081913469086208	Illumina Single End Adapter 2 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTAT	1946	0.17971094796139817	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1908	0.17620168998476243	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGATTATGC	1572	0.14517246155977281	No Hit
TCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	1321	0.12199288913515262	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT	1265	0.116821351064321	No Hit
TTCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	1235	0.11405088424066123	No Hit
TGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	1199	0.11072632405226947	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.637299718335873	0.0
2	0.0	0.0	0.0	3.248464699635222	0.0
3	0.0	0.0	0.0	4.054116451955488	0.0
4	0.0	0.0	0.0	5.813178187191208	0.0
5	0.0	0.0	0.0	9.878653553123701	0.0
6	0.0	0.0	0.0	10.807314032414462	0.0
7	0.0	0.0	0.0	12.18026504132613	0.0
8	0.0	0.0	0.0	13.961859906727616	0.0
9	0.0	0.0	0.0	14.412614858937065	0.0
10	0.0	0.0	0.0	18.846008219051576	0.0
11	0.0	0.0	0.0	21.020178233365655	0.0
12	0.0	0.0	0.0	26.644687629865633	0.0
13	0.0	0.0	0.0	27.50510227640024	0.0
14	0.0	0.0	0.0	27.862123101075866	0.0
15	0.0	0.0	0.0	29.00410952578843	0.0
16	0.0	0.0	0.0	30.03379969524865	0.0
17	0.0	0.0	0.0	31.315232950085424	0.0
18	0.0	0.0	0.0	32.58586138430992	0.0
19	0.0	0.0	0.0	34.757999722953315	0.0
20	0.0	0.0	0.0	35.839497622015976	0.0
21	0.0	0.0	0.0	37.17957242462022	0.0
22	0.0	0.0	0.0	38.7247541210694	0.0
23	0.0	0.0	0.0	40.248510874082285	0.0
24	0.0	0.0	0.0	41.34552338735744	0.0
25	0.0	0.0	0.0	42.346862446322206	0.0
26	0.0	0.0	0.0	43.21660433116313	0.0
27	0.0	0.0	0.0	44.06011913007342	0.0
28	9.23488941219929E-5	0.0	0.0	44.98471625802281	0.0
29	1.846977882439858E-4	0.0	0.0	45.897492727524586	0.0
30	1.846977882439858E-4	0.0	0.0	46.83003186036847	0.0
31	1.846977882439858E-4	0.0	0.0	47.764510319988915	0.0
32	1.846977882439858E-4	0.0	0.0	48.649028027889365	0.0
33	1.846977882439858E-4	0.0	0.0	49.52126333287159	0.0
34	1.846977882439858E-4	0.0	0.0	50.39322159117145	0.0
35	1.846977882439858E-4	0.0	0.0	51.196656970032784	0.0
36	1.846977882439858E-4	0.0	0.0	52.01782333656554	0.0
37	1.846977882439858E-4	0.0	0.0	52.84305305443967	0.0
38	1.846977882439858E-4	0.0	0.0	53.67059149466685	0.0
39	2.770466823659787E-4	0.0	0.0	54.53146788567207	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	30	2.165385E-6	45.000004	2
CGCATGG	55	1.8189894E-12	45.000004	2
CATTCGG	55	1.8189894E-12	45.000004	3
GTCCGCG	30	2.165385E-6	45.000004	1
GGTACGA	25	3.890656E-5	45.0	8
ACCGGTG	80	0.0	45.0	17
GACCGGT	20	7.0331065E-4	45.0	14
TCGGCGT	25	3.890656E-5	45.0	4
CCGTACG	25	3.890656E-5	45.0	20
TCTACCG	25	3.890656E-5	45.0	1
TCGCTAG	20	7.0331065E-4	45.0	1
AGTTCGG	25	3.890656E-5	45.0	2
GCTAGCG	20	7.0331065E-4	45.0	1
CGTCCGC	20	7.0331065E-4	45.0	45
TCGGTTC	20	7.0331065E-4	45.0	35
CTAGCGG	40	6.8139343E-9	45.0	2
ACGGTAC	50	2.1827873E-11	45.0	15
TACGGCT	4395	0.0	43.873722	7
ACGGCTG	4435	0.0	43.478016	8
GCGACTG	955	0.0	43.350784	11
>>END_MODULE