FastQCFastQC Report
Sat 14 Jan 2017
SRR2933791.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933791.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218854
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGC384273.1527155836548104No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCG315602.589317506444578No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC246412.0216531266255022No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC111820.9174191494633483Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTCTTCT110440.9060970386937237No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTA73210.600646180756678No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT71150.5837450588831805TruSeq Adapter, Index 23 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTC55180.45272034222310464No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT50020.41038549325842144No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC40450.3318691163995031Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT35170.2885497360635482TruSeq Adapter, Index 23 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30580.2508914111124056No Hit
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC27790.22800105673033852Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGT25720.21101789057590162No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATG23000.1887018461604097No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG18060.14817197137639126No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTAT16570.1359473735164343No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC15250.12511752843244556No Hit
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG13450.11034955786337002TruSeq Adapter, Index 23 (95% over 24bp)
TGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTT12990.10657552094016183Illumina PCR Primer Index 7 (96% over 25bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAAGTCTTC12860.10550894528795081No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGT207.033454E-445.00000421
ATCGTAC207.033454E-445.00000442
TCGTACT207.033454E-445.00000443
GTACGAG207.033454E-445.00000434
CGAATAG253.8909446E-545.026
GCGATTA453.8562575E-1045.09
TACGGCT43300.044.0646677
ACGGCTG44200.043.1674238
TGATACC36950.042.1380234
AATGATC8400.042.053572
GATACCT37150.041.9111755
CGGCTGT47200.041.329459
ATGATAC133000.041.2951133
AATGATA132450.041.0079272
GAATGAT138200.040.9786531
CACCGGT1650.040.9090916
TGATACG96650.040.9027444
CGTTTTT19000.040.8552631
GATACGG97000.040.847945
ATACGGC96650.040.83296
Produced by FastQC (version 0.11.5)