SRR2933790.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933790.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	726855
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGC	22647	3.115752110118249	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG	18955	2.6078103610761434	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC	16018	2.20374077360684	No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	7934	1.0915519601571153	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGGGTGAT	7799	1.0729787921937664	No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	5130	0.7057803826072601	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT	4820	0.6631308858025329	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTA	4796	0.6598289892757152	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTC	4198	0.577556734149177	No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	3693	0.5080793280640569	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCT	3449	0.474510046708078	No Hit
TCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	2154	0.2963452132818788	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGT	1701	0.23402191633819674	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATG	1409	0.19384884192858273	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1404	0.19316094681882906	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG	1304	0.17940304462375575	No Hit
TGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTT	1204	0.16564514242868247	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTAT	1008	0.1386796541263388	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCGGGTGA	868	0.11941859105323621	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTAG	30	2.1643955E-6	45.000004	33
TCGAATG	30	2.1643955E-6	45.000004	44
GTTACCG	20	7.031573E-4	45.0	38
CGAATGC	20	7.031573E-4	45.0	45
CCGGTAT	20	7.031573E-4	45.0	42
TCGACAA	25	3.8893857E-5	45.0	19
CGTCATA	20	7.031573E-4	45.0	38
TACCGGT	20	7.031573E-4	45.0	40
TACGGCC	185	0.0	42.567566	7
TACGGCT	2790	0.0	42.258064	7
ACGGCTG	2805	0.0	42.1123	8
GGCGACT	645	0.0	41.860466	10
GATACCT	2225	0.0	41.561794	5
TGATACC	2215	0.0	41.546276	4
ATGATAC	8040	0.0	41.02612	3
GAATGAT	8360	0.0	40.882175	1
AATGATA	8025	0.0	40.710278	2
CGGCTGT	2965	0.0	40.370995	9
ATACGGC	5940	0.0	40.265152	6
TGATACG	6045	0.0	39.677418	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content