SRR2933789.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933789.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	258397
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGC	8583	3.3216329910950977	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCG	6587	2.549178202533311	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC	5377	2.0809065120725085	No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	3903	1.5104664527838945	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAACGGAAT	2641	1.0220706896751899	No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	2563	0.9918845807033363	No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	1900	0.7353026544425825	No Hit
GAACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT	1761	0.6815094602491515	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAACGGAATCGTA	1696	0.6563543694392737	No Hit
TCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	1409	0.5452849684787362	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTC	1337	0.5174208678893331	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCT	1303	0.5042628203887816	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAACGGAATCGT	629	0.2434238787602023	No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG	547	0.21168976420004876	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	537	0.20781975022929833	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATG	513	0.1985317166994973	No Hit
TGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTT	465	0.1799556496398952	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTAT	382	0.1478345336826666	No Hit
TTCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT	347	0.13428948478504008	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACGGAATCGTATGCCGTCTTC	333	0.12887146522598947	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGAACGGAA	326	0.12616245544646415	No Hit
TCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG	309	0.11958343169618843	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT	303	0.11726142331373815	No Hit
TGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG	292	0.11300440794591267	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCC	270	0.10449037721026173	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTGTC	20	7.023124E-4	45.000004	12
CGGGTAT	20	7.023124E-4	45.000004	6
AACCGCG	20	7.023124E-4	45.000004	12
ACGCCCG	20	7.023124E-4	45.000004	29
CTCGTGC	20	7.023124E-4	45.000004	32
TCCGCAG	20	7.023124E-4	45.000004	1
GATCGGA	20	7.023124E-4	45.000004	45
GGTCGGC	20	7.023124E-4	45.000004	2
GGACTAC	20	7.023124E-4	45.000004	8
CCAATAC	20	7.023124E-4	45.000004	16
CACGCCC	20	7.023124E-4	45.000004	28
TACGGTT	20	7.023124E-4	45.000004	33
CTAAGAT	20	7.023124E-4	45.000004	41
ACTTATG	20	7.023124E-4	45.000004	20
CCTCGTC	20	7.023124E-4	45.000004	36
TGACGGA	20	7.023124E-4	45.000004	25
TGCATTG	20	7.023124E-4	45.000004	1
ATTCCAC	20	7.023124E-4	45.000004	10
TCGGAAA	20	7.023124E-4	45.000004	25
CTTATTG	20	7.023124E-4	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content