##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933789.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	258397
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.61083913512928	30.0	16.0	33.0	16.0	34.0
2	27.86024992550223	31.0	25.0	33.0	16.0	34.0
3	28.833666799537145	31.0	28.0	34.0	16.0	34.0
4	32.38472969887421	35.0	30.0	35.0	26.0	37.0
5	33.72770968703197	35.0	33.0	37.0	28.0	37.0
6	32.772361908226486	35.0	32.0	37.0	25.0	37.0
7	33.4786588079583	35.0	32.0	35.0	29.0	37.0
8	33.364826217022646	35.0	33.0	37.0	28.0	37.0
9	34.63030143538818	37.0	32.0	39.0	28.0	39.0
10	33.84226984059413	35.0	32.0	39.0	27.0	39.0
11	34.2438611903389	35.0	32.0	39.0	27.0	39.0
12	34.362504208640196	37.0	32.0	39.0	27.0	39.0
13	34.723711962600184	37.0	34.0	39.0	28.0	39.0
14	34.40877409567449	37.0	32.0	39.0	25.0	40.0
15	34.89821863256926	37.0	32.0	39.0	27.0	40.0
16	34.35994225939156	36.0	32.0	39.0	27.0	40.0
17	34.41906833283668	36.0	32.0	38.0	27.0	40.0
18	34.25097814603111	36.0	32.0	39.0	27.0	40.0
19	33.956973184673195	36.0	32.0	39.0	25.0	40.0
20	34.99309976509015	37.0	33.0	39.0	27.0	40.0
21	34.5299945432803	37.0	32.0	39.0	26.0	40.0
22	35.55287019586141	37.0	34.0	39.0	29.0	40.0
23	36.281551256400036	38.0	34.0	40.0	31.0	40.0
24	36.38913377477293	38.0	35.0	40.0	31.0	40.0
25	34.17719245966478	37.0	32.0	39.0	24.0	40.0
26	35.07519437145168	37.0	33.0	39.0	27.0	40.0
27	35.45500528256907	37.0	34.0	39.0	30.0	40.0
28	35.55490195319605	38.0	34.0	39.0	29.0	40.0
29	36.16006764784421	38.0	35.0	40.0	30.0	41.0
30	33.61970533713627	37.0	31.0	39.0	23.0	40.0
31	34.648560161302186	37.0	32.0	39.0	26.0	40.0
32	35.2007801948165	37.0	34.0	39.0	29.0	40.0
33	35.93503020545904	38.0	35.0	39.0	30.0	40.0
34	36.18036587112079	38.0	35.0	40.0	30.0	41.0
35	36.24109413034981	38.0	35.0	40.0	30.0	41.0
36	36.258180242030676	38.0	35.0	40.0	30.0	41.0
37	36.343448259848216	38.0	35.0	40.0	30.0	41.0
38	35.85480868585935	38.0	34.0	40.0	30.0	41.0
39	35.914476561260386	38.0	34.0	40.0	30.0	41.0
40	35.51281942127811	38.0	34.0	40.0	28.0	41.0
41	35.03209015584546	37.0	33.0	40.0	27.0	41.0
42	35.81679740863864	38.0	34.0	40.0	30.0	41.0
43	35.944759420581505	38.0	35.0	40.0	30.0	41.0
44	36.37537200509294	38.0	35.0	40.0	31.0	41.0
45	36.61068820458442	38.0	35.0	40.0	31.0	41.0
46	36.353699926856734	38.0	35.0	40.0	31.0	41.0
47	36.004779467253876	38.0	34.0	40.0	30.0	41.0
48	36.11515613571365	38.0	35.0	40.0	31.0	41.0
49	36.299999612998604	38.0	35.0	40.0	31.0	41.0
50	36.00510841844139	38.0	35.0	40.0	30.0	41.0
51	35.29713580266025	37.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	7.0
12	14.0
13	9.0
14	8.0
15	12.0
16	37.0
17	92.0
18	149.0
19	262.0
20	407.0
21	532.0
22	709.0
23	863.0
24	1291.0
25	1819.0
26	2443.0
27	3383.0
28	4686.0
29	6314.0
30	8901.0
31	12347.0
32	17189.0
33	22440.0
34	28113.0
35	36130.0
36	42650.0
37	42419.0
38	22828.0
39	2337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.51355859394653	3.9559282809010945	45.455636094846305	16.074877030306077
2	17.90965065383886	17.547804347573695	45.02529054129885	19.51725445728859
3	18.21035073936617	17.54664334338247	45.80006733824309	18.44293857900827
4	18.272270962898176	3.3804572034505043	56.163190749118606	22.184081084532714
5	26.505725685669724	4.6536917998273974	44.28650487428259	24.55407764022028
6	18.79433584755241	16.16233934604504	47.32949685948366	17.71382794691889
7	69.03485721583455	1.6188268439649065	23.582704133561922	5.763611806638623
8	66.72407187389946	12.008653351238598	11.775291508802347	9.491983266059592
9	61.727883837660656	4.739993111375132	14.102330909414585	19.429792141549633
10	34.44544634806132	26.663622255676344	20.85473128557994	18.036200110682397
11	29.667527099772826	22.80444432404401	27.1373119656962	20.390716610486965
12	23.851670104529077	17.51065221345449	33.381192506104945	25.256485175911486
13	21.96194228261164	22.4774281435156	36.976048483535024	18.584581090337736
14	19.036985723518463	27.09087179804719	28.473627789796318	25.398514688638024
15	14.17353916647639	23.31722117516844	40.62160164398193	21.88763801437323
16	14.966505029083155	23.464668707454035	29.49260246829491	32.076223795167905
17	15.752117865145493	24.892703862660944	33.51896500346366	25.836213268729903
18	15.506758979399915	22.626036679992414	33.88622932928788	27.980975011319792
19	17.17589600498458	25.348591508415346	29.724803306539936	27.75070918006014
20	19.5884627143504	24.410113120508363	37.150973115012945	18.850451050128292
21	22.41395991439529	28.181054733607585	28.356753367879655	21.048231984117464
22	17.087272685054394	23.458089683703758	31.44076750117068	28.013870130071172
23	17.899975618911984	27.753418189839664	28.788646927015403	25.55795926423294
24	22.474332132338997	21.533144734652492	27.373769819309047	28.618753313699465
25	16.660023142683546	30.451204928849794	25.08659156259554	27.80218036587112
26	20.554805202846783	24.21467741498547	28.203887816035017	27.026629566132733
27	20.100078561283606	29.915207993900854	25.624136503132778	24.36057694168276
28	16.702980297758874	26.93452323362887	32.306489626427556	24.0560068421847
29	23.10978842633622	21.452261442663808	26.56996791758418	28.86798221341579
30	21.678270258555635	29.380759064540225	26.868345994729044	22.072624682175103
31	24.811433569275184	28.302186170892078	21.489026575385935	25.3973536844468
32	22.617522649256763	33.306501236469465	20.451475829827746	23.624500284446025
33	23.190284716927827	27.580041563950047	21.319906964864145	27.90976675425798
34	28.078112361985625	25.58698436901357	23.936423410488512	22.39847985851229
35	19.74713328715117	29.344767934612243	22.578435508152182	28.329663270084403
36	26.33351006397133	30.66792571121182	22.26728638490385	20.731277839913
37	18.505632805334425	30.218617089207694	29.038262828128808	22.23748727732907
38	22.144993943428133	31.298737988444138	21.96039427702334	24.595873791104385
39	25.83698727152405	23.590831162900496	26.041711010576744	24.530470554998704
40	27.8397195013874	25.328854437164516	26.016168918369797	20.815257143078288
41	23.918234344825983	30.133863783248255	22.677507865803396	23.27039400612236
42	20.000232200838244	28.218593869123872	27.962785945657266	23.818387984380625
43	24.136890134173385	27.551403460566494	23.300967116491293	25.010739288768836
44	21.888412017167383	22.364423735569687	29.412493179100373	26.334671068162557
45	21.278497815377115	21.65892018870188	25.18721192583505	31.87537007008595
46	27.09125879944427	24.60438782184004	26.30487196058778	21.99948141812792
47	18.14069048789266	22.815667364559186	36.601431131166386	22.44221101638177
48	21.22122160861001	25.472818956876438	26.29442292286675	27.011536511646806
49	19.92321892282031	21.23592766169886	35.26356730147796	23.57728611400287
50	24.9337260107509	20.958060658598978	27.538632414463017	26.569580916187107
51	20.753336919546282	19.466944275668833	25.596659403940446	34.183059400844435
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	37.0
1	107.0
2	177.0
3	571.0
4	965.0
5	682.0
6	399.0
7	411.5
8	424.0
9	471.0
10	518.0
11	546.5
12	575.0
13	570.5
14	566.0
15	533.0
16	500.0
17	519.0
18	538.0
19	532.5
20	527.0
21	630.0
22	733.0
23	802.0
24	871.0
25	969.0
26	1341.0
27	1615.0
28	1968.0
29	2321.0
30	2381.0
31	2441.0
32	2961.5
33	3482.0
34	3917.0
35	4352.0
36	5177.0
37	6002.0
38	6192.5
39	6383.0
40	7714.5
41	9046.0
42	10677.0
43	12308.0
44	14382.5
45	16457.0
46	33739.5
47	51022.0
48	44062.5
49	37103.0
50	34838.5
51	32574.0
52	25487.0
53	18400.0
54	15273.0
55	12146.0
56	10422.0
57	8698.0
58	8174.5
59	7651.0
60	6852.5
61	6054.0
62	5193.5
63	4333.0
64	3713.0
65	3093.0
66	2538.0
67	1983.0
68	1569.5
69	1156.0
70	995.5
71	835.0
72	663.0
73	491.0
74	378.0
75	201.0
76	137.0
77	118.0
78	99.0
79	59.0
80	19.0
81	26.0
82	33.0
83	17.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	258397.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.800315019137216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.73427678356748	24.24641152954562
2	12.073644076529385	8.161859464312666
3	4.750455123140865	4.8170063893930655
4	2.3872496822725244	3.227591651605862
5	1.3705217600384707	2.316203361494135
6	0.9846689336951419	1.9969272089072243
7	0.7041527839796655	1.666041014408062
8	0.5484376967906662	1.4829893535915664
9	0.4682902254433873	1.4245521426332348
>10	4.801978497578401	29.086638002763188
>50	0.103046748875073	2.4094706981892204
>100	0.0549582660667056	3.1865695035159076
>500	0.0045798555055587995	0.8614651098890467
>1k	0.010304674887507298	7.1645568640502795
>5k	0.0034348916291691	7.951717705700917
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGC	8583	3.3216329910950977	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCG	6587	2.549178202533311	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC	5377	2.0809065120725085	No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	3903	1.5104664527838945	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAACGGAAT	2641	1.0220706896751899	No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	2563	0.9918845807033363	No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	1900	0.7353026544425825	No Hit
GAACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT	1761	0.6815094602491515	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAACGGAATCGTA	1696	0.6563543694392737	No Hit
TCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	1409	0.5452849684787362	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTC	1337	0.5174208678893331	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCT	1303	0.5042628203887816	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAACGGAATCGT	629	0.2434238787602023	No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG	547	0.21168976420004876	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	537	0.20781975022929833	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATG	513	0.1985317166994973	No Hit
TGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTT	465	0.1799556496398952	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTAT	382	0.1478345336826666	No Hit
TTCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT	347	0.13428948478504008	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACGGAATCGTATGCCGTCTTC	333	0.12887146522598947	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGAACGGAA	326	0.12616245544646415	No Hit
TCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG	309	0.11958343169618843	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT	303	0.11726142331373815	No Hit
TGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG	292	0.11300440794591267	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCC	270	0.10449037721026173	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8649481224627221	0.0
2	0.0	0.0	0.0	4.373115786947991	0.0
3	0.0	0.0	0.0	5.33984527684145	0.0
4	0.0	0.0	0.0	7.378568636632778	0.0
5	0.0	0.0	0.0	11.62590896953138	0.0
6	0.0	0.0	0.0	12.70099885060585	0.0
7	0.0	0.0	0.0	14.147997074269439	0.0
8	0.0	0.0	0.0	16.097710112733505	0.0
9	0.0	0.0	0.0	16.72620038158338	0.0
10	0.0	0.0	0.0	20.744435887413555	0.0
11	0.0	0.0	0.0	23.050964213980812	0.0
12	0.0	0.0	0.0	28.889267290254917	0.0
13	0.0	0.0	0.0	29.878829862575802	0.0
14	0.0	0.0	0.0	30.269314272224523	0.0
15	0.0	0.0	0.0	31.390457319550922	0.0
16	0.0	0.0	0.0	32.489541287244045	0.0
17	0.0	0.0	0.0	33.8037980317109	0.0
18	0.0	0.0	0.0	35.11495876500114	0.0
19	0.0	0.0	0.0	37.11807799626157	0.0
20	0.0	0.0	0.0	38.28604821263405	0.0
21	0.0	0.0	0.0	39.479173519816406	0.0
22	0.0	0.0	0.0	40.852641478035736	0.0
23	0.0	0.0	0.0	42.03918776146782	0.0
24	0.0	0.0	0.0	42.96644310885962	0.0
25	0.0	0.0	0.0	43.74895993374536	0.0
26	0.0	0.0	0.0	44.49084161193822	0.0
27	0.0	0.0	0.0	45.14951798975994	0.0
28	0.0	0.0	0.0	45.83296245699447	0.0
29	0.0	0.0	0.0	46.58955018827618	0.0
30	0.0	0.0	0.0	47.49900347140253	0.0
31	0.0	0.0	0.0	48.23275812025681	0.0
32	0.0	0.0	0.0	48.97850981242042	0.0
33	0.0	0.0	0.0	49.61125709663812	0.0
34	0.0	0.0	0.0	50.341528732918725	0.0
35	0.0	0.0	0.0	51.02845621272693	0.0
36	0.0	0.0	0.0	51.744021795918684	0.0
37	0.0	0.0	0.0	52.431723278521034	0.0
38	0.0	0.0	0.0	53.04860350545866	0.0
39	0.0	0.0	0.0	53.723146940560454	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTGTC	20	7.023124E-4	45.000004	12
CGGGTAT	20	7.023124E-4	45.000004	6
AACCGCG	20	7.023124E-4	45.000004	12
ACGCCCG	20	7.023124E-4	45.000004	29
CTCGTGC	20	7.023124E-4	45.000004	32
TCCGCAG	20	7.023124E-4	45.000004	1
GATCGGA	20	7.023124E-4	45.000004	45
GGTCGGC	20	7.023124E-4	45.000004	2
GGACTAC	20	7.023124E-4	45.000004	8
CCAATAC	20	7.023124E-4	45.000004	16
CACGCCC	20	7.023124E-4	45.000004	28
TACGGTT	20	7.023124E-4	45.000004	33
CTAAGAT	20	7.023124E-4	45.000004	41
ACTTATG	20	7.023124E-4	45.000004	20
CCTCGTC	20	7.023124E-4	45.000004	36
TGACGGA	20	7.023124E-4	45.000004	25
TGCATTG	20	7.023124E-4	45.000004	1
ATTCCAC	20	7.023124E-4	45.000004	10
TCGGAAA	20	7.023124E-4	45.000004	25
CTTATTG	20	7.023124E-4	45.000004	1
>>END_MODULE