FastQCFastQC Report
Sat 14 Jan 2017
SRR2933788.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933788.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275119
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC110924.031709914618765No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG94103.420338108236799No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC73432.6690268574689497No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC44511.617845368731349Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAAGAT38211.388853550645357No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC28961.0526354050429088Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTA23060.8381827500099956No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT21390.7774817442633914Illumina Single End Adapter 1 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT20500.745132106470291No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC18510.6727997702812237Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTC18500.6724362912048968No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCT17680.6426310069460851No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGT7880.28642151214565337No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG7490.27224582816890147No Hit
TGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT6920.25152752081826407Illumina Single End Adapter 1 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATG6260.22753790178068398No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTAT5070.18428389169777443No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTT5030.18282997539246654No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.18246649631613956No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCAGCAAGA4920.17883170555286987No Hit
GCTGTCTCTTATACACATCTGACGCCAGCCAGATCGTATGCCGTCTTCTGC4490.16320210527081008Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG4230.15375164928630883No Hit
TGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG3960.14393771422548063No Hit
TTCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT3540.12867159301974782No Hit
GGGCAAACTGAAGTAGTGATGATAAAAAAGTTAAACAAACAAACAAACAAA3510.1275811557907669No Hit
CCTGTCTCTTATACACATCTGACGCCAGCCAGATCGTATGCCGTCTTCTGC3250.11813069980626564Illumina Single End Adapter 1 (95% over 21bp)
TTTTTTGGGCATGTTGCTTTTGGAATCAGCCTGACTCTTTGCCCAGTATAT3130.11376895089034199No Hit
TTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG3090.1123150345850341No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC2860.10395501582951379No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGCG302.1594624E-645.00000424
GGAGTTC351.207427E-745.0000048
TTGCGAC302.1594624E-645.00000426
CAGCTAT351.207427E-745.00000416
GGAATGC253.883046E-545.08
TCGTTTG551.8189894E-1245.01
TGATAAG207.0239237E-445.026
CCGGGTG207.0239237E-445.05
ACCGGTG207.0239237E-445.017
ATTAGCG207.0239237E-445.015
GGTAAGT207.0239237E-445.09
GTCGACT207.0239237E-445.012
TTAGCGT207.0239237E-445.016
TTTGTCG502.1827873E-1145.01
TTCACGA207.0239237E-445.032
CCCAGTT207.0239237E-445.013
CACGATA207.0239237E-445.034
TATGCGG207.0239237E-445.02
ACGGGAT253.883046E-545.05
GACTTTA207.0239237E-445.041
Produced by FastQC (version 0.11.5)