##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933788.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	275119
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.256808871797293	30.0	16.0	33.0	16.0	34.0
2	27.343531344618146	31.0	25.0	33.0	16.0	34.0
3	28.34609023731549	31.0	26.0	34.0	16.0	34.0
4	32.113743507355	35.0	28.0	35.0	25.0	37.0
5	33.65634870728667	35.0	33.0	37.0	28.0	37.0
6	32.47834209923706	35.0	32.0	37.0	23.0	37.0
7	33.440489388228364	35.0	32.0	35.0	29.0	37.0
8	33.053580450641356	35.0	33.0	37.0	27.0	37.0
9	34.616271504330854	37.0	32.0	39.0	28.0	39.0
10	33.83159287435619	35.0	32.0	39.0	27.0	39.0
11	34.26287897237196	35.0	32.0	39.0	27.0	39.0
12	34.430039364783966	37.0	33.0	39.0	27.0	39.0
13	34.72007385894831	37.0	34.0	39.0	28.0	39.0
14	34.485698915741914	37.0	33.0	39.0	26.0	40.0
15	34.949225607827884	37.0	32.0	39.0	27.0	40.0
16	34.45348739999782	36.0	32.0	39.0	27.0	40.0
17	34.36061849599628	36.0	32.0	38.0	27.0	40.0
18	34.36641962205446	36.0	32.0	39.0	27.0	40.0
19	34.0108752939637	36.0	32.0	39.0	25.0	40.0
20	34.833301226014925	37.0	32.0	39.0	27.0	40.0
21	34.42703339282274	37.0	32.0	39.0	26.0	40.0
22	35.48869761812161	37.0	34.0	39.0	29.0	40.0
23	36.13364035199314	38.0	34.0	39.0	30.0	40.0
24	36.29031073826235	38.0	35.0	40.0	31.0	40.0
25	34.270682868140696	37.0	32.0	39.0	25.0	40.0
26	35.13694074200619	37.0	33.0	39.0	27.0	40.0
27	35.52803332376172	37.0	34.0	39.0	30.0	40.0
28	35.704436989084726	38.0	34.0	40.0	30.0	40.0
29	36.138907163809115	38.0	35.0	40.0	30.0	41.0
30	33.67315234498526	37.0	31.0	39.0	23.0	40.0
31	34.588847008021986	37.0	33.0	39.0	25.0	40.0
32	35.161628240870314	37.0	34.0	39.0	29.0	40.0
33	35.758820728484764	38.0	34.0	39.0	30.0	40.0
34	36.09139681374241	38.0	35.0	40.0	30.0	40.0
35	36.301527702557806	38.0	35.0	40.0	30.0	41.0
36	36.30257815708839	38.0	35.0	40.0	30.0	41.0
37	36.486178708122665	38.0	35.0	40.0	31.0	41.0
38	35.97566144104915	38.0	35.0	40.0	30.0	41.0
39	35.97733344480025	38.0	35.0	40.0	30.0	41.0
40	35.59219828510572	38.0	34.0	40.0	30.0	41.0
41	35.04978209429374	37.0	33.0	40.0	27.0	40.0
42	36.11800348212955	38.0	35.0	40.0	30.0	41.0
43	36.236043312166736	38.0	35.0	40.0	31.0	41.0
44	36.626441648886484	38.0	35.0	40.0	31.0	41.0
45	36.895935940447586	39.0	35.0	40.0	32.0	41.0
46	36.65645775100956	39.0	35.0	40.0	32.0	41.0
47	36.40359262719041	38.0	35.0	40.0	31.0	41.0
48	36.55916530664912	38.0	35.0	40.0	31.0	41.0
49	36.84830200749494	39.0	35.0	40.0	32.0	41.0
50	36.65301560415675	39.0	35.0	40.0	31.0	41.0
51	36.06425583111308	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	5.0
11	3.0
12	10.0
13	10.0
14	11.0
15	23.0
16	34.0
17	75.0
18	157.0
19	236.0
20	435.0
21	573.0
22	782.0
23	981.0
24	1311.0
25	1747.0
26	2437.0
27	3367.0
28	4661.0
29	6409.0
30	9227.0
31	13195.0
32	18044.0
33	24027.0
34	30291.0
35	39040.0
36	45834.0
37	44802.0
38	24985.0
39	2403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.06468837121391	3.5137522308528313	43.752339896553856	15.669219501379402
2	17.43609129140481	21.428545465780264	41.28722480090434	19.848138441910592
3	17.29833272147689	20.98691838804299	44.56108084138137	17.15366804909875
4	17.385567699795363	3.6522377589334067	57.98509008828907	20.977104452982164
5	28.871143032651325	4.484604843722171	43.16713858366743	23.47711353995907
6	17.237268236653957	18.98669303101567	45.212435346159296	18.563603386171078
7	65.10019300738954	1.8631937452520546	26.884002922371774	6.152610324986642
8	62.300677161519204	14.745619168432569	12.596730869187514	10.356972800860719
9	58.350386559997666	4.263609565315372	14.711088656181506	22.674915218505447
10	35.613679898516644	24.038325233807917	22.180583674700767	18.167411192974676
11	30.177486832970462	23.345534114328707	27.09772861925203	19.3792504334488
12	23.039848211137727	17.772672916083586	32.86432416517943	26.323154707599254
13	22.970060228482946	22.306710914186226	38.36485302723548	16.35837583009534
14	19.475935867751772	27.430675453167535	27.17842097419662	25.914967704884067
15	13.172118247013112	24.45923400419455	40.56208404363203	21.80656370516031
16	14.129522134058353	24.82125916421621	27.868304261065212	33.180914440660224
17	15.099647788775039	26.327152977438857	33.49677775798836	25.076421475797744
18	14.936082204427903	22.922080990407785	34.59375761034316	27.548079194821153
19	16.730214925177833	25.23308095769467	30.049542198103367	27.987161919024135
20	18.969609514428303	24.221518688276706	39.14596956226215	17.66290223503284
21	21.954863168301717	29.724228424790727	28.00170108207721	20.319207324830344
22	16.549565824243327	23.459666544295377	32.30674726209386	27.684020369367435
23	18.034014371962677	29.431991247423845	28.28957651052817	24.244417870085307
24	21.930510070187808	22.240921201371044	26.09016461967367	29.73840410876748
25	14.679465976541062	31.91528029688971	24.153911580079892	29.25134214648934
26	15.979630632562635	23.091825719052483	29.308408361472672	31.62013528691221
27	19.749635612225983	29.84817478981822	24.572275996932234	25.829913601023556
28	16.98973898567529	24.371635546799748	32.22023924192804	26.418386225596922
29	19.26511800348213	21.430362861161896	25.333401182760912	33.97111795259506
30	18.464010119257484	33.50768213027817	25.902609416288954	22.125698334175393
31	24.178991636346453	27.500099956745988	20.87533031161061	27.445578095296945
32	23.94418415303923	31.062194904750307	20.303577724548287	24.690043217662176
33	18.077995340198243	31.689559790490662	20.605628836976	29.626816032335096
34	22.81267378843337	27.41104758304588	23.652310454748672	26.123968173772077
35	19.300738952962174	28.69231132709846	21.717147852383878	30.289801867555493
36	22.831938179478698	28.84424558100313	21.56448664032655	26.759329599191624
37	17.93333066782011	29.75803197888914	28.39207761005238	23.91655974323838
38	24.517390656406864	29.04743038466991	21.756767071703518	24.678411887219713
39	19.569349990367805	29.195002889658657	25.621276611211876	25.614370508761663
40	23.603604258520857	28.135097903089207	25.524954655985226	22.736343182404706
41	19.86740283295592	33.96566576645015	21.36530010649937	24.801631294094555
42	24.787092131041476	21.381656664934084	27.74363093788506	26.08762026613938
43	22.97551241462785	26.913808206630584	22.239467285065736	27.87121209367583
44	20.405351865919837	22.965335000490697	28.838793394858225	27.79051973873124
45	20.218887099764103	20.356282190615698	25.577659122052637	33.84717158756756
46	26.077806331078552	25.325041164005395	25.359571676256454	23.2375808286596
47	14.987332754190005	23.71373841864793	38.63019275295418	22.66873607420789
48	20.293036831334803	26.17049349554193	25.518048553535017	28.01842111958825
49	20.437701503712937	19.326909446457716	35.45702041661972	24.77836863320963
50	23.195053776729342	21.12504043704724	27.933003536651412	27.746902249572003
51	19.45376364409583	19.87358197725348	25.391921314049558	35.28073306460114
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	50.0
1	137.0
2	224.0
3	593.0
4	962.0
5	682.5
6	403.0
7	458.0
8	513.0
9	585.5
10	658.0
11	614.5
12	571.0
13	607.0
14	643.0
15	558.5
16	474.0
17	530.5
18	587.0
19	594.5
20	602.0
21	678.5
22	755.0
23	819.5
24	884.0
25	898.0
26	1168.0
27	1424.0
28	2107.0
29	2790.0
30	2990.5
31	3191.0
32	3554.0
33	3917.0
34	4284.0
35	4651.0
36	5075.5
37	5500.0
38	6306.5
39	7113.0
40	7742.5
41	8372.0
42	10110.5
43	11849.0
44	13907.5
45	15966.0
46	38377.0
47	60788.0
48	50620.5
49	40453.0
50	38509.5
51	36566.0
52	28062.5
53	19559.0
54	16012.0
55	12465.0
56	10528.0
57	8591.0
58	7857.5
59	7124.0
60	6344.5
61	5565.0
62	4602.0
63	3639.0
64	3123.5
65	2608.0
66	2274.5
67	1941.0
68	1574.5
69	1208.0
70	948.0
71	688.0
72	569.0
73	450.0
74	342.0
75	198.5
76	163.0
77	109.0
78	55.0
79	32.5
80	10.0
81	5.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	275119.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.739788964048287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.84816308190751	21.778575816283137
2	12.503103900864362	7.686855506162788
3	4.969788698253538	4.58310767340678
4	2.600182095517376	3.1971619553720387
5	1.50760899126178	2.317179111584442
6	0.9695995081056153	1.7883170555286982
7	0.6822669709474879	1.468091989284637
8	0.5391919215806837	1.325971670440791
9	0.411488571732627	1.138416467056074
>10	4.7581322202646295	28.03950290601522
>50	0.11587896560286623	2.4407619975356116
>100	0.07212874389566164	4.119671851089892
>500	0.008277068971633302	1.5873131263198832
>1k	0.010641945820671388	8.4079979935955
>5k	0.0023648768490380865	6.089364965705749
>10k+	0.0011824384245190432	4.031709914618765
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC	11092	4.031709914618765	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG	9410	3.420338108236799	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC	7343	2.6690268574689497	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	4451	1.617845368731349	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAAGAT	3821	1.388853550645357	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	2896	1.0526354050429088	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTA	2306	0.8381827500099956	No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	2139	0.7774817442633914	Illumina Single End Adapter 1 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT	2050	0.745132106470291	No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1851	0.6727997702812237	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTC	1850	0.6724362912048968	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCT	1768	0.6426310069460851	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGT	788	0.28642151214565337	No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	749	0.27224582816890147	No Hit
TGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT	692	0.25152752081826407	Illumina Single End Adapter 1 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATG	626	0.22753790178068398	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTAT	507	0.18428389169777443	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTT	503	0.18282997539246654	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	502	0.18246649631613956	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCAGCAAGA	492	0.17883170555286987	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCCAGATCGTATGCCGTCTTCTGC	449	0.16320210527081008	Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	423	0.15375164928630883	No Hit
TGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	396	0.14393771422548063	No Hit
TTCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT	354	0.12867159301974782	No Hit
GGGCAAACTGAAGTAGTGATGATAAAAAAGTTAAACAAACAAACAAACAAA	351	0.1275811557907669	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCCAGATCGTATGCCGTCTTCTGC	325	0.11813069980626564	Illumina Single End Adapter 1 (95% over 21bp)
TTTTTTGGGCATGTTGCTTTTGGAATCAGCCTGACTCTTTGCCCAGTATAT	313	0.11376895089034199	No Hit
TTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	309	0.1123150345850341	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC	286	0.10395501582951379	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9468629938317601	0.0
2	0.0	0.0	0.0	4.9524024149549835	0.0
3	0.0	0.0	0.0	6.121351124422523	0.0
4	0.0	0.0	0.0	8.248794158164285	0.0
5	0.0	0.0	0.0	13.065255398572981	0.0
6	0.0	0.0	0.0	14.265463308604641	0.0
7	0.0	0.0	0.0	15.881491281954354	0.0
8	0.0	0.0	0.0	17.917701067538047	0.0
9	0.0	0.0	0.0	18.481820593997508	0.0
10	0.0	0.0	0.0	23.372431565976903	0.0
11	0.0	0.0	0.0	25.509688534779496	0.0
12	0.0	0.0	0.0	31.781519996801386	0.0
13	0.0	0.0	0.0	32.76582133549482	0.0
14	0.0	0.0	0.0	33.190364896644724	0.0
15	0.0	0.0	0.0	34.48871215728467	0.0
16	0.0	0.0	0.0	35.617678168356235	0.0
17	0.0	0.0	0.0	36.91566194991985	0.0
18	0.0	0.0	0.0	38.17329955401117	0.0
19	0.0	0.0	0.0	40.59443368142513	0.0
20	0.0	0.0	0.0	41.68741526394033	0.0
21	0.0	0.0	0.0	42.83746306143887	0.0
22	0.0	0.0	0.0	44.132539010391866	0.0
23	0.0	0.0	0.0	45.23315365350994	0.0
24	0.0	0.0	0.0	46.0749711942832	0.0
25	0.0	0.0	0.0	46.81973982167717	0.0
26	0.0	0.0	0.0	47.57577630043726	0.0
27	3.634790763269712E-4	0.0	0.0	48.27511004329036	0.0
28	3.634790763269712E-4	0.0	0.0	48.962812455700984	0.0
29	3.634790763269712E-4	0.0	0.0	49.67305057084389	0.0
30	3.634790763269712E-4	0.0	0.0	50.452713189565245	0.0
31	7.269581526539424E-4	0.0	0.0	51.15713563948691	0.0
32	7.269581526539424E-4	0.0	0.0	51.84629196820285	0.0
33	7.269581526539424E-4	0.0	0.0	52.49582907759915	0.0
34	7.269581526539424E-4	0.0	0.0	53.094115637233344	0.0
35	7.269581526539424E-4	0.0	0.0	53.72438835558431	0.0
36	7.269581526539424E-4	0.0	0.0	54.39246289787329	0.0
37	7.269581526539424E-4	0.0	0.0	54.994747727347075	0.0
38	7.269581526539424E-4	0.0	0.0	55.620295217705795	0.0
39	7.269581526539424E-4	0.0	0.0	56.2389366056143	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTGCG	30	2.1594624E-6	45.000004	24
GGAGTTC	35	1.207427E-7	45.000004	8
TTGCGAC	30	2.1594624E-6	45.000004	26
CAGCTAT	35	1.207427E-7	45.000004	16
GGAATGC	25	3.883046E-5	45.0	8
TCGTTTG	55	1.8189894E-12	45.0	1
TGATAAG	20	7.0239237E-4	45.0	26
CCGGGTG	20	7.0239237E-4	45.0	5
ACCGGTG	20	7.0239237E-4	45.0	17
ATTAGCG	20	7.0239237E-4	45.0	15
GGTAAGT	20	7.0239237E-4	45.0	9
GTCGACT	20	7.0239237E-4	45.0	12
TTAGCGT	20	7.0239237E-4	45.0	16
TTTGTCG	50	2.1827873E-11	45.0	1
TTCACGA	20	7.0239237E-4	45.0	32
CCCAGTT	20	7.0239237E-4	45.0	13
CACGATA	20	7.0239237E-4	45.0	34
TATGCGG	20	7.0239237E-4	45.0	2
ACGGGAT	25	3.883046E-5	45.0	5
GACTTTA	20	7.0239237E-4	45.0	41
>>END_MODULE