##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933785.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	839316
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.941601256261052	31.0	25.0	33.0	16.0	34.0
2	29.445988161788886	31.0	28.0	34.0	16.0	34.0
3	30.318879897440297	31.0	30.0	34.0	25.0	34.0
4	33.23223315175691	35.0	32.0	37.0	28.0	37.0
5	34.01861754095001	35.0	33.0	37.0	28.0	37.0
6	33.65399325164777	35.0	35.0	37.0	28.0	37.0
7	33.52965629155169	35.0	32.0	35.0	29.0	37.0
8	34.24729899108321	35.0	35.0	37.0	31.0	37.0
9	34.98292657354322	37.0	34.0	39.0	29.0	39.0
10	33.71655252610459	35.0	31.0	39.0	27.0	39.0
11	33.96232170005099	35.0	32.0	39.0	27.0	39.0
12	34.28605674144184	37.0	32.0	39.0	27.0	39.0
13	34.70306892755529	37.0	34.0	39.0	27.0	39.0
14	34.5078123138365	37.0	32.0	39.0	25.0	40.0
15	35.022085841327936	37.0	32.0	39.0	27.0	40.0
16	34.8229379637705	37.0	32.0	39.0	27.0	40.0
17	34.673952361208414	36.0	33.0	39.0	27.0	40.0
18	34.584614138179184	37.0	32.0	39.0	27.0	40.0
19	34.43169557115556	37.0	32.0	39.0	25.0	40.0
20	35.62343265230259	37.0	34.0	39.0	30.0	40.0
21	34.66389417096779	37.0	33.0	39.0	25.0	40.0
22	35.53137793155379	37.0	34.0	39.0	28.0	40.0
23	36.4970332985431	38.0	35.0	40.0	31.0	41.0
24	36.57763941113955	38.0	35.0	40.0	31.0	41.0
25	34.28611631376025	37.0	32.0	39.0	24.0	40.0
26	34.972226193710114	37.0	33.0	39.0	27.0	40.0
27	35.74882165954182	37.0	34.0	39.0	30.0	40.0
28	35.96363229105605	38.0	34.0	40.0	30.0	41.0
29	36.514625004170064	38.0	35.0	40.0	30.0	41.0
30	34.11463977810503	37.0	32.0	39.0	24.0	40.0
31	35.00691634616759	37.0	33.0	39.0	27.0	40.0
32	35.46912724170634	37.0	34.0	39.0	30.0	40.0
33	36.46005556905862	38.0	35.0	40.0	31.0	41.0
34	36.78492486739202	38.0	35.0	40.0	31.0	41.0
35	36.90586263099953	39.0	35.0	40.0	32.0	41.0
36	36.81600374590738	39.0	35.0	40.0	31.0	41.0
37	37.06921469386977	39.0	35.0	40.0	32.0	41.0
38	36.62328372150656	38.0	35.0	40.0	31.0	41.0
39	36.5199531523288	38.0	35.0	40.0	31.0	41.0
40	36.15542894452149	38.0	35.0	40.0	30.0	41.0
41	35.82799803649638	38.0	34.0	40.0	30.0	41.0
42	36.54823689766429	38.0	35.0	40.0	31.0	41.0
43	36.533154378088824	38.0	35.0	40.0	31.0	41.0
44	36.888735589456175	39.0	35.0	40.0	32.0	41.0
45	37.10608400173474	39.0	35.0	40.0	33.0	41.0
46	36.84195940503934	39.0	35.0	40.0	32.0	41.0
47	36.812203031992716	39.0	35.0	40.0	32.0	41.0
48	36.788456314427464	39.0	35.0	40.0	32.0	41.0
49	36.932939441163995	39.0	35.0	40.0	32.0	41.0
50	36.803688956245324	39.0	35.0	40.0	32.0	41.0
51	36.0218058514314	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	23.0
11	15.0
12	27.0
13	31.0
14	64.0
15	61.0
16	112.0
17	239.0
18	372.0
19	579.0
20	904.0
21	1269.0
22	1918.0
23	2581.0
24	3603.0
25	5080.0
26	6824.0
27	9032.0
28	12170.0
29	16713.0
30	23369.0
31	33016.0
32	46468.0
33	62139.0
34	79054.0
35	107413.0
36	143178.0
37	162284.0
38	107547.0
39	13222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.61907553293396	4.382378031635284	53.07321676222067	16.92532967321009
2	19.307984120402804	7.1462953166626155	52.89986131564274	20.645859247291842
3	20.774892888971497	7.280571322362494	50.766100014773926	21.178435773892073
4	18.113797425522687	4.5413169771575905	52.48642942586582	24.8584561714539
5	20.41019115565532	5.648289797883038	49.28417902196551	24.65734002449614
6	21.77118034208808	8.781436312425832	51.716278493439894	17.73110485204619
7	82.67672723980003	1.8923742666647603	11.405716083096236	4.025182410438976
8	83.69017152061917	4.740645954563001	6.744063022747095	4.825119502070733
9	78.11968317058175	4.936758026774183	8.470945388864266	8.472613413779792
10	35.7259959300192	30.253563616087387	17.82379937949473	16.196641074398677
11	29.84084659413141	25.29166607094348	24.973073312077933	19.894414022847176
12	26.96993742523674	21.89604392147892	29.396317954143612	21.73770069914073
13	24.474572151609166	22.473776265435188	32.395069318349705	20.65658226460594
14	21.762482783599978	24.838678161741225	30.182672557177508	23.216166497481282
15	19.20301769536146	25.234714934541934	34.174375324669136	21.387892045427467
16	21.668596809783207	24.719056946370614	30.373661410005294	23.23868483384089
17	21.99409995758451	23.918285842281094	31.077925358267922	23.00968884186647
18	21.324983677184754	23.88897626162256	31.605497810121573	23.18054225107111
19	22.932602261841787	25.611926854724558	28.981575473361644	22.47389541007201
20	24.942453140414337	25.506483851135926	30.685343779935092	18.865719228514646
21	25.131773968326588	25.880478866124317	28.231798273832503	20.75594889171659
22	23.17911251542923	23.790562791606497	29.53047481520667	23.499849877757605
23	22.293510429921508	26.245776322624614	28.783437942324465	22.677275305129417
24	22.80142401669931	24.540101701861992	28.60698473518913	24.051489546249567
25	21.25707123419546	26.538514695299504	26.75416648794971	25.45024758255532
26	20.796458068236518	27.499058757369095	28.434105867158493	23.270377307235893
27	21.894018462652923	27.66609953819539	27.6278541097751	22.812027889376587
28	20.342397857302853	26.665522878153165	29.8929128004232	23.099166464120785
29	21.40635946413508	26.874264281867617	28.601384937258434	23.117991316738866
30	22.033775121646674	26.14652884015079	29.209618308241474	22.610077729961063
31	23.688336693212094	27.55779706332299	26.33251361823199	22.421352625232927
32	23.498896720663016	29.457796586744443	25.93111533677423	21.11219135581831
33	22.324011456948277	25.631109141253116	27.313550557835192	24.731328843963418
34	23.45278774621239	26.933360021731982	27.52789175948034	22.085960472575287
35	21.51978515839088	27.624398915307225	26.77215732811003	24.083658598191864
36	22.244184550276653	29.06044922293868	25.89310819762759	22.802258029157073
37	22.357371955258806	28.86040537771233	27.579600531861658	21.202622135167207
38	23.12371025930639	27.20453321514185	26.74201373499373	22.929742790558024
39	22.785577780001812	26.538157261389035	28.314722941061532	22.361542017547624
40	23.55072463768116	24.419169895486327	30.117143006924685	21.91296245990783
41	22.910083925482176	25.795886173979767	27.080265358935137	24.213764541602924
42	22.693002397190092	25.33038807791106	26.970533148420856	25.00607637647799
43	22.230125483131502	25.542822965367034	26.78824185408118	25.43880969742028
44	21.650725114259707	25.24245933593545	28.00911694760972	25.097698602195123
45	22.142792464340012	24.31229715625581	27.62749667586463	25.917413703539548
46	22.764489179284084	26.837448589089213	27.628092399048747	22.769969832577956
47	20.114474167059843	26.10661538681498	30.93566666190088	22.843243784224295
48	21.87698077958719	25.683175347544907	28.524655791144216	23.91518808172369
49	21.912605025997358	24.177425427371812	31.09591619842824	22.814053348202584
50	22.600427014378376	23.49651382792655	28.65249798645564	25.25056117123944
51	21.245156770513134	23.25417363662792	27.68706899427629	27.813600598582656
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	120.0
1	492.5
2	865.0
3	1634.5
4	2404.0
5	1844.0
6	1284.0
7	1263.0
8	1242.0
9	1303.5
10	1365.0
11	1382.5
12	1400.0
13	1405.5
14	1411.0
15	1405.5
16	1400.0
17	1360.0
18	1320.0
19	1619.5
20	1919.0
21	2406.5
22	2894.0
23	3224.0
24	3554.0
25	4359.0
26	6481.0
27	7798.0
28	8982.5
29	10167.0
30	12033.0
31	13899.0
32	16132.0
33	18365.0
34	20784.0
35	23203.0
36	25562.0
37	27921.0
38	30865.5
39	33810.0
40	37031.0
41	40252.0
42	44127.0
43	48002.0
44	53449.0
45	58896.0
46	75425.5
47	91955.0
48	89350.0
49	86745.0
50	84296.0
51	81847.0
52	70709.0
53	59571.0
54	52449.0
55	45327.0
56	41966.0
57	38605.0
58	36037.0
59	33469.0
60	31069.0
61	28669.0
62	25082.0
63	21495.0
64	19168.0
65	16841.0
66	13129.5
67	9418.0
68	7892.5
69	6367.0
70	5196.0
71	4025.0
72	3504.0
73	2983.0
74	2338.5
75	1233.0
76	772.0
77	605.0
78	438.0
79	334.0
80	230.0
81	192.0
82	154.0
83	95.0
84	36.0
85	23.0
86	10.0
87	7.5
88	5.0
89	3.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	839316.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.62663128599471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.14556155254701	22.09575512590893
2	11.111390499362262	6.806089197973453
3	4.159048291542692	3.821329155771727
4	1.9532086592070224	2.3928080572058223
5	1.1725677534347323	1.795590012114635
6	0.7518674578815374	1.38163004450856
7	0.5810967915455592	1.2457926023298256
8	0.45535440651601644	1.1156777210255184
9	0.4249774430937399	1.1714064709047094
>10	7.045890835222861	47.99802032435134
>50	0.14740510917449648	2.9169364186419426
>100	0.044536837048459725	2.3725381552709797
>500	0.001970656506569014	0.3457109782089079
>1k	0.004335444314451831	2.906072555998414
>5k	7.882626026276056E-4	1.634643179785276
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC	7457	0.8884615567914826	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG	6085	0.7249951150698902	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	4549	0.5419889529092737	No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	3789	0.45143902892355203	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	3176	0.3784033665508581	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGATGGCT	2329	0.2774878591615077	No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	2227	0.26533510620552925	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	2028	0.241625323477689	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTA	1497	0.17835952132450708	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	1310	0.15607947423854662	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCT	1061	0.12641245966954043	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1058	0.1260550257590705	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTC	1051	0.12522101330130725	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.32407341215942503	0.0
2	0.0	0.0	0.0	1.554718365907477	0.0
3	0.0	0.0	0.0	1.9792307069089592	0.0
4	0.0	0.0	0.0	2.6743205181361964	0.0
5	0.0	0.0	0.0	4.095001167617441	0.0
6	0.0	0.0	0.0	4.605535936405358	0.0
7	0.0	0.0	0.0	5.227828374533549	0.0
8	0.0	0.0	0.0	6.09651192161236	0.0
9	0.0	0.0	0.0	6.415104680477913	0.0
10	0.0	0.0	0.0	7.879511411673315	0.0
11	0.0	0.0	0.0	9.210952728173893	0.0
12	0.0	0.0	0.0	11.313140700284517	0.0
13	0.0	0.0	0.0	11.778877085626867	0.0
14	0.0	0.0	0.0	11.93876918824376	0.0
15	0.0	0.0	0.0	12.358277454498664	0.0
16	0.0	0.0	0.0	12.926835661419537	0.0
17	0.0	0.0	0.0	13.717360326742252	0.0
18	0.0	0.0	0.0	14.504310652960267	0.0
19	0.0	0.0	0.0	15.366441245013798	0.0
20	0.0	0.0	0.0	16.022451615362986	0.0
21	0.0	0.0	0.0	16.8998327209299	0.0
22	0.0	0.0	0.0	17.870742366403118	0.0
23	0.0	0.0	0.0	18.762182539115184	0.0
24	0.0	0.0	0.0	19.459178664531596	0.0
25	0.0	0.0	0.0	20.111376406502437	0.0
26	0.0	0.0	0.0	20.714963136649367	0.0
27	0.0	0.0	0.0	21.29758041071539	0.0
28	0.0	0.0	0.0	21.88961011109046	0.0
29	0.0	0.0	0.0	22.517859781059816	0.0
30	0.0	0.0	0.0	23.168746932025602	0.0
31	0.0	0.0	0.0	23.825591314832554	0.0
32	0.0	0.0	0.0	24.437399024920293	0.0
33	0.0	0.0	0.0	25.016322815244795	0.0
34	0.0	0.0	0.0	25.607161069251628	0.0
35	0.0	0.0	0.0	26.211343522582673	0.0
36	0.0	0.0	0.0	26.83673372126827	0.0
37	0.0	0.0	0.0	27.43889071577332	0.0
38	0.0	0.0	0.0	28.074169919315253	0.0
39	0.0	0.0	0.0	28.690862559512745	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTACG	20	7.032199E-4	45.0	1
ATCCGAA	20	7.032199E-4	45.0	10
ACTACGC	25	3.8899037E-5	45.0	34
CCGACTA	25	3.8899037E-5	45.0	26
TACCGCT	20	7.032199E-4	45.0	12
TGTTCCG	80	0.0	42.1875	1
TACGGCT	950	0.0	41.210526	7
CGTTTGG	580	0.0	41.12069	2
ACATACG	55	6.002665E-11	40.909092	17
TTCACGG	50	1.0804797E-9	40.5	2
TGGGCGA	1075	0.0	40.395348	6
CGGTAGG	130	0.0	39.807693	31
TTTAGCG	40	3.4567347E-7	39.375	1
AACACGT	40	3.4567347E-7	39.375	41
ATACGGC	1890	0.0	39.285713	6
TGATACG	1905	0.0	39.094486	4
GGCGATA	75	0.0	39.0	8
TTTGGGA	4925	0.0	38.92386	4
GATACGG	1915	0.0	38.772846	5
CGTCGCG	35	6.246275E-6	38.57143	15
>>END_MODULE