SRR2933784.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933784.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620476
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGC	18579	2.994314042767166	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCG	15148	2.4413514785422805	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	12286	1.9800927030215514	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGGAGCTT	5485	0.8839987364539482	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	5060	0.8155029364552375	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTA	3692	0.5950270437535053	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT	3685	0.5938988776358796	No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	2962	0.47737543434395524	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTC	2818	0.4541674456385098	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCT	2633	0.4243516268155416	No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	2438	0.3929241421102509	No Hit
TCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	1792	0.28881052611221064	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGT	1288	0.20758256564315142	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATG	1180	0.19017657411406727	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1166	0.18792024187881562	No Hit
TGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCTT	904	0.14569459576196342	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTAT	813	0.1310284362328277	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAAGGAGCT	714	0.11507294399783392	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG	704	0.11346127811551132	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	20	7.0307753E-4	45.0	13
ACGTTAG	25	3.888723E-5	45.0	31
GCGAACC	20	7.0307753E-4	45.0	33
TTTCGCA	20	7.0307753E-4	45.0	14
CCGTATA	20	7.0307753E-4	45.0	29
TACGAAC	20	7.0307753E-4	45.0	9
TACGGCT	2245	0.0	43.596878	7
GGCGACT	540	0.0	42.916668	10
ACGGCTG	2285	0.0	42.8337	8
TTGTGCG	95	0.0	42.63158	1
GCGACTG	535	0.0	42.476635	11
GATACCT	1900	0.0	41.92105	5
TGATACC	1915	0.0	41.59269	4
ATGATAC	6550	0.0	41.049618	3
ATACGGC	4675	0.0	40.90909	6
AATGATA	6515	0.0	40.890255	2
TGATACG	4700	0.0	40.69149	4
GATACGG	4720	0.0	40.614407	5
GAATGAT	6880	0.0	40.552326	1
GTTGGGA	805	0.0	40.52795	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content