##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933784.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620476
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.07229288481746	31.0	16.0	33.0	16.0	34.0
2	28.21942830987822	31.0	25.0	33.0	16.0	34.0
3	29.118536413978944	31.0	28.0	34.0	16.0	34.0
4	32.51037751661627	35.0	30.0	37.0	27.0	37.0
5	33.736068760113206	35.0	33.0	37.0	28.0	37.0
6	32.891845937634976	35.0	32.0	37.0	26.0	37.0
7	33.382880240331616	35.0	32.0	35.0	28.0	37.0
8	33.41688155545098	35.0	33.0	37.0	28.0	37.0
9	34.71445470896538	37.0	32.0	39.0	28.0	39.0
10	33.455906433125534	35.0	31.0	38.0	27.0	39.0
11	33.95684281100316	35.0	32.0	39.0	27.0	39.0
12	34.30196494304373	37.0	32.0	39.0	27.0	39.0
13	34.658062519742906	37.0	34.0	39.0	27.0	39.0
14	34.56810900018695	37.0	33.0	39.0	26.0	40.0
15	35.012865928738584	37.0	33.0	39.0	27.0	40.0
16	34.74703936977417	36.0	32.0	39.0	27.0	40.0
17	34.46856123363353	36.0	32.0	39.0	27.0	40.0
18	34.46625010475828	36.0	32.0	39.0	27.0	40.0
19	34.26691926843262	37.0	32.0	39.0	26.0	40.0
20	35.030769925025304	37.0	33.0	39.0	27.0	40.0
21	34.48844113229198	37.0	32.0	39.0	25.0	40.0
22	35.38271907374338	37.0	34.0	39.0	28.0	40.0
23	36.15909237424171	38.0	34.0	40.0	30.0	40.0
24	36.325579071551516	38.0	35.0	40.0	31.0	41.0
25	34.18197319477304	37.0	32.0	39.0	24.0	40.0
26	34.94072615217994	37.0	33.0	39.0	27.0	40.0
27	35.48051173615095	37.0	34.0	39.0	30.0	40.0
28	35.620049768242446	38.0	34.0	40.0	29.0	40.0
29	36.138886919075034	38.0	35.0	40.0	30.0	41.0
30	33.67091394348855	37.0	31.0	39.0	23.0	40.0
31	34.743957864607175	37.0	33.0	39.0	26.0	40.0
32	35.196218709506894	37.0	34.0	39.0	29.0	40.0
33	35.97577021512516	38.0	35.0	40.0	30.0	40.0
34	36.32020577749986	38.0	35.0	40.0	30.0	41.0
35	36.45436245721027	38.0	35.0	40.0	31.0	41.0
36	36.383080086901025	38.0	35.0	40.0	30.0	41.0
37	36.633038183588084	39.0	35.0	40.0	31.0	41.0
38	36.15441693151709	38.0	35.0	40.0	30.0	41.0
39	36.06025212901063	38.0	35.0	40.0	30.0	41.0
40	35.63455798451511	38.0	34.0	40.0	29.0	41.0
41	35.32998858940555	37.0	34.0	40.0	28.0	41.0
42	36.1976949954551	38.0	35.0	40.0	31.0	41.0
43	36.23869577550139	38.0	35.0	40.0	31.0	41.0
44	36.675637736189636	39.0	35.0	40.0	32.0	41.0
45	36.92473520329553	39.0	35.0	40.0	33.0	41.0
46	36.62916857380463	39.0	35.0	40.0	32.0	41.0
47	36.52582694576422	38.0	35.0	40.0	31.0	41.0
48	36.53618028739226	38.0	35.0	40.0	32.0	41.0
49	36.734627930814405	39.0	35.0	40.0	32.0	41.0
50	36.570170320850444	39.0	35.0	40.0	31.0	41.0
51	35.97022930782174	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	8.0
11	19.0
12	6.0
13	17.0
14	27.0
15	50.0
16	84.0
17	206.0
18	439.0
19	815.0
20	1297.0
21	1734.0
22	2141.0
23	2537.0
24	3049.0
25	4034.0
26	5729.0
27	7652.0
28	10108.0
29	14052.0
30	19744.0
31	27278.0
32	38353.0
33	50270.0
34	63909.0
35	82847.0
36	101504.0
37	107648.0
38	67155.0
39	7754.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.613632114699037	4.043830865335646	47.517228708282026	16.825308311683287
2	17.786989343665187	16.595323590275854	46.67642261747433	18.941264448584636
3	18.57735029235619	16.52876178933593	46.10685989466152	18.787028023646364
4	18.01745756483732	3.810783978751797	56.061314216827085	22.110444239583803
5	26.586846227734835	4.939272429554084	44.15851700952172	24.315364333189358
6	19.81430385703879	15.637027056646833	47.108026740760316	17.440642345554057
7	73.48841856896962	1.7697703053784513	20.07539372997505	4.666417395676867
8	72.56590101792817	11.609635183310878	9.177631366886068	6.646832431874882
9	67.95025109754447	4.186463295921196	10.4197422623921	17.44354334414224
10	40.06988183265751	23.057942611801263	19.25763446128456	17.614541094256666
11	30.904660293065323	23.136753073446837	26.082878306332557	19.87570832715528
12	25.597122209400524	19.099530038228714	30.218090627195892	25.085257125174863
13	23.88859520755033	20.737627241021407	35.89727886332429	19.476498688103973
14	21.79085089511923	25.941857541629332	27.98093721594389	24.28635434730755
15	16.75407267968463	23.69100497037758	37.65383350846769	21.9010888414701
16	18.5359304791805	23.77368343014073	29.065104854982305	28.625281235696466
17	18.69870873329508	25.46125877552073	32.409311560801704	23.43072093038248
18	18.761724869293896	23.693905968965762	31.715328231873595	25.829040929866746
19	20.363720756322564	25.33587116987603	28.897008103456056	25.403399970345347
20	22.958180493685493	25.239977049877837	34.28754698006047	17.5142954763762
21	23.993836989665997	28.14194263758792	28.313423887467042	19.550796485279044
22	20.449300214673897	22.508848045693952	30.976057091652216	26.06579464797994
23	21.2796304772465	27.899709255474832	28.04717668370735	22.773483583571323
24	22.73834926733669	23.235709358621445	27.37156634583771	26.654375028204154
25	18.870189983174207	30.193109806019898	25.021596322823125	25.915103887982777
26	19.578194805278528	25.92912538115898	29.012403380630353	25.480276432932136
27	22.067251593937556	29.013531546747977	25.32861867340558	23.590598185908885
28	18.643912093296112	26.977675204198064	31.380585228115187	22.99782747439063
29	23.630728666378715	25.89270173221849	26.385549159032745	24.091020442370052
30	21.320405624069263	29.85272597167336	27.589785906304193	21.237082497953182
31	27.284697554780525	25.00257866541172	23.693905968965762	24.018817810842
32	25.608403870576783	29.92944126767192	22.844880382158212	21.61727447959309
33	21.750236914884702	26.511742597618603	25.114428277644908	26.623592209851793
34	26.754137146319923	25.543614902107414	25.15326942540888	22.548978526163786
35	20.302638619382538	26.8924180790232	25.15069075999716	27.654252541597096
36	22.736576434866134	30.192303973078733	25.900921228218337	21.170198363836796
37	24.017689644724374	27.80204230300608	28.07231867147158	20.10794938079797
38	26.008902842333946	26.56299357267646	23.73435878261206	23.693744802377527
39	28.858005789103846	24.17450473507436	26.756393478555175	20.211095997266614
40	21.172938195836743	26.959141046551355	27.724198840889898	24.143721916722
41	23.70099729884798	25.07961629458674	27.456501137836113	23.76288526872917
42	20.701687091845617	22.313352974168218	29.87335529496709	27.111604639019077
43	22.525609370870107	23.892624372256137	29.15455231145121	24.427213945422547
44	22.431326916754234	23.839278231551262	29.22014711286174	24.50924773883277
45	22.786376910629905	21.548134013241445	26.35251000844513	29.31297906768352
46	27.057452665373038	24.9845280075297	26.17297042915439	21.78504889794287
47	18.13736550648212	24.11567893036959	36.11839942237895	21.628556140769344
48	23.05987661086005	24.6647734964769	27.026669847020674	25.24868004564238
49	20.81530953654936	21.14779620807251	33.4473855556057	24.589508699772434
50	23.466661079558275	21.19695201748335	29.163577640392212	26.17280926256616
51	20.81095803866709	21.489308208536674	26.546232247500306	31.15350150529593
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	96.0
1	444.0
2	792.0
3	1621.0
4	2450.0
5	1787.5
6	1125.0
7	1130.5
8	1136.0
9	1087.0
10	1038.0
11	1070.0
12	1102.0
13	1122.0
14	1142.0
15	1095.0
16	1048.0
17	1035.5
18	1023.0
19	1218.0
20	1413.0
21	1317.0
22	1221.0
23	1756.5
24	2292.0
25	2490.0
26	3778.0
27	4868.0
28	5639.5
29	6411.0
30	7531.5
31	8652.0
32	9590.5
33	10529.0
34	12436.5
35	14344.0
36	15832.5
37	17321.0
38	18248.5
39	19176.0
40	21639.0
41	24102.0
42	26601.5
43	29101.0
44	32775.5
45	36450.0
46	72283.5
47	108117.0
48	89728.5
49	71340.0
50	66592.5
51	61845.0
52	52121.5
53	42398.0
54	36865.5
55	31333.0
56	28697.5
57	26062.0
58	25396.5
59	24731.0
60	22958.5
61	21186.0
62	18078.0
63	14970.0
64	12893.5
65	10817.0
66	8661.0
67	6505.0
68	5397.0
69	4289.0
70	3651.0
71	3013.0
72	2458.0
73	1903.0
74	1533.5
75	940.0
76	716.0
77	528.0
78	340.0
79	241.5
80	143.0
81	104.0
82	65.0
83	39.5
84	14.0
85	8.5
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	620476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.938617362766138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.59240263230384	20.71785145857332
2	11.519241286806055	6.667018318165166
3	4.316361706461205	3.747286194677313
4	2.144514648951464	2.482371554194127
5	1.2655647913241674	1.8311847621959527
6	0.8461329099435408	1.4691549911339994
7	0.6216422538113194	1.2592627123701328
8	0.5049078860726592	1.1689068894799843
9	0.4430185993759778	1.1538311158737007
>10	6.551054830593508	40.38317755925283
>50	0.12747344128475815	2.455080629628812
>100	0.055840135784030936	2.893178921625592
>500	0.0033842506535776327	0.6790197074167821
>1k	0.005640417755962721	3.860945230585712
>5k	0.0011280835511925443	1.7212170227668189
>10k+	0.0016921253267888163	7.51051293205971
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGC	18579	2.994314042767166	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCG	15148	2.4413514785422805	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	12286	1.9800927030215514	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGGAGCTT	5485	0.8839987364539482	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	5060	0.8155029364552375	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTA	3692	0.5950270437535053	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT	3685	0.5938988776358796	No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	2962	0.47737543434395524	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTC	2818	0.4541674456385098	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCT	2633	0.4243516268155416	No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	2438	0.3929241421102509	No Hit
TCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	1792	0.28881052611221064	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGT	1288	0.20758256564315142	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATG	1180	0.19017657411406727	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1166	0.18792024187881562	No Hit
TGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCTT	904	0.14569459576196342	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTAT	813	0.1310284362328277	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAAGGAGCT	714	0.11507294399783392	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG	704	0.11346127811551132	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4411129519916967	0.0
2	0.0	0.0	0.0	2.3132240408976332	0.0
3	0.0	0.0	0.0	2.8636079397108025	0.0
4	0.0	0.0	0.0	4.197906123685686	0.0
5	0.0	0.0	0.0	7.373532578214145	0.0
6	0.0	0.0	0.0	8.015781432319702	0.0
7	0.0	0.0	0.0	9.052082594653138	0.0
8	0.0	0.0	0.0	10.354630960746265	0.0
9	0.0	0.0	0.0	10.728053945680413	0.0
10	0.0	0.0	0.0	14.091117142322991	0.0
11	0.0	0.0	0.0	15.621393897588304	0.0
12	0.0	0.0	0.0	20.167742185032136	0.0
13	0.0	0.0	0.0	20.84673702125465	0.0
14	0.0	0.0	0.0	21.12990671677873	0.0
15	0.0	0.0	0.0	22.03308427723232	0.0
16	0.0	0.0	0.0	22.82441222545272	0.0
17	0.0	0.0	0.0	23.6631231506134	0.0
18	0.0	0.0	0.0	24.502801075303477	0.0
19	0.0	0.0	0.0	26.09754446586169	0.0
20	0.0	0.0	0.0	26.912402735964	0.0
21	0.0	0.0	0.0	27.809617132652996	0.0
22	1.611665882322604E-4	0.0	0.0	28.84382312933941	0.0
23	1.611665882322604E-4	0.0	0.0	29.716701371205332	0.0
24	1.611665882322604E-4	0.0	0.0	30.419065362721522	0.0
25	1.611665882322604E-4	0.0	0.0	31.05825205165067	0.0
26	1.611665882322604E-4	0.0	0.0	31.62878177399287	0.0
27	1.611665882322604E-4	0.0	0.0	32.181583171629526	0.0
28	1.611665882322604E-4	0.0	0.0	32.7604935565598	0.0
29	1.611665882322604E-4	0.0	0.0	33.33956510807831	0.0
30	1.611665882322604E-4	0.0	0.0	33.95892830665489	0.0
31	1.611665882322604E-4	0.0	0.0	34.56604284452582	0.0
32	1.611665882322604E-4	0.0	0.0	35.15575139086766	0.0
33	1.611665882322604E-4	0.0	0.0	35.70710228921022	0.0
34	1.611665882322604E-4	0.0	0.0	36.25394052308228	0.0
35	1.611665882322604E-4	0.0	0.0	36.819151748012814	0.0
36	1.611665882322604E-4	0.0	0.0	37.39967379882542	0.0
37	1.611665882322604E-4	0.0	0.0	37.957632527285504	0.0
38	1.611665882322604E-4	0.0	0.0	38.53783224492164	0.0
39	1.611665882322604E-4	0.0	0.0	39.0901501427936	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	20	7.0307753E-4	45.0	13
ACGTTAG	25	3.888723E-5	45.0	31
GCGAACC	20	7.0307753E-4	45.0	33
TTTCGCA	20	7.0307753E-4	45.0	14
CCGTATA	20	7.0307753E-4	45.0	29
TACGAAC	20	7.0307753E-4	45.0	9
TACGGCT	2245	0.0	43.596878	7
GGCGACT	540	0.0	42.916668	10
ACGGCTG	2285	0.0	42.8337	8
TTGTGCG	95	0.0	42.63158	1
GCGACTG	535	0.0	42.476635	11
GATACCT	1900	0.0	41.92105	5
TGATACC	1915	0.0	41.59269	4
ATGATAC	6550	0.0	41.049618	3
ATACGGC	4675	0.0	40.90909	6
AATGATA	6515	0.0	40.890255	2
TGATACG	4700	0.0	40.69149	4
GATACGG	4720	0.0	40.614407	5
GAATGAT	6880	0.0	40.552326	1
GTTGGGA	805	0.0	40.52795	4
>>END_MODULE