SRR2933783.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933783.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	230125
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGC	8836	3.839652362846279	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCG	7796	3.3877240630092342	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	7299	3.1717544812601846	RNA PCR Primer, Index 41 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	4488	1.95024443237371	Illumina PCR Primer Index 11 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	3395	1.4752851711026616	RNA PCR Primer, Index 41 (96% over 26bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGACATCT	2979	1.2945138511678436	No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	2583	1.1224334600760455	RNA PCR Primer, Index 41 (96% over 27bp)
GAACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	2419	1.0511678435632807	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGACATCTCGTA	2027	0.8808256382400869	No Hit
TCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	1823	0.7921781640412819	Illumina PCR Primer Index 11 (96% over 25bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTC	1712	0.7439435089625204	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCT	1681	0.7304725692558393	RNA PCR Primer, Index 41 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCTT	995	0.4323737099402499	Illumina PCR Primer Index 11 (96% over 27bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCGACATCTCGT	661	0.2872351982618142	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	643	0.27941336230309616	RNA PCR Primer, Index 41 (96% over 25bp)
GAATGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTT	612	0.265942422596415	RNA PCR Primer, Index 41 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATG	611	0.26550787615426397	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	455	0.19771863117870725	Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	455	0.19771863117870725	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTAT	453	0.1968495382944052	No Hit
TGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	404	0.17555676262900596	Illumina PCR Primer Index 11 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	389	0.1690385659967409	RNA PCR Primer, Index 41 (96% over 25bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCGACATC	387	0.1681694731124389	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	328	0.14253123302552959	Illumina PCR Primer Index 11 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	293	0.12732210755024442	Illumina PCR Primer Index 11 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCC	277	0.12036936447582834	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGC	271	0.11776208582292233	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT	233	0.10124932102118414	RNA PCR Primer, Index 41 (95% over 24bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTTGC	35	1.2062628E-7	45.000004	18
TCGGGCA	35	1.2062628E-7	45.000004	5
CGGGCGA	45	3.8198777E-10	45.000004	6
CTTTGCG	35	1.2062628E-7	45.000004	1
CGGGATC	70	0.0	45.000004	6
TTGCGCG	35	1.2062628E-7	45.000004	1
CGGGAGT	45	3.8198777E-10	45.000004	6
GGGCCGA	35	1.2062628E-7	45.000004	7
TTACGGG	35	1.2062628E-7	45.000004	3
CTGATAC	35	1.2062628E-7	45.000004	3
CTTGTCG	25	3.881053E-5	45.0	1
CAGCGTC	20	7.021517E-4	45.0	36
CGGAATC	20	7.021517E-4	45.0	41
AGCGTCT	20	7.021517E-4	45.0	37
CCGGGAC	40	6.7739165E-9	45.0	5
CTGTACA	20	7.021517E-4	45.0	16
GTTTCGG	25	3.881053E-5	45.0	2
TTTACGG	25	3.881053E-5	45.0	2
AGATAGT	20	7.021517E-4	45.0	27
TCTCTCG	50	2.1827873E-11	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content