SRR2933782.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933782.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	396533
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC	16404	4.136856201123236	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG	13417	3.3835771549908835	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	11573	2.9185465018043897	No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	9033	2.27799451748026	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	6021	1.5184108258329065	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGACCTTAT	5676	1.4314067177258891	No Hit
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	4650	1.1726640657902367	TruSeq Adapter, Index 20 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT	3663	0.9237566608579866	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGACCTTATCGTA	3594	0.906355839236583	No Hit
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	3372	0.8503705870633718	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTC	2934	0.7399131976405494	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCT	2903	0.7320954372019479	No Hit
TGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCTT	1939	0.48898830614349875	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGACCTTATCGT	1198	0.302118613078861	No Hit
CGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	1197	0.30186642725826096	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATG	1059	0.26706478401545397	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTAT	854	0.2153666907924435	No Hit
TCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	833	0.21007078855984243	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	810	0.20427051468604124	No Hit
TTCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT	764	0.1926699669384389	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGACCTTA	720	0.18157379083203667	No Hit
TGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	651	0.16417296921063315	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT	566	0.14273717445962883	No Hit
TTTTTTGGGATTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGGAA	547	0.13794564386822786	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	529	0.13340629909742693	No Hit
TTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	500	0.12609291030002545	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCC	481	0.12130137970862451	No Hit
TGCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT	419	0.10566585883142134	TruSeq Adapter, Index 20 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT	399	0.10062214241942033	TruSeq Adapter, Index 20 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	40	6.7957444E-9	45.000004	1
CGGGTCA	40	6.7957444E-9	45.000004	6
CCGGGTA	20	7.02769E-4	45.000004	5
TCTTGCG	60	0.0	45.000004	1
GGTACAA	30	2.1618907E-6	45.000004	8
ACTTATG	20	7.02769E-4	45.000004	17
TACGGGA	40	6.7957444E-9	45.000004	4
TCGGCGT	20	7.02769E-4	45.000004	4
TCGTGCG	20	7.02769E-4	45.000004	1
GGACGGA	20	7.02769E-4	45.000004	8
CAAGATA	30	2.1618907E-6	45.000004	12
AACTCGG	30	2.1618907E-6	45.000004	10
GTCCAGG	20	7.02769E-4	45.000004	3
CGTCCGG	20	7.02769E-4	45.000004	2
TAGCTAC	20	7.02769E-4	45.000004	23
GTCGGCG	20	7.02769E-4	45.000004	3
CGGGTTG	50	2.1827873E-11	45.0	6
TTGTGCG	35	1.2092278E-7	45.0	1
CAACCTA	90	0.0	45.0	21
ATAGCGC	25	3.886169E-5	45.0	27

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content