SRR2933781.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933781.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	244121
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC	11704	4.794343788531098	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG	10179	4.169653573432846	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	8947	3.664985806219047	No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	6974	2.856780039406688	RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGACTATGT	4069	1.6667963837605122	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	3470	1.4214262599284782	RNA PCR Primer, Index 38 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	2705	1.1080570700595196	RNA PCR Primer, Index 27 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	2683	1.0990451456449875	RNA PCR Primer, Index 38 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	2675	1.0957680822215214	RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTA	2620	1.0732382711851909	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT	2383	0.9761552672650038	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC	2174	0.8905419853269486	No Hit
TGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCTT	1465	0.6001122394222537	RNA PCR Primer, Index 27 (96% over 25bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGACTATGTCGT	885	0.3625251412209519	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATG	745	0.3051765313102928	No Hit
TCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	690	0.2826467202739625	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTAT	688	0.2818274544180959	No Hit
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	681	0.27896002392256297	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCGACTATG	584	0.23922562991303492	No Hit
TGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	506	0.2072742615342392	RNA PCR Primer, Index 27 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	493	0.20194903347110654	RNA PCR Primer, Index 27 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	440	0.18023848829064276	No Hit
TTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	391	0.16016647482191207	RNA PCR Primer, Index 27 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT	372	0.1523834491911798	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	358	0.1466485882001139	No Hit
TGCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	353	0.14460042356044747	RNA PCR Primer, Index 38 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCC	304	0.12452841009171682	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCGACTATGTCG	264	0.10814309297438565	No Hit
CGTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	258	0.10568529540678596	RNA PCR Primer, Index 38 (95% over 21bp)
GCCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	253	0.1036371307671196	RNA PCR Primer, Index 38 (95% over 21bp)
TTTCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	248	0.10158896612745319	No Hit
ACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	246	0.10076970027158663	RNA PCR Primer, Index 27 (95% over 23bp)
TTGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	245	0.10036006734365334	RNA PCR Primer, Index 38 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAAGG	30	2.1584547E-6	45.000004	36
TAGAATA	30	2.1584547E-6	45.000004	10
TACGGGA	30	2.1584547E-6	45.000004	4
AATATTC	30	2.1584547E-6	45.000004	34
ATTGGGA	30	2.1584547E-6	45.000004	4
GCGTCTT	30	2.1584547E-6	45.000004	9
CGGACAG	25	3.8817518E-5	45.0	6
TTGGCAC	20	7.022361E-4	45.0	5
GTACCCA	20	7.022361E-4	45.0	25
TCGCAGG	25	3.8817518E-5	45.0	2
ACAAACC	25	3.8817518E-5	45.0	35
ACCGGGA	25	3.8817518E-5	45.0	4
GTTCTCG	20	7.022361E-4	45.0	1
CTCGTAC	20	7.022361E-4	45.0	29
TTTACAT	20	7.022361E-4	45.0	31
TCCTGAT	20	7.022361E-4	45.0	14
CTTTTCG	40	6.7775545E-9	45.0	1
CTTCAGC	25	3.8817518E-5	45.0	13
TTCAATC	25	3.8817518E-5	45.0	45
TACGGTT	20	7.022361E-4	45.0	33

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content