##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933781.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	244121
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.66534218686635	30.0	16.0	31.0	16.0	34.0
2	26.664277141253724	31.0	16.0	33.0	16.0	34.0
3	27.602971477259228	31.0	25.0	33.0	16.0	34.0
4	31.70954157979035	35.0	28.0	35.0	19.0	37.0
5	33.55587188320546	35.0	33.0	37.0	28.0	37.0
6	32.25378398417178	35.0	32.0	37.0	17.0	37.0
7	33.47533804957378	35.0	32.0	35.0	29.0	37.0
8	32.821268960884154	35.0	32.0	37.0	26.0	37.0
9	34.417280774697794	37.0	32.0	39.0	28.0	39.0
10	33.97815017962404	35.0	32.0	39.0	27.0	39.0
11	34.578848194133236	37.0	33.0	39.0	27.0	39.0
12	34.60100523920515	37.0	34.0	39.0	27.0	39.0
13	34.87529954407855	37.0	34.0	39.0	30.0	39.0
14	34.55498297975184	37.0	33.0	39.0	27.0	40.0
15	35.0681629192081	37.0	33.0	39.0	27.0	40.0
16	34.15660676467817	36.0	32.0	38.0	27.0	40.0
17	34.266171283912485	36.0	32.0	38.0	27.0	40.0
18	34.29773759733902	36.0	32.0	39.0	27.0	40.0
19	33.71684943122468	36.0	32.0	39.0	25.0	40.0
20	34.668500456740716	36.0	32.0	39.0	27.0	40.0
21	34.340712187808506	37.0	32.0	39.0	27.0	40.0
22	35.544619266675134	37.0	34.0	39.0	30.0	40.0
23	36.11098594549424	38.0	34.0	39.0	30.0	40.0
24	36.26247639490253	38.0	35.0	40.0	31.0	40.0
25	34.37763649993241	37.0	32.0	39.0	25.0	40.0
26	35.19006558223176	37.0	34.0	39.0	28.0	40.0
27	35.530986682833515	37.0	34.0	39.0	30.0	40.0
28	35.46515867131463	38.0	34.0	39.0	29.0	40.0
29	36.10776623068069	38.0	35.0	40.0	30.0	40.0
30	34.12027642029977	37.0	32.0	39.0	24.0	40.0
31	34.690342084458116	37.0	33.0	39.0	25.0	40.0
32	35.401665567484976	37.0	34.0	39.0	30.0	40.0
33	35.927937375317974	38.0	35.0	40.0	30.0	40.0
34	36.15106852749251	38.0	35.0	40.0	30.0	40.0
35	36.50373790046739	38.0	35.0	40.0	31.0	41.0
36	36.268784741992704	38.0	35.0	40.0	30.0	41.0
37	36.549321852687804	38.0	35.0	40.0	31.0	41.0
38	35.91264168178895	38.0	35.0	40.0	30.0	41.0
39	36.04874222209478	38.0	35.0	40.0	30.0	41.0
40	35.73845347184388	38.0	34.0	40.0	30.0	41.0
41	34.986633677561535	37.0	33.0	40.0	27.0	40.0
42	36.06833086870855	38.0	35.0	40.0	30.0	41.0
43	36.09703384796884	38.0	35.0	40.0	31.0	40.0
44	36.4968192003146	38.0	35.0	40.0	31.0	41.0
45	36.81090934413672	39.0	35.0	40.0	32.0	41.0
46	36.612339782321065	39.0	35.0	40.0	31.0	41.0
47	36.21743315814698	38.0	35.0	40.0	30.0	41.0
48	36.44242814014362	38.0	35.0	40.0	31.0	41.0
49	36.7308179140672	39.0	35.0	40.0	32.0	41.0
50	36.47382240774042	38.0	35.0	40.0	31.0	41.0
51	35.90748440322627	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	4.0
13	4.0
14	8.0
15	13.0
16	21.0
17	54.0
18	105.0
19	196.0
20	326.0
21	462.0
22	552.0
23	790.0
24	1064.0
25	1576.0
26	2271.0
27	2997.0
28	4164.0
29	5825.0
30	8542.0
31	11875.0
32	16348.0
33	21619.0
34	28239.0
35	36870.0
36	41618.0
37	38224.0
38	18810.0
39	1537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.83384878810099	3.356122578557355	40.9424015140033	14.867627119338362
2	15.3296930620471	25.59017864091987	37.66902478688847	21.41110351014456
3	15.045407810061404	25.35873603663757	42.4945006779425	17.10135547535853
4	17.494193453246545	3.0157176154448	58.09004551021829	21.400043421090363
5	30.42097976003703	3.6346729695519846	41.71374031730167	24.23060695310932
6	15.74588011682731	20.92282106004809	43.759037526472525	19.57226129665207
7	58.74095223270427	1.2780547351518303	32.754248917544984	7.226744114598908
8	53.72950299236854	16.74128813170518	15.799132397458637	13.730076478467645
9	49.51765722735856	4.710369038304775	18.46911982172775	27.30285391260891
10	33.076220398900546	20.322299187697904	26.626140315663132	19.975340097738417
11	27.397069486033566	22.80877106025291	29.087624579614207	20.70653487409932
12	20.81508760000164	15.303066921731437	36.607665870613346	27.274179607653583
13	20.271095071706245	23.396594311837163	41.07471294972575	15.257597666730843
14	17.835417682214967	27.8923156959049	27.511766705854885	26.76049991602525
15	10.83847764018663	24.814743508342175	43.39077752426051	20.956001327210686
16	10.589830452931128	24.217908332343388	28.155300035638064	37.03696117908742
17	12.091544766734529	27.355696560312303	33.729584918954124	26.82317375399904
18	11.951450305381348	23.00908156201228	36.8804814006169	28.158986731989465
19	13.741136567521844	24.745515543521453	30.241970170530188	31.271377718426518
20	15.407523318354421	25.668008897227196	41.88128018482637	17.043187599592006
21	20.653282593467992	30.679458137562932	28.534210493976346	20.13304877499273
22	13.892700750857156	23.85579282405037	31.81905694307331	30.432449482019162
23	15.469377890472346	29.554196484530216	28.59688433195014	26.3795412930473
24	22.529811036330344	20.77781100355971	25.1424498506888	31.54992810942115
25	13.41343022517522	32.36878433235977	22.854649948181436	31.363135494283572
26	15.942503922235286	21.22799759135838	28.617775611274737	34.21172287513159
27	24.245353738514915	25.86340380385137	23.198331974717455	26.69291048291626
28	11.804801717181233	27.38314196648383	32.13365503172607	28.678401284608864
29	19.20523019322385	18.262664826049377	24.57346971378947	37.95863526693729
30	20.413237697699092	27.320468128510043	30.522568726164483	21.74372544762638
31	18.71653811019945	31.36272586135564	21.115758169104666	28.804977859340248
32	20.107241900532934	27.041508104587482	26.388143584533903	26.463106410345688
33	21.06045772383367	21.33245398798137	25.906824894212292	31.700263393972662
34	19.440769126785487	25.817934548850772	27.43762314589896	27.303673178464777
35	20.927736655183292	17.285280660000574	29.10564842844327	32.681334256372864
36	25.479577750377885	23.75952908598605	24.215040901847853	26.545852261788212
37	21.063325154329206	21.955505671367888	33.10038874164861	23.880780432654298
38	18.896776598490092	28.492427935327154	28.08238537446594	24.52841009171681
39	21.00884397491408	24.640239881042596	29.191261710381326	25.15965443366199
40	19.76355987399691	19.73734336660918	38.687781878658534	21.81131488073537
41	22.392174372544762	28.02995235969048	23.40888329967516	26.168989968089594
42	21.02277149446381	16.442256094313883	36.626918618226206	25.908053792996093
43	31.449568042077498	16.821985818508033	23.598543345308272	28.129902794106204
44	21.948951544520956	18.00705387901901	31.418026306626633	28.6259682698334
45	18.18893089902139	16.623313848460395	28.906976458395633	36.28077879412259
46	27.92959229234683	20.287480388823575	29.439499264708896	22.343428054120704
47	13.69566731252125	16.8277206794991	46.458108888624906	23.018503119354747
48	19.78322225453771	21.95796346893549	28.010699612077616	30.24811466444919
49	18.46993908758362	13.680101261259786	43.64147287615568	24.208486775000924
50	26.068629900745943	13.365093539679092	31.383207507752303	29.183069051822663
51	18.897186231418026	14.0569635549584	27.739112980857854	39.30673723276572
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	88.0
1	125.0
2	162.0
3	453.0
4	744.0
5	536.5
6	329.0
7	340.5
8	352.0
9	455.5
10	559.0
11	539.0
12	519.0
13	535.0
14	551.0
15	494.0
16	437.0
17	476.0
18	515.0
19	490.0
20	465.0
21	532.0
22	599.0
23	637.5
24	676.0
25	737.0
26	968.5
27	1139.0
28	1177.5
29	1216.0
30	1541.0
31	1866.0
32	1945.0
33	2024.0
34	2427.0
35	2830.0
36	3297.5
37	3765.0
38	4244.5
39	4724.0
40	5662.5
41	6601.0
42	8152.0
43	9703.0
44	12138.5
45	14574.0
46	40187.0
47	65800.0
48	54193.0
49	42586.0
50	39530.0
51	36474.0
52	26143.5
53	15813.0
54	12550.0
55	9287.0
56	7619.5
57	5952.0
58	5133.5
59	4315.0
60	3618.5
61	2922.0
62	2612.0
63	2302.0
64	1875.0
65	1448.0
66	1132.5
67	817.0
68	602.0
69	387.0
70	335.0
71	283.0
72	230.5
73	178.0
74	162.0
75	123.0
76	100.0
77	65.0
78	30.0
79	29.0
80	28.0
81	18.0
82	8.0
83	5.5
84	3.0
85	1.5
86	0.0
87	2.5
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	244121.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.992417694503953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.26581107336867	22.08699784123447
2	12.561625186692924	7.786302694155767
3	4.906223978641008	4.5616722854649945
4	2.5310934588086016	3.137788227968917
5	1.6746190142613568	2.5950245984573224
6	1.204086757689105	2.239053584083303
7	0.8564744445472448	1.8580949611053534
8	0.7295893416513567	1.80893900975336
9	0.6317820748357763	1.7622408559689662
>10	3.382281024068518	16.714661991389516
>50	0.14274574075787413	3.048078616751529
>100	0.08723350824092309	5.026605658669267
>500	0.009252038752825176	1.9576357625931402
>1k	0.011895478396489512	9.931140704814416
>5k	0.0026434396436643363	6.521765845625735
>10k+	0.0026434396436643363	8.963997361963944
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC	11704	4.794343788531098	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG	10179	4.169653573432846	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	8947	3.664985806219047	No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	6974	2.856780039406688	RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGACTATGT	4069	1.6667963837605122	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	3470	1.4214262599284782	RNA PCR Primer, Index 38 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	2705	1.1080570700595196	RNA PCR Primer, Index 27 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	2683	1.0990451456449875	RNA PCR Primer, Index 38 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	2675	1.0957680822215214	RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTA	2620	1.0732382711851909	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT	2383	0.9761552672650038	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC	2174	0.8905419853269486	No Hit
TGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCTT	1465	0.6001122394222537	RNA PCR Primer, Index 27 (96% over 25bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGACTATGTCGT	885	0.3625251412209519	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATG	745	0.3051765313102928	No Hit
TCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	690	0.2826467202739625	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTAT	688	0.2818274544180959	No Hit
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	681	0.27896002392256297	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCGACTATG	584	0.23922562991303492	No Hit
TGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	506	0.2072742615342392	RNA PCR Primer, Index 27 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	493	0.20194903347110654	RNA PCR Primer, Index 27 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	440	0.18023848829064276	No Hit
TTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	391	0.16016647482191207	RNA PCR Primer, Index 27 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT	372	0.1523834491911798	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	358	0.1466485882001139	No Hit
TGCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	353	0.14460042356044747	RNA PCR Primer, Index 38 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCC	304	0.12452841009171682	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCGACTATGTCG	264	0.10814309297438565	No Hit
CGTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	258	0.10568529540678596	RNA PCR Primer, Index 38 (95% over 21bp)
GCCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	253	0.1036371307671196	RNA PCR Primer, Index 38 (95% over 21bp)
TTTCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	248	0.10158896612745319	No Hit
ACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	246	0.10076970027158663	RNA PCR Primer, Index 27 (95% over 23bp)
TTGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	245	0.10036006734365334	RNA PCR Primer, Index 38 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2923918876294953	0.0
2	0.0	0.0	0.0	7.129661110678721	0.0
3	0.0	0.0	0.0	8.62359239885139	0.0
4	0.0	0.0	0.0	11.796609058622568	0.0
5	0.0	0.0	0.0	18.399891856907026	0.0
6	0.0	0.0	0.0	19.94297909643169	0.0
7	0.0	0.0	0.0	22.25453770875918	0.0
8	0.0	0.0	0.0	25.130570495778734	0.0
9	0.0	0.0	0.0	25.925668008897226	0.0
10	0.0	0.0	0.0	31.95751287271476	0.0
11	0.0	0.0	0.0	34.98306167842996	0.0
12	0.0	0.0	0.0	42.550210756141425	0.0
13	0.0	0.0	0.0	43.89872235489778	0.0
14	0.0	0.0	0.0	44.490232302833434	0.0
15	0.0	0.0	0.0	46.15416125609841	0.0
16	0.0	0.0	0.0	47.57067192089169	0.0
17	0.0	0.0	0.0	49.27474490109413	0.0
18	0.0	0.0	0.0	50.99438393255804	0.0
19	0.0	0.0	0.0	53.85280250367646	0.0
20	0.0	0.0	0.0	55.16772420234228	0.0
21	0.0	0.0	0.0	56.39580372028625	0.0
22	0.0	0.0	0.0	57.67959331642915	0.0
23	0.0	0.0	0.0	58.83393890734513	0.0
24	0.0	0.0	0.0	59.832623985646464	0.0
25	0.0	0.0	0.0	60.61338434628729	0.0
26	0.0	0.0	0.0	61.338434628729196	0.0
27	0.0	0.0	0.0	62.01187116225151	0.0
28	0.0	0.0	0.0	62.65745265667435	0.0
29	0.0	0.0	0.0	63.35997312807993	0.0
30	0.0	0.0	0.0	64.10796285448609	0.0
31	0.0	0.0	0.0	64.84325396012633	0.0
32	0.0	0.0	0.0	65.46139004837765	0.0
33	0.0	0.0	0.0	66.0553577938809	0.0
34	0.0	0.0	0.0	66.63990398204169	0.0
35	0.0	0.0	0.0	67.24902814587848	0.0
36	0.0	0.0	0.0	67.82128534620126	0.0
37	0.0	0.0	0.0	68.39681960994753	0.0
38	0.0	0.0	0.0	68.91418599792726	0.0
39	0.0	0.0	0.0	69.45776889329471	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAAGG	30	2.1584547E-6	45.000004	36
TAGAATA	30	2.1584547E-6	45.000004	10
TACGGGA	30	2.1584547E-6	45.000004	4
AATATTC	30	2.1584547E-6	45.000004	34
ATTGGGA	30	2.1584547E-6	45.000004	4
GCGTCTT	30	2.1584547E-6	45.000004	9
CGGACAG	25	3.8817518E-5	45.0	6
TTGGCAC	20	7.022361E-4	45.0	5
GTACCCA	20	7.022361E-4	45.0	25
TCGCAGG	25	3.8817518E-5	45.0	2
ACAAACC	25	3.8817518E-5	45.0	35
ACCGGGA	25	3.8817518E-5	45.0	4
GTTCTCG	20	7.022361E-4	45.0	1
CTCGTAC	20	7.022361E-4	45.0	29
TTTACAT	20	7.022361E-4	45.0	31
TCCTGAT	20	7.022361E-4	45.0	14
CTTTTCG	40	6.7775545E-9	45.0	1
CTTCAGC	25	3.8817518E-5	45.0	13
TTCAATC	25	3.8817518E-5	45.0	45
TACGGTT	20	7.022361E-4	45.0	33
>>END_MODULE