SRR2933780.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933780.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	578472
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGC	14282	2.4689181153106805	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCG	11085	1.9162552379371862	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC	9106	1.5741470632977914	No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	6602	1.141282551273009	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTTCCGT	4475	0.7735897329516381	No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	4142	0.7160242846671921	No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	3473	0.6003747804560982	No Hit
TCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	2991	0.5170518192756088	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTA	2696	0.46605540112572436	No Hit
GAACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT	2598	0.4491142181471186	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTC	2079	0.35939509604613534	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCT	2060	0.3561105809788546	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1239	0.214184956229515	No Hit
TGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTT	1041	0.17995685184416876	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGT	961	0.16612731471877637	No Hit
CGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	939	0.16232419200929343	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATG	839	0.14503727060255295	No Hit
TCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	728	0.12584878784107095	No Hit
TTCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT	705	0.12187279591752065	No Hit
TGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	672	0.11616811185329627	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTAT	664	0.11478515814075704	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTG	30	2.1636242E-6	45.000004	17
TCGATGG	65	0.0	45.000004	2
CGTATGG	35	1.2105374E-7	45.0	2
GTGGCGT	20	7.0303783E-4	45.0	34
TGCGCCG	20	7.0303783E-4	45.0	1
TCTACCG	20	7.0303783E-4	45.0	1
TACGGCT	1685	0.0	42.59644	7
TGATACG	3205	0.0	41.911076	4
GATACGG	3220	0.0	41.85559	5
TTTCGCG	140	0.0	41.785713	1
ATACGGC	3210	0.0	41.775703	6
ATGATAC	4495	0.0	41.59622	3
GGTGCGC	65	0.0	41.538464	8
CGCCGGT	65	0.0	41.538464	28
ACGGCTG	1735	0.0	41.368877	8
CGACCAC	970	0.0	41.28866	12
TCGTTTG	240	0.0	41.250004	1
ACACGAC	60	3.6379788E-12	41.250004	26
CACGACC	60	3.6379788E-12	41.250004	27
TCAAGCG	60	3.6379788E-12	41.250004	17

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content