##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933780.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	578472
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.13559342543805	31.0	16.0	33.0	16.0	34.0
2	28.41942047324676	31.0	26.0	33.0	16.0	34.0
3	29.330591627598224	31.0	28.0	34.0	16.0	34.0
4	32.72673871855509	35.0	32.0	37.0	28.0	37.0
5	33.8520239527583	35.0	33.0	37.0	28.0	37.0
6	33.1605903137922	35.0	33.0	37.0	28.0	37.0
7	33.541353773389204	35.0	32.0	35.0	29.0	37.0
8	33.697229943713786	35.0	35.0	37.0	28.0	37.0
9	34.788838526324525	37.0	33.0	39.0	28.0	39.0
10	33.571738995145836	35.0	31.0	39.0	27.0	39.0
11	33.9451693426821	35.0	32.0	39.0	27.0	39.0
12	34.20812589027645	37.0	32.0	39.0	27.0	39.0
13	34.55776597657277	37.0	33.0	39.0	27.0	39.0
14	34.3112060739327	37.0	32.0	39.0	25.0	40.0
15	34.83372920383355	37.0	32.0	39.0	27.0	40.0
16	34.55976780207166	36.0	32.0	39.0	27.0	40.0
17	34.41882926053465	36.0	32.0	39.0	27.0	40.0
18	34.30267670691062	36.0	32.0	39.0	27.0	40.0
19	34.10991888976476	36.0	32.0	39.0	25.0	40.0
20	35.13901623587658	37.0	33.0	39.0	27.0	40.0
21	34.4425002420169	37.0	32.0	39.0	25.0	40.0
22	35.39566478584962	37.0	34.0	39.0	28.0	40.0
23	36.191691214095066	38.0	34.0	39.0	30.0	40.0
24	36.31429870417237	38.0	35.0	40.0	31.0	40.0
25	34.23802880692583	37.0	32.0	39.0	24.0	40.0
26	34.9717877442642	37.0	33.0	39.0	27.0	40.0
27	35.565252942234025	37.0	34.0	39.0	30.0	40.0
28	35.76223049689527	38.0	34.0	40.0	30.0	40.0
29	36.26646579263992	38.0	35.0	40.0	30.0	41.0
30	34.1808505856809	37.0	33.0	39.0	24.0	40.0
31	34.46005338201331	37.0	32.0	39.0	25.0	40.0
32	35.23511768936094	37.0	34.0	39.0	29.0	40.0
33	36.06606715623228	38.0	35.0	40.0	30.0	40.0
34	36.34857348324552	38.0	35.0	40.0	30.0	41.0
35	36.537535092450454	38.0	35.0	40.0	31.0	41.0
36	36.46224017757125	38.0	35.0	40.0	31.0	41.0
37	36.65988673609094	39.0	35.0	40.0	31.0	41.0
38	36.20501078703896	38.0	35.0	40.0	30.0	41.0
39	36.135913233484075	38.0	35.0	40.0	30.0	41.0
40	35.62660249761441	38.0	34.0	40.0	30.0	41.0
41	35.10708902073048	37.0	33.0	40.0	28.0	41.0
42	36.07616098964168	38.0	35.0	40.0	30.0	41.0
43	36.17933452267353	38.0	35.0	40.0	31.0	41.0
44	36.62264206392012	38.0	35.0	40.0	31.0	41.0
45	36.91080121423336	39.0	35.0	40.0	32.0	41.0
46	36.7169214758882	39.0	35.0	40.0	32.0	41.0
47	36.57203114411761	39.0	35.0	40.0	31.0	41.0
48	36.61315845883638	38.0	35.0	40.0	32.0	41.0
49	36.813491404942674	39.0	35.0	40.0	32.0	41.0
50	36.644686346098	39.0	35.0	40.0	32.0	41.0
51	36.01173609094304	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	11.0
11	13.0
12	16.0
13	19.0
14	26.0
15	39.0
16	86.0
17	144.0
18	301.0
19	564.0
20	810.0
21	1117.0
22	1460.0
23	1948.0
24	2624.0
25	3776.0
26	5100.0
27	7124.0
28	9523.0
29	13160.0
30	18412.0
31	25937.0
32	36262.0
33	47686.0
34	60129.0
35	78742.0
36	96479.0
37	100932.0
38	59678.0
39	6345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.878417624362115	3.4938596855163255	48.995284127840236	16.632438562281322
2	17.697485790150605	13.8110401194872	47.901886348863904	20.589587741498292
3	18.61196393256718	13.692970446279162	48.721113554329335	18.973952066824324
4	17.93950268984497	3.8022583634125766	54.746297141434674	23.511941805307774
5	24.21793967555906	5.06783387960005	46.95698322477146	23.757243220069423
6	20.069251407155402	12.980230676679252	48.56656847695307	18.38394943921227
7	74.66083060199975	1.567059425521028	19.011118947848814	4.760991024630406
8	73.19904852784578	9.259393713092424	10.03108188468932	7.510475874372485
9	69.02149110069286	3.9756461851221845	11.762367063574382	15.240495650610574
10	37.69966394224785	25.890967929303404	19.87079754940602	16.53857057904272
11	30.409769185025375	23.736671783595405	26.133676305854042	19.719882725525174
12	26.402487933728857	18.628559377117647	30.97176699995851	23.997185689194982
13	24.93033370673084	20.72235129790206	34.65491847487864	19.692396520488458
14	20.0448768479719	25.890276452447136	28.23991481005131	25.82493188952966
15	15.692202906968703	25.096115283021476	37.678228159703494	21.533453650306324
16	18.45845607047532	24.058208521760776	30.203536212642963	27.27979919512094
17	17.752458200224037	24.567308357189287	33.00315313446459	24.677080308122086
18	17.826791962273024	23.996839950766848	32.47832220055595	25.69804588640418
19	19.97676637762934	26.047933175676608	29.289230939440458	24.686069507253592
20	23.112441051598005	25.428542781673098	34.0574824710617	17.401533695667208
21	24.743634955538038	27.045734279273674	27.970065966892086	20.2405647982962
22	19.629126388139788	23.81169702250066	30.038791851636727	26.52038473772283
23	20.297611638938445	27.759511264157986	28.827151530238282	23.115725566665287
24	23.699159164142777	22.04410930866144	27.598051418219033	26.65868010897675
25	19.28598099821599	27.895040727986835	24.50957003969077	28.309408234106403
26	19.328852563304707	24.195639546944363	27.844908655907286	28.630599233843647
27	20.59771259455946	28.777019458158737	26.4908241021173	24.1344438451645
28	16.01408538356221	25.277109350150052	31.556410681934477	27.152394584353264
29	19.65177225518262	22.78087789901672	29.535742438700574	28.031607407100083
30	19.81962826204207	26.099966809110896	30.38695044876848	23.69345448007855
31	20.05680482374255	23.76882545741194	25.243745591835044	30.93062412701047
32	20.417410004287156	27.4110069285981	27.37729743185496	24.79428563525979
33	21.659302438147396	22.503422810438533	26.688759352224483	29.14851539918959
34	20.89936937310708	22.21196531552089	26.640010233857474	30.24865507751456
35	18.467618138820892	23.155312616686718	26.58884094649352	31.788228297998867
36	20.728747458822554	24.247500311164586	24.721507696137408	30.30224453387545
37	17.606902321979284	26.43930907632522	31.036765824447855	24.917022777247645
38	19.741318508069536	23.65092865341797	27.74222434275125	28.865528495761243
39	21.343470384046245	20.677405302244534	32.22887192465668	25.750252389052537
40	20.01825498900552	20.646634582140535	32.14122723312451	27.19388319572944
41	19.861808350274515	23.178304222157685	25.78206032444094	31.17782710312686
42	20.633150783443277	21.616603742272748	28.290046882130852	29.46019859215312
43	25.56251642257534	20.89936937310708	25.761143149538785	27.776971054778798
44	19.87615649504211	21.74037809954501	30.10430928376827	28.27915612164461
45	19.247776901907095	18.74853061168043	28.22971552642133	33.773976959991145
46	22.843802292937255	22.10323057987249	28.997427706094676	26.055539421095574
47	15.749249747610946	21.666735814352293	36.79659516795973	25.787419270077034
48	19.254864539683858	21.9727143232516	28.787218741788713	29.985202395275827
49	18.847066063698847	18.658292881937243	35.55608568781203	26.93855536655188
50	22.066409437276135	18.40797825996764	30.025480922153534	29.50013138060269
51	18.121188233829812	18.384295177640407	27.711799361075386	35.7827172274544
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	100.0
1	321.5
2	543.0
3	1459.5
4	2376.0
5	1720.0
6	1064.0
7	1024.5
8	985.0
9	1032.5
10	1080.0
11	1070.0
12	1060.0
13	1124.5
14	1189.0
15	1085.0
16	981.0
17	1022.0
18	1063.0
19	1145.0
20	1227.0
21	1296.0
22	1365.0
23	1670.0
24	1975.0
25	2361.0
26	3402.5
27	4058.0
28	5001.5
29	5945.0
30	6743.0
31	7541.0
32	8408.5
33	9276.0
34	10447.5
35	11619.0
36	13122.5
37	14626.0
38	15902.0
39	17178.0
40	19358.5
41	21539.0
42	23233.5
43	24928.0
44	27092.0
45	29256.0
46	32598.5
47	35941.0
48	65321.5
49	94702.0
50	83735.5
51	72769.0
52	69155.5
53	65542.0
54	51920.0
55	38298.0
56	32914.0
57	27530.0
58	25645.0
59	23760.0
60	21641.0
61	19522.0
62	16428.0
63	13334.0
64	10948.0
65	8562.0
66	6938.0
67	5314.0
68	4409.5
69	3505.0
70	2878.0
71	2251.0
72	1960.0
73	1669.0
74	1319.0
75	753.5
76	538.0
77	421.0
78	304.0
79	232.0
80	160.0
81	108.5
82	57.0
83	34.0
84	11.0
85	11.5
86	12.0
87	6.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	578472.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.752704291657952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.2868186289468	22.317965675263434
2	12.358379200986036	7.848239205861714
3	4.734587330284913	4.510078543247014
4	2.4084681147378553	3.0590150337263218
5	1.431424803620507	2.2725804252553257
6	0.9585219926418074	1.826139923364363
7	0.7447929411207547	1.6554433012545076
8	0.5894806247274744	1.497408317010663
9	0.519148370082973	1.4835928210866762
>10	5.774832151720532	34.4664338622764
>50	0.11855372035050273	2.5559408875369765
>100	0.0623096297656117	3.4683266914110917
>500	0.004962713875137214	1.1557587202788941
>1k	0.005514126527930238	4.691319368452157
>5k	0.0011028253055860478	2.750289043802586
>10k+	0.0011028253055860478	4.441468180171899
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGC	14282	2.4689181153106805	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCG	11085	1.9162552379371862	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC	9106	1.5741470632977914	No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	6602	1.141282551273009	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTTCCGT	4475	0.7735897329516381	No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	4142	0.7160242846671921	No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	3473	0.6003747804560982	No Hit
TCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	2991	0.5170518192756088	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTA	2696	0.46605540112572436	No Hit
GAACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT	2598	0.4491142181471186	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTC	2079	0.35939509604613534	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCT	2060	0.3561105809788546	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1239	0.214184956229515	No Hit
TGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTT	1041	0.17995685184416876	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACTTCCGTCGT	961	0.16612731471877637	No Hit
CGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	939	0.16232419200929343	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATG	839	0.14503727060255295	No Hit
TCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	728	0.12584878784107095	No Hit
TTCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT	705	0.12187279591752065	No Hit
TGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	672	0.11616811185329627	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTAT	664	0.11478515814075704	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6709054197955995	0.0
2	0.0	0.0	0.0	3.2487311399687453	0.0
3	0.0	0.0	0.0	4.042373701752203	0.0
4	0.0	0.0	0.0	5.533370673083572	0.0
5	0.0	0.0	0.0	8.754269869587464	0.0
6	0.0	0.0	0.0	9.574188551909167	0.0
7	0.0	0.0	0.0	10.75920701434123	0.0
8	0.0	0.0	0.0	12.279764621278126	0.0
9	0.0	0.0	0.0	12.766737197306005	0.0
10	0.0	0.0	0.0	15.89169398000249	0.0
11	0.0	0.0	0.0	18.014527928750226	0.0
12	0.0	0.0	0.0	22.369276300322227	0.0
13	0.0	0.0	0.0	23.137507087637776	0.0
14	0.0	0.0	0.0	23.464057033011105	0.0
15	0.0	0.0	0.0	24.314573566222737	0.0
16	0.0	0.0	0.0	25.200009680675986	0.0
17	0.0	0.0	0.0	26.26834142361255	0.0
18	0.0	0.0	0.0	27.323189367851857	0.0
19	0.0	0.0	0.0	28.9644096862078	0.0
20	0.0	0.0	0.0	29.93265015419934	0.0
21	0.0	0.0	0.0	31.047483715720034	0.0
22	0.0	0.0	0.0	32.37287197997483	0.0
23	0.0	0.0	0.0	33.549592720131656	0.0
24	0.0	0.0	0.0	34.48343221452378	0.0
25	0.0	0.0	0.0	35.29747334356719	0.0
26	0.0	0.0	0.0	35.97909665463497	0.0
27	0.0	0.0	0.0	36.66763473426544	0.0
28	0.0	0.0	0.0	37.3738054737308	0.0
29	0.0	0.0	0.0	38.128379593135016	0.0
30	0.0	0.0	0.0	38.91441590949951	0.0
31	0.0	0.0	0.0	39.635107662946524	0.0
32	0.0	0.0	0.0	40.385878659641264	0.0
33	0.0	0.0	0.0	41.07026787813412	0.0
34	0.0	0.0	0.0	41.74774232806428	0.0
35	0.0	0.0	0.0	42.43991066119017	0.0
36	0.0	0.0	0.0	43.178926551328324	0.0
37	0.0	0.0	0.0	43.955455060919114	0.0
38	0.0	0.0	0.0	44.648487740115335	0.0
39	0.0	0.0	0.0	45.3721182702015	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTG	30	2.1636242E-6	45.000004	17
TCGATGG	65	0.0	45.000004	2
CGTATGG	35	1.2105374E-7	45.0	2
GTGGCGT	20	7.0303783E-4	45.0	34
TGCGCCG	20	7.0303783E-4	45.0	1
TCTACCG	20	7.0303783E-4	45.0	1
TACGGCT	1685	0.0	42.59644	7
TGATACG	3205	0.0	41.911076	4
GATACGG	3220	0.0	41.85559	5
TTTCGCG	140	0.0	41.785713	1
ATACGGC	3210	0.0	41.775703	6
ATGATAC	4495	0.0	41.59622	3
GGTGCGC	65	0.0	41.538464	8
CGCCGGT	65	0.0	41.538464	28
ACGGCTG	1735	0.0	41.368877	8
CGACCAC	970	0.0	41.28866	12
TCGTTTG	240	0.0	41.250004	1
ACACGAC	60	3.6379788E-12	41.250004	26
CACGACC	60	3.6379788E-12	41.250004	27
TCAAGCG	60	3.6379788E-12	41.250004	17
>>END_MODULE