SRR2933777.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933777.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	914166
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC	12707	1.390010129451325	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG	9939	1.0872204829319838	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	8466	0.9260900099106727	No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	6192	0.6773386890345954	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	5161	0.564558296851994	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	4302	0.47059286825368696	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAATGACT	3819	0.41775782516523263	No Hit
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	3315	0.36262560629032364	TruSeq Adapter, Index 15 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT	2540	0.2778488808378347	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTA	2434	0.2662536125823975	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTC	2016	0.22052887549963573	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCT	1868	0.20433925567128947	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1490	0.16299009151510777	No Hit
TGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTT	1275	0.13947138703473985	TruSeq Adapter, Index 15 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	960	0.10501375023792177	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATA	20	7.032527E-4	45.000004	12
CACTACG	20	7.032527E-4	45.000004	34
CGAATTA	20	7.032527E-4	45.000004	43
GCGATTA	20	7.032527E-4	45.000004	9
ACCGTTT	20	7.032527E-4	45.000004	15
CAGTCGA	20	7.032527E-4	45.000004	10
ATCGACG	20	7.032527E-4	45.000004	16
AGTCGAT	20	7.032527E-4	45.000004	11
ACACGAT	45	3.8380676E-10	45.0	15
GGTCGAC	25	3.8901766E-5	45.0	8
CTCGTAA	80	0.0	42.187504	33
TACGGCT	1400	0.0	41.946426	7
CGGTAGT	65	0.0	41.53846	12
CTTTGCG	285	0.0	41.05263	1
ACGGCTG	1435	0.0	40.923347	8
GTACACG	55	6.002665E-11	40.909092	13
CACGATC	50	1.0804797E-9	40.5	16
TACACGA	50	1.0804797E-9	40.5	14
TGGGACG	195	0.0	40.384617	6
AGCGGGA	190	0.0	40.263157	4