##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933777.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	914166
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.675801769044135	31.0	25.0	33.0	16.0	34.0
2	29.083096505448683	31.0	28.0	34.0	16.0	34.0
3	30.034714701706253	31.0	30.0	34.0	23.0	34.0
4	33.11042524005487	35.0	32.0	37.0	28.0	37.0
5	33.96943005974845	35.0	33.0	37.0	28.0	37.0
6	33.51383993716677	35.0	33.0	37.0	28.0	37.0
7	33.589589855671726	35.0	32.0	35.0	29.0	37.0
8	34.01377102189318	35.0	35.0	37.0	30.0	37.0
9	34.87600173272688	37.0	34.0	39.0	28.0	39.0
10	33.47000872926799	35.0	31.0	39.0	27.0	39.0
11	33.98023881877033	35.0	32.0	39.0	27.0	39.0
12	34.31885128083959	37.0	32.0	39.0	27.0	39.0
13	34.73421894929367	37.0	34.0	39.0	27.0	39.0
14	34.48579798417355	37.0	32.0	39.0	25.0	40.0
15	34.945457389576944	37.0	32.0	39.0	27.0	40.0
16	34.6698827127677	36.0	32.0	39.0	27.0	40.0
17	34.543029384159986	36.0	32.0	39.0	27.0	40.0
18	34.433730854133714	37.0	32.0	39.0	27.0	40.0
19	34.27919874508569	37.0	32.0	39.0	25.0	40.0
20	35.49188659390089	37.0	34.0	39.0	30.0	40.0
21	34.636293627196814	37.0	32.0	39.0	25.0	40.0
22	35.51193109347755	37.0	34.0	39.0	29.0	40.0
23	36.45541838134431	38.0	35.0	40.0	31.0	41.0
24	36.52914022179779	38.0	35.0	40.0	31.0	41.0
25	34.35830910359825	37.0	32.0	39.0	24.0	40.0
26	34.99436426207056	37.0	33.0	39.0	27.0	40.0
27	35.75714585753572	37.0	34.0	39.0	30.0	40.0
28	35.89676929572966	38.0	34.0	40.0	30.0	41.0
29	36.46258885147774	38.0	35.0	40.0	30.0	41.0
30	34.18119138099645	37.0	33.0	39.0	24.0	40.0
31	34.95084590763603	37.0	33.0	39.0	27.0	40.0
32	35.494694617826525	37.0	34.0	39.0	30.0	40.0
33	36.41696256478583	38.0	35.0	40.0	31.0	41.0
34	36.751043027196374	38.0	35.0	40.0	31.0	41.0
35	36.88121632176213	39.0	35.0	40.0	32.0	41.0
36	36.80408044053268	39.0	35.0	40.0	31.0	41.0
37	37.01395807763579	39.0	35.0	40.0	32.0	41.0
38	36.51391213412006	38.0	35.0	40.0	31.0	41.0
39	36.42225700802699	38.0	35.0	40.0	31.0	41.0
40	36.00272707582649	38.0	34.0	40.0	30.0	41.0
41	35.63524677137413	38.0	34.0	40.0	30.0	41.0
42	36.41814506336924	38.0	35.0	40.0	31.0	41.0
43	36.44696915002308	38.0	35.0	40.0	31.0	41.0
44	36.858076104339915	39.0	35.0	40.0	32.0	41.0
45	37.09144072301967	39.0	35.0	40.0	33.0	41.0
46	36.83198784465841	39.0	35.0	40.0	32.0	41.0
47	36.763911587173446	39.0	35.0	40.0	32.0	41.0
48	36.75990575015916	39.0	35.0	40.0	32.0	41.0
49	36.91278170485448	39.0	35.0	40.0	32.0	41.0
50	36.76421897117154	39.0	35.0	40.0	32.0	41.0
51	35.99699398139944	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	14.0
11	19.0
12	34.0
13	29.0
14	38.0
15	63.0
16	99.0
17	202.0
18	355.0
19	653.0
20	981.0
21	1376.0
22	2036.0
23	2820.0
24	3980.0
25	5386.0
26	7447.0
27	10231.0
28	13591.0
29	18551.0
30	26262.0
31	36825.0
32	52381.0
33	69887.0
34	89314.0
35	120721.0
36	158112.0
37	173120.0
38	107590.0
39	12038.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.3099196426032	3.68685774793637	51.36145951610539	17.64176309335504
2	19.474362424329936	9.380134461356034	51.061404602665164	20.084098511648868
3	20.18079867332629	8.945968237716126	49.94038281887534	20.93285027008224
4	19.31159111146116	4.204925582443451	52.442991754232814	24.040491551862573
5	22.025758997818777	5.41083348100892	47.13378095444372	25.42962656672858
6	21.458028410595013	9.965804897578776	50.36109415576602	18.21507253606019
7	79.03630194078536	2.202663411240409	14.185607427972599	4.575427220001619
8	78.72355786585807	6.435592660413973	8.608611565076803	6.232237908651165
9	73.63870456787936	4.815755562994029	10.446680362209927	11.098859506916686
10	39.23466853941188	24.952251560438697	18.72438922471411	17.088690675435313
11	28.296283169577517	25.562206426403954	25.793674234220042	20.347836169798484
12	25.382589157767843	21.183351820128948	30.916923184629486	22.51713583747372
13	24.028239947668148	21.942404333567424	33.84822887746864	20.181126841295782
14	21.19286869124426	24.803153912965477	29.94193614726428	24.06204124852598
15	18.030532747881676	24.84373735185951	35.53227750758615	21.593452392672667
16	20.163405770943132	24.426417083986934	30.2577431232402	25.152434021829734
17	19.930625291249076	24.802606966349654	31.01635807938602	24.25040966301525
18	19.830315281907225	23.892815965590493	31.752767002929446	24.524101749572836
19	21.677244614216672	25.62991841744278	29.364360520955714	23.32847644738483
20	23.521548602770174	25.363774194183552	32.258583233242106	18.85609396980417
21	24.63819481363341	25.751449955478545	29.092746831538253	20.51760839934979
22	21.293288089909275	24.165523548239598	29.485892058991475	25.055296302859652
23	21.428165125371105	25.83217927597395	28.368151954896593	24.371503643758356
24	22.98729114843475	23.02328023575587	27.977194513906667	26.01223410190272
25	20.41806411527009	26.201040073684652	26.51433109522778	26.866564715817475
26	19.83020589258406	25.722789952809443	28.5028102117121	25.9441939428944
27	21.01281386531549	26.039690822017008	29.617596804081536	23.329898508585966
28	17.816129674479253	26.745798903043866	31.158892367469367	24.279179055007518
29	20.388200830046184	23.927820549003133	29.326402425817633	26.35757619513305
30	20.736824602971453	24.9503919419449	31.093039994924336	23.219743460159314
31	22.23316115453867	27.74889899646235	26.334166879975847	23.68377296902313
32	20.767016056164856	30.657779878052782	26.03345563059663	22.54174843518573
33	20.434910071037425	25.30590724222953	27.311122925158017	26.94805976157503
34	22.83108319495584	25.800018814963583	27.016209309906515	24.35268868017406
35	19.492739830621574	25.07553332764509	27.920749623153785	27.51097721857956
36	19.667762747684776	26.15378388607758	27.596410279971035	26.58204308626661
37	19.337844549020637	28.025107037452717	30.76946637700374	21.867582036522908
38	19.389038752261627	30.11881868282128	27.322608804090287	23.16953376082681
39	22.216643366740833	25.50335497054145	29.26678524469298	23.013216418024733
40	21.399833290671495	23.99815788379791	31.599293782529653	23.002715043000943
41	21.016533102303082	25.905688901140493	27.08829687387192	25.9894811226845
42	19.213797056552092	25.71053834861502	29.09613790055635	25.979526694276533
43	20.451427858835267	23.349479197432412	28.239181942885644	27.95991100084667
44	20.240962801066765	23.1902083429049	29.9511248504101	26.617704005618236
45	20.97780928190285	22.755604561972333	27.884322978540006	28.382263177584814
46	22.539888816691935	24.87775743136367	28.817632683779532	23.764721068164864
47	17.516402929008517	24.494676021641585	33.981027515790345	24.007893533559553
48	19.04358726970813	24.91691880905656	29.69405994097352	26.345433980261788
49	19.626194804882264	23.468057223742733	32.75203847003717	24.15370950133783
50	21.071774710501156	22.072358849486854	29.340294869859523	27.51557157015247
51	19.065355745017865	22.40927796483352	27.812235414574594	30.71313087557402
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	165.0
1	604.0
2	1043.0
3	2038.0
4	3033.0
5	2214.0
6	1395.0
7	1409.0
8	1423.0
9	1475.0
10	1527.0
11	1555.0
12	1583.0
13	1604.0
14	1625.0
15	1564.0
16	1503.0
17	1542.5
18	1582.0
19	1809.5
20	2037.0
21	2186.0
22	2335.0
23	3005.0
24	3675.0
25	4216.5
26	5939.5
27	7121.0
28	8695.5
29	10270.0
30	12106.5
31	13943.0
32	15506.0
33	17069.0
34	19599.0
35	22129.0
36	24507.5
37	26886.0
38	28489.0
39	30092.0
40	35101.5
41	40111.0
42	47116.0
43	54121.0
44	84929.0
45	115737.0
46	111812.0
47	107887.0
48	104661.0
49	101435.0
50	86103.5
51	70772.0
52	63122.5
53	55473.0
54	49234.5
55	42996.0
56	39721.5
57	36447.0
58	35522.5
59	34598.0
60	32859.5
61	31121.0
62	26890.5
63	22660.0
64	19373.5
65	16087.0
66	13083.0
67	10079.0
68	8776.5
69	7474.0
70	6143.5
71	4813.0
72	3826.5
73	2840.0
74	2431.5
75	1616.0
76	1209.0
77	900.5
78	592.0
79	475.5
80	359.0
81	239.0
82	119.0
83	66.5
84	14.0
85	9.0
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	914166.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.35941082607571
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.22535085781196	21.320002770976217
2	12.114447722485854	7.355749906759307
3	4.85532690552868	4.422145926595323
4	2.4508906638014283	2.9763038620856395
5	1.4012532471704744	2.127061150111053
6	0.9054558929402976	1.6493464463199432
7	0.6569648710378495	1.3961546492696546
8	0.5475395126837703	1.3298381607260703
9	0.4438395249097128	1.2127235829825813
>10	6.169776693757834	41.6710225418593
>50	0.15934799921925594	3.099907726192238
>100	0.06066918343653616	3.312611279314907
>500	0.0040202470951920975	0.8568073789606263
>1k	0.0032892930778844432	2.5585497735629477
>5k	0.001461908034615308	3.301848482661269
>10k+	3.65477008653827E-4	1.4099263616229836
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC	12707	1.390010129451325	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG	9939	1.0872204829319838	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	8466	0.9260900099106727	No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	6192	0.6773386890345954	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	5161	0.564558296851994	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	4302	0.47059286825368696	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAATGACT	3819	0.41775782516523263	No Hit
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	3315	0.36262560629032364	TruSeq Adapter, Index 15 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT	2540	0.2778488808378347	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTA	2434	0.2662536125823975	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTC	2016	0.22052887549963573	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCT	1868	0.20433925567128947	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1490	0.16299009151510777	No Hit
TGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTT	1275	0.13947138703473985	TruSeq Adapter, Index 15 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG	960	0.10501375023792177	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.534038675689098	0.0
2	0.0	0.0	0.0	2.2803298306872057	0.0
3	0.0	0.0	0.0	2.8734387408851347	0.0
4	0.0	0.0	0.0	3.9687540337312917	0.0
5	0.0	0.0	0.0	6.130287059461848	0.0
6	0.0	0.0	0.0	6.8674617082674265	0.0
7	0.0	0.0	0.0	7.782175228568991	0.0
8	0.0	0.0	0.0	9.039824276991268	0.0
9	0.0	0.0	0.0	9.491711570983826	0.0
10	0.0	0.0	0.0	11.552168862110383	0.0
11	0.0	0.0	0.0	13.540538589271533	0.0
12	0.0	0.0	0.0	16.609018493358974	0.0
13	0.0	0.0	0.0	17.287451075625214	0.0
14	0.0	0.0	0.0	17.539484076196228	0.0
15	0.0	0.0	0.0	18.140687796308328	0.0
16	0.0	0.0	0.0	18.934635503836283	0.0
17	0.0	0.0	0.0	19.950205980095518	0.0
18	0.0	0.0	0.0	20.969495693342346	0.0
19	0.0	0.0	0.0	22.156698017646686	0.0
20	0.0	0.0	0.0	23.030937488377383	0.0
21	0.0	0.0	0.0	24.16388270839213	0.0
22	0.0	0.0	0.0	25.35666388817786	0.0
23	0.0	0.0	0.0	26.485014756619695	0.0
24	0.0	0.0	0.0	27.379491252135825	0.0
25	0.0	0.0	0.0	28.186456289120358	0.0
26	0.0	0.0	0.0	28.904706585018477	0.0
27	0.0	0.0	0.0	29.597031611326607	0.0
28	0.0	0.0	0.0	30.286950072525123	0.0
29	0.0	0.0	0.0	31.01296701036792	0.0
30	1.0938932316450185E-4	0.0	0.0	31.80024196918284	0.0
31	1.0938932316450185E-4	0.0	0.0	32.53971379377487	0.0
32	1.0938932316450185E-4	0.0	0.0	33.28049829024488	0.0
33	1.0938932316450185E-4	0.0	0.0	34.00093637260629	0.0
34	1.0938932316450185E-4	0.0	0.0	34.697746361164164	0.0
35	1.0938932316450185E-4	0.0	0.0	35.37169398118066	0.0
36	1.0938932316450185E-4	0.0	0.0	36.107555958108264	0.0
37	1.0938932316450185E-4	0.0	0.0	36.84976251577941	0.0
38	1.0938932316450185E-4	0.0	0.0	37.56987243017132	0.0
39	1.0938932316450185E-4	0.0	0.0	38.248961348376554	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATA	20	7.032527E-4	45.000004	12
CACTACG	20	7.032527E-4	45.000004	34
CGAATTA	20	7.032527E-4	45.000004	43
GCGATTA	20	7.032527E-4	45.000004	9
ACCGTTT	20	7.032527E-4	45.000004	15
CAGTCGA	20	7.032527E-4	45.000004	10
ATCGACG	20	7.032527E-4	45.000004	16
AGTCGAT	20	7.032527E-4	45.000004	11
ACACGAT	45	3.8380676E-10	45.0	15
GGTCGAC	25	3.8901766E-5	45.0	8
CTCGTAA	80	0.0	42.187504	33
TACGGCT	1400	0.0	41.946426	7
CGGTAGT	65	0.0	41.53846	12
CTTTGCG	285	0.0	41.05263	1
ACGGCTG	1435	0.0	40.923347	8
GTACACG	55	6.002665E-11	40.909092	13
CACGATC	50	1.0804797E-9	40.5	16
TACACGA	50	1.0804797E-9	40.5	14
TGGGACG	195	0.0	40.384617	6
AGCGGGA	190	0.0	40.263157	4
>>END_MODULE