SRR2933776.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933776.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	358556
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGC	9811	2.736253193364495	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG	8250	2.300895815437477	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	6715	1.8727897455348677	Illumina PCR Primer Index 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	4481	1.2497350483606466	Illumina PCR Primer Index 2 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTGTAACT	3280	0.9147803969254454	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2597	0.72429411305347	Illumina PCR Primer Index 2 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTA	2077	0.5792679525652896	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	2049	0.571458851615926	Illumina PCR Primer Index 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	1866	0.5204207989825858	Illumina PCR Primer Index 2 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	1820	0.5075915617086313	Illumina PCR Primer Index 2 (96% over 25bp)
GAATGATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTC	1644	0.4585057843126318	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCT	1529	0.4264326911277458	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGT	729	0.2033155211459298	No Hit
TGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTT	726	0.20247883175849796	Illumina PCR Primer Index 2 (96% over 26bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATG	605	0.16873235979874832	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	555	0.15478753667488482	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	553	0.1542297437499303	Illumina PCR Primer Index 2 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT	539	0.1503251932752485	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTAT	482	0.13442809491404412	No Hit
TGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	447	0.12466671872733966	Illumina PCR Primer Index 2 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	442	0.12327223641495332	Illumina PCR Primer Index 2 (95% over 23bp)
TTCCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	437	0.12187775410256697	Illumina PCR Primer Index 2 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCTGTAAC	422	0.11769430716540791	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGTT	25	3.885418E-5	45.000004	20
CCCTCGC	25	3.885418E-5	45.000004	27
CTTTTCG	60	0.0	45.000004	1
TTCGTCG	30	2.1613087E-6	45.000004	1
AACGGGG	50	2.1827873E-11	45.000004	4
AGGGCGA	30	2.1613087E-6	45.000004	6
GCGAGTT	25	3.885418E-5	45.000004	24
TCCGGCC	20	7.026788E-4	45.0	18
TCCGGAA	20	7.026788E-4	45.0	3
TTCTACG	20	7.026788E-4	45.0	1
CCGGGTT	20	7.026788E-4	45.0	5
TCCATCG	20	7.026788E-4	45.0	15
CGAAAGA	20	7.026788E-4	45.0	36
ACGCACG	20	7.026788E-4	45.0	14
CGCATGG	20	7.026788E-4	45.0	2
TCCTCGA	20	7.026788E-4	45.0	27
TCTACGG	20	7.026788E-4	45.0	2
CGCTATT	20	7.026788E-4	45.0	28
AGCGCTA	20	7.026788E-4	45.0	26
AACGCAC	20	7.026788E-4	45.0	13

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content