SRR2933775.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933775.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	510127
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGC	15104	2.9608313223961877	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCG	12673	2.4842833255248205	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC	9961	1.9526510065140639	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	7861	1.5409888125898061	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	7786	1.5262865913782253	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	5634	1.104430857413938	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTAGCGGTT	5011	0.9823044065497416	No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	4787	0.938393772531154	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTA	3310	0.6488580294710925	No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT	3170	0.6214138832094752	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT	2721	0.5333965855561458	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTC	2660	0.5214387789707269	No Hit
TGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCTT	1623	0.31815606701860516	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG	1541	0.30208163849394365	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1204	0.23601965784990794	No Hit
TTCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT	1155	0.22641420665834194	No Hit
TCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG	1129	0.22131743663832731	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGT	1112	0.21798493316370235	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATG	972	0.19054078690208517	No Hit
TTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG	834	0.16348869987277678	No Hit
TGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG	817	0.16015619639815185	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTAT	804	0.1576078113881445	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTTAGCGGT	653	0.12800733934882882	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCC	650	0.1274192505003656	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC	630	0.12349865817727743	No Hit
TGCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT	542	0.10624805195568947	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAT	20	7.029592E-4	45.000004	22
TCGTTGA	20	7.029592E-4	45.000004	24
ACCGGCG	30	2.1631186E-6	45.000004	20
CTCCGAC	20	7.029592E-4	45.000004	20
ACGTTGA	40	6.8030204E-9	45.000004	28
ACTTGCG	20	7.029592E-4	45.000004	1
ACAGGCG	20	7.029592E-4	45.000004	33
CTACGTA	80	0.0	45.000004	14
CCTCACG	40	6.8030204E-9	45.000004	24
GTCCGCG	40	6.8030204E-9	45.000004	1
TCTCCCG	20	7.029592E-4	45.000004	1
TTCGATA	20	7.029592E-4	45.000004	23
CGTCATA	20	7.029592E-4	45.000004	38
CGATAAG	20	7.029592E-4	45.000004	25
TACGTAT	80	0.0	45.000004	15
GTACGGC	20	7.029592E-4	45.000004	9
TTTAGCG	35	1.2101555E-7	45.0	1
TACTTGG	35	1.2101555E-7	45.0	2
TTCGTAC	55	1.8189894E-12	45.0	33
CGTTCAT	25	3.887745E-5	45.0	17

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content