##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933775.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	510127
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.14720451965883	31.0	16.0	33.0	16.0	34.0
2	28.22668864812096	31.0	25.0	33.0	16.0	34.0
3	29.044312494731702	31.0	28.0	34.0	16.0	34.0
4	32.58451522856073	35.0	32.0	37.0	27.0	37.0
5	33.847767320686806	35.0	33.0	37.0	28.0	37.0
6	33.0253191852225	35.0	33.0	37.0	27.0	37.0
7	33.69706563267579	35.0	32.0	36.0	30.0	37.0
8	33.57139692664768	35.0	35.0	37.0	28.0	37.0
9	34.84920813836555	37.0	33.0	39.0	29.0	39.0
10	34.05876967892309	35.0	32.0	39.0	27.0	39.0
11	34.6394172431571	37.0	33.0	39.0	27.0	39.0
12	34.559848821960024	37.0	33.0	39.0	27.0	39.0
13	34.99558737334036	37.0	34.0	39.0	30.0	39.0
14	34.60900912909923	37.0	33.0	39.0	27.0	40.0
15	35.068984782220895	37.0	33.0	39.0	27.0	40.0
16	34.38966375039941	36.0	32.0	39.0	27.0	40.0
17	34.40233902537995	36.0	32.0	38.0	27.0	40.0
18	34.43263148196429	37.0	32.0	39.0	27.0	40.0
19	34.11813724817546	36.0	32.0	39.0	26.0	40.0
20	35.247375653513735	37.0	33.0	39.0	29.0	40.0
21	34.54827131282994	37.0	32.0	39.0	26.0	40.0
22	35.604767048205645	37.0	34.0	39.0	30.0	40.0
23	36.39602295114746	38.0	35.0	39.0	31.0	40.0
24	36.53466881776499	38.0	35.0	40.0	31.0	40.0
25	34.46289061351389	37.0	32.0	39.0	25.0	40.0
26	34.994272014615966	37.0	33.0	39.0	27.0	40.0
27	35.891266292511474	37.0	34.0	39.0	30.0	40.0
28	35.94340624981622	38.0	34.0	40.0	30.0	40.0
29	36.60837987403137	38.0	35.0	40.0	31.0	41.0
30	34.5500473411523	37.0	33.0	39.0	25.0	40.0
31	35.2871481023353	37.0	34.0	39.0	27.0	40.0
32	35.58968452953872	37.0	34.0	39.0	30.0	40.0
33	36.29210569132785	38.0	35.0	40.0	30.0	40.0
34	36.60179131863242	38.0	35.0	40.0	31.0	41.0
35	36.84595600703354	39.0	35.0	40.0	31.0	41.0
36	36.58400947215105	38.0	35.0	40.0	31.0	41.0
37	36.941277368184785	39.0	35.0	40.0	31.0	41.0
38	36.45105826588281	38.0	35.0	40.0	30.0	41.0
39	36.37484979230662	38.0	35.0	40.0	31.0	41.0
40	36.052134076416266	38.0	35.0	40.0	30.0	41.0
41	35.453057767967586	37.0	34.0	40.0	30.0	41.0
42	36.42681920384532	38.0	35.0	40.0	31.0	41.0
43	36.300599654595814	38.0	35.0	40.0	31.0	41.0
44	36.87604263252092	39.0	35.0	40.0	32.0	41.0
45	37.162132174928985	39.0	36.0	40.0	33.0	41.0
46	36.89521824957315	39.0	35.0	40.0	32.0	41.0
47	36.72263965639929	39.0	35.0	40.0	31.0	41.0
48	36.823514536576184	39.0	35.0	40.0	32.0	41.0
49	37.033370121557965	39.0	35.0	40.0	32.0	41.0
50	36.689694919108376	39.0	35.0	40.0	31.0	41.0
51	35.98456658832016	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	13.0
11	15.0
12	15.0
13	23.0
14	17.0
15	27.0
16	52.0
17	100.0
18	181.0
19	275.0
20	465.0
21	704.0
22	1024.0
23	1357.0
24	1828.0
25	2812.0
26	3825.0
27	5484.0
28	7292.0
29	10439.0
30	15002.0
31	21543.0
32	30117.0
33	40582.0
34	52802.0
35	71446.0
36	90063.0
37	93854.0
38	53581.0
39	5181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.54631493726072	3.789056450648564	48.85763741186018	16.80699120023053
2	16.918924111054697	16.225371329100398	46.88322711795298	19.972477441891918
3	15.954262369958853	16.048160556096818	48.349920706020264	19.647656367924064
4	18.963709037161333	3.437379319267555	56.49083463529671	21.10807700827441
5	25.619306564835814	4.4798648179767	46.0881309948307	23.812697622356787
6	18.115488888061208	14.603226255422669	47.896504203855116	19.384780652661004
7	68.39767352051548	1.251649099145899	23.74389122708659	6.606786153252034
8	66.10040244880196	10.63695903177052	12.428081634573351	10.834556884854164
9	61.79206354496037	3.6042005226149567	15.985823138159713	18.617912794264956
10	32.64540006704213	23.064060518263098	24.895761251609894	19.394778163084876
11	25.18627714275073	24.70443634624319	29.021204523579424	21.08808198742666
12	19.542584493665302	19.447902189062724	35.15065856149547	25.858854755776502
13	20.516459626720405	23.806816733872154	37.779611743742244	17.897111895665198
14	17.362147073179816	26.85292093929551	29.58400555155873	26.200926435965943
15	12.009362374467534	26.33579481188018	39.946915179945385	21.7079276337069
16	13.79264379262419	26.218569101419842	28.343726170149786	31.64506093580618
17	14.830228550929474	27.027387297672934	32.948265824000686	25.194118327396904
18	14.816898537030976	23.08052700602007	35.80265306482504	26.29992139212392
19	15.0291986113262	25.806514848263273	32.274119974045675	26.890166566364847
20	17.007921556788798	26.168777578916625	38.16853450219259	18.654766362101988
21	20.278087613476643	28.936911788633026	29.856486718013358	20.928513879876974
22	15.740394058734394	24.091451736528356	32.11690422188984	28.05124998284741
23	16.243013994554296	27.693495933365615	29.89039984160807	26.173090230472017
24	21.189625328594644	22.434609420791293	28.249827984011826	28.125937266602236
25	15.009791679326911	28.98121448188392	26.78274233671223	29.226251502076934
26	15.399498556241879	22.825492475403184	35.37707276815381	26.397936200201126
27	17.47133556937782	26.01313006369002	32.46681708672546	24.048717280206695
28	13.082232463680613	27.114032388013182	35.434509445687056	24.36922570261915
29	20.529397581386597	19.938760347913362	33.13370984088276	26.39813222981728
30	18.351900605143427	25.148835486065234	32.62285666118437	23.876407247606966
31	24.782456133472643	26.304430073295475	25.317616985574183	23.5954968076577
32	26.009993589831552	26.714131971058187	26.320112442587828	20.955761996522433
33	18.680054182585906	21.44113132612075	29.308191881629476	30.570622609663868
34	25.15314813762063	21.537773926884874	31.093825655179984	22.21525228031451
35	20.860099543839084	19.174636904143476	32.046137530458104	27.919126021559336
36	19.62197648820784	23.284985895669116	35.46665830273638	21.626379313386668
37	21.698714241747645	22.30072119295783	37.17701670368359	18.82354786161093
38	21.317632667943474	27.446302587394904	26.04684715766858	25.18921758699304
39	27.2502729712405	20.129301134815446	31.06422518314067	21.556200710803388
40	25.738296541841542	18.870594969487993	29.352298545264215	26.03880994340625
41	24.38863263461844	21.44211147420152	29.24683461177315	24.92242127940689
42	24.09929292117453	18.404240512656653	33.66769451528737	23.828772050881447
43	22.548502627777005	18.838054053206356	33.741009591729124	24.87243372728752
44	20.290437479294372	19.041924854006943	33.42814632434668	27.239491342352
45	21.160220886171484	18.005124214166276	29.15685701795827	31.677797881703967
46	26.930352637676503	19.842901865613857	31.43197674304634	21.794768753663305
47	16.600768044036094	18.7110268619383	40.92921958649513	23.75898550753048
48	21.491510937472434	20.6807324450578	30.653739166913336	27.17401745055643
49	21.683227902071444	15.894277307415605	38.988918445798795	23.43357634471416
50	24.795786147371146	14.663799406814382	32.675588627929905	27.864825817884565
51	20.591538969707543	15.228364701339078	29.041787633275636	35.13830869567774
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	102.0
1	228.5
2	355.0
3	1126.5
4	1898.0
5	1297.0
6	696.0
7	732.5
8	769.0
9	925.0
10	1081.0
11	1068.5
12	1056.0
13	1090.5
14	1125.0
15	1087.5
16	1050.0
17	1052.0
18	1054.0
19	1108.0
20	1162.0
21	1681.0
22	2200.0
23	2170.5
24	2141.0
25	2309.0
26	2820.0
27	3163.0
28	3983.0
29	4803.0
30	5491.0
31	6179.0
32	7081.0
33	7983.0
34	9119.5
35	10256.0
36	11410.5
37	12565.0
38	14149.0
39	15733.0
40	18175.0
41	20617.0
42	23173.0
43	25729.0
44	30650.0
45	35571.0
46	69956.0
47	104341.0
48	93041.0
49	81741.0
50	75976.0
51	70211.0
52	51921.5
53	33632.0
54	26658.5
55	19685.0
56	16241.5
57	12798.0
58	10998.0
59	9198.0
60	7705.0
61	6212.0
62	5525.5
63	4839.0
64	3919.5
65	3000.0
66	2454.5
67	1909.0
68	1552.0
69	1195.0
70	966.5
71	738.0
72	564.5
73	391.0
74	306.5
75	173.5
76	125.0
77	90.0
78	55.0
79	40.0
80	25.0
81	22.5
82	20.0
83	19.0
84	18.0
85	12.0
86	6.0
87	3.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	510127.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.958906965104806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.47837279622979	20.89056665555137
2	13.573857977847267	8.404636125874383
3	5.751865169003707	5.342143759290377
4	2.997393608999259	3.7118411951523123
5	1.8623423151317713	2.88280412356712
6	1.225282533848652	2.2760044782832973
7	0.9173596674712743	1.9880316819147863
8	0.729319688319832	1.806315230281033
9	0.6315894337152911	1.759798666668143
>10	4.6048636602360205	24.559040359695178
>50	0.12411267119169202	2.676008606563863
>100	0.08700682104159851	5.016897195193741
>500	0.005118048296564619	1.1689588332425371
>1k	0.00703731640777635	4.835076759931687
>5k	0.0031987801853528867	7.180322701040613
>10k+	0.0012795120741411548	5.501553627749568
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGC	15104	2.9608313223961877	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCG	12673	2.4842833255248205	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC	9961	1.9526510065140639	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	7861	1.5409888125898061	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	7786	1.5262865913782253	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	5634	1.104430857413938	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTAGCGGTT	5011	0.9823044065497416	No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	4787	0.938393772531154	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTA	3310	0.6488580294710925	No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT	3170	0.6214138832094752	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT	2721	0.5333965855561458	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTC	2660	0.5214387789707269	No Hit
TGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCTT	1623	0.31815606701860516	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG	1541	0.30208163849394365	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1204	0.23601965784990794	No Hit
TTCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT	1155	0.22641420665834194	No Hit
TCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG	1129	0.22131743663832731	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGT	1112	0.21798493316370235	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATG	972	0.19054078690208517	No Hit
TTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG	834	0.16348869987277678	No Hit
TGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG	817	0.16015619639815185	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTAT	804	0.1576078113881445	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTTAGCGGT	653	0.12800733934882882	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCC	650	0.1274192505003656	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC	630	0.12349865817727743	No Hit
TGCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT	542	0.10624805195568947	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2461602698935754	0.0
2	0.0	0.0	0.0	5.605466873935314	0.0
3	0.0	0.0	0.0	6.99394464515699	0.0
4	0.0	0.0	0.0	9.33904694321218	0.0
5	0.0	0.0	0.0	13.720308864263213	0.0
6	0.0	0.0	0.0	15.068992623405544	0.0
7	0.0	0.0	0.0	16.852666100794508	0.0
8	0.0	0.0	0.0	19.203453257718177	0.0
9	0.0	0.0	0.0	19.942876969852605	0.0
10	0.0	0.0	0.0	24.095372328851443	0.0
11	0.0	0.0	0.0	27.012293017229045	0.0
12	0.0	0.0	0.0	32.50857139496635	0.0
13	0.0	0.0	0.0	33.59026281690638	0.0
14	0.0	0.0	0.0	34.01760738012299	0.0
15	0.0	0.0	0.0	35.18633595163557	0.0
16	0.0	0.0	0.0	36.4283795995899	0.0
17	0.0	0.0	0.0	38.12540798663862	0.0
18	0.0	0.0	0.0	39.87379613311979	0.0
19	0.0	0.0	0.0	42.1418587920263	0.0
20	0.0	0.0	0.0	43.46878326377549	0.0
21	0.0	0.0	0.0	45.05779933232313	0.0
22	0.0	0.0	0.0	46.70993693727248	0.0
23	0.0	0.0	0.0	48.18898039115749	0.0
24	0.0	0.0	0.0	49.318307009823044	0.0
25	0.0	0.0	0.0	50.32374291107901	0.0
26	0.0	0.0	0.0	51.171767030562975	0.0
27	0.0	0.0	0.0	52.03390528241007	0.0
28	0.0	0.0	0.0	52.863698647591676	0.0
29	0.0	0.0	0.0	53.78307754735585	0.0
30	0.0	0.0	0.0	54.73107677107858	0.0
31	0.0	0.0	0.0	55.60105620757184	0.0
32	0.0	0.0	0.0	56.38674290911871	0.0
33	0.0	0.0	0.0	57.18595565418023	0.0
34	0.0	0.0	0.0	57.96536940801016	0.0
35	0.0	0.0	0.0	58.7653662715363	0.0
36	0.0	0.0	0.0	59.50361380597381	0.0
37	0.0	0.0	0.0	60.179327892858055	0.0
38	0.0	0.0	0.0	60.931493530042516	0.0
39	0.0	0.0	0.0	61.677582249126196	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAT	20	7.029592E-4	45.000004	22
TCGTTGA	20	7.029592E-4	45.000004	24
ACCGGCG	30	2.1631186E-6	45.000004	20
CTCCGAC	20	7.029592E-4	45.000004	20
ACGTTGA	40	6.8030204E-9	45.000004	28
ACTTGCG	20	7.029592E-4	45.000004	1
ACAGGCG	20	7.029592E-4	45.000004	33
CTACGTA	80	0.0	45.000004	14
CCTCACG	40	6.8030204E-9	45.000004	24
GTCCGCG	40	6.8030204E-9	45.000004	1
TCTCCCG	20	7.029592E-4	45.000004	1
TTCGATA	20	7.029592E-4	45.000004	23
CGTCATA	20	7.029592E-4	45.000004	38
CGATAAG	20	7.029592E-4	45.000004	25
TACGTAT	80	0.0	45.000004	15
GTACGGC	20	7.029592E-4	45.000004	9
TTTAGCG	35	1.2101555E-7	45.0	1
TACTTGG	35	1.2101555E-7	45.0	2
TTCGTAC	55	1.8189894E-12	45.0	33
CGTTCAT	25	3.887745E-5	45.0	17
>>END_MODULE