SRR2933774.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933774.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440932
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC	12195	2.765732584616222	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG	11352	2.574546642112616	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	8800	1.9957725907849735	No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	7046	1.5979788266671506	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	5135	1.1645786651910046	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAGTTACT	4233	0.9600119746355447	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	3808	0.863625230194225	TruSeq Adapter, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	3337	0.7568060381192565	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTA	2547	0.5776400896283327	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	2267	0.5141382344669926	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCT	2154	0.48851070006259467	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTC	2018	0.4576669418413724	No Hit
TGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCTT	1104	0.2503787432075694	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG	1096	0.248564404488674	TruSeq Adapter, Index 27 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	900	0.20411310587573595	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGT	862	0.19549499696098266	No Hit
TGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG	752	0.17054783957617048	TruSeq Adapter, Index 27 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG	742	0.16827991617755117	TruSeq Adapter, Index 27 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATG	735	0.16669236979851768	No Hit
TTCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	675	0.15308482940680196	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTAT	659	0.1494561519690111	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGAGTTAC	574	0.13017880308074714	No Hit
TTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG	529	0.11997314778696036	TruSeq Adapter, Index 27 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCC	479	0.10863353079386391	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	468	0.1061388150553827	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTAGC	20	7.02855E-4	45.000004	43
TATGCGA	20	7.02855E-4	45.000004	31
ACGGGTT	40	6.7993824E-9	45.000004	20
ACGGGTA	20	7.02855E-4	45.000004	5
ACGGGAA	20	7.02855E-4	45.000004	5
CCGTAGG	20	7.02855E-4	45.000004	2
TCGCTAG	20	7.02855E-4	45.000004	1
TCCGTTC	20	7.02855E-4	45.000004	16
CTCGATC	20	7.02855E-4	45.000004	16
TTCGGAC	20	7.02855E-4	45.000004	13
ATACGGG	20	7.02855E-4	45.000004	25
ATACGAG	20	7.02855E-4	45.000004	18
TATACGA	20	7.02855E-4	45.000004	17
TACAAGT	20	7.02855E-4	45.000004	32
CGGACGA	20	7.02855E-4	45.000004	10
CGCGACC	25	3.8868806E-5	45.0	10
CCCGGTC	25	3.8868806E-5	45.0	38
AAGTGAT	25	3.8868806E-5	45.0	12
GCATCAC	35	1.2096461E-7	45.0	39
TACGGGA	30	2.1624473E-6	44.999996	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content