##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933774.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440932
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.898825669264195	31.0	16.0	33.0	16.0	34.0
2	28.11425571289904	31.0	25.0	33.0	16.0	34.0
3	29.011162718968006	31.0	28.0	34.0	16.0	34.0
4	32.50683552112344	35.0	32.0	37.0	26.0	37.0
5	33.78685602315096	35.0	33.0	37.0	28.0	37.0
6	32.935502526466664	35.0	33.0	37.0	26.0	37.0
7	33.56571081255159	35.0	32.0	35.0	29.0	37.0
8	33.42252093293297	35.0	33.0	37.0	28.0	37.0
9	34.71401259151071	37.0	32.0	39.0	28.0	39.0
10	33.59344978364011	35.0	31.0	39.0	27.0	39.0
11	34.23903459036767	37.0	32.0	39.0	27.0	39.0
12	34.334913319967704	37.0	32.0	39.0	27.0	39.0
13	34.67518120707955	37.0	34.0	39.0	27.0	39.0
14	34.458970997795575	37.0	32.0	39.0	25.0	40.0
15	34.84610325401649	37.0	32.0	39.0	27.0	40.0
16	34.29965845073617	36.0	32.0	39.0	27.0	40.0
17	34.32246015258588	36.0	32.0	38.0	27.0	40.0
18	34.263249208494734	36.0	32.0	39.0	27.0	40.0
19	33.91154418368365	36.0	32.0	39.0	25.0	40.0
20	34.97826422214763	37.0	33.0	39.0	27.0	40.0
21	34.461395407908704	37.0	32.0	39.0	25.0	40.0
22	35.37140420745149	37.0	34.0	39.0	28.0	40.0
23	36.19193208930175	38.0	34.0	39.0	30.0	40.0
24	36.33974853265356	38.0	35.0	40.0	31.0	40.0
25	34.36385429045748	37.0	32.0	39.0	25.0	40.0
26	35.06445438298876	37.0	33.0	39.0	27.0	40.0
27	35.428426605462974	37.0	34.0	39.0	30.0	40.0
28	35.48305180844212	38.0	34.0	39.0	29.0	40.0
29	36.03357660591656	38.0	34.0	40.0	30.0	41.0
30	33.89163862001397	37.0	32.0	39.0	24.0	40.0
31	34.67789817931109	37.0	33.0	39.0	26.0	40.0
32	35.09322752714704	37.0	33.0	39.0	29.0	40.0
33	35.784565420518355	38.0	34.0	39.0	30.0	40.0
34	36.13303411863961	38.0	35.0	40.0	30.0	41.0
35	36.419026062975696	38.0	35.0	40.0	31.0	41.0
36	36.27186051363929	38.0	35.0	40.0	30.0	41.0
37	36.52547331561329	38.0	35.0	40.0	31.0	41.0
38	35.73365734398955	38.0	34.0	40.0	30.0	41.0
39	35.74863244219063	38.0	34.0	40.0	30.0	41.0
40	35.287738245353026	37.0	34.0	40.0	28.0	41.0
41	34.89493618063556	37.0	33.0	39.0	27.0	40.0
42	35.88639518111636	38.0	34.0	40.0	30.0	41.0
43	35.93623733364782	38.0	34.0	40.0	30.0	41.0
44	36.374073553291666	38.0	35.0	40.0	31.0	41.0
45	36.5630777534858	38.0	35.0	40.0	31.0	41.0
46	36.30709043571344	38.0	35.0	40.0	31.0	41.0
47	36.030186060435625	38.0	35.0	40.0	30.0	41.0
48	36.15882040768191	38.0	35.0	40.0	31.0	41.0
49	36.33052488819138	38.0	35.0	40.0	31.0	41.0
50	36.08263859279889	38.0	35.0	40.0	31.0	41.0
51	35.33093084647973	37.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	10.0
11	8.0
12	19.0
13	18.0
14	23.0
15	34.0
16	40.0
17	109.0
18	189.0
19	354.0
20	629.0
21	853.0
22	1129.0
23	1532.0
24	2242.0
25	3113.0
26	4437.0
27	5919.0
28	8113.0
29	11066.0
30	15456.0
31	21567.0
32	29042.0
33	37844.0
34	46571.0
35	60336.0
36	73619.0
37	73252.0
38	39707.0
39	3697.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.42926346919707	4.111971914036632	46.86074043163118	16.598024185135124
2	17.555314651692324	16.10044179147805	45.6823274337086	20.661916123121024
3	17.95129407709125	16.070505202616275	46.87797664946069	19.100224070831786
4	18.870483430551648	3.8035343318244084	54.55467056144712	22.771311676176826
5	26.21265864124173	4.596627144321573	44.890368582910746	24.30034563152595
6	19.555623089274537	14.894813712772038	46.441854979906196	19.10770821804723
7	69.00587845744923	1.676448976259378	23.211515607848828	6.106156958442572
8	66.62546605825843	11.15478123610897	12.445683234603068	9.774069471029547
9	62.01681891992416	4.328331806264911	15.03746609454519	18.617383179265737
10	38.34627561619479	20.228969546324606	23.07158473415402	18.35317010332659
11	27.607885116072318	22.711892083133	28.737991345604314	20.94223145519037
12	23.607948617927484	17.491586004191124	34.606923516551305	24.29354186133009
13	22.577177433255013	21.591764716554934	37.82624078089138	18.004817069298667
14	18.605136392913195	25.304808904774433	30.940825342683226	25.14922935962915
15	14.455063365779758	23.51382979688478	40.26040296462946	21.770703872705997
16	16.43428011575481	23.644008599965527	29.16345377518529	30.75825750909437
17	16.7059773389094	25.2043398982156	32.79349196701532	25.29619079585968
18	16.83298104923208	22.604619306378307	34.47561075177125	26.08678889261836
19	18.039516297297542	24.63690546388105	30.205337784510988	27.118240454310417
20	19.344025836183356	25.395299048379343	37.08848529932053	18.17218981611677
21	22.738653579236708	27.170856277158386	29.475293242495443	20.615196901109467
22	17.308337793582684	23.54784864786407	31.794471709923528	27.34934184862972
23	18.226393185343774	27.237306432737928	29.986483176544226	24.549817205374072
24	23.59728937795397	21.689739007375287	27.00733900011793	27.705632614552812
25	18.54617038454909	28.25787196211661	24.52963268712636	28.66632496620794
26	21.420990084638902	22.909655003492603	28.91783767111482	26.751517240753675
27	25.207514990973667	25.06009997006341	26.073861729246232	23.658523309716692
28	18.254061850806927	26.064563243311895	31.358803625048758	24.32257128083242
29	28.140847114747853	20.126459408707014	27.202833997078912	24.52985947946622
30	24.229359629149165	24.495160251467347	31.581740495133037	19.69373962425045
31	23.856513022416152	25.758620376838152	27.212132483013253	23.17273411773244
32	27.025482387306887	29.043027042718606	24.285377337095063	19.646113232879447
33	20.273194052597677	21.642792993023868	27.945805702466593	30.138207251911858
34	24.78817595456896	22.962951203360156	30.497219525913295	21.751653316157594
35	23.79913456043109	23.021010042364807	26.118766612538895	27.06108878466521
36	25.646358168606497	24.977093973673945	25.73730189689113	23.63924596082843
37	25.51118993404879	23.58663013798046	30.967813631126795	19.934366296843958
38	20.879636769388476	29.150753404153022	26.719766313173004	23.249843513285494
39	25.147641813250114	20.74242740377201	33.2427675922818	20.86716319069607
40	22.285522484192573	22.77788865403282	33.566627053604634	21.36996180816997
41	19.302522837988626	24.408525577640088	28.335434942349387	27.953516642021896
42	20.973302005751453	23.66419311821324	29.40362686309907	25.95887801293624
43	26.015576097901715	19.721181497373745	25.47512995201074	28.788112452713797
44	23.58776409967977	18.928769061896165	30.746237515081692	26.737229323342376
45	21.212567924305787	19.44449484274219	28.483757132619086	30.859180100332935
46	26.931136773924326	21.44298894160551	29.620893924686797	22.004980359783367
47	17.193354077272684	20.354839294947976	39.939038219045116	22.512768408734228
48	20.703419121315758	22.446318253154683	29.493436629684393	27.356825995845163
49	20.64014405849428	18.90110039643301	36.96397630473633	23.494779240336378
50	23.53129280705415	17.328749104170257	30.54688704834306	28.593071040432537
51	19.995146643926955	17.850598278192557	28.497818257690525	33.65643682018996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	122.0
1	248.0
2	374.0
3	1007.0
4	1640.0
5	1142.0
6	644.0
7	671.5
8	699.0
9	895.0
10	1091.0
11	1093.5
12	1096.0
13	1053.0
14	1010.0
15	948.0
16	886.0
17	893.0
18	900.0
19	911.5
20	923.0
21	1193.0
22	1463.0
23	1639.0
24	1815.0
25	2008.0
26	2345.0
27	2489.0
28	2891.5
29	3294.0
30	4107.0
31	4920.0
32	5473.5
33	6027.0
34	7071.5
35	8116.0
36	8765.5
37	9415.0
38	10118.0
39	10821.0
40	12159.5
41	13498.0
42	15763.0
43	18028.0
44	21644.0
45	25260.0
46	53312.5
47	81365.0
48	73037.0
49	64709.0
50	62054.5
51	59400.0
52	45956.0
53	32512.0
54	27612.5
55	22713.0
56	19348.0
57	15983.0
58	14636.0
59	13289.0
60	12218.0
61	11147.0
62	9693.0
63	8239.0
64	6911.0
65	5583.0
66	4296.0
67	3009.0
68	2786.5
69	2564.0
70	2059.0
71	1554.0
72	1257.0
73	960.0
74	748.5
75	411.0
76	285.0
77	250.0
78	215.0
79	157.5
80	100.0
81	62.5
82	25.0
83	14.0
84	3.0
85	3.5
86	4.0
87	3.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	440932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.10599810010073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.32032382949868	22.57704183268352
2	12.431288948175169	7.982378787038301
3	5.0064142017372415	4.822077745478795
4	2.4970521323754276	3.206814040715918
5	1.527817399281271	2.452605125931266
6	0.9921289850203879	1.9111974784871661
7	0.7829638893174476	1.7596486000011413
8	0.6257862262752161	1.607319311348903
9	0.5615619094666567	1.6226555038582877
>10	5.051850219007189	28.625122509115364
>50	0.11528275822787995	2.5783215782572566
>100	0.07116219643696292	4.057457944906568
>500	0.006404597679326663	1.4686265932687323
>1k	0.006404597679326663	5.15527231650835
>5k	0.0021348658931088877	4.793599028215817
>10k+	0.0014232439287392587	5.379861604184636
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC	12195	2.765732584616222	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG	11352	2.574546642112616	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	8800	1.9957725907849735	No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	7046	1.5979788266671506	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	5135	1.1645786651910046	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAGTTACT	4233	0.9600119746355447	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	3808	0.863625230194225	TruSeq Adapter, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	3337	0.7568060381192565	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTA	2547	0.5776400896283327	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	2267	0.5141382344669926	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCT	2154	0.48851070006259467	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTC	2018	0.4576669418413724	No Hit
TGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCTT	1104	0.2503787432075694	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG	1096	0.248564404488674	TruSeq Adapter, Index 27 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	900	0.20411310587573595	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGT	862	0.19549499696098266	No Hit
TGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG	752	0.17054783957617048	TruSeq Adapter, Index 27 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG	742	0.16827991617755117	TruSeq Adapter, Index 27 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATG	735	0.16669236979851768	No Hit
TTCCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	675	0.15308482940680196	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTAT	659	0.1494561519690111	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGAGTTAC	574	0.13017880308074714	No Hit
TTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG	529	0.11997314778696036	TruSeq Adapter, Index 27 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCC	479	0.10863353079386391	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	468	0.1061388150553827	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.971805176308365	0.0
2	0.0	0.0	0.0	4.8959930329393195	0.0
3	0.0	0.0	0.0	6.104115827383814	0.0
4	0.0	0.0	0.0	8.1343608538278	0.0
5	0.0	0.0	0.0	12.308927453666325	0.0
6	0.0	0.0	0.0	13.488020828608493	0.0
7	0.0	0.0	0.0	15.098019649288325	0.0
8	0.0	0.0	0.0	17.065443197590557	0.0
9	0.0	0.0	0.0	17.743098709098003	0.0
10	0.0	0.0	0.0	21.782270282038954	0.0
11	0.0	0.0	0.0	24.29104714559161	0.0
12	0.0	0.0	0.0	29.30247747952065	0.0
13	0.0	0.0	0.0	30.279045294966117	0.0
14	0.0	0.0	0.0	30.69180735351483	0.0
15	0.0	0.0	0.0	31.761813612983406	0.0
16	0.0	0.0	0.0	32.85971533025501	0.0
17	0.0	0.0	0.0	34.25720972848421	0.0
18	0.0	0.0	0.0	35.56874982990575	0.0
19	0.0	0.0	0.0	37.568831475148095	0.0
20	0.0	0.0	0.0	38.66446526902107	0.0
21	0.0	0.0	0.0	39.91726615441837	0.0
22	0.0	0.0	0.0	41.26985566935491	0.0
23	0.0	0.0	0.0	42.44350602814039	0.0
24	0.0	0.0	0.0	43.37222065987499	0.0
25	0.0	0.0	0.0	44.228815327533496	0.0
26	0.0	0.0	0.0	44.986755327352064	0.0
27	2.2679233986192882E-4	0.0	0.0	45.753313436085385	0.0
28	2.2679233986192882E-4	0.0	0.0	46.50785155080602	0.0
29	2.2679233986192882E-4	0.0	0.0	47.32362359728938	0.0
30	2.2679233986192882E-4	0.0	0.0	48.15844620032114	0.0
31	2.2679233986192882E-4	0.0	0.0	48.91230393802219	0.0
32	2.2679233986192882E-4	0.0	0.0	49.631689240064226	0.0
33	2.2679233986192882E-4	0.0	0.0	50.29936588861774	0.0
34	2.2679233986192882E-4	0.0	0.0	50.98722705541898	0.0
35	2.2679233986192882E-4	0.0	0.0	51.66851124436421	0.0
36	2.2679233986192882E-4	0.0	0.0	52.335734308238	0.0
37	4.5358467972385764E-4	0.0	0.0	53.029265283535786	0.0
38	4.5358467972385764E-4	0.0	0.0	53.7350430451861	0.0
39	4.5358467972385764E-4	0.0	0.0	54.51452831729156	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTAGC	20	7.02855E-4	45.000004	43
TATGCGA	20	7.02855E-4	45.000004	31
ACGGGTT	40	6.7993824E-9	45.000004	20
ACGGGTA	20	7.02855E-4	45.000004	5
ACGGGAA	20	7.02855E-4	45.000004	5
CCGTAGG	20	7.02855E-4	45.000004	2
TCGCTAG	20	7.02855E-4	45.000004	1
TCCGTTC	20	7.02855E-4	45.000004	16
CTCGATC	20	7.02855E-4	45.000004	16
TTCGGAC	20	7.02855E-4	45.000004	13
ATACGGG	20	7.02855E-4	45.000004	25
ATACGAG	20	7.02855E-4	45.000004	18
TATACGA	20	7.02855E-4	45.000004	17
TACAAGT	20	7.02855E-4	45.000004	32
CGGACGA	20	7.02855E-4	45.000004	10
CGCGACC	25	3.8868806E-5	45.0	10
CCCGGTC	25	3.8868806E-5	45.0	38
AAGTGAT	25	3.8868806E-5	45.0	12
GCATCAC	35	1.2096461E-7	45.0	39
TACGGGA	30	2.1624473E-6	44.999996	4
>>END_MODULE