FastQCFastQC Report
Sat 14 Jan 2017
SRR2933773.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933773.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705336
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC106981.5167239443329137No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG85461.2116211280864722No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC76441.0837388138419137No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC71301.0108657434187394TruSeq Adapter, Index 16 (100% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC64050.9080778522576475TruSeq Adapter, Index 16 (100% over 23bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT54630.7745244819490286TruSeq Adapter, Index 16 (96% over 25bp)
TGAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCC49190.6973981194778092No Hit
TCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC47480.6731543548039516TruSeq Adapter, Index 16 (100% over 23bp)
TGAATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATG34780.4930983247700387No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTACCCTCT34680.49168056075402355No Hit
GAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT23590.3344505313779532TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTA20010.283694579604614No Hit
TGAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTACCCTC19240.272777796681298No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTC17830.25278732405548565No Hit
TGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTT17030.2414452119273651TruSeq Adapter, Index 16 (100% over 25bp)
TGAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC16430.23293862783127475No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCT16100.22826000657842505No Hit
TGAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTT16050.22755112457041748No Hit
TGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG15240.2160672360406955TruSeq Adapter, Index 16 (95% over 23bp)
TGAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGT13730.19465899939886805No Hit
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG13600.19281590617804847TruSeq Adapter, Index 16 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11250.1594984518016945No Hit
TCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG10350.14673857565755896TruSeq Adapter, Index 16 (95% over 23bp)
TGAATGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCT9720.13780666235666406No Hit
TTCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT8660.12277836378690439TruSeq Adapter, Index 16 (100% over 21bp)
TGAATGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTC8240.11682375491964114No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCC7350.10420565517710709No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGT7060.1000941395306634No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT351.2110286E-745.0000045
CCCTACG207.031431E-445.032
GGCGTAA207.031431E-445.08
ATAGCGT207.031431E-445.031
CGATTAC207.031431E-445.010
TCGGCGT551.8189894E-1245.04
GGGCGTA502.1827873E-1145.07
TAGGGCG253.889268E-545.05
CGCACGT207.031431E-445.025
GACGATA207.031431E-445.023
AATAGCG207.031431E-445.030
TATCGAC207.031431E-445.015
AGTACGA207.031431E-445.017
GTACGAA207.031431E-445.018
TCCGTCG302.1643045E-644.99999614
TCGCGGT302.1643045E-644.99999630
GCCGATT1050.042.8571479
TTTGTCG1600.042.18751
CGATTGA2050.041.70731420
TGGGACG1250.041.3999986
Produced by FastQC (version 0.11.5)