SRR2933771.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933771.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	709222
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGC	11285	1.5911801946358124	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG	9621	1.3565569031981524	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	8290	1.1688864699628607	No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	7590	1.0701867680359607	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	6793	0.9578101074134756	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	5814	0.8197715242899967	TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	4470	0.6302680965903483	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCAATCCT	4079	0.5751372630854655	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT	2433	0.3430519639830688	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTA	2371	0.334309990383829	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTC	1954	0.2755131679502328	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT	1900	0.2678991909444433	No Hit
TGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCTT	1679	0.23673828505037914	TruSeq Adapter, Index 20 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	1579	0.22263832763225055	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1552	0.21883133912935582	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	1066	0.15030554607725083	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCC	958	0.13507759206567196	No Hit
CCTGTCTCTTATACACATCTGACGCGTCACTCCTCGTATGCCGTCTTCTGC	838	0.11815764316391764	TruSeq Adapter, Index 27 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	816	0.11505565253192936	No Hit
TTCCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT	792	0.11167166275157848	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGT	781	0.11012066743558435	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	765	0.10786467424868376	No Hit
GCTGTCTCTTATACACATCTGACGCGTCACTCCTCGTATGCCGTCTTCTGC	713	0.1005326963912569	TruSeq Adapter, Index 27 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTC	35	1.2110468E-7	45.000004	5
GATAACG	25	3.889289E-5	45.000004	17
TCTACGG	35	1.2110468E-7	45.000004	2
ACGGTTA	35	1.2110468E-7	45.000004	34
GTCGACT	20	7.031458E-4	45.0	9
GTCGACC	20	7.031458E-4	45.0	9
GGTCGAC	40	6.8084773E-9	45.0	8
TGGACCG	20	7.031458E-4	45.0	19
CCGTGCG	40	6.8084773E-9	45.0	1
GTTCGAA	20	7.031458E-4	45.0	9
AACGATA	20	7.031458E-4	45.0	21
TCCCGAT	20	7.031458E-4	45.0	11
GCTCGAC	20	7.031458E-4	45.0	20
ACGACTT	20	7.031458E-4	45.0	22
GCCGATC	30	2.1643227E-6	44.999996	9
CTCTACG	30	2.1643227E-6	44.999996	1
CGTAGGG	85	0.0	42.35294	3
CGGGACG	80	0.0	42.1875	6
CCTTGCG	80	0.0	42.1875	1
ACGGGAC	75	0.0	42.000004	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content