FastQCFastQC Report
Sat 14 Jan 2017
SRR2933770.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933770.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265656
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC120374.531047670671846No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG107934.0627729093263465No Hit
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC84833.1932273315867135No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC50751.9103652844279821Illumina PCR Primer Index 2 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGATTACCT41641.5674405998735208No Hit
GAACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT25270.9512301623151744TruSeq Adapter, Index 10 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC24630.9271388562652453RNA PCR Primer, Index 10 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGATTACCTCGTA24250.9128346432980997No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTC19860.7475833408618664No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT19710.7419369410064143TruSeq Adapter, Index 10 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCT19300.7265034480681785No Hit
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC15230.5732977986569097Illumina PCR Primer Index 2 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT10350.3896015900261993Illumina PCR Primer Index 2 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGATTACCTCGT9040.34028969795525044No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATG6830.25709940675158854No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTAT5650.2126810612220315No Hit
CGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG5540.20854036799469994TruSeq Adapter, Index 10 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGATTACC5260.19800042159785589No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.18407263528774054No Hit
TCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG3820.1437949829855151TruSeq Adapter, Index 10 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT3770.14191284970036439No Hit
TGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG3240.12196223687776674TruSeq Adapter, Index 10 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT3110.11706869033637486No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC2950.11104586382389256No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTGATTACCTCG2850.10728159725359111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT302.1591804E-645.0000046
CAAGTGG351.2072087E-745.00000431
CGACGTT302.1591804E-645.00000427
TTTCGAC302.1591804E-645.00000424
TATGGCC302.1591804E-645.00000410
GGACTCC302.1591804E-645.0000048
GGGTACC302.1591804E-645.0000047
AGTGGCG302.1591804E-645.00000433
CCTCGAA302.1591804E-645.00000442
ACGGGAT302.1591804E-645.0000045
ATCCGGC302.1591804E-645.0000046
GATGCCA650.045.0000049
CATCGGT302.1591804E-645.00000428
CTCGAAT302.1591804E-645.00000443
GTCCCCC351.2072087E-745.0000049
TTTCTCG302.1591804E-645.0000041
TCGACGT302.1591804E-645.00000426
TTCGACG302.1591804E-645.00000425
GTCATAA207.0234854E-445.040
TATCACG207.0234854E-445.016
Produced by FastQC (version 0.11.5)