SRR2933769.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933769.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	363482
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC	9392	2.5838968642188607	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG	7472	2.0556726330327226	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	6572	1.8080675246642202	No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	4062	1.1175243891031743	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCATGCACT	3083	0.8481850545556588	No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	2693	0.7408895075959745	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	2340	0.6437732817581063	TruSeq Adapter, Index 16 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT	1943	0.5345519172888892	No Hit
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	1856	0.5106167568132672	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTA	1839	0.5059397714329733	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTC	1395	0.3837879179711788	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT	1334	0.3670057939595358	No Hit
TGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT	802	0.22064366323504328	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	706	0.19423245167573636	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCATGCACTCGT	644	0.1771752108770173	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATG	584	0.16066820365245046	No Hit
CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	582	0.16011797007829823	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT	530	0.14581189715034032	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTAT	464	0.1276541892033168	No Hit
TGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	459	0.12627860526793622	No Hit
TCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	452	0.12435278775840344	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT	378	0.10399414551477103	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	35	1.208864E-7	45.000004	2
CTACCTT	35	1.208864E-7	45.000004	17
CGGGTCA	25	3.885525E-5	45.0	6
ACCGGTG	20	7.026914E-4	45.0	17
GTTAGGT	20	7.026914E-4	45.0	39
AGGGTGT	20	7.026914E-4	45.0	6
TCGGCGT	25	3.885525E-5	45.0	4
AACGGAA	40	6.7921064E-9	45.0	13
AGACGGT	25	3.885525E-5	45.0	34
CGTACCA	20	7.026914E-4	45.0	21
CGTACAT	20	7.026914E-4	45.0	35
TCGTACC	20	7.026914E-4	45.0	20
TCGTACA	20	7.026914E-4	45.0	34
TGCTACG	50	2.1827873E-11	45.0	4
GCGGATG	20	7.026914E-4	45.0	24
CCAACGG	20	7.026914E-4	45.0	2
CGGTAGT	20	7.026914E-4	45.0	12
CGGTAGG	20	7.026914E-4	45.0	31
TAAGCGT	20	7.026914E-4	45.0	21
GCCAACG	20	7.026914E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content