##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933769.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	363482
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.086436192163575	31.0	16.0	33.0	16.0	34.0
2	28.38448671461035	31.0	26.0	33.0	16.0	34.0
3	29.301726082722116	31.0	28.0	34.0	16.0	34.0
4	32.66044260788705	35.0	32.0	37.0	28.0	37.0
5	33.82898740515349	35.0	33.0	37.0	28.0	37.0
6	33.01324412212984	35.0	33.0	37.0	26.0	37.0
7	33.48216417869386	35.0	32.0	35.0	29.0	37.0
8	33.6256238273147	35.0	35.0	37.0	28.0	37.0
9	34.73643811798108	37.0	33.0	39.0	28.0	39.0
10	33.445174726671475	35.0	31.0	38.0	27.0	39.0
11	33.93276695957434	35.0	32.0	39.0	27.0	39.0
12	34.140799269289815	37.0	32.0	39.0	26.0	39.0
13	34.49604106943397	37.0	33.0	39.0	27.0	39.0
14	34.246372585162405	37.0	32.0	39.0	25.0	40.0
15	34.79278203597427	37.0	32.0	39.0	27.0	40.0
16	34.385350581321774	36.0	32.0	39.0	27.0	40.0
17	34.37234305962881	36.0	32.0	38.0	27.0	40.0
18	34.297139885881556	36.0	32.0	39.0	27.0	40.0
19	34.11903753143209	36.0	32.0	39.0	25.0	40.0
20	35.08770448055199	37.0	33.0	39.0	27.0	40.0
21	34.40034444621742	37.0	32.0	39.0	25.0	40.0
22	35.3734545314486	37.0	34.0	39.0	28.0	40.0
23	36.165999966985986	38.0	34.0	39.0	30.0	40.0
24	36.2736779262797	38.0	35.0	40.0	31.0	40.0
25	34.198480254868194	37.0	32.0	39.0	24.0	40.0
26	34.778525484067984	37.0	33.0	39.0	27.0	40.0
27	35.51714802933846	37.0	34.0	39.0	30.0	40.0
28	35.631992780935505	38.0	34.0	40.0	29.0	40.0
29	36.20735552241927	38.0	35.0	40.0	30.0	41.0
30	34.0640581927028	37.0	32.0	39.0	24.0	40.0
31	34.55201908210035	37.0	32.0	39.0	25.0	40.0
32	35.197308257355246	37.0	34.0	39.0	29.0	40.0
33	36.06758243874525	38.0	35.0	40.0	30.0	40.0
34	36.29615496778383	38.0	35.0	40.0	30.0	41.0
35	36.51069654068152	38.0	35.0	40.0	31.0	41.0
36	36.43721559802136	38.0	35.0	40.0	31.0	41.0
37	36.713174242465925	38.0	35.0	40.0	31.0	41.0
38	36.158470020523716	38.0	35.0	40.0	30.0	41.0
39	36.12315602973462	38.0	35.0	40.0	30.0	41.0
40	35.61769496151116	38.0	34.0	40.0	29.0	41.0
41	35.145176377372195	37.0	33.0	39.0	28.0	41.0
42	36.06825097253784	38.0	35.0	40.0	31.0	41.0
43	36.220610649220596	38.0	35.0	40.0	31.0	41.0
44	36.66378252568215	38.0	35.0	40.0	32.0	41.0
45	36.90284525781194	39.0	35.0	40.0	33.0	41.0
46	36.68896946753897	39.0	35.0	40.0	32.0	41.0
47	36.57041889282	38.0	35.0	40.0	31.0	41.0
48	36.63651570091504	38.0	35.0	40.0	32.0	41.0
49	36.84855095988247	39.0	35.0	40.0	32.0	41.0
50	36.709019978981075	39.0	35.0	40.0	32.0	41.0
51	35.98760323757435	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	11.0
11	7.0
12	11.0
13	11.0
14	15.0
15	34.0
16	49.0
17	111.0
18	200.0
19	359.0
20	643.0
21	753.0
22	1035.0
23	1293.0
24	1754.0
25	2369.0
26	3363.0
27	4462.0
28	5957.0
29	8326.0
30	11642.0
31	16564.0
32	23461.0
33	30263.0
34	37468.0
35	48712.0
36	59705.0
37	63037.0
38	37980.0
39	3882.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.38642353679137	3.7121508080180035	47.782283579379445	17.11914207581118
2	18.569557777276454	14.393284949461046	46.77920777369994	20.257949499562564
3	18.30599589525754	14.403739387369937	47.28624801228121	20.004016705091313
4	17.917255875118986	3.93967239092995	54.86021316048662	23.282858573464434
5	24.731348457420175	4.6582774387727595	46.13130774013568	24.47906636367138
6	20.075822186518177	14.049388965615902	48.24145349700948	17.633335350856438
7	73.32550167546124	1.6889419558602625	19.879113683758757	5.106442684919749
8	71.99641247709653	9.75536615293192	10.360072850925217	7.888148519046336
9	67.1419767691385	4.250554360325958	12.391810323482318	16.215658547053224
10	39.09464567708992	22.559301423454254	20.39715859382308	17.94889430563274
11	29.96406974760786	23.447928645710103	26.086848867344187	20.50115273933785
12	25.263149206838303	18.680429842468126	32.14134400052823	23.915076950165346
13	23.590989374989682	22.06986865924585	35.653209787554815	18.68593217820965
14	20.47281571026901	26.20679978650937	29.45510369151705	23.86528081170457
15	15.855530672770591	23.587412856757695	39.362884544489134	21.19417192598258
16	17.239368111763444	23.514506908182522	30.211674855976362	29.034450124077672
17	17.551625665094832	24.762161537572698	32.04312730754205	25.64308548979042
18	17.42011984087245	23.143924595991	33.70950968686207	25.72644587627448
19	20.29509026581784	24.918152755844854	29.979476287684122	24.807280690653183
20	21.766689959887973	24.96162120820288	34.319718720596896	18.951970111312253
21	24.23338707281241	27.357063073274606	28.269900572793148	20.139649281119834
22	19.893419756686715	23.2924876610121	29.77203823022873	27.042054352072455
23	20.030703033437693	27.52323361266858	27.754331713812512	24.691731640081212
24	22.764538546613036	21.9345112000044	27.976350960982938	27.324599292399625
25	18.22291062556055	28.228357937944658	25.852724481542417	27.69600695495238
26	18.35606715050539	23.52386087894311	31.070039231653833	27.050032738897663
27	19.91515398286573	26.35371215080802	27.54414248848636	26.18699137783989
28	15.616729301588524	26.107757743161976	34.08889573624003	24.18661721900947
29	19.767966501780005	20.829642183106728	32.98402671934236	26.418364595770903
30	21.08357497757798	25.550921366119915	28.35216049212891	25.01334316417319
31	21.391155545529077	26.424142048299505	25.43344649803842	26.751255908133004
32	22.010168316450333	29.114233992329748	26.92567995113926	21.949917740080664
33	21.519635085093622	22.524911825069744	24.397081561122697	31.55837152871394
34	20.68465563631762	23.675725345409127	29.355511414595497	26.284107603677757
35	17.533192840360734	23.747255710048915	27.44565067871311	31.27390077087724
36	20.19577310568336	25.46150841032018	27.82118509307201	26.521533390924446
37	16.88941955860263	25.02407271886916	31.83211273185467	26.25439499067354
38	21.334756604178473	28.830313468067192	25.04690741219648	24.788022515557852
39	21.16886118157158	22.233563147556136	31.590009959227693	25.00756571164459
40	23.854276140221526	23.431421638485535	28.28640758001772	24.427894641275223
41	17.735403678861676	25.12449034615194	25.07854584271023	32.06156013227615
42	18.168162384932405	24.687329771488	29.61081979300213	27.53368805057747
43	21.105859437331148	21.63518413566559	27.11881193566669	30.140144491336574
44	20.434849593652505	20.518485096923644	30.11400839656434	28.93265691285951
45	19.326128941735767	21.03405395590428	28.26192218596794	31.37789491639201
46	23.832817030829588	22.91942929773689	29.176410386208946	24.07134328522458
47	16.398336093671766	21.417566757088384	36.86097248281895	25.32312466642089
48	18.809459615606823	23.123841070534443	29.012165664324506	29.054533649534225
49	18.86008110442883	20.41916793678917	35.30793821977429	25.412812739007705
50	21.76531437595259	18.822114987812327	29.884285879355787	29.528284756879298
51	18.775345134009385	19.81803775702786	27.396129657039413	34.010487451923346
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	52.0
1	193.5
2	335.0
3	835.5
4	1336.0
5	972.5
6	609.0
7	636.5
8	664.0
9	680.5
10	697.0
11	705.0
12	713.0
13	673.5
14	634.0
15	643.0
16	652.0
17	697.5
18	743.0
19	755.0
20	767.0
21	876.0
22	985.0
23	1018.0
24	1051.0
25	1272.5
26	1892.5
27	2291.0
28	2556.0
29	2821.0
30	3344.5
31	3868.0
32	4768.0
33	5668.0
34	6433.5
35	7199.0
36	7782.0
37	8365.0
38	9592.0
39	10819.0
40	11920.0
41	13021.0
42	15684.5
43	18348.0
44	21108.0
45	23868.0
46	43582.5
47	63297.0
48	55597.0
49	47897.0
50	45076.0
51	42255.0
52	33882.5
53	25510.0
54	22100.0
55	18690.0
56	16545.0
57	14400.0
58	13153.5
59	11907.0
60	10897.5
61	9888.0
62	8397.5
63	6907.0
64	6275.5
65	5644.0
66	4705.0
67	3766.0
68	3080.5
69	2395.0
70	1993.0
71	1591.0
72	1342.0
73	1093.0
74	892.0
75	472.5
76	254.0
77	193.0
78	132.0
79	109.0
80	86.0
81	69.5
82	53.0
83	28.5
84	4.0
85	8.5
86	13.0
87	11.0
88	9.0
89	4.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	363482.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.2606464521493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.56541232997692	23.08746465379672
2	11.881152621375492	7.665873283244433
3	4.550678124633676	4.404234542890105
4	2.3135767793722892	2.985499300769266
5	1.387786235873568	2.2385440553338127
6	0.8767123032886107	1.696998339398602
7	0.7027115872820635	1.5868951052594713
8	0.5450255498179598	1.4066301256052391
9	0.4670416039201142	1.3560357656260396
>10	5.536797600845622	35.20273070430965
>50	0.11226133432373282	2.4903497662225362
>100	0.04541870777983084	2.6559491789631333
>500	0.005141740503377077	1.0638172624804971
>1k	0.007712610755065614	5.679866335151199
>5k	0.0025708702516885383	6.479111580949307
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC	9392	2.5838968642188607	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG	7472	2.0556726330327226	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	6572	1.8080675246642202	No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	4062	1.1175243891031743	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCATGCACT	3083	0.8481850545556588	No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	2693	0.7408895075959745	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	2340	0.6437732817581063	TruSeq Adapter, Index 16 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT	1943	0.5345519172888892	No Hit
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	1856	0.5106167568132672	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTA	1839	0.5059397714329733	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTC	1395	0.3837879179711788	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT	1334	0.3670057939595358	No Hit
TGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT	802	0.22064366323504328	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	706	0.19423245167573636	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCATGCACTCGT	644	0.1771752108770173	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATG	584	0.16066820365245046	No Hit
CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	582	0.16011797007829823	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT	530	0.14581189715034032	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTAT	464	0.1276541892033168	No Hit
TGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	459	0.12627860526793622	No Hit
TCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	452	0.12435278775840344	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT	378	0.10399414551477103	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7208059821394182	0.0
2	0.0	0.0	0.0	3.294798642023539	0.0
3	0.0	0.0	0.0	4.130603441160773	0.0
4	0.0	0.0	0.0	5.751041317039083	0.0
5	0.0	0.0	0.0	9.188625571555125	0.0
6	0.0	0.0	0.0	10.182347406474047	0.0
7	0.0	0.0	0.0	11.420648065103636	0.0
8	0.0	0.0	0.0	13.050990145316687	0.0
9	0.0	0.0	0.0	13.61360397488734	0.0
10	0.0	0.0	0.0	16.89052002575093	0.0
11	0.0	0.0	0.0	19.018273256997595	0.0
12	0.0	0.0	0.0	23.597041944305357	0.0
13	0.0	0.0	0.0	24.46145888929851	0.0
14	0.0	0.0	0.0	24.807280690653183	0.0
15	0.0	0.0	0.0	25.66812111741434	0.0
16	0.0	0.0	0.0	26.602142609537747	0.0
17	0.0	0.0	0.0	27.74250169196824	0.0
18	0.0	0.0	0.0	28.93678366466565	0.0
19	0.0	0.0	0.0	30.660940569271656	0.0
20	0.0	0.0	0.0	31.609543251110097	0.0
21	0.0	0.0	0.0	32.74082347956708	0.0
22	0.0	0.0	0.0	33.96041619667549	0.0
23	0.0	0.0	0.0	35.06005799461872	0.0
24	0.0	0.0	0.0	35.91154445061929	0.0
25	0.0	0.0	0.0	36.679945636922874	0.0
26	0.0	0.0	0.0	37.349579896666135	0.0
27	0.0	0.0	0.0	37.99417852878547	0.0
28	0.0	0.0	0.0	38.631624124440826	0.0
29	0.0	0.0	0.0	39.331796347549535	0.0
30	0.0	0.0	0.0	40.10239846814973	0.0
31	0.0	0.0	0.0	40.78798950154341	0.0
32	0.0	0.0	0.0	41.473305418150005	0.0
33	0.0	0.0	0.0	42.126157553881626	0.0
34	0.0	0.0	0.0	42.784512025354765	0.0
35	0.0	0.0	0.0	43.46762700766475	0.0
36	0.0	0.0	0.0	44.123230311267136	0.0
37	0.0	0.0	0.0	44.7455444836333	0.0
38	0.0	0.0	0.0	45.38354031286281	0.0
39	0.0	0.0	0.0	46.029514528917524	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	35	1.208864E-7	45.000004	2
CTACCTT	35	1.208864E-7	45.000004	17
CGGGTCA	25	3.885525E-5	45.0	6
ACCGGTG	20	7.026914E-4	45.0	17
GTTAGGT	20	7.026914E-4	45.0	39
AGGGTGT	20	7.026914E-4	45.0	6
TCGGCGT	25	3.885525E-5	45.0	4
AACGGAA	40	6.7921064E-9	45.0	13
AGACGGT	25	3.885525E-5	45.0	34
CGTACCA	20	7.026914E-4	45.0	21
CGTACAT	20	7.026914E-4	45.0	35
TCGTACC	20	7.026914E-4	45.0	20
TCGTACA	20	7.026914E-4	45.0	34
TGCTACG	50	2.1827873E-11	45.0	4
GCGGATG	20	7.026914E-4	45.0	24
CCAACGG	20	7.026914E-4	45.0	2
CGGTAGT	20	7.026914E-4	45.0	12
CGGTAGG	20	7.026914E-4	45.0	31
TAAGCGT	20	7.026914E-4	45.0	21
GCCAACG	20	7.026914E-4	45.0	1
>>END_MODULE