FastQCFastQC Report
Sat 14 Jan 2017
SRR2933768.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933768.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267049
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC112114.1981059655718616No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG90693.396005976431292No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC86473.237982542529648No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC52231.9558208418679717TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC43661.6349059535890418TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACCAGTAT35141.3158633808776667No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT30111.1275084347816318TruSeq Adapter, Index 15 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT27101.0147950376148196TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTC24160.9047028822425847No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTA22830.8548992881456212No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT20230.7575388786327603No Hit
TCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC20200.7564154892922272TruSeq Adapter, Index 21 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTT9300.34825069556523336TruSeq Adapter, Index 21 (95% over 23bp)
AGGCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC8060.30181726948986887No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTACCAGTATCGT8020.3003194170358249No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATG7550.28271965070080773No Hit
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG7060.26437095813876854TruSeq Adapter, Index 15 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG5890.22055877385798112TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTAT5790.21681414272287108No Hit
TGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG5590.20932488045265102TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5520.206703638658074No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT4470.1673850117394186No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTACCAGTA4300.16101913880973157No Hit
TTCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT3950.14791292983684642No Hit
ACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC3750.14042366756662636TruSeq Adapter, Index 15 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG3430.12844084793427424TruSeq Adapter, Index 15 (95% over 22bp)
TGCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT3410.12769192170725224TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCC3160.11833034386947713No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC2800.10484967178308101No Hit
CGTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT2760.103351819329037TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTACCAGTATCG2740.10260289310201498No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCA253.882736E-545.00000432
GTTTGCG253.882736E-545.0000041
AAGTACG253.882736E-545.00000415
CAACGGA253.882736E-545.00000437
GGCACGT502.1827873E-1145.00000411
ATAATCC351.2072451E-745.00000418
GATCCGG502.1827873E-1145.0000045
CCATGGG253.882736E-545.0000043
ATACTCG253.882736E-545.00000410
ATCCGGC502.1827873E-1145.0000046
GACCAAG253.882736E-545.00000442
TCTCGCG253.882736E-545.0000041
GCGATTT253.882736E-545.0000049
CTCGCGG253.882736E-545.0000042
GCGATCC253.882736E-545.0000049
ACTCGAC253.882736E-545.00000411
TCGTAGG253.882736E-545.0000042
CTCGACA253.882736E-545.00000412
CCGAAGG253.882736E-545.00000415
AACTCGA253.882736E-545.00000410
Produced by FastQC (version 0.11.5)