##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933768.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	267049
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.34557328430363	30.0	16.0	33.0	16.0	34.0
2	27.416343068126075	31.0	25.0	33.0	16.0	34.0
3	28.228931769076087	31.0	25.0	34.0	16.0	34.0
4	32.08702148294882	35.0	28.0	35.0	19.0	37.0
5	33.72990350085565	35.0	33.0	37.0	28.0	37.0
6	32.57289860662276	35.0	32.0	37.0	25.0	37.0
7	33.576253796119815	35.0	32.0	35.0	30.0	37.0
8	33.20244973768859	35.0	33.0	37.0	28.0	37.0
9	34.748720272309576	37.0	32.0	39.0	29.0	39.0
10	34.03931862691866	35.0	32.0	39.0	27.0	39.0
11	34.46554003197915	37.0	32.0	39.0	27.0	39.0
12	34.40579444221847	37.0	33.0	39.0	27.0	39.0
13	34.806690906912216	37.0	34.0	39.0	29.0	39.0
14	34.37063610049092	37.0	32.0	39.0	25.0	40.0
15	35.075502997577225	37.0	33.0	39.0	27.0	40.0
16	34.458252979790224	36.0	32.0	39.0	27.0	40.0
17	34.42933693816491	36.0	32.0	38.0	27.0	40.0
18	34.36780141472164	36.0	32.0	39.0	27.0	40.0
19	34.00480810637748	36.0	32.0	39.0	27.0	40.0
20	34.958644293743845	37.0	33.0	39.0	27.0	40.0
21	34.3897674209602	37.0	32.0	39.0	26.0	40.0
22	35.56863347175987	37.0	34.0	39.0	30.0	40.0
23	36.277795460758135	38.0	34.0	39.0	31.0	40.0
24	36.423558972323434	38.0	35.0	40.0	31.0	40.0
25	34.28664027949927	37.0	32.0	39.0	25.0	40.0
26	35.02492800946643	37.0	33.0	39.0	27.0	40.0
27	35.69561016892031	37.0	34.0	39.0	30.0	40.0
28	35.90504738830702	38.0	34.0	40.0	30.0	40.0
29	36.42273889810484	38.0	35.0	40.0	30.0	41.0
30	33.998801718036766	37.0	32.0	39.0	24.0	40.0
31	34.75257724237874	37.0	33.0	39.0	26.0	40.0
32	35.352085197847586	37.0	34.0	39.0	30.0	40.0
33	36.00760160120427	38.0	35.0	39.0	30.0	40.0
34	36.35004437387895	38.0	35.0	40.0	31.0	40.0
35	36.50746866679898	38.0	35.0	40.0	31.0	41.0
36	36.49988953338151	38.0	35.0	40.0	31.0	41.0
37	36.68816584222371	38.0	35.0	40.0	31.0	41.0
38	36.04505914644878	38.0	35.0	40.0	30.0	41.0
39	36.23658205048512	38.0	35.0	40.0	31.0	41.0
40	35.813150395620276	38.0	34.0	40.0	30.0	41.0
41	35.3575898056162	37.0	34.0	39.0	29.0	40.0
42	36.22769229617037	38.0	35.0	40.0	31.0	41.0
43	36.35420465907006	38.0	35.0	40.0	31.0	40.0
44	36.82041872465353	39.0	35.0	40.0	32.0	41.0
45	37.0906987107235	39.0	35.0	40.0	33.0	41.0
46	36.89387715363098	39.0	35.0	40.0	32.0	41.0
47	36.63691307587746	38.0	35.0	40.0	31.0	41.0
48	36.7967489112485	38.0	35.0	40.0	32.0	41.0
49	36.96781115076259	39.0	35.0	40.0	33.0	41.0
50	36.7791940804871	39.0	35.0	40.0	32.0	41.0
51	36.08196623091642	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	3.0
11	10.0
12	5.0
13	8.0
14	9.0
15	11.0
16	24.0
17	51.0
18	86.0
19	152.0
20	286.0
21	450.0
22	571.0
23	756.0
24	1033.0
25	1576.0
26	2166.0
27	2961.0
28	4050.0
29	5685.0
30	8447.0
31	12241.0
32	17492.0
33	23630.0
34	29853.0
35	38705.0
36	45642.0
37	44417.0
38	24566.0
39	2158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.68240659953791	4.080150084815895	43.015326775236005	16.222116540410187
2	16.911877595497455	22.0079461072687	40.410186894539954	20.669989402693886
3	16.81339379664406	22.240487700759036	44.28737797183289	16.658740530764014
4	18.120270062797463	3.0657295103145863	57.023242925455634	21.79075750143232
5	28.655040835202527	3.3810274518908514	43.675505244355904	24.288426468550718
6	17.07739029166932	18.821265011290063	45.08386101427079	19.01748368276983
7	64.19833064343997	1.2072690779594755	28.66964489662946	5.9247553819710985
8	59.42280255683414	13.868615871993528	14.546768570561957	12.161813000610374
9	55.50516946328202	3.762230901445053	17.00998693123734	23.72261270403559
10	34.75055139693464	22.72317065407472	24.296290193934446	18.229987755056186
11	29.002542604540736	22.772974248171685	27.86080457144569	20.363678575841888
12	22.552041011200192	16.25207358949107	34.50677591003898	26.689109489269757
13	19.941658646914988	23.86827885519137	39.849990076727494	16.340072421166152
14	18.076457878516678	29.203629296496146	26.917906451624983	25.80200637336219
15	11.457447884096176	25.447764267980784	43.107819164273224	19.986968683649817
16	12.219105856977558	26.217285966245896	28.532216933970922	33.03139124280563
17	13.802710364015592	26.777108320944848	32.938898853768414	26.481282461271157
18	12.798025830465571	23.908346408337046	35.56762991061566	27.72599785058173
19	15.147407404633606	26.418372658201307	30.021082273290666	28.413137663874423
20	18.486494987811223	25.541005583245024	38.88986665368528	17.082632775258475
21	20.937356065740744	30.846024512355406	28.630700732824316	19.58591868907953
22	14.530292193567472	24.4546880909496	31.586338087766663	29.428681627716262
23	16.587592539196926	29.874292732794355	28.212799898146034	25.325314829862684
24	22.50410973267078	21.414796535467275	26.67375650161581	29.407337230246135
25	13.993686551906205	32.20270437260578	23.882508453504787	29.921100621983232
26	14.508947796097345	23.48782433186419	33.365412340057446	28.63781553198102
27	18.382019779141658	31.46913113323772	24.942613527854437	25.20623555976618
28	11.361959790150872	24.134896592011206	34.1772483701493	30.325895247688628
29	17.219311811689987	21.470966002493924	30.135667986025037	31.17405419979105
30	16.465892027305852	33.97840845687495	27.219723721114853	22.335975794704343
31	22.556160105448814	23.672060183711604	21.945410767312364	31.826368943527218
32	18.388385652071342	31.647001112155447	25.63349797228224	24.33111526349097
33	16.456530449468076	31.873925758943116	21.29721511782482	30.37232867376399
34	23.870151170758923	21.98472939423102	30.38580934585039	23.75931008915967
35	13.749162138783518	27.004781893959535	29.24819040700396	29.99786556025299
36	18.3835176315957	29.40920954581369	28.50525558979813	23.702017232792482
37	15.615111833408852	27.19276237694206	32.45396912177166	24.73815666787743
38	16.141981434118833	29.31259806252785	22.996528726937754	31.548891776415566
39	23.119352628169363	24.428101209890322	27.449644072810607	25.00290208912971
40	21.072162786604707	26.198937273683853	32.06939550419586	20.65950443551558
41	21.66231665349805	28.23077412759456	24.889439765735876	25.217469453171514
42	18.139742144700037	20.40973753880374	36.28959479346487	25.160925523031352
43	22.156982426446085	26.14052102797614	25.421551850035012	26.280944695542765
44	18.471142000157275	21.810978509561917	30.39929001793678	29.318589472344026
45	17.59939187190366	17.89896236271246	27.98662417758539	36.51502158779849
46	25.51404423907223	23.33541784466521	28.680129863807764	22.470408052454793
47	12.748222236368607	21.493059326191073	42.12260671262577	23.636111724814548
48	18.84335833498721	23.51740691783156	27.701657748203512	29.937576998977715
49	17.334646450651377	17.41852618807784	41.10369258076233	24.143134780508447
50	22.8415009979442	18.179435234732203	30.695864803837498	28.2831989634861
51	17.619987343146764	17.024965455777778	27.03548786926744	38.31955933180802
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	67.0
1	124.0
2	181.0
3	535.5
4	890.0
5	624.0
6	358.0
7	402.5
8	447.0
9	493.0
10	539.0
11	640.0
12	741.0
13	841.0
14	941.0
15	884.5
16	828.0
17	741.5
18	655.0
19	581.0
20	507.0
21	626.5
22	746.0
23	725.5
24	705.0
25	882.0
26	1321.5
27	1584.0
28	1704.0
29	1824.0
30	2309.0
31	2794.0
32	3116.5
33	3439.0
34	3691.5
35	3944.0
36	4318.0
37	4692.0
38	5567.0
39	6442.0
40	8226.5
41	10011.0
42	12795.0
43	15579.0
44	39648.5
45	63718.0
46	53238.0
47	42758.0
48	41155.0
49	39552.0
50	29734.0
51	19916.0
52	16048.0
53	12180.0
54	10165.0
55	8150.0
56	6923.0
57	5696.0
58	5124.0
59	4552.0
60	4095.5
61	3639.0
62	3494.5
63	3350.0
64	2600.5
65	1851.0
66	1456.5
67	1062.0
68	830.5
69	599.0
70	504.5
71	410.0
72	304.0
73	198.0
74	217.5
75	177.5
76	118.0
77	89.5
78	61.0
79	36.0
80	11.0
81	12.0
82	13.0
83	8.5
84	4.0
85	2.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	267049.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.59269647143408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.02451061029775	20.426213915798225
2	12.903184987409366	7.636800736943407
3	5.43105520897921	4.821587049567682
4	2.8015741455451932	3.3162453332534483
5	1.750034798233515	2.58941242992859
6	1.1869361104432663	2.107478402839928
7	0.8554038493173219	1.7719594531340692
8	0.7022916218507599	1.662616223988856
9	0.6390221063687095	1.7019348509075112
>10	4.446581548078505	22.92463180914364
>50	0.145519885608716	2.9679946376882147
>100	0.0873119313652296	4.567701058607222
>500	0.011388512786769079	2.3508794266220807
>1k	0.01012312247712807	8.366629345176353
>5k	0.003796170928923027	8.589809360828912
>10k+	0.0012653903096410087	4.1981059655718616
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC	11211	4.1981059655718616	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG	9069	3.396005976431292	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	8647	3.237982542529648	No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	5223	1.9558208418679717	TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	4366	1.6349059535890418	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACCAGTAT	3514	1.3158633808776667	No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	3011	1.1275084347816318	TruSeq Adapter, Index 15 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	2710	1.0147950376148196	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTC	2416	0.9047028822425847	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTA	2283	0.8548992881456212	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT	2023	0.7575388786327603	No Hit
TCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	2020	0.7564154892922272	TruSeq Adapter, Index 21 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTT	930	0.34825069556523336	TruSeq Adapter, Index 21 (95% over 23bp)
AGGCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	806	0.30181726948986887	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTACCAGTATCGT	802	0.3003194170358249	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATG	755	0.28271965070080773	No Hit
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG	706	0.26437095813876854	TruSeq Adapter, Index 15 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG	589	0.22055877385798112	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTAT	579	0.21681414272287108	No Hit
TGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG	559	0.20932488045265102	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	552	0.206703638658074	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT	447	0.1673850117394186	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTACCAGTA	430	0.16101913880973157	No Hit
TTCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	395	0.14791292983684642	No Hit
ACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	375	0.14042366756662636	TruSeq Adapter, Index 15 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG	343	0.12844084793427424	TruSeq Adapter, Index 15 (95% over 22bp)
TGCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	341	0.12769192170725224	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCC	316	0.11833034386947713	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	280	0.10484967178308101	No Hit
CGTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	276	0.103351819329037	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTACCAGTATCG	274	0.10260289310201498	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2772936801860333	0.0
2	0.0	0.0	0.0	6.277874098011975	0.0
3	0.0	0.0	0.0	7.63043486401372	0.0
4	0.0	0.0	0.0	10.39509603106546	0.0
5	0.0	0.0	0.0	16.468138805986918	0.0
6	0.0	0.0	0.0	17.960748776441775	0.0
7	0.0	0.0	0.0	19.922561028125923	0.0
8	0.0	0.0	0.0	22.64116323221581	0.0
9	0.0	0.0	0.0	23.33953693891383	0.0
10	0.0	0.0	0.0	28.348355545237016	0.0
11	0.0	0.0	0.0	31.179671146493714	0.0
12	0.0	0.0	0.0	38.10761320956079	0.0
13	0.0	0.0	0.0	39.27556366060161	0.0
14	0.0	0.0	0.0	39.77322513845774	0.0
15	0.0	0.0	0.0	41.17633842478347	0.0
16	0.0	0.0	0.0	42.43902804354257	0.0
17	0.0	0.0	0.0	43.86011555931683	0.0
18	0.0	0.0	0.0	45.4178821115226	0.0
19	0.0	0.0	0.0	47.9020704065546	0.0
20	0.0	0.0	0.0	49.1340540500058	0.0
21	0.0	0.0	0.0	50.56937116409348	0.0
22	0.0	0.0	0.0	51.854154106549736	0.0
23	0.0	0.0	0.0	53.096622717179244	0.0
24	0.0	0.0	0.0	54.010687177259605	0.0
25	0.0	0.0	0.0	54.81728072376231	0.0
26	0.0	0.0	0.0	55.62200195469745	0.0
27	0.0	0.0	0.0	56.29715894835779	0.0
28	0.0	0.0	0.0	56.982426446082926	0.0
29	0.0	0.0	0.0	57.72274002149418	0.0
30	0.0	0.0	0.0	58.51173380166187	0.0
31	0.0	0.0	0.0	59.26178341802441	0.0
32	0.0	0.0	0.0	59.95042108377114	0.0
33	0.0	0.0	0.0	60.62932270856659	0.0
34	0.0	0.0	0.0	61.20974053450865	0.0
35	0.0	0.0	0.0	61.872540245423124	0.0
36	0.0	0.0	0.0	62.50575737037023	0.0
37	0.0	0.0	0.0	63.16968047062524	0.0
38	0.0	0.0	0.0	63.752345075248364	0.0
39	0.0	0.0	0.0	64.48704170395695	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCA	25	3.882736E-5	45.000004	32
GTTTGCG	25	3.882736E-5	45.000004	1
AAGTACG	25	3.882736E-5	45.000004	15
CAACGGA	25	3.882736E-5	45.000004	37
GGCACGT	50	2.1827873E-11	45.000004	11
ATAATCC	35	1.2072451E-7	45.000004	18
GATCCGG	50	2.1827873E-11	45.000004	5
CCATGGG	25	3.882736E-5	45.000004	3
ATACTCG	25	3.882736E-5	45.000004	10
ATCCGGC	50	2.1827873E-11	45.000004	6
GACCAAG	25	3.882736E-5	45.000004	42
TCTCGCG	25	3.882736E-5	45.000004	1
GCGATTT	25	3.882736E-5	45.000004	9
CTCGCGG	25	3.882736E-5	45.000004	2
GCGATCC	25	3.882736E-5	45.000004	9
ACTCGAC	25	3.882736E-5	45.000004	11
TCGTAGG	25	3.882736E-5	45.000004	2
CTCGACA	25	3.882736E-5	45.000004	12
CCGAAGG	25	3.882736E-5	45.000004	15
AACTCGA	25	3.882736E-5	45.000004	10
>>END_MODULE