FastQCFastQC Report
Sat 14 Jan 2017
SRR2933767.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933767.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681448
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGC138722.035665230509151No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCG123731.8156924666298824No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC106501.5628485225578475No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC83861.2306148084666768Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC79291.1635517310198285Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT64110.9407907866777803Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC52950.7770218710745354Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGCTAGTT49890.7321174909897747No Hit
GAACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT33350.489399044387833No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTA30800.4519787276505324No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTC24790.3637841772226201No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT24010.3523379626912105No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG23180.3401580164590695No Hit
TGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCTT18630.2733884316925136Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14090.20676559326610394No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT13800.20250994940186193No Hit
TCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG13790.20236320306171565No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGT11240.16494288632441506No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCC11170.16391566194339113No Hit
TGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG11070.16244819854192835No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC9850.1445451450440826No Hit
TTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG8880.13031075004989376No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATG8210.12047874526009321No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT7900.11592960871555863No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTAT7390.10844554536809851No Hit
CGTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT7250.10639109660605064No Hit
TTTCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC7030.10316267712283254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGG600.045.0000042
TCGTGCG302.1641954E-645.0000041
TGTTACG302.1641954E-645.0000041
CCCCACG302.1641954E-645.00000438
GGCGATA302.1641954E-645.0000048
CCACGAG302.1641954E-645.00000440
TAGTAGT302.1641954E-645.00000438
TCGTTGA207.0312625E-445.024
ACCGGGT207.0312625E-445.04
CTCGTCT207.0312625E-445.02
CCGTGGT253.889128E-545.028
TTACTAG253.889128E-545.016
CGCGAGG207.0312625E-445.02
ACGGGTA351.2109558E-745.05
TCTACGG406.8084773E-945.02
CTACGGG453.8380676E-1045.03
CGGGCCC551.8189894E-1245.06
TTACGGG1100.045.03
CGATGCG207.0312625E-445.010
TTTTACG207.0312625E-445.01
Produced by FastQC (version 0.11.5)