SRR2933766.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933766.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	657633
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGC	13636	2.0734969200146587	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCG	12669	1.9264544206267022	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC	10775	1.6384518416806944	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	7010	1.065944075190874	No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	5343	0.8124592287795777	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTCGCATT	5174	0.7867610049982284	No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	3869	0.5883220580475736	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT	3411	0.5186783509951599	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTA	3257	0.4952610346500252	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTC	2925	0.44477695006181256	No Hit
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	2743	0.41710193983574423	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCT	2477	0.3766538479668752	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1973	0.3000153581100705	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	1837	0.27933513068839305	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGT	1201	0.1826246553929015	No Hit
TGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTT	1193	0.1814081714269205	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCC	1126	0.1712201182118294	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATG	940	0.14293686600277053	No Hit
TCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	828	0.12590609047903617	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC	826	0.12560196948754093	No Hit
TGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	781	0.11875924717889765	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT	768	0.11678246073417847	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTAT	711	0.10811501247656367	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	100	0.0	45.000004	6
AGACGAA	25	3.8889782E-5	45.000004	11
TATCCGT	25	3.8889782E-5	45.000004	40
ACCGACG	20	7.031082E-4	45.0	17
ACGGGCT	40	6.8066583E-9	45.0	5
ATTCCGA	20	7.031082E-4	45.0	12
TACGCGG	20	7.031082E-4	45.0	2
CGAGGTA	55	1.8189894E-12	45.0	39
GAAACGC	20	7.031082E-4	45.0	21
GACGAAT	20	7.031082E-4	45.0	20
GCTAACC	40	6.8066583E-9	45.0	27
CTCGAAC	30	2.1640808E-6	44.999996	13
TTTGTCG	145	0.0	43.448273	1
TACGGCT	1745	0.0	42.421207	7
TCGTTCA	70	0.0	41.785713	16
ACGGCTG	1775	0.0	41.704224	8
CCGATGA	175	0.0	41.142857	18
TTGGGAC	905	0.0	41.022102	5
AATGATC	450	0.0	41.0	2
TCTTGCG	220	0.0	40.909092	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content