##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933766.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	657633
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.746007271532907	31.0	25.0	33.0	16.0	34.0
2	28.677152758453424	31.0	28.0	34.0	16.0	34.0
3	29.466380184692678	31.0	30.0	34.0	16.0	34.0
4	32.834652458133945	35.0	32.0	37.0	28.0	37.0
5	33.89384656791858	35.0	33.0	37.0	28.0	37.0
6	33.22730763206834	35.0	33.0	37.0	28.0	37.0
7	33.60348857189344	35.0	32.0	36.0	29.0	37.0
8	33.70638334755099	35.0	35.0	37.0	28.0	37.0
9	34.77469196345074	37.0	33.0	39.0	28.0	39.0
10	33.6894118756206	35.0	32.0	39.0	27.0	39.0
11	34.23679164518812	37.0	32.0	39.0	27.0	39.0
12	34.43028254360715	37.0	33.0	39.0	27.0	39.0
13	34.824963163344904	37.0	34.0	39.0	30.0	39.0
14	34.641158518505	37.0	33.0	39.0	27.0	40.0
15	35.022707193829994	37.0	33.0	39.0	27.0	40.0
16	34.55926937973003	36.0	32.0	39.0	27.0	40.0
17	34.48329235302973	36.0	32.0	39.0	27.0	40.0
18	34.401578083824866	37.0	32.0	39.0	27.0	40.0
19	34.175724150095874	36.0	32.0	39.0	25.0	40.0
20	35.20072289559678	37.0	33.0	39.0	28.0	40.0
21	34.55955525346204	37.0	32.0	39.0	25.0	40.0
22	35.43762706555176	37.0	34.0	39.0	28.0	40.0
23	36.24429887186318	38.0	34.0	39.0	30.0	40.0
24	36.34694883012258	38.0	35.0	40.0	31.0	40.0
25	34.336303074815284	37.0	32.0	39.0	25.0	40.0
26	34.943590117892505	37.0	33.0	39.0	27.0	40.0
27	35.6097549849232	37.0	34.0	39.0	30.0	40.0
28	35.80570470155847	38.0	34.0	40.0	30.0	40.0
29	36.27970007587819	38.0	35.0	40.0	30.0	41.0
30	34.31191865371719	37.0	33.0	39.0	24.0	40.0
31	34.50257362389053	37.0	32.0	39.0	25.0	40.0
32	35.293590802164736	37.0	34.0	39.0	30.0	40.0
33	36.10256784559169	38.0	35.0	40.0	30.0	40.0
34	36.33719262871541	38.0	35.0	40.0	30.0	41.0
35	36.53369280434528	38.0	35.0	40.0	31.0	41.0
36	36.34419957635946	38.0	35.0	40.0	30.0	41.0
37	36.626399526787736	39.0	35.0	40.0	31.0	41.0
38	35.93477669155897	38.0	35.0	40.0	30.0	41.0
39	36.00373916759043	38.0	35.0	40.0	30.0	41.0
40	35.48427922564713	38.0	34.0	40.0	29.0	41.0
41	35.03461657185695	37.0	33.0	40.0	27.0	41.0
42	35.905736178081085	38.0	35.0	40.0	30.0	41.0
43	36.01428456297053	38.0	35.0	40.0	30.0	41.0
44	36.43734271242471	38.0	35.0	40.0	31.0	41.0
45	36.673451605986926	39.0	35.0	40.0	32.0	41.0
46	36.4730769289254	38.0	35.0	40.0	31.0	41.0
47	36.31666598239443	38.0	35.0	40.0	31.0	41.0
48	36.36726107114455	38.0	35.0	40.0	31.0	41.0
49	36.515915411787425	39.0	35.0	40.0	31.0	41.0
50	36.35212344879287	38.0	35.0	40.0	31.0	41.0
51	35.658767123912575	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	7.0
10	14.0
11	14.0
12	22.0
13	29.0
14	31.0
15	52.0
16	92.0
17	152.0
18	348.0
19	662.0
20	1016.0
21	1377.0
22	1785.0
23	2407.0
24	3395.0
25	4791.0
26	6464.0
27	8518.0
28	10928.0
29	14807.0
30	20382.0
31	28413.0
32	39676.0
33	52202.0
34	66026.0
35	87638.0
36	111680.0
37	117206.0
38	69971.0
39	7525.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.999759744416718	3.8592953820748046	50.81633677142114	19.324608102087335
2	18.236919376004547	13.969037441855866	47.422650627325574	20.37139255481401
3	17.35268759323209	13.605764917514785	48.867225336928044	20.17432215232508
4	18.181265234560918	3.4531417979328896	54.91360682934099	23.451986138165207
5	24.586357436442512	4.504488065531991	46.362636911468854	24.546517586556636
6	20.07320192265291	12.904309850630973	48.97093059502793	18.05155763168819
7	72.8126477837943	1.5376357330000168	20.391312479756944	5.2584040034487325
8	71.86135732239714	8.946175146320211	11.143753430864935	8.04871410041771
9	66.72338523158054	4.13011512500133	13.435000980790198	15.71149866262794
10	36.598832479513646	22.961591039379105	21.9619453403342	18.477631140773045
11	27.501357139924547	23.529536990996498	28.282339846084366	20.686766022994586
12	23.336572221892755	19.431810751589413	33.098703988394746	24.132913038123085
13	23.344631428167382	20.861787653600107	36.89443808324704	18.899142834985472
14	19.477733021305195	25.39440691084541	30.329682360830436	24.798177707018958
15	15.360999219929658	24.426237734420262	37.946240532333384	22.266522513316698
16	17.02149983349376	23.73542690223879	30.95647572430216	28.286597539965303
17	16.901524102348876	25.28598777737735	33.276918889410965	24.535569230862805
18	16.550264357171855	23.3242553217372	34.59132981465346	25.53415050643748
19	18.139600658726067	25.55711164129537	31.488687459418855	24.814600240559702
20	20.670495549949592	25.472413945163947	35.19531410376304	18.661776401123422
21	22.43242051417736	27.152074181192244	29.713381171565295	20.702124133065098
22	18.844401056516325	23.17858136681097	31.60775082758925	26.369266749083454
23	19.203415886976476	27.473530069202734	29.23210970252405	24.09094434129674
24	21.783274257830733	22.969346124662234	28.5110388316888	26.73634078581823
25	18.125763153613033	27.880291895327638	25.59421440225779	28.399730548801532
26	18.494966037288275	24.11405753665038	29.382345472322708	28.008630953738635
27	22.099255967994306	24.780386629016487	28.74627641861038	24.374080984378825
28	16.361253160957556	23.692393781942208	33.91648533452549	26.02986772257475
29	21.105540628283556	20.33155270492813	28.256793682798765	30.306112983989554
30	24.375297468344804	23.62366243786428	29.031389848137184	22.96965024565373
31	20.928542211233317	23.54748012949472	26.885664192642402	28.638313466629562
32	21.242243013960675	28.195817424003966	24.675616947446372	25.886322614588984
33	22.318679263358135	21.706939888965426	27.154203028132713	28.82017781954373
34	20.12292570476238	22.882063400103096	27.756666712284815	29.23834418284971
35	20.73344859518911	23.454114985105676	26.348890642653274	29.46354577705194
36	20.64875089905768	22.304537637253606	30.245136725194747	26.80157473849396
37	20.46475769920305	22.669939008535156	31.92160369081235	24.94369960144944
38	21.221866907530494	24.28527765486221	29.174934956122943	25.31792048148435
39	21.418937310019416	21.089422215734306	28.97695219065953	28.514688283586743
40	23.634762854053857	22.557718362673405	28.714343714503375	25.09317506876936
41	18.62345715619502	24.662539744812076	28.341947560417434	28.37205553857547
42	19.360342318588028	24.128047102259163	31.27671512834666	25.23489545080615
43	22.26059215398254	20.215834667664183	31.097891985347452	26.425681193005822
44	21.594111001120687	19.9936742833769	30.26003865377802	28.152176061724397
45	20.940859111388875	18.810947747451845	28.6294939578762	31.618699183283077
46	24.16332513727261	21.413463132172502	28.69320730559446	25.730004424960427
47	17.727516715249994	20.976289206898073	36.481289716300736	24.8149043615512
48	19.444583833232212	21.32709277058785	30.21715759397719	29.011165802202747
49	19.659749434715106	19.359429955613543	35.50703812004568	25.473782489625673
50	21.839080459770116	17.808260838491986	31.122221664667073	29.23043703707083
51	19.246296946777306	18.29014054951622	28.215129106963914	34.24843339674256
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	144.0
1	488.0
2	832.0
3	2230.5
4	3629.0
5	2625.5
6	1622.0
7	1607.5
8	1593.0
9	1649.5
10	1706.0
11	1689.5
12	1673.0
13	1690.5
14	1708.0
15	1591.0
16	1474.0
17	1528.5
18	1583.0
19	1549.0
20	1515.0
21	1716.5
22	1918.0
23	2084.5
24	2251.0
25	2866.0
26	4198.0
27	4915.0
28	6119.5
29	7324.0
30	8113.0
31	8902.0
32	10416.0
33	11930.0
34	12931.5
35	13933.0
36	15400.0
37	16867.0
38	18771.0
39	20675.0
40	21408.0
41	22141.0
42	24703.0
43	27265.0
44	29789.5
45	32314.0
46	36303.5
47	40293.0
48	73875.0
49	107457.0
50	97021.0
51	86585.0
52	82577.5
53	78570.0
54	62212.0
55	45854.0
56	38618.5
57	31383.0
58	27543.0
59	23703.0
60	20658.0
61	17613.0
62	14556.5
63	11500.0
64	10006.5
65	8513.0
66	6885.5
67	5258.0
68	4377.0
69	3496.0
70	2959.5
71	2423.0
72	1998.0
73	1573.0
74	1212.0
75	685.0
76	519.0
77	419.0
78	319.0
79	261.0
80	203.0
81	132.0
82	61.0
83	52.5
84	44.0
85	23.5
86	3.0
87	5.0
88	7.0
89	6.5
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	657633.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.118393552844985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.64219338558597	25.87600935689622
2	11.82271784125354	8.540351517092743
3	4.524919975335607	4.902985213929031
4	2.3160398256772035	3.3460655163148694
5	1.5429234758172923	2.786395866074623
6	1.1235121632126877	2.434767268339443
7	0.9198311804084336	2.325597720331883
8	0.7977759595549502	2.3051508859363383
9	0.6838263511559646	2.2228838345571393
>10	4.473472150149511	25.838244738987537
>50	0.08596967621560403	2.1889323349335226
>100	0.05490142685788894	3.65677747842244
>500	0.004681517018889755	1.1833146844716442
>1k	0.004681517018889755	3.9984907212881797
>5k	0.001276777368788115	2.6942006332468833
>10k+	0.001276777368788115	5.699832229177522
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGC	13636	2.0734969200146587	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCG	12669	1.9264544206267022	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC	10775	1.6384518416806944	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	7010	1.065944075190874	No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	5343	0.8124592287795777	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTCGCATT	5174	0.7867610049982284	No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	3869	0.5883220580475736	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT	3411	0.5186783509951599	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTA	3257	0.4952610346500252	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTC	2925	0.44477695006181256	No Hit
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	2743	0.41710193983574423	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCT	2477	0.3766538479668752	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1973	0.3000153581100705	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	1837	0.27933513068839305	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGT	1201	0.1826246553929015	No Hit
TGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTT	1193	0.1814081714269205	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCC	1126	0.1712201182118294	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATG	940	0.14293686600277053	No Hit
TCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	828	0.12590609047903617	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC	826	0.12560196948754093	No Hit
TGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	781	0.11875924717889765	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT	768	0.11678246073417847	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTAT	711	0.10811501247656367	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6454968044486818	0.0
2	0.0	0.0	0.0	3.138072450743804	0.0
3	0.0	0.0	0.0	4.095901513458114	0.0
4	0.0	0.0	0.0	5.772672600067211	0.0
5	0.0	0.0	0.0	9.177155039360859	0.0
6	0.0	0.0	0.0	10.24264293306449	0.0
7	0.0	0.0	0.0	11.623504294948702	0.0
8	0.0	0.0	0.0	13.480771190010234	0.0
9	0.0	0.0	0.0	14.094335290351914	0.0
10	0.0	0.0	0.0	17.352535532736344	0.0
11	0.0	0.0	0.0	19.901069441466593	0.0
12	0.0	0.0	0.0	24.17625027941116	0.0
13	0.0	0.0	0.0	25.017296881391292	0.0
14	0.0	0.0	0.0	25.35912887583196	0.0
15	0.0	0.0	0.0	26.29141177526067	0.0
16	0.0	0.0	0.0	27.290753353314084	0.0
17	0.0	0.0	0.0	28.56502030767921	0.0
18	0.0	0.0	0.0	29.785914028036913	0.0
19	0.0	0.0	0.0	31.589959749586775	0.0
20	0.0	0.0	0.0	32.60891713159163	0.0
21	0.0	0.0	0.0	33.84471278053261	0.0
22	0.0	0.0	0.0	35.2363704376149	0.0
23	0.0	0.0	0.0	36.4482925887235	0.0
24	0.0	0.0	0.0	37.43820641604056	0.0
25	0.0	0.0	0.0	38.294459067595454	0.0
26	0.0	0.0	0.0	39.06297281310397	0.0
27	0.0	0.0	0.0	39.84228285381056	0.0
28	0.0	0.0	0.0	40.60395387701043	0.0
29	0.0	0.0	0.0	41.406833294557906	0.0
30	0.0	0.0	0.0	42.24833607802528	0.0
31	0.0	0.0	0.0	43.0258213927829	0.0
32	0.0	0.0	0.0	43.782930601110344	0.0
33	0.0	0.0	0.0	44.51935958201611	0.0
34	0.0	0.0	0.0	45.276164669352056	0.0
35	0.0015206049574762823	0.0	0.0	46.023389945455904	0.0
36	0.0015206049574762823	0.0	0.0	46.75829832140419	0.0
37	0.0015206049574762823	0.0	0.0	47.501722085114345	0.0
38	0.0015206049574762823	0.0	0.0	48.21275696323025	0.0
39	0.0015206049574762823	0.0	0.0	48.94386382678485	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	100	0.0	45.000004	6
AGACGAA	25	3.8889782E-5	45.000004	11
TATCCGT	25	3.8889782E-5	45.000004	40
ACCGACG	20	7.031082E-4	45.0	17
ACGGGCT	40	6.8066583E-9	45.0	5
ATTCCGA	20	7.031082E-4	45.0	12
TACGCGG	20	7.031082E-4	45.0	2
CGAGGTA	55	1.8189894E-12	45.0	39
GAAACGC	20	7.031082E-4	45.0	21
GACGAAT	20	7.031082E-4	45.0	20
GCTAACC	40	6.8066583E-9	45.0	27
CTCGAAC	30	2.1640808E-6	44.999996	13
TTTGTCG	145	0.0	43.448273	1
TACGGCT	1745	0.0	42.421207	7
TCGTTCA	70	0.0	41.785713	16
ACGGCTG	1775	0.0	41.704224	8
CCGATGA	175	0.0	41.142857	18
TTGGGAC	905	0.0	41.022102	5
AATGATC	450	0.0	41.0	2
TCTTGCG	220	0.0	40.909092	1
>>END_MODULE