SRR2933765.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933765.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	345332
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGC	5905	1.7099486870605678	No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	5509	1.5952764296387245	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCG	5247	1.5194074108394242	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC	4643	1.34450326063035	No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	4145	1.200294209630153	No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT	3743	1.083884493762524	No Hit
TCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	2533	0.733497040529114	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGGTGGCTT	2242	0.649230305908517	No Hit
GAACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT	1574	0.45579326561106415	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTA	1403	0.40627569990617723	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTC	1178	0.3411210081892208	No Hit
CGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	1116	0.3231672709161039	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCT	1076	0.3115842146108672	No Hit
TGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCTT	1038	0.30058031112089234	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	859	0.24874613415495814	No Hit
TCCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	716	0.20733670786373692	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT	525	0.1520276140062317	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGT	511	0.14797354429939882	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC	510	0.1476839678917679	No Hit
TGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	490	0.14189243973914958	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCC	470	0.13610091158653123	No Hit
TTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	467	0.13523218236363846	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATG	417	0.12075336198209259	No Hit
TGCCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT	365	0.10569538878528488	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTT	30	2.1610758E-6	45.000004	33
CCTCGAT	25	3.8851183E-5	45.000004	15
ATCCGGC	50	2.1827873E-11	45.000004	6
TCTCGGA	25	3.8851183E-5	45.000004	21
TAGGGTA	30	2.1610758E-6	45.000004	5
CGGGATA	30	2.1610758E-6	45.000004	6
TAGGGCA	25	3.8851183E-5	45.000004	5
CTCGATC	25	3.8851183E-5	45.000004	16
CCAAACG	25	3.8851183E-5	45.000004	2
GTACGGT	30	2.1610758E-6	45.000004	32
CCTTCCG	20	7.026425E-4	45.0	1
CGGGTTG	20	7.026425E-4	45.0	6
ATGGGCG	40	6.7921064E-9	45.0	5
CGGGTAC	20	7.026425E-4	45.0	6
AGCCGGG	20	7.026425E-4	45.0	11
TCCGCGG	70	0.0	45.0	2
AGTTTCG	20	7.026425E-4	45.0	12
CTCGTAC	35	1.2086275E-7	45.0	29
TTAATAG	40	6.7921064E-9	45.0	22
GTACAGC	20	7.026425E-4	45.0	11

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content