##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933765.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	345332
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.663581712670705	31.0	25.0	33.0	16.0	34.0
2	28.887563272445067	31.0	28.0	33.0	16.0	34.0
3	29.659096174116502	31.0	30.0	34.0	16.0	34.0
4	32.97325472299121	35.0	32.0	37.0	28.0	37.0
5	33.98305109286136	35.0	33.0	37.0	28.0	37.0
6	33.54183510361044	35.0	33.0	37.0	28.0	37.0
7	33.81028112077653	35.0	33.0	36.0	30.0	37.0
8	33.97727983505728	35.0	35.0	37.0	29.0	37.0
9	35.04045671991012	37.0	34.0	39.0	30.0	39.0
10	33.85400426256472	35.0	32.0	39.0	27.0	39.0
11	34.32193946694775	37.0	32.0	39.0	27.0	39.0
12	34.33834686620412	37.0	32.0	39.0	27.0	39.0
13	34.82957559681697	37.0	34.0	39.0	28.0	39.0
14	34.40188282580242	37.0	32.0	39.0	25.0	40.0
15	34.89647933003602	37.0	32.0	39.0	27.0	40.0
16	34.47978177521921	36.0	32.0	39.0	27.0	40.0
17	34.42681245873536	36.0	32.0	38.0	27.0	40.0
18	34.396511183440865	37.0	32.0	39.0	27.0	40.0
19	33.983737388947446	36.0	32.0	39.0	25.0	40.0
20	35.294334148008296	37.0	34.0	39.0	29.0	40.0
21	34.409353897119296	37.0	32.0	39.0	25.0	40.0
22	35.402586496472956	37.0	34.0	39.0	28.0	40.0
23	36.34548202888814	38.0	34.0	39.0	31.0	40.0
24	36.435160946567365	38.0	35.0	40.0	31.0	40.0
25	34.3569405673381	37.0	32.0	39.0	25.0	40.0
26	34.91356723385032	37.0	33.0	39.0	27.0	40.0
27	35.662365491758656	37.0	34.0	39.0	30.0	40.0
28	35.96135602840165	38.0	34.0	40.0	30.0	40.0
29	36.31494330093938	38.0	35.0	40.0	30.0	41.0
30	34.34227641805567	37.0	33.0	39.0	24.0	40.0
31	35.06799833203989	37.0	33.0	39.0	27.0	40.0
32	35.48600187645512	37.0	34.0	39.0	30.0	40.0
33	36.3093313101595	38.0	35.0	40.0	31.0	40.0
34	36.54660153128005	38.0	35.0	40.0	31.0	41.0
35	36.77567963582871	39.0	35.0	40.0	31.0	41.0
36	36.56919138683933	38.0	35.0	40.0	31.0	41.0
37	36.78248178564396	39.0	35.0	40.0	31.0	41.0
38	36.21024405499635	38.0	35.0	40.0	30.0	41.0
39	36.082833331402824	38.0	35.0	40.0	30.0	41.0
40	35.76051741512516	38.0	34.0	40.0	30.0	41.0
41	35.24251155409866	37.0	33.0	40.0	29.0	41.0
42	36.244237429488145	38.0	35.0	40.0	31.0	41.0
43	36.2486187205356	38.0	35.0	40.0	31.0	41.0
44	36.60315290792629	38.0	35.0	40.0	31.0	41.0
45	36.894585500330116	39.0	35.0	40.0	32.0	41.0
46	36.65776701840548	39.0	35.0	40.0	32.0	41.0
47	36.62766844659632	39.0	35.0	40.0	31.0	41.0
48	36.63112019737528	38.0	35.0	40.0	32.0	41.0
49	36.80879269804131	39.0	35.0	40.0	32.0	41.0
50	36.65251410237105	39.0	35.0	40.0	32.0	41.0
51	35.85465580948189	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	9.0
11	7.0
12	5.0
13	12.0
14	9.0
15	31.0
16	22.0
17	66.0
18	116.0
19	243.0
20	334.0
21	480.0
22	770.0
23	989.0
24	1493.0
25	2242.0
26	3097.0
27	4020.0
28	5359.0
29	7428.0
30	10263.0
31	14752.0
32	20602.0
33	27422.0
34	34301.0
35	46006.0
36	59541.0
37	64675.0
38	37664.0
39	3370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.144313298506944	3.6153614492720045	50.09121656840374	19.14910868381731
2	17.671979428492	11.50168533469241	49.92123521712439	20.905100019691194
3	18.16628635631798	11.478229645674308	50.955602145182034	19.399881852825686
4	18.515225927513235	3.614203143641481	54.323086189521966	23.54748473932332
5	22.639952277808025	4.7021417071108385	48.58310263746192	24.07480337761922
6	19.896794968320343	10.437492036648791	50.382530434480444	19.283182560550426
7	72.51311781126569	1.4351406762188272	19.602585338167327	6.449156174348164
8	71.52450395561372	7.084197236282766	11.78199529727914	9.609303510824367
9	67.21415912802752	3.5927744894767932	14.77042382403021	14.422642558465476
10	36.259889034320594	22.683678315360293	23.460611817034042	17.59582083328507
11	26.74238124471523	25.19720153359665	28.02578388333546	20.03463333835266
12	22.138695516198904	20.26484658241924	34.39298993432407	23.20346796705779
13	21.27257248097483	23.261672824991603	36.11452167768988	19.35123301634369
14	18.55721450661972	26.263132290086062	31.03332445299017	24.146328750304054
15	15.022065722261477	25.430600118147172	37.983447812539815	21.563886347051533
16	16.64224572295646	26.279927721728658	29.972895648245746	27.104930907069143
17	16.730566527283887	26.17191572168232	31.74394495731644	25.353572793717348
18	17.313194259437296	23.5136042996305	34.705732454565464	24.467468986366743
19	18.50422202402326	24.335422144487044	31.451472785609212	25.708883045880487
20	20.023919011270312	26.163518005861025	35.22407422422481	18.588488758643855
21	21.854621060312972	27.86564812991556	30.14084996467168	20.138880845099788
22	18.493797273348545	25.165348128757252	30.83901868346982	25.50183591442438
23	19.056154656967788	26.153382831593948	29.470191004598473	25.32027150683979
24	21.82508426673462	23.149606755238437	28.304645963884028	26.720663014142907
25	16.971204522025182	27.11680353978201	27.361495604230136	28.550496333962677
26	17.825165348128756	23.970266294464455	32.82551283981792	25.37905551758887
27	22.95095733960363	24.82683330823671	28.77810339036058	23.44410596179908
28	18.66551608307368	22.96717361843096	34.166251607149064	24.2010586913463
29	21.362051590932786	19.640230271159346	34.90467144660791	24.093046691299964
30	27.552616033266535	22.85713458353121	29.504940173514182	20.085309209688067
31	27.88041652670474	22.681651280506873	26.702419700462166	22.735512492326226
32	23.16958752736497	24.184842412518968	29.095189556716434	23.550380503399627
33	24.90530851470469	20.345348823740633	29.180325020559927	25.56901764099475
34	23.819107409681116	20.393128930999733	32.13313564917239	23.65462801014676
35	20.414268008756792	19.819478067482883	30.40581237765397	29.360441546106358
36	26.41139541079309	20.705871451241126	32.394623145263104	20.488109992702675
37	24.46660025714385	21.062050432627153	34.27918640612512	20.192162904103878
38	25.201255603303487	23.15337124853764	28.121054521446027	23.524318626712844
39	25.513129394321986	20.207510453708316	34.45901335526392	19.820346796705778
40	27.04527816709717	19.60924559554284	28.990362897154043	24.355113340205946
41	23.670844288974088	21.296028169992933	27.764585963652372	27.268541577380606
42	21.587342036069636	18.00557145008282	32.51392862520704	27.893157888640495
43	25.484461329966525	17.97169101039	32.180336603616226	24.363511056027242
44	23.075475194884923	18.36551492476805	32.099834362294835	26.45917551805219
45	23.635226390835488	17.28568450071236	29.529264591755183	29.549824516696976
46	27.025297394970636	19.035015579210732	32.17193888779493	21.7677481380237
47	20.444094378742776	18.588199182236227	37.86472148541114	23.10298495360986
48	21.97595357511033	19.607508137097053	31.85572145066197	26.560816837130645
49	23.067077479063624	16.153730323283103	37.576013807003115	23.203178390650155
50	25.542376611492706	15.457299062930746	31.927825976162072	27.072498349414474
51	21.358866250448845	15.913092328541808	29.158027637172346	33.570013783837005
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	52.0
1	187.0
2	322.0
3	946.0
4	1570.0
5	1145.0
6	720.0
7	729.5
8	739.0
9	830.5
10	922.0
11	947.0
12	972.0
13	935.5
14	899.0
15	910.0
16	921.0
17	959.0
18	997.0
19	1020.0
20	1043.0
21	1008.5
22	974.0
23	1140.0
24	1306.0
25	1650.5
26	2274.0
27	2553.0
28	3023.0
29	3493.0
30	3492.0
31	3491.0
32	4522.5
33	5554.0
34	5868.5
35	6183.0
36	6936.0
37	7689.0
38	8546.0
39	9403.0
40	10514.0
41	11625.0
42	13570.0
43	15515.0
44	17097.0
45	18679.0
46	21101.5
47	23524.0
48	39807.5
49	56091.0
50	54180.5
51	52270.0
52	49759.0
53	47248.0
54	36035.0
55	24822.0
56	19960.5
57	15099.0
58	12160.0
59	9221.0
60	7680.5
61	6140.0
62	5139.0
63	4138.0
64	3789.0
65	3440.0
66	2762.0
67	2084.0
68	1673.0
69	1262.0
70	1096.5
71	931.0
72	757.5
73	584.0
74	497.5
75	320.0
76	229.0
77	172.0
78	115.0
79	88.5
80	62.0
81	41.0
82	20.0
83	18.5
84	17.0
85	11.5
86	6.0
87	3.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	345332.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.521460570575485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.99270225715132	24.8624301345543
2	12.883658114737633	9.152927074548554
3	5.220126800346189	5.562795849357046
4	2.630251596364068	3.7372151348375784
5	1.5341595018419296	2.7247793126825917
6	1.0981581966764524	2.3404909850098132
7	0.8074848469506963	2.0078128806607443
8	0.6531837664114116	1.856163312313836
9	0.5538879864174798	1.7707419243039566
>10	4.450784467949609	26.731855686657944
>50	0.10175096111682315	2.546362765571417
>100	0.05826063096205196	3.743468062984628
>500	0.004102861335355772	0.909706752672664
>1k	0.009026294937782698	7.196914267940002
>5k	0.002461716801213463	4.856335855904921
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGC	5905	1.7099486870605678	No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	5509	1.5952764296387245	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCG	5247	1.5194074108394242	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC	4643	1.34450326063035	No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	4145	1.200294209630153	No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT	3743	1.083884493762524	No Hit
TCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	2533	0.733497040529114	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGGTGGCTT	2242	0.649230305908517	No Hit
GAACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT	1574	0.45579326561106415	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTA	1403	0.40627569990617723	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTC	1178	0.3411210081892208	No Hit
CGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	1116	0.3231672709161039	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCT	1076	0.3115842146108672	No Hit
TGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCTT	1038	0.30058031112089234	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	859	0.24874613415495814	No Hit
TCCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	716	0.20733670786373692	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT	525	0.1520276140062317	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGT	511	0.14797354429939882	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC	510	0.1476839678917679	No Hit
TGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	490	0.14189243973914958	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCC	470	0.13610091158653123	No Hit
TTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	467	0.13523218236363846	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATG	417	0.12075336198209259	No Hit
TGCCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT	365	0.10569538878528488	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2223020166101028	0.0
2	0.0	0.0	0.0	5.055424924420557	0.0
3	2.895764076309175E-4	0.0	0.0	6.326086201105023	0.0
4	2.895764076309175E-4	0.0	0.0	8.23120938690883	0.0
5	2.895764076309175E-4	0.0	0.0	11.837014814729015	0.0
6	2.895764076309175E-4	0.0	0.0	13.011826300487646	0.0
7	2.895764076309175E-4	0.0	0.0	14.520808960652358	0.0
8	2.895764076309175E-4	0.0	0.0	16.49427217865706	0.0
9	2.895764076309175E-4	0.0	0.0	17.196784543569667	0.0
10	2.895764076309175E-4	0.0	0.0	20.17044467353156	0.0
11	2.895764076309175E-4	0.0	0.0	23.06562959702547	0.0
12	2.895764076309175E-4	0.0	0.0	27.245954617585397	0.0
13	2.895764076309175E-4	0.0	0.0	28.249047293618894	0.0
14	2.895764076309175E-4	0.0	0.0	28.62115297742462	0.0
15	2.895764076309175E-4	0.0	0.0	29.51275873652022	0.0
16	2.895764076309175E-4	0.0	0.0	30.604461793288777	0.0
17	2.895764076309175E-4	0.0	0.0	32.197421611666456	0.0
18	2.895764076309175E-4	0.0	0.0	33.82628890459036	0.0
19	2.895764076309175E-4	0.0	0.0	35.6202147498639	0.0
20	2.895764076309175E-4	0.0	0.0	36.93662909895405	0.0
21	2.895764076309175E-4	0.0	0.0	38.49686678326943	0.0
22	2.895764076309175E-4	0.0	0.0	40.02930513245225	0.0
23	2.895764076309175E-4	0.0	0.0	41.33616346009058	0.0
24	2.895764076309175E-4	0.0	0.0	42.454507546361185	0.0
25	2.895764076309175E-4	0.0	0.0	43.45412530550311	0.0
26	2.895764076309175E-4	0.0	0.0	44.32198579917297	0.0
27	2.895764076309175E-4	0.0	0.0	45.17420916683076	0.0
28	2.895764076309175E-4	0.0	0.0	46.09593087231997	0.0
29	2.895764076309175E-4	0.0	0.0	47.01417766091761	0.0
30	2.895764076309175E-4	0.0	0.0	47.972675570175944	0.0
31	2.895764076309175E-4	0.0	0.0	48.82721554909478	0.0
32	2.895764076309175E-4	0.0	0.0	49.61486337785088	0.0
33	2.895764076309175E-4	0.0	0.0	50.40917146398249	0.0
34	2.895764076309175E-4	0.0	0.0	51.17104699245943	0.0
35	2.895764076309175E-4	0.0	0.0	51.930026756860066	0.0
36	2.895764076309175E-4	0.0	0.0	52.68234626388519	0.0
37	2.895764076309175E-4	0.0	0.0	53.41178923470747	0.0
38	2.895764076309175E-4	0.0	0.0	54.1866957015278	0.0
39	2.895764076309175E-4	0.0	0.0	55.01546338016749	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTT	30	2.1610758E-6	45.000004	33
CCTCGAT	25	3.8851183E-5	45.000004	15
ATCCGGC	50	2.1827873E-11	45.000004	6
TCTCGGA	25	3.8851183E-5	45.000004	21
TAGGGTA	30	2.1610758E-6	45.000004	5
CGGGATA	30	2.1610758E-6	45.000004	6
TAGGGCA	25	3.8851183E-5	45.000004	5
CTCGATC	25	3.8851183E-5	45.000004	16
CCAAACG	25	3.8851183E-5	45.000004	2
GTACGGT	30	2.1610758E-6	45.000004	32
CCTTCCG	20	7.026425E-4	45.0	1
CGGGTTG	20	7.026425E-4	45.0	6
ATGGGCG	40	6.7921064E-9	45.0	5
CGGGTAC	20	7.026425E-4	45.0	6
AGCCGGG	20	7.026425E-4	45.0	11
TCCGCGG	70	0.0	45.0	2
AGTTTCG	20	7.026425E-4	45.0	12
CTCGTAC	35	1.2086275E-7	45.0	29
TTAATAG	40	6.7921064E-9	45.0	22
GTACAGC	20	7.026425E-4	45.0	11
>>END_MODULE