FastQCFastQC Report
Sat 14 Jan 2017
SRR2933764.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933764.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409648
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC116752.85000781158458No Hit
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC99862.4377026129750416No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG96582.3576338710307385No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC68751.6782701245947742No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC63821.5579228996601961No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT45831.1187653790571417No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTTTGCGT39700.9691247119478186No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC37830.9234757645588407No Hit
GAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT34720.8475569269226263No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTA29260.7142717650275359No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC23670.577813146896848No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT23090.5636546498457212No Hit
TGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT22190.5416845682146623Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG14860.36275045893059404No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10530.2570499550833887No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGT9960.24313557005038472No Hit
TCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG9360.22848884896301214No Hit
TGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG8440.20603054329570752No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATG7860.1918720462445807No Hit
TTCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT7810.19065148615396635No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTAT6780.16550794828731008No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCC6300.15379057141741204No Hit
TTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG6270.1530582353630434No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC5690.13889973831191657No Hit
CGTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT4990.12181189704331523No Hit
TGCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT4890.11937077686208647No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACTTTGCG4780.11668554466273484No Hit
TTTCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC4210.10277115962973091No Hit
GTTCAGGGGAAATGTAATACTGGAATAATTTGTAAATGATTTTAATTTTAT4140.10106237550287075No Hit
TTGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT4110.10033003944850212No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCG207.027965E-445.025
CGGGTTC207.027965E-445.06
GCAAGCC207.027965E-445.044
GGTCGCT207.027965E-445.035
GACACGA406.7957444E-945.025
TCGGGTA253.8863946E-545.05
CATTCTA207.027965E-445.045
AGGCGCG207.027965E-445.010
CCGCTAC207.027965E-445.034
CCGTCGC207.027965E-445.022
ATTCCGG253.8863946E-545.04
AGTGCGG207.027965E-445.021
ACTAGGG207.027965E-445.03
TATCTTG207.027965E-445.01
GCTTAGA207.027965E-445.014
AGACACG406.7957444E-945.024
ATTGGTG207.027965E-445.012
GGACCCA406.7957444E-945.08
ATACGAA406.7957444E-945.019
GCTAATT207.027965E-445.029
Produced by FastQC (version 0.11.5)