##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933764.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	409648
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.79682312619615	31.0	16.0	33.0	16.0	34.0
2	27.952190661250636	31.0	25.0	33.0	16.0	34.0
3	28.815426903097293	31.0	28.0	34.0	16.0	34.0
4	32.43449986329727	35.0	30.0	35.0	26.0	37.0
5	33.79865884857243	35.0	33.0	37.0	28.0	37.0
6	33.035608620083586	35.0	33.0	37.0	28.0	37.0
7	33.63221595125571	35.0	32.0	35.0	30.0	37.0
8	33.50846092254814	35.0	33.0	37.0	28.0	37.0
9	34.725129867593644	37.0	33.0	39.0	28.0	39.0
10	34.05172001327969	35.0	32.0	39.0	27.0	39.0
11	34.55904825606374	37.0	33.0	39.0	27.0	39.0
12	34.50971077608093	37.0	33.0	39.0	27.0	39.0
13	34.93841542006796	37.0	34.0	39.0	30.0	39.0
14	34.62273219935164	37.0	33.0	39.0	27.0	40.0
15	35.05971956411358	37.0	33.0	39.0	27.0	40.0
16	34.32425155255243	36.0	32.0	39.0	27.0	40.0
17	34.448367867046834	36.0	32.0	38.0	27.0	40.0
18	34.36352917626841	37.0	32.0	39.0	27.0	40.0
19	33.95038179119634	36.0	32.0	39.0	25.0	40.0
20	35.14170946764051	37.0	33.0	39.0	27.0	40.0
21	34.5305603835488	37.0	32.0	39.0	27.0	40.0
22	35.51534732257939	37.0	34.0	39.0	29.0	40.0
23	36.28166376987072	38.0	34.0	39.0	31.0	40.0
24	36.39628656798031	38.0	35.0	40.0	31.0	40.0
25	34.304510213646836	37.0	32.0	39.0	25.0	40.0
26	34.99606491426786	37.0	33.0	39.0	27.0	40.0
27	35.829233878842324	37.0	34.0	39.0	30.0	40.0
28	35.92692994961528	38.0	34.0	40.0	30.0	40.0
29	36.322747822520796	38.0	35.0	40.0	30.0	40.0
30	34.57481301019412	37.0	33.0	39.0	25.0	40.0
31	35.14848357614342	37.0	33.0	39.0	27.0	40.0
32	35.6913496465258	37.0	34.0	40.0	30.0	40.0
33	36.35813918290825	38.0	35.0	40.0	31.0	40.0
34	36.47639436784752	38.0	35.0	40.0	31.0	41.0
35	36.70888421278757	38.0	35.0	40.0	31.0	41.0
36	36.57787417490138	38.0	35.0	40.0	31.0	41.0
37	36.80856979650822	39.0	35.0	40.0	31.0	41.0
38	36.28990987384291	38.0	35.0	40.0	30.0	41.0
39	36.2905348006093	38.0	35.0	40.0	31.0	41.0
40	35.8494976174667	38.0	34.0	40.0	30.0	41.0
41	35.22294262391126	37.0	33.0	40.0	29.0	40.0
42	36.21207231574425	38.0	35.0	40.0	31.0	41.0
43	36.23936648049057	38.0	35.0	40.0	31.0	41.0
44	36.645412646955435	38.0	35.0	40.0	31.0	41.0
45	36.977956684763505	39.0	35.0	40.0	32.0	41.0
46	36.794626118033044	39.0	35.0	40.0	32.0	41.0
47	36.63378070929188	39.0	35.0	40.0	31.0	41.0
48	36.69780885052533	39.0	35.0	40.0	31.0	41.0
49	36.891682615318516	39.0	35.0	40.0	32.0	41.0
50	36.70959701988048	39.0	35.0	40.0	31.0	41.0
51	35.97545209545756	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	15.0
11	12.0
12	9.0
13	10.0
14	14.0
15	30.0
16	39.0
17	71.0
18	142.0
19	253.0
20	420.0
21	591.0
22	812.0
23	1124.0
24	1657.0
25	2409.0
26	3506.0
27	4696.0
28	6259.0
29	8844.0
30	12387.0
31	17624.0
32	24434.0
33	33362.0
34	43506.0
35	58084.0
36	72855.0
37	73163.0
38	39820.0
39	3498.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.902638362691874	4.1298871226028195	45.05282584072179	17.914648673983518
2	17.870220286685154	17.030963168378705	43.58424793969456	21.514568605241575
3	17.926366050853414	16.85227317111276	46.386653907745185	18.834706870288638
4	18.717777213607782	3.7978947779557086	54.19213568722415	23.292192321212358
5	24.920419482091944	4.789233683552709	44.94175487247588	25.348591961879468
6	19.65834081943522	14.491465843846424	45.952378627504594	19.897814709213765
7	66.21318790766708	1.7356364488536498	24.843280084365112	7.207895559114166
8	62.56566613287505	10.73726711713471	14.689928914580323	12.007137835409914
9	57.68366988243565	4.7160000781158455	17.7213119556302	19.879018083818302
10	32.58797797133148	23.803606999179785	24.820333554661563	18.788081474827166
11	26.13487677225325	22.39605710268328	29.96475022458306	21.50431590048041
12	20.636253564035464	18.265681756044213	36.1810627660821	24.91700191383822
13	20.57839901574034	22.2095555208374	39.4773073467953	17.734738116626957
14	18.3733351560364	26.464183884701008	29.765310705776667	25.397170253485918
15	12.450933484357302	24.85572979728938	41.01545717298754	21.677879545365776
16	14.359889466078194	25.087147990469866	29.029312971136196	31.523649572315744
17	14.400167949068468	25.997685818068195	32.80597000351521	26.796176229348124
18	14.683337890091005	22.920897941647464	36.00359332890677	26.392170839354762
19	16.206840995195876	25.019773073467956	31.179207514744366	27.59417841659181
20	17.796986681248292	25.755770808108423	37.8500566339882	18.59718587665508
21	20.685320079678164	27.855378275983284	30.35703823770652	21.102263406632034
22	16.119693004726006	23.967406163340232	31.592489161426396	28.32041167050736
23	17.070753427332736	28.14391868140452	28.927518259578953	25.857809631683786
24	21.847293285943053	22.406065695426317	27.20921376401203	28.537427254618596
25	14.955522790298012	30.23766746084443	24.387034722493457	30.4197750263641
26	15.614381127211654	26.42683474592821	29.395236886302385	28.563547240557746
27	18.302054446744524	26.19492832871148	27.22019880482756	28.28281841971644
28	12.572745381400619	24.319659805491543	37.14774635784869	25.95984845525915
29	17.153263289458266	22.871831426004764	32.61312150919814	27.36178377533883
30	17.174989259071204	22.95214427996719	34.425165019724254	25.447701441237353
31	22.05234738116627	23.069562160684296	30.07704175291958	24.801048705229856
32	18.94504550248018	26.742715697379214	28.888704448697418	25.423534351443188
33	21.53800335898137	18.868638440807718	29.20751474436589	30.38584345584502
34	24.034781080342146	22.208334960746786	29.5863765964926	24.170507362418466
35	17.538472054056164	20.146808967699098	27.46479904698668	34.84991993125806
36	23.184294809202047	23.767478420497596	29.709897277662773	23.338329492637584
37	17.76207866265672	19.382494238956372	37.05229855876265	25.803128539624264
38	18.698492364176072	21.86145178299418	34.60946959340702	24.830586259422724
39	24.69290708120142	19.39445572784439	36.275045893059406	19.637591297894776
40	21.775280240596807	18.99167089794165	33.5229270007421	25.710121860719447
41	25.68424598679842	21.778453696832404	24.48223840956138	28.055061906807794
42	21.170126547670197	17.992520407764715	30.55769831660352	30.27965472796157
43	27.559514510018357	18.23223840956138	27.450640549935557	26.757606530484708
44	19.676649220794438	17.68152169667617	33.02493848377143	29.616890598757955
45	19.203804241690428	15.678826699996092	31.68696051243995	33.43040854587353
46	26.026735148224816	18.809319220403857	32.42320235909854	22.74074327227278
47	15.161797445611844	17.66882787173378	42.73278522048197	24.4365894621724
48	20.116783189469984	19.180613599968755	31.34666835917666	29.355934851384603
49	19.58437487794399	14.483898371284615	40.80063859703941	25.131088153731984
50	24.163428113892905	14.212934031168222	32.84356325430613	28.780074600632737
51	19.568507596766004	14.561525993047688	29.096687888138106	36.773278522048194
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	83.0
1	218.5
2	354.0
3	1076.5
4	1799.0
5	1282.5
6	766.0
7	754.5
8	743.0
9	882.0
10	1021.0
11	1035.5
12	1050.0
13	1040.0
14	1030.0
15	999.5
16	969.0
17	903.0
18	837.0
19	935.0
20	1033.0
21	977.5
22	922.0
23	1290.0
24	1658.0
25	1753.5
26	2053.0
27	2257.0
28	2675.0
29	3093.0
30	3422.0
31	3751.0
32	4690.0
33	5629.0
34	6037.5
35	6446.0
36	7133.5
37	7821.0
38	8961.5
39	10102.0
40	11575.5
41	13049.0
42	15540.0
43	18031.0
44	22082.0
45	26133.0
46	55473.5
47	84814.0
48	75774.0
49	66734.0
50	63147.5
51	59561.0
52	44428.0
53	29295.0
54	23672.5
55	18050.0
56	14634.5
57	11219.0
58	9789.5
59	8360.0
60	7487.0
61	6614.0
62	5693.5
63	4773.0
64	4068.0
65	3363.0
66	2882.0
67	2401.0
68	1944.5
69	1488.0
70	1197.5
71	907.0
72	854.5
73	802.0
74	575.0
75	313.0
76	278.0
77	189.0
78	100.0
79	73.5
80	47.0
81	47.5
82	48.0
83	28.0
84	8.0
85	7.0
86	6.0
87	3.0
88	0.0
89	0.0
90	0.0
91	2.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	409648.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.70223723085452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.46489472915952	22.7165746664931
2	12.857598460142578	8.409444701253047
3	5.406361052295243	5.30400305063434
4	2.8361634223306744	3.709955562501198
5	1.716881453805263	2.807293229979705
6	1.2195960050148362	2.393011072907857
7	0.9194793504799397	2.1048322293786907
8	0.7014104516714095	1.83501527894074
9	0.614233441866265	1.8078126957931397
>10	4.064404430639393	21.98928493778378
>50	0.10361831682300607	2.362163658431806
>100	0.07733830893311323	5.017265076168098
>500	0.006757716314543875	1.6812613897383137
>1k	0.007508573682826527	6.916572342069347
>5k	0.0030034294731306112	8.078747040131482
>10k+	7.508573682826528E-4	2.866763067795357
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	11675	2.85000781158458	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	9986	2.4377026129750416	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	9658	2.3576338710307385	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	6875	1.6782701245947742	No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	6382	1.5579228996601961	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	4583	1.1187653790571417	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTTTGCGT	3970	0.9691247119478186	No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	3783	0.9234757645588407	No Hit
GAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	3472	0.8475569269226263	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTA	2926	0.7142717650275359	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC	2367	0.577813146896848	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT	2309	0.5636546498457212	No Hit
TGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT	2219	0.5416845682146623	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	1486	0.36275045893059404	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1053	0.2570499550833887	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGT	996	0.24313557005038472	No Hit
TCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	936	0.22848884896301214	No Hit
TGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	844	0.20603054329570752	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATG	786	0.1918720462445807	No Hit
TTCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	781	0.19065148615396635	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTAT	678	0.16550794828731008	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCC	630	0.15379057141741204	No Hit
TTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG	627	0.1530582353630434	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	569	0.13889973831191657	No Hit
CGTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	499	0.12181189704331523	No Hit
TGCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	489	0.11937077686208647	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACTTTGCG	478	0.11668554466273484	No Hit
TTTCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	421	0.10277115962973091	No Hit
GTTCAGGGGAAATGTAATACTGGAATAATTTGTAAATGATTTTAATTTTAT	414	0.10106237550287075	No Hit
TTGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	411	0.10033003944850212	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2652325899308674	0.0
2	0.0	0.0	0.0	5.7283326172714135	0.0
3	0.0	0.0	0.0	7.253544506503144	0.0
4	0.0	0.0	0.0	10.03788618521267	0.0
5	0.0	0.0	0.0	15.20476116080147	0.0
6	0.0	0.0	0.0	16.618413857751044	0.0
7	0.0	0.0	0.0	18.62184119048549	0.0
8	0.0	0.0	0.0	21.17134710776081	0.0
9	0.0	0.0	0.0	22.00669843377729	0.0
10	0.0	0.0	0.0	26.163437878373628	0.0
11	0.0	0.0	0.0	29.265857516697263	0.0
12	0.0	0.0	0.0	35.0083486310198	0.0
13	0.0	0.0	0.0	36.21328555247432	0.0
14	0.0	0.0	0.0	36.67856305901652	0.0
15	0.0	0.0	0.0	37.95697769792602	0.0
16	0.0	0.0	0.0	39.29397922118502	0.0
17	0.0	0.0	0.0	40.94832636800375	0.0
18	0.0	0.0	0.0	42.634408077178456	0.0
19	0.0	0.0	0.0	44.76965589969925	0.0
20	0.0	0.0	0.0	46.12935984064367	0.0
21	0.0	0.0	0.0	47.62601062375503	0.0
22	0.0	0.0	0.0	49.1004472132172	0.0
23	0.0	0.0	0.0	50.39643791743155	0.0
24	0.0	0.0	0.0	51.40291176815217	0.0
25	0.0	0.0	0.0	52.254374487364764	0.0
26	0.0	0.0	0.0	53.03821817755732	0.0
27	0.0	0.0	0.0	53.84061438112721	0.0
28	0.0	0.0	0.0	54.621528727102294	0.0
29	0.0	0.0	0.0	55.44394211615826	0.0
30	0.0	0.0	0.0	56.32518650158185	0.0
31	0.0	0.0	0.0	57.10878607975628	0.0
32	0.0	0.0	0.0	57.85991875952037	0.0
33	0.0	0.0	0.0	58.55002343475374	0.0
34	0.0	0.0	0.0	59.23426942155216	0.0
35	0.0	0.0	0.0	59.95562043510526	0.0
36	0.0	0.0	0.0	60.667206967933446	0.0
37	0.0	0.0	0.0	61.36658789985549	0.0
38	0.0	0.0	0.0	62.00860250751865	0.0
39	0.0	0.0	0.0	62.65525524352615	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCG	20	7.027965E-4	45.0	25
CGGGTTC	20	7.027965E-4	45.0	6
GCAAGCC	20	7.027965E-4	45.0	44
GGTCGCT	20	7.027965E-4	45.0	35
GACACGA	40	6.7957444E-9	45.0	25
TCGGGTA	25	3.8863946E-5	45.0	5
CATTCTA	20	7.027965E-4	45.0	45
AGGCGCG	20	7.027965E-4	45.0	10
CCGCTAC	20	7.027965E-4	45.0	34
CCGTCGC	20	7.027965E-4	45.0	22
ATTCCGG	25	3.8863946E-5	45.0	4
AGTGCGG	20	7.027965E-4	45.0	21
ACTAGGG	20	7.027965E-4	45.0	3
TATCTTG	20	7.027965E-4	45.0	1
GCTTAGA	20	7.027965E-4	45.0	14
AGACACG	40	6.7957444E-9	45.0	24
ATTGGTG	20	7.027965E-4	45.0	12
GGACCCA	40	6.7957444E-9	45.0	8
ATACGAA	40	6.7957444E-9	45.0	19
GCTAATT	20	7.027965E-4	45.0	29
>>END_MODULE