##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933761.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347227
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.421487960325667	31.0	25.0	33.0	16.0	34.0
2	28.815555817951946	31.0	28.0	33.0	16.0	34.0
3	29.700184605459828	31.0	30.0	34.0	16.0	34.0
4	32.92841858496027	35.0	32.0	37.0	28.0	37.0
5	33.915309005348085	35.0	33.0	37.0	28.0	37.0
6	33.20348648002604	35.0	33.0	37.0	28.0	37.0
7	33.51294110193044	35.0	32.0	35.0	29.0	37.0
8	33.90554881964824	35.0	35.0	37.0	28.0	37.0
9	34.86158910453392	37.0	33.0	39.0	29.0	39.0
10	33.54084791793265	35.0	31.0	39.0	27.0	39.0
11	33.73439565471579	35.0	32.0	39.0	27.0	39.0
12	33.99775939083078	37.0	32.0	39.0	26.0	39.0
13	34.305483156551766	35.0	33.0	39.0	27.0	39.0
14	34.10798699409896	37.0	32.0	39.0	25.0	40.0
15	34.68025239972698	37.0	32.0	39.0	27.0	40.0
16	34.37678521543543	36.0	32.0	39.0	27.0	40.0
17	34.329585544902905	36.0	32.0	38.0	27.0	40.0
18	34.130568763373816	36.0	32.0	39.0	25.0	40.0
19	33.90035337113761	36.0	32.0	39.0	25.0	40.0
20	35.0741935390969	37.0	33.0	39.0	28.0	40.0
21	34.133753999544965	37.0	32.0	39.0	25.0	40.0
22	35.19215959588396	37.0	33.0	39.0	27.0	40.0
23	36.055623554619885	38.0	34.0	39.0	30.0	40.0
24	36.20476230247072	38.0	35.0	40.0	31.0	40.0
25	34.06177802993431	37.0	32.0	39.0	24.0	40.0
26	34.77530261183606	37.0	33.0	39.0	27.0	40.0
27	35.47006713187626	37.0	34.0	39.0	29.0	40.0
28	35.65500378714789	38.0	34.0	40.0	30.0	40.0
29	36.112001658857174	38.0	35.0	40.0	30.0	41.0
30	34.00099070636788	37.0	32.0	39.0	24.0	40.0
31	34.70647155895135	37.0	33.0	39.0	26.0	40.0
32	35.11826557266572	37.0	34.0	39.0	29.0	40.0
33	36.03684333303574	38.0	35.0	40.0	30.0	40.0
34	36.28321530295743	38.0	35.0	40.0	31.0	41.0
35	36.44899446183621	38.0	35.0	40.0	31.0	41.0
36	36.38774058468955	38.0	35.0	40.0	31.0	41.0
37	36.600641655170826	38.0	35.0	40.0	31.0	41.0
38	36.09530940854254	38.0	35.0	40.0	30.0	41.0
39	35.97397955804128	38.0	35.0	40.0	30.0	41.0
40	35.61634607907795	38.0	34.0	40.0	30.0	41.0
41	35.04290276965789	37.0	33.0	39.0	28.0	41.0
42	36.0239324706892	38.0	35.0	40.0	30.0	41.0
43	36.09571548295496	38.0	35.0	40.0	31.0	41.0
44	36.52613996031415	38.0	35.0	40.0	32.0	41.0
45	36.754451698744624	39.0	35.0	40.0	32.0	41.0
46	36.529140879021504	38.0	35.0	40.0	32.0	41.0
47	36.437198144153534	38.0	35.0	40.0	31.0	41.0
48	36.47939820348072	38.0	35.0	40.0	32.0	41.0
49	36.65191358966901	38.0	35.0	40.0	32.0	41.0
50	36.49620277224985	38.0	35.0	40.0	32.0	41.0
51	35.76341125546112	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	7.0
11	10.0
12	14.0
13	11.0
14	25.0
15	33.0
16	50.0
17	100.0
18	155.0
19	329.0
20	519.0
21	706.0
22	995.0
23	1340.0
24	1779.0
25	2370.0
26	3382.0
27	4418.0
28	5888.0
29	8160.0
30	11617.0
31	16374.0
32	22691.0
33	29397.0
34	34905.0
35	45046.0
36	56691.0
37	60671.0
38	35836.0
39	3699.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.247725551296416	4.199846210116149	49.066172849461594	17.486255389125844
2	18.67971096717709	11.239045350735974	49.840306197386724	20.24093748470021
3	19.09240928844819	11.527905376022025	48.97891005019771	20.400775285332074
4	18.982106806210346	3.8882344978933085	53.23952342415769	23.89013527173866
5	22.540297845501648	5.270039484256697	48.33783087144721	23.85183179879445
6	21.405017466959656	12.203256083196296	48.698977902064065	17.69274854777998
7	77.16191425205989	1.7006165995155906	16.587995749178493	4.549473399246026
8	76.44365213534662	7.49365688728123	9.128610390321029	6.934080587051122
9	72.04969659617484	3.9775132694174125	11.132486816981398	12.840303317426352
10	37.70818513537254	27.05722769254695	18.499713443943012	16.7348737281375
11	32.60403136852837	23.452957287307726	24.694796199604294	19.24821514455961
12	26.586930163840943	20.54794126032826	30.3864618822845	22.478666693546298
13	25.471233515826825	22.23300607383642	32.910171156044896	19.38558925429186
14	21.373913894944803	25.575776077321176	28.71723685082093	24.333073176913086
15	17.641773249200092	23.56441175369427	37.70559317103797	21.088221826067674
16	20.556581141443495	22.376140104312164	30.53103589294611	26.536242861298227
17	20.044524187347182	24.2786419258871	31.15339532928027	24.52343855748545
18	20.530373502060613	23.07280251823735	30.958134016075938	25.4386899636261
19	21.66046995193346	25.41997022120976	28.55797504226342	24.361584784593365
20	24.44855958782007	25.08387884582708	32.355778784483924	18.11178278186892
21	24.886889556399705	27.7757778052974	27.64301163215994	19.694321006142957
22	21.985617477903503	23.223136449642453	29.445002836760963	25.346243235693077
23	21.189308435115933	27.369127400806963	27.317576110152725	24.12398805392438
24	22.91325271364266	22.969987932966042	27.9284157049999	26.1883436483914
25	18.843868708366575	27.742658261022328	26.169911902012228	27.24356112859887
26	19.206167723132133	26.355381349952623	28.327578212523797	26.110872714391448
27	21.106941568484018	26.184311703870954	26.23672698263672	26.472019745008307
28	18.94927525797245	25.50867300065951	31.943944451324352	23.59810729004369
29	20.669763584053083	25.87989989257748	27.449190299141485	26.001146224227956
30	22.509194273486795	24.939016839128296	27.95577532853148	24.59601355885343
31	25.000071999009293	25.827772609848886	25.807036895172324	23.365118495969494
32	23.314719189463954	29.98125145797994	24.308017521678902	22.396011830877207
33	23.14019359093619	22.857669478467976	26.682256852145713	27.31988007845012
34	22.58464923522652	25.45971367433984	28.747476434724256	23.208160655709378
35	18.40467475167542	24.754123383262247	26.412116569276005	30.42908529578633
36	23.505084570036317	26.352213393543707	27.080555371558084	23.062146664861892
37	19.943725574336096	26.13650436169998	29.581800954418867	24.337969109545053
38	25.21491704274149	26.255734721090242	23.978550055151242	24.55079818101703
39	22.93226045209618	26.94865318653215	29.185806403303893	20.93327995806778
40	24.61070135674933	22.278221451672824	28.363289721133437	24.747787470444408
41	19.315894213295625	23.782712749872562	30.003139156805204	26.898253880026612
42	20.394727368551408	21.70568532976986	29.197902236865218	28.70168506481351
43	22.56362552451278	22.437771256267514	28.741140521906416	26.257462697313283
44	21.20428422904901	22.448715105680144	29.013872769110698	27.333127896160146
45	21.99569733920461	22.102831865033536	26.268694542763093	29.63277625299876
46	24.280369902110145	24.576429828325562	27.47395795833849	23.669242311225798
47	18.275652527021226	22.75053495263905	34.358503226995595	24.615309293344122
48	20.986559224945065	22.856229498282104	29.38769162536323	26.769519651409595
49	20.955167656893043	20.295080739689023	33.73383982236404	25.01591178105389
50	22.413003597070507	21.256987503851946	29.684903535727347	26.6451053633502
51	20.250153357889793	20.261673199376776	27.533861134070797	31.95431230866263
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	40.0
1	187.0
2	334.0
3	717.5
4	1101.0
5	817.0
6	533.0
7	562.0
8	591.0
9	608.0
10	625.0
11	700.5
12	776.0
13	779.0
14	782.0
15	832.5
16	883.0
17	839.0
18	795.0
19	748.0
20	701.0
21	823.0
22	945.0
23	1013.0
24	1081.0
25	1182.5
26	1617.0
27	1950.0
28	2298.0
29	2646.0
30	3263.0
31	3880.0
32	4415.5
33	4951.0
34	5740.0
35	6529.0
36	7656.5
37	8784.0
38	9600.5
39	10417.0
40	11391.5
41	12366.0
42	14508.0
43	16650.0
44	19463.0
45	22276.0
46	37039.0
47	51802.0
48	47412.0
49	43022.0
50	40859.5
51	38697.0
52	32502.5
53	26308.0
54	23031.0
55	19754.0
56	17847.5
57	15941.0
58	14788.0
59	13635.0
60	12333.5
61	11032.0
62	10110.5
63	9189.0
64	7694.5
65	6200.0
66	4957.5
67	3715.0
68	3067.5
69	2420.0
70	1997.0
71	1574.0
72	1410.0
73	1246.0
74	1071.0
75	737.0
76	578.0
77	364.5
78	151.0
79	136.5
80	122.0
81	68.5
82	15.0
83	10.0
84	5.0
85	4.0
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	347227.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.636134876857977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.1243530535749	20.485786503712955
2	12.986671731858385	7.6974951009366785
3	5.140299531081706	4.570158306317618
4	2.5074552715961844	2.9724513050685233
5	1.4108427292026104	2.0905962706341494
6	0.900611832040491	1.6014392235627692
7	0.6499972679606444	1.3484384692009599
8	0.5012267020510935	1.1883537716695132
9	0.4293376069344555	1.1451516504135264
>10	6.101586305487507	40.950267860687596
>50	0.18571347615901737	3.503217148639449
>100	0.04792605836361739	2.625563456362685
>500	0.0029953786477260867	0.6140024988112894
>1k	0.008986135943178259	5.692320997413386
>5k	0.001996919098484058	3.514757436568914
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGC	6748	1.943397258853718	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG	5130	1.477419670705331	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC	4428	1.275246452608812	No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	3043	0.87637194112209	No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	2503	0.7208540810478448	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGAGTCTT	2038	0.5869359237616891	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	1925	0.5543923715609673	No Hit
TCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	1558	0.4486978259179154	No Hit
GAACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT	1440	0.414714293531321	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTA	1234	0.35538710987336813	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTC	1068	0.3075797677023964	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCT	895	0.257756453271203	No Hit
TGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTT	669	0.19266934886975956	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	511	0.14716597499618406	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGT	495	0.1425580384013916	No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG	442	0.1272942484311416	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATG	397	0.11433442675828781	No Hit
TCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG	362	0.1042545654571793	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6379112223415806	0.0
2	0.0	0.0	0.0	2.848280807656087	0.0
3	0.0	0.0	0.0	3.5881426271574504	0.0
4	0.0	0.0	0.0	4.959003764108206	0.0
5	0.0	0.0	0.0	7.731829610024566	0.0
6	0.0	0.0	0.0	8.539658494299118	0.0
7	0.0	0.0	0.0	9.537276767071685	0.0
8	0.0	0.0	0.0	10.956521238267761	0.0
9	0.0	0.0	0.0	11.398307159293488	0.0
10	0.0	0.0	0.0	13.972991731633773	0.0
11	0.0	0.0	0.0	15.909765081632477	0.0
12	0.0	0.0	0.0	19.705840847629936	0.0
13	0.0	0.0	0.0	20.48717409648443	0.0
14	0.0	0.0	0.0	20.756738387279793	0.0
15	0.0	0.0	0.0	21.46319266646891	0.0
16	0.0	0.0	0.0	22.298093178237867	0.0
17	0.0	0.0	0.0	23.388446174980633	0.0
18	0.0	0.0	0.0	24.481967128132318	0.0
19	0.0	0.0	0.0	25.874139971833987	0.0
20	0.0	0.0	0.0	26.8429586408891	0.0
21	0.0	0.0	0.0	27.96671917794411	0.0
22	2.879960371745285E-4	0.0	0.0	29.11150342571286	0.0
23	2.879960371745285E-4	0.0	0.0	30.194656521526262	0.0
24	2.879960371745285E-4	0.0	0.0	31.069588482462482	0.0
25	2.879960371745285E-4	0.0	0.0	31.79908244462556	0.0
26	2.879960371745285E-4	0.0	0.0	32.46953721916786	0.0
27	2.879960371745285E-4	0.0	0.0	33.070584948751105	0.0
28	2.879960371745285E-4	0.0	0.0	33.70705619090681	0.0
29	2.879960371745285E-4	0.0	0.0	34.39680669993981	0.0
30	2.879960371745285E-4	0.0	0.0	35.11679679287613	0.0
31	2.879960371745285E-4	0.0	0.0	35.83131496110614	0.0
32	2.879960371745285E-4	0.0	0.0	36.45799433799791	0.0
33	2.879960371745285E-4	0.0	0.0	37.1206732195365	0.0
34	2.879960371745285E-4	0.0	0.0	37.76693632695614	0.0
35	2.879960371745285E-4	0.0	0.0	38.427311240197334	0.0
36	2.879960371745285E-4	0.0	0.0	39.07155837535676	0.0
37	2.879960371745285E-4	0.0	0.0	39.72559737578011	0.0
38	2.879960371745285E-4	0.0	0.0	40.33902893496185	0.0
39	2.879960371745285E-4	0.0	0.0	40.97838013748931	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGAT	60	0.0	45.000004	5
TTTACGG	30	2.1611104E-6	45.000004	2
TTATCCG	35	1.2086457E-7	45.000004	1
GTAGCTT	30	2.1611104E-6	45.000004	32
TATCCGG	35	1.2086457E-7	45.000004	2
CCATTAG	30	2.1611104E-6	45.000004	21
ATTTACG	20	7.026479E-4	45.0	35
CTTCCCG	20	7.026479E-4	45.0	1
CATGCGC	20	7.026479E-4	45.0	18
CCCTAGA	20	7.026479E-4	45.0	11
ACGCACC	25	3.8851635E-5	45.0	11
ACGCAAA	20	7.026479E-4	45.0	23
GCGCATT	20	7.026479E-4	45.0	10
TATGCAT	25	3.8851635E-5	45.0	34
TCGCTTG	50	2.1827873E-11	45.0	1
TCCACGG	20	7.026479E-4	45.0	2
CGAATTC	20	7.026479E-4	45.0	17
CGAATGT	20	7.026479E-4	45.0	40
AACGAGG	25	3.8851635E-5	45.0	12
AACGACG	20	7.026479E-4	45.0	11
>>END_MODULE