FastQCFastQC Report
Sat 14 Jan 2017
SRR2933760.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933760.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384482
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC217051.5677343584098602No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG193981.4011016394579345No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC168551.2174228339552264No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC153091.1057565212115432TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC132560.9574700140557986TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT90400.6529517899113171Illumina PCR Primer Index 8 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC73450.5305233293029451TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGCGTAT72590.5243116197971516No Hit
GAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT55420.4002941172221813No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTA49040.3542119001908295No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTC46090.3329042920023518No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT39510.2853774913650015No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG35940.2595916739979285No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31210.2254272717160642No Hit
TGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTT25120.18143970091341022TruSeq Adapter, Index 14 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC22580.1630934891172294No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT20230.14611963174674716No Hit
TCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG18660.134779650439659No Hit
TGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG16770.1211283353629733No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGT16630.12011712683877437No Hit
TTCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT16220.11715573044647745No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG15650.11303866716938177No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC14200.10256543602589273No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG302.1658252E-644.9999969
TACGGCT27550.041.1615227
TCGTTCA1150.041.08695616
CGTTTTT21150.040.6382941
TTTAGCG900.040.01
ACGGCTG28600.039.8863648
TTTGTCG3000.039.7500041
TGATACC23300.039.5922744
GCCGATG1200.039.3749969
TTGGGAC22300.039.248885
ATGATAC82300.039.2041323
TTTACGG750.039.0000042
GAATGAT85500.038.947371
TTTTGCG12050.038.8381731
AATGATA82400.038.7742772
GATACCT23800.038.7605065
ATACGGC60150.038.7531176
CGGGCTA1050.038.571436
GTTGATC1400.038.57142616
AGGCACG3450.038.4782610
Produced by FastQC (version 0.11.5)