##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933760.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1384482
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.49633003534896	31.0	25.0	33.0	16.0	34.0
2	28.868329815772253	31.0	28.0	33.0	16.0	34.0
3	29.750341282876917	31.0	30.0	34.0	16.0	34.0
4	33.02188544163088	35.0	32.0	37.0	28.0	37.0
5	33.97476962502943	35.0	33.0	37.0	28.0	37.0
6	33.42297552441996	35.0	33.0	37.0	28.0	37.0
7	33.70160175430233	35.0	33.0	36.0	30.0	37.0
8	33.95390911546701	35.0	35.0	37.0	30.0	37.0
9	34.911512753506365	37.0	34.0	39.0	29.0	39.0
10	33.60711587438479	35.0	31.0	39.0	27.0	39.0
11	33.91424446110531	35.0	32.0	39.0	27.0	39.0
12	33.978838294755725	37.0	32.0	39.0	26.0	39.0
13	34.319843089328714	37.0	33.0	39.0	27.0	39.0
14	34.18763335312413	37.0	32.0	39.0	25.0	40.0
15	34.58142395495211	37.0	32.0	39.0	27.0	40.0
16	34.204984246815776	36.0	32.0	39.0	27.0	40.0
17	34.22261250055978	36.0	32.0	38.0	27.0	40.0
18	34.2719638102915	36.0	32.0	39.0	25.0	40.0
19	33.896138772479524	36.0	32.0	39.0	25.0	40.0
20	35.04051262493842	37.0	33.0	39.0	27.0	40.0
21	34.20391525494734	37.0	32.0	39.0	25.0	40.0
22	35.12955459153676	37.0	33.0	39.0	27.0	40.0
23	36.16684073899119	38.0	34.0	39.0	30.0	40.0
24	36.270638404833	38.0	35.0	40.0	31.0	40.0
25	34.08985887862753	37.0	32.0	39.0	24.0	40.0
26	34.84907279401249	37.0	33.0	39.0	27.0	40.0
27	35.49947778302643	37.0	34.0	39.0	30.0	40.0
28	35.77036610082327	38.0	34.0	40.0	30.0	40.0
29	36.25831249521482	38.0	35.0	40.0	30.0	41.0
30	34.05877432859366	37.0	32.0	39.0	24.0	40.0
31	34.745377693606706	37.0	33.0	39.0	26.0	40.0
32	35.082324652830444	37.0	33.0	39.0	29.0	40.0
33	35.989526768856514	38.0	35.0	40.0	30.0	40.0
34	36.26418111611419	38.0	35.0	40.0	31.0	41.0
35	36.317848841660634	38.0	35.0	40.0	31.0	41.0
36	36.235118260836906	38.0	35.0	40.0	30.0	41.0
37	36.38121477924596	38.0	35.0	40.0	31.0	41.0
38	35.95943537005176	38.0	34.0	40.0	30.0	41.0
39	35.89628756459094	38.0	34.0	40.0	30.0	41.0
40	35.376064838690574	37.0	34.0	40.0	28.0	41.0
41	34.659032042308965	37.0	33.0	39.0	26.0	40.0
42	35.584070432118295	37.0	34.0	40.0	30.0	41.0
43	35.70224892775782	38.0	34.0	40.0	30.0	41.0
44	36.083660892665996	38.0	35.0	40.0	31.0	41.0
45	36.27897365223961	38.0	35.0	40.0	31.0	41.0
46	35.9658998816886	37.0	35.0	40.0	31.0	41.0
47	35.77945036482959	37.0	34.0	40.0	30.0	41.0
48	35.62983772992354	37.0	34.0	40.0	30.0	41.0
49	35.648739384116226	37.0	34.0	39.0	31.0	41.0
50	35.44553414201123	36.0	34.0	39.0	30.0	41.0
51	34.505979131545224	35.0	33.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	15.0
10	39.0
11	42.0
12	42.0
13	61.0
14	57.0
15	127.0
16	201.0
17	401.0
18	716.0
19	1164.0
20	1894.0
21	2518.0
22	3618.0
23	5085.0
24	7197.0
25	10247.0
26	14162.0
27	18836.0
28	25598.0
29	34681.0
30	49244.0
31	69127.0
32	95081.0
33	119966.0
34	145146.0
35	187461.0
36	231096.0
37	226527.0
38	121366.0
39	12759.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.357609560832138	3.6616582953046697	50.462483441460414	17.518248702402776
2	19.460491360667746	10.869119280712932	48.10051701647259	21.569872342146738
3	19.619901161589677	10.890065742999909	49.17239805212347	20.31763504328695
4	19.299420288598913	3.923200157170696	52.239032360117356	24.538347194113033
5	23.093185754672145	4.678139549665507	47.04373187950439	25.184942816157957
6	20.853358873571487	10.840660983674761	49.048091632827294	19.257888509926456
7	74.95763758575409	1.7487406842414708	17.949673596334225	5.34394813367021
8	74.16376666507762	6.862422191115522	10.842683400723159	8.131127743083695
9	69.13834921653009	3.9790333135425375	13.588042314742987	13.294575155184393
10	38.52357777132531	21.82787497417807	22.20967842124347	17.43886883325316
11	30.484397774763412	22.62817429190123	26.51251515007057	20.374912783264786
12	25.312282861026723	19.0256716952622	33.00165693739608	22.660388506314995
13	25.283752334808252	21.46196194677865	35.3764801564773	17.8778055619358
14	20.60272361793075	23.272458580176558	33.35319635791581	22.77162144397688
15	17.196251016625713	23.1317561369523	38.34473832090269	21.327254525519294
16	21.517145040527794	23.748882253434857	28.44717374440404	26.286798961633306
17	21.610392912294994	24.152137767049336	30.64366311732475	23.59380620333092
18	22.213000963537265	21.266870930788556	33.38194357167518	23.138184533999
19	22.00815900820668	24.142097910987648	29.18701723821617	24.662725842589502
20	21.201720210158022	27.498660148705433	33.195520057321076	18.104099583815465
21	23.138617909080796	26.470044392054216	31.218824080053043	19.172513618811944
22	19.664466565834733	22.652948900744104	32.78403041715241	24.898554116268755
23	19.30245391417151	25.425610444917307	31.123337103696546	24.148598537214642
24	22.607372287974854	24.057517540856434	27.861828467253456	25.47328170391526
25	21.131946821988297	26.943506668920218	24.84633241891191	27.078214090179575
26	21.325232108470892	23.510959333526905	27.981801135731633	27.182007422270566
27	21.30016858290682	25.836305564102673	28.885243722923086	23.97828213006742
28	20.218247691194254	23.542451256137674	29.737620279642496	26.501680773025576
29	26.05487106369025	23.072167063204866	25.938076479145266	24.93488539395962
30	25.215856905326323	24.118334510668973	27.48580335461205	23.180005229392652
31	28.86603076096331	23.207307859546024	25.581625474365143	22.345035905125528
32	27.78273751482504	25.172013792884268	25.414631609511716	21.630617082778976
33	23.74967677441816	24.15676043458853	24.100710590675792	27.992852200317518
34	27.347340015977096	21.561349298871345	28.656277221372328	22.435033463779234
35	22.16077926618042	25.49957312554443	27.147120728185705	25.19252688008945
36	27.913905706249704	26.8654991541963	23.895724176984608	21.324870962569396
37	24.376553830241203	26.802154163073265	28.647537490556036	20.1737545161295
38	25.647354028438073	25.090033673243855	25.461725035067268	23.800887263250807
39	26.93180554171163	21.95702074855433	26.58640560151739	24.524768108216648
40	25.13293780634201	20.675964006754874	29.30641207325195	24.88468611365117
41	23.95885248056674	24.198508900801887	25.87855963457813	25.96407898405324
42	21.81588493024828	22.71701618367014	31.965168200092165	23.501930685989418
43	24.209704423748377	23.363900722436263	27.709280438460016	24.717114415355347
44	24.24574678471804	20.837179537184305	28.168369108446335	26.74870456965132
45	24.124257303453568	18.13241342249303	26.4847069156551	31.258622358398302
46	26.624253691994547	20.903847070601135	28.4886332938962	23.983265943508115
47	19.286780182046424	22.24572078221313	35.25657971717942	23.21091931856102
48	22.053518933435033	24.423575026616454	28.101990491750705	25.420915548197808
49	23.897024302230005	19.546588543585255	33.53716408013972	23.019223074045023
50	24.464312284305613	19.746157768753946	28.181875965162423	27.607653981778025
51	21.648602148673657	19.18825958011733	26.194562298390302	32.96857597281871
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	209.0
1	763.5
2	1318.0
3	3405.0
4	5492.0
5	3827.5
6	2163.0
7	2128.5
8	2094.0
9	2322.5
10	2551.0
11	2580.0
12	2609.0
13	2609.5
14	2610.0
15	2439.0
16	2268.0
17	2428.5
18	2589.0
19	2788.5
20	2988.0
21	3160.5
22	3333.0
23	3670.5
24	4008.0
25	5190.0
26	7154.5
27	7937.0
28	9768.0
29	11599.0
30	12990.5
31	14382.0
32	16455.0
33	18528.0
34	22080.5
35	25633.0
36	28327.5
37	31022.0
38	33847.5
39	36673.0
40	39697.0
41	42721.0
42	46946.5
43	51172.0
44	58394.0
45	65616.0
46	75285.0
47	84954.0
48	146496.5
49	208039.0
50	199569.0
51	191099.0
52	187529.0
53	183959.0
54	149541.5
55	115124.0
56	97305.5
57	79487.0
58	67910.0
59	56333.0
60	46438.0
61	36543.0
62	31126.5
63	25710.0
64	22662.5
65	19615.0
66	16519.5
67	13424.0
68	11002.5
69	8581.0
70	7161.5
71	5742.0
72	4721.0
73	3700.0
74	3207.5
75	2261.0
76	1807.0
77	1318.5
78	830.0
79	631.5
80	433.0
81	383.0
82	333.0
83	225.5
84	118.0
85	71.5
86	25.0
87	22.0
88	19.0
89	11.5
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1384482.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.10981216013441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.14670473670014	22.202877005877177
2	12.825325558318301	8.236375891403432
3	5.155112183373696	4.965890516176491
4	2.6137067185270797	3.357025270943433
5	1.644613721265096	2.640411883290094
6	1.0772056707492432	2.0753323047353875
7	0.7983451862364879	1.7944299778300803
8	0.6785059772243711	1.7429359582562394
9	0.5724401678660741	1.6542851636785902
>10	5.26045414645284	30.292391735616413
>50	0.13315033159829404	2.915210459515962
>100	0.08100981821161682	4.987235555286091
>500	0.007054225216572744	1.5400477540707602
>1k	0.00432355739080265	3.14096089596431
>5k	9.102226085900315E-4	2.1325536722640415
>10k+	0.0011377782607375393	6.322035955091539
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	21705	1.5677343584098602	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG	19398	1.4011016394579345	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	16855	1.2174228339552264	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	15309	1.1057565212115432	TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	13256	0.9574700140557986	TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	9040	0.6529517899113171	Illumina PCR Primer Index 8 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	7345	0.5305233293029451	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGCGTAT	7259	0.5243116197971516	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	5542	0.4002941172221813	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTA	4904	0.3542119001908295	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTC	4609	0.3329042920023518	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	3951	0.2853774913650015	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	3594	0.2595916739979285	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3121	0.2254272717160642	No Hit
TGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTT	2512	0.18143970091341022	TruSeq Adapter, Index 14 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC	2258	0.1630934891172294	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	2023	0.14611963174674716	No Hit
TCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	1866	0.134779650439659	No Hit
TGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	1677	0.1211283353629733	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGT	1663	0.12011712683877437	No Hit
TTCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	1622	0.11715573044647745	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1565	0.11303866716938177	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	1420	0.10256543602589273	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7264810954566401	0.0
2	0.0	0.0	0.0	3.5147441425746235	0.0
3	0.0	0.0	0.0	4.48384305465871	0.0
4	0.0	0.0	0.0	6.005784112758418	0.0
5	0.0	0.0	0.0	9.084625152222998	0.0
6	0.0	0.0	0.0	10.290130171428736	0.0
7	0.0	0.0	0.0	11.674908016138888	0.0
8	0.0	0.0	0.0	13.531486866568146	0.0
9	0.0	0.0	0.0	14.20574626466794	0.0
10	0.0	0.0	0.0	16.98678639375593	0.0
11	0.0	0.0	0.0	19.603432908481295	0.0
12	0.0	0.0	0.0	23.350827240801976	0.0
13	7.222918029992445E-5	0.0	0.0	24.231084261117154	0.0
14	7.222918029992445E-5	0.0	0.0	24.596636142615072	0.0
15	7.222918029992445E-5	0.0	0.0	25.39447966820804	0.0
16	7.222918029992445E-5	0.0	0.0	26.360761642260428	0.0
17	7.222918029992445E-5	0.0	0.0	27.675043806997852	0.0
18	7.222918029992445E-5	0.0	0.0	28.95747290322301	0.0
19	7.222918029992445E-5	0.0	0.0	30.455289415102545	0.0
20	7.222918029992445E-5	0.0	0.0	31.52695376321252	0.0
21	7.222918029992445E-5	0.0	0.0	32.86355474466262	0.0
22	7.222918029992445E-5	0.0	0.0	34.30120434935232	0.0
23	7.222918029992445E-5	0.0	0.0	35.637733101622125	0.0
24	7.222918029992445E-5	0.0	0.0	36.64366889565917	0.0
25	7.222918029992445E-5	0.0	0.0	37.59167688709568	0.0
26	7.222918029992445E-5	0.0	0.0	38.44470350643779	0.0
27	7.222918029992445E-5	0.0	0.0	39.36995930607982	0.0
28	7.222918029992445E-5	0.0	0.0	40.21309052772084	0.0
29	1.444583605998489E-4	0.0	0.0	41.17301633390683	0.0
30	1.444583605998489E-4	0.0	0.0	42.231823887923426	0.0
31	1.444583605998489E-4	0.0	0.0	43.07769981841584	0.0
32	1.444583605998489E-4	0.0	0.0	43.86875380106061	0.0
33	1.444583605998489E-4	0.0	0.0	44.71824119056802	0.0
34	1.444583605998489E-4	0.0	0.0	45.61453308890979	0.0
35	1.444583605998489E-4	0.0	0.0	46.5600852882161	0.0
36	1.444583605998489E-4	0.0	0.0	47.373096941672046	0.0
37	1.444583605998489E-4	0.0	0.0	48.16682340398792	0.0
38	1.444583605998489E-4	0.0	0.0	49.11158108231093	0.0
39	1.444583605998489E-4	0.0	0.0	50.656418790565716	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	30	2.1658252E-6	44.999996	9
TACGGCT	2755	0.0	41.161522	7
TCGTTCA	115	0.0	41.086956	16
CGTTTTT	2115	0.0	40.638294	1
TTTAGCG	90	0.0	40.0	1
ACGGCTG	2860	0.0	39.886364	8
TTTGTCG	300	0.0	39.750004	1
TGATACC	2330	0.0	39.592274	4
GCCGATG	120	0.0	39.374996	9
TTGGGAC	2230	0.0	39.24888	5
ATGATAC	8230	0.0	39.204132	3
TTTACGG	75	0.0	39.000004	2
GAATGAT	8550	0.0	38.94737	1
TTTTGCG	1205	0.0	38.838173	1
AATGATA	8240	0.0	38.774277	2
GATACCT	2380	0.0	38.760506	5
ATACGGC	6015	0.0	38.753117	6
CGGGCTA	105	0.0	38.57143	6
GTTGATC	140	0.0	38.571426	16
AGGCACG	345	0.0	38.47826	10
>>END_MODULE